LOCUS       sequence-467247-        2346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid for MoClo, encodes the SSA3 promoter for 
            K.marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, 
            Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, 
            Martin VJJ
  TITLE     Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a 
            Host for Lactose Valorization.
  JOURNAL   ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
  PUBMED    40629255
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096."
COMMENT     Sequence Label: pMTKM20
FEATURES             Location/Qualifiers
     source          1..2346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(711..745)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(816..1475)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1390..1409
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1657..2245
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2146..2165
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgcttgcca ctatcaagtg agcgtccgcg cgctttccag agtggcctag
       61 aatgtgccgg caccctctgg tctggagcga gggcccttaa tttttattgg atacccggag
      121 aattaaggga aaagaagaag cttctcgatg ggcccacacc agcaaatgcc agtagtagtg
      181 gccgcagtgt agtagtagta ggacgcaccg tatggtagtg ccatttggta gcggtgctac
      241 tagaacacac tagccaggaa ctagccaaag gttggtgccc accggttttc gtatgtcttt
      301 tgtcactaaa tcgggtaatt gtgctactgt ttgattttga tttcgtaata tggcaatagc
      361 aagctgaaag tgtaagtagc gcagaagact gtggaatgtc atagaataaa gggagattcg
      421 tttggtctga cactggaagg gcatattgga cagtggaaca ctggggggtg agaaaaggta
      481 tataagggga gctgtagaga agagtattgg gaattgagga tggttgagaa gttcaattgc
      541 aataaagtat ttgttgtaat aaagcttatg tgttagctat acaaaagcat caagccacta
      601 acgaaaacta ttaagttaaa caaagtaaac tgtcgccaag actctttatt ttaagtgagt
      661 gaatttttac agagctaaaa aagaagaaga atttatgtga gaccagacca ataaaaaacg
      721 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
      781 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
      841 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
      901 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
      961 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     1021 ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag
     1081 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     1141 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     1201 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     1261 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     1321 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     1381 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     1441 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     1501 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     1561 cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1621 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1681 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1741 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1801 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1861 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1921 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1981 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2041 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2101 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2161 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2221 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2281 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2341 ccgtag
//
