LOCUS       sequence-467253-        2372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid for MoClo, encodes the HSP60 promoter for 
            K.marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, 
            Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, 
            Martin VJJ
  TITLE     Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a 
            Host for Lactose Valorization.
  JOURNAL   ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
  PUBMED    40629255
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096."
COMMENT     Sequence Label: pMTKM18
FEATURES             Location/Qualifiers
     source          1..2372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(737..771)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(842..1501)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1416..1435
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1683..2271
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2172..2191
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acggaagata cagagaaacg acctgactaa aacagaggtt tccccgctga
       61 aaagaatttt ctagaaaggg aggcaccagt ttgggcatga atatactgct aatagatagg
      121 gatacataca tgtactcact cactgttgtt tccattggaa atgcgagttc ggcaaaatca
      181 cggttaaata tgaaaagatg gacgaaaccg aaaaaccgcg tggagatcca tctccgcccc
      241 tcttccgctt cgaaactcac agaacactca actgaccctt aattactgct gtttctcaat
      301 ttctcaatct tgcagctttc ccagctatta gtttaacttt tttttttttt tttcttgttc
      361 tgtcaatttt gttttagcat gacggatttt ttttttctgt aattttcttt tccgtttttt
      421 ggcttttcat cgaaaaattt cagagggttt aaagtatata agaaaagagg attaagaatc
      481 tgggaaaatt ggaaaaaaga gatgggaact ttagcgccac ttgattcgga taaacagagt
      541 cagcaatcag ctatcggtcg ttctgtcgtc ttataaacta gggagccggg ttaaaaatat
      601 tgtttcaaaa ggggacacgt caggattttg attggttagt ttattttgaa ggatcagaaa
      661 ccaagtggat ttaaattaaa ttaaattaaa ttaatataat acaatacaat acacccgggt
      721 atgtgagacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
      781 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
      841 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
      901 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
      961 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     1021 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg aaaaacatat
     1081 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     1141 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     1201 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     1261 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     1321 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     1381 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     1441 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     1501 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     1561 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc
     1621 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1681 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1741 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1801 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     1861 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1921 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1981 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2041 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2101 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2161 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2221 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2281 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2341 gcgttatccc ctgattctgt ggataaccgt ag
//
