LOCUS       sequence-467279-        7208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SFFV driven CCR6-ADAR2dd (as in XZ032).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: APL0043 SFFV-CCR6-ADAR2dd (FLP-IN)
FEATURES             Location/Qualifiers
     source          1..7208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(145..936,937..1005))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     768..787
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1006..1110)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1185..1204)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1392..1799
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             4177..4206
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4262..4279)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4268..4492
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4664..4683)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4815..4862
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4870..5892
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4870..5892
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6022..6143
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6059..6078)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6113..6132
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6192..6208)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6192..6208)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6205..6227)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6216..6232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6240..6246,6247..6264,6265..6270))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6285..6306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6423..6440)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6594..7182)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6674..6693)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
       61 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
      121 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
      181 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
      241 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
      301 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
      361 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
      421 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
      481 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
      541 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
      601 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
      661 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
      721 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
      781 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
      841 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
      901 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
      961 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     1021 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     1081 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     1141 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     1201 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     1261 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgccg ataaaataaa
     1321 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     1381 agctagctgc agtaacgcca ttttgcaagg catggaaaaa taccaaacca agaatagaga
     1441 agttcagatc aagggcgggt acatgaaaat agctaacgtt gggccaaaca ggatatctgc
     1501 ggtgagcagt ttcggccccg gcccggggcc aagaacagat ggtcaccgca gtttcggccc
     1561 cggcccgagg ccaagaacag atggtcccca gatatggccc aaccctcagc agtttcttaa
     1621 gacccatcag atgtttccag gctcccccaa ggacctgaaa tgaccctgcg ccttatttga
     1681 attaaccaat cagcctgctt ctcgcttctg ttcgcgcgct tctgcttccc gagctctata
     1741 aaagagctca caacccctca ctcggcgcgc cagtcctccg acagactgag tcgcccggga
     1801 agcttcgcca ccatgagcgg agagagcatg aacttcagtg atgtgttcga ttccagtgag
     1861 gactacttcg tgtccgtcaa tacatcctac tacagcgtag attctgagat gctgctttgc
     1921 tccctgcagg aggtgcgcca gttctcccga ctcttcgttc ccatcgcgta ttcactcatc
     1981 tgcgtctttg gcctcctggg gaatatcttg gtagtcatca catttgcttt ttataaaaag
     2041 gccagatcca tgacagacgt ctacctgctt aatatggcca ttgcagacat cctgttcgtc
     2101 ctgacgctcc cattctgggc tgtgagtcac gctaccgggg cctgggtgtt cagtaatgca
     2161 acctgcaagc tgttgaaggg aatttatgcc atcaacttta attgcggtat gctgctgctg
     2221 acttgcataa gtatggacag atacatcgcc atagtacagg caacaaaatc cttcagactg
     2281 aggtctcgaa cgctccctag gtctaaaatc atttgtcttg ttgtatgggg cttgagcgtg
     2341 attatttcca gcagtacttt cgtgtttaac cagaagtaca acacgcaggg cagcgatgtg
     2401 tgtgagccca agtaccagac ggtatctgag cctatcaggt ggaagctgtt gatgctgggt
     2461 ttggaattgc tgttcggctt ttttatccca ctcatgttta tgattttttg ttataccttc
     2521 atcgtgaaga ccctggtcca ggcacaaaac tctaagcgac acaaggccat ccgggtaatc
     2581 atcgctgttg tgctggtatt tctggcttgc cagatccctc ataatatggt ccttctggta
     2641 acggcagcca acctgggcaa aatgaatcgc tcatgtcagt cagaaaagct tatcgggtac
     2701 accaaaacgg ttacggaggt tctggcgttc ctgcattgtt gccttaaccc tgtgctgtac
     2761 gcatttatag gtcagaagtt tcgaaactac ttcctgaaga ttctcaaaga cctctggtgt
     2821 gtaagaagaa agtataaaag tagtggattt tcatgcgcag gacgctactc cgaaaacatt
     2881 tccagacaga ccagtgagac ggccgataac gataacgcct cctcctttac catgggtacc
     2941 ggtgctttgc acttggatca aacgccatct cgccagccta ttcccagtga gggtcttcag
     3001 ctgcatttac cgcaggtttt agctgacgct gtctcacgcc tggtcctggg taagtttggt
     3061 gacctgaccg acaacttctc ctcccctcac gctcgcagaa aagtgctggc tggagtcgtc
     3121 atgacaacag gcacagatgt taaagatgcc aaggtgataa gtgtttctac aggaacaaaa
     3181 tgtattaatg gtgaatacat gagtgatcgt ggccttgcat taaatgactg ccatgcagaa
     3241 ataatatctc ggagatcctt gctcagattt ctttatacac aacttgagct ttacttaaat
     3301 aacaaagatg atcaaaaaag atccatcttt cagaaatcag agcgaggggg gtttaggctg
     3361 aaggagaatg tccagtttca tctgtacatc agcacctctc cctgtggaga tgccagaatc
     3421 ttctcaccac atgagccaat cctggaagaa ccagcagata gacacccaaa tcgtaaagca
     3481 agaggacagc tacggaccaa aatagagtct ggtcagggga cgattccagt gcgctccaat
     3541 gcgagcatcc aagcgtggga cggggtgctg caaggggagc ggctgctcac catgtcctgc
     3601 agtgacaaga ttgcacgctg gaacgtggtg ggcatccagg gatccctgct cagcattttc
     3661 gtggagccca tttacttctc gagcatcatc ctgggcagcc tttaccacgg ggaccacctt
     3721 tccagggcca tgtaccagcg gatctccaac atagaggacc tgccacctct ctacaccctc
     3781 aacaagcctt tgctcagtgg catcagcaat gcagaagcac ggcagccagg gaaggccccc
     3841 aacttcagtg tcaactggac ggtaggcgac tccgctattg aggtcatcaa cgccacgact
     3901 gggaaggatg agctgggccg cgcgtcccgc ctgtgtaagc acgcgttgta ctgtcgctgg
     3961 atgcgtgtgc acggcaaggt tccctcccac ttactacgct ccaagattac caaacccaac
     4021 gtgtaccatg agtccaagct ggcggcaaag gagtaccagg ccgccaaggc gcgtctgttc
     4081 acagccttca tcaaggcggg gctgggggcc tgggtggaga agcccaccga gcaggaccag
     4141 ttctcactca cgccctgagc cgctcgagga gggcccgaac aaaaactcat ctcagaagag
     4201 gatctgaata gcgccgtcga ccatcatcat aatcatcatt gagtttaaac ccgctgatca
     4261 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     4321 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     4381 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     4441 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggagatctat
     4501 cgatgttctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     4561 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4621 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4681 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4741 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4801 ggttcacgta cctagaagtt cctattccga agttcctatt ctctagaaag tataggaact
     4861 tccttggcca aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     4921 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     4981 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     5041 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     5101 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     5161 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     5221 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     5281 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     5341 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     5401 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     5461 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     5521 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     5581 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     5641 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     5701 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     5761 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     5821 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     5881 gcaaaggaat agcacgtact acgagatttc gattccaccg ccgccttcta tgaaaggttg
     5941 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6001 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     6061 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6121 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     6181 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6241 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6301 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6361 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6421 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6481 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6541 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6601 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6661 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6721 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6781 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6841 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6901 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6961 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7021 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7081 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7141 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7201 tctacggg
//
