LOCUS       sequence-467258-        2391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid for MoClo, encodes the SOD1 promoter for 
            K.marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, 
            Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, 
            Martin VJJ
  TITLE     Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a 
            Host for Lactose Valorization.
  JOURNAL   ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
  PUBMED    40629255
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096."
COMMENT     Sequence Label: pMTKM21
FEATURES             Location/Qualifiers
     source          1..2391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(756..790)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(861..1520)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1435..1454
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1702..2290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2191..2210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgcaatacc acccgccaga atattttttt ttttttttgt tttgttttgt
       61 tttgttttgt ttttcgtttc ctttcctttc ctttcccgtg gcgctcggca ttgctccctt
      121 ctccaagccc agccatgtat aactattacc aatcacacac ctgcgtatta ccaaatactc
      181 attaatgctt gcgcttgtgc ttgagcttac ccttacttcg tcaaccttgg atagcacacg
      241 gtacaaggcg tagcgcaacc cctgtggaga aagcacaccc acacacacac acacacccta
      301 tgtaccctat atattcctct gtaatccacc cctaacacgc tagtccctgg ccgctacgtt
      361 actacgactc agcacgctta ctcagctagt cagctactta ctagtcagct agctaggctc
      421 tacctgcatc acccatttag ctattcccgc tctgtctctt tgtaagtcat tttcagctcc
      481 ttttccgttt gcttcttcaa ttgccttcct ccattcttcg gctccccttc gatttctaat
      541 cggggactgg gcaaaaaaaa ttggaaaaag tatatttaaa ggttataaat ttgctcttct
      601 ttcctccaca ccctgagcac taggcagtac atttgcacgt gaaaaagggt aaattagctc
      661 aattcaatca atccaaaggt tagttacact agacccaccg caggaaggaa acataacaca
      721 tacaattaat taatccccta tgtgagacca gaccaataaa aaacgcccgg cggcaaccga
      781 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
      841 caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
      901 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
      961 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
     1021 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     1081 gctgaaacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
     1141 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
     1201 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
     1261 ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc
     1321 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
     1381 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
     1441 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
     1501 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
     1561 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg
     1621 atcaatcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1681 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1741 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1801 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1861 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1921 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1981 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2041 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2101 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2161 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2221 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2281 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2341 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta g
//
