LOCUS       sequence-467248-        2370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid for MoClo, encodes the PIR1 promoter for 
            K.marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, 
            Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, 
            Martin VJJ
  TITLE     Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a 
            Host for Lactose Valorization.
  JOURNAL   ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
  PUBMED    40629255
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096."
COMMENT     Sequence Label: pMTKM19
FEATURES             Location/Qualifiers
     source          1..2370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(735..769)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(840..1499)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1414..1433
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1681..2269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2170..2189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acggcctggg ttttcaaatt gacattaatt tttttaacca gcttgaaatt
       61 tcatggtgcg aatattctgt acggaaaaaa aaaaaaatac acacaaagca catacagatc
      121 tacgtaggac gaaaggtacc aaaggggaac gagtggctgg ttgtggttta ttttccctca
      181 gtcactcttc ttgagttgag ttctgtcgag ctctattgtc cgatgattac cttttcctca
      241 gtattcattg atcaaagaga ggctaatgct actatttcta atttttttgc tgggatcaat
      301 atatcgaatt cggcaatttg tctggtaact ttcaagcttt gattcctggt tttagtttct
      361 caggcaagag agtacaggta ccaggaaacc actggagtat gcatatacgg gtctgtctat
      421 ggatcaaaaa atgatgcttg cttcaatttt gtgcatgttg gatcacagtt tagatttcga
      481 cattgttccc agcacaattg acacacaaaa gattcattga cgcttgagat ccaaaaagta
      541 taaaagagta acaggtttca gacttttttg gatctcttga ttgatgtttc ctaatagttg
      601 tatatcatat cttttgttac tcacaaagaa catcaagaaa taagcatcga atcacatcga
      661 ctctttccta taacaaaaaa gcttcccttt tcaatccaaa catttcaatt tatacactat
      721 gtgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
      781 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
      841 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
      901 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
      961 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     1021 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc
     1081 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     1141 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     1201 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     1261 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     1321 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1381 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     1441 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1501 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1561 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc
     1621 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1681 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1741 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1801 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1861 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1921 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1981 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2041 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2101 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2161 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2221 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2281 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2341 gttatcccct gattctgtgg ataaccgtag
//
