LOCUS       sequence-467289-        7373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SFFV driven AVPR2-ADAR2dd (as in XZ030).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 7373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: APL0031 SFFV-AVPR2-ADAR2dd (FLP-IN)
FEATURES             Location/Qualifiers
     source          1..7373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(145..936,937..1005))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     768..787
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1006..1110)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1185..1204)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1392..1799
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             4342..4371
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4427..4444)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4433..4657
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4829..4848)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4980..5027
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5035..6057
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5035..6057
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6187..6308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6224..6243)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6278..6297
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6357..6373)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6357..6373)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6370..6392)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6381..6397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6405..6411,6412..6429,6430..6435))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6450..6471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6588..6605)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6759..7347)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6839..6858)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
       61 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
      121 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
      181 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
      241 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
      301 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
      361 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
      421 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
      481 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
      541 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
      601 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
      661 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
      721 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
      781 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
      841 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
      901 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
      961 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     1021 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     1081 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     1141 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     1201 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     1261 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgccg ataaaataaa
     1321 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     1381 agctagctgc agtaacgcca ttttgcaagg catggaaaaa taccaaacca agaatagaga
     1441 agttcagatc aagggcgggt acatgaaaat agctaacgtt gggccaaaca ggatatctgc
     1501 ggtgagcagt ttcggccccg gcccggggcc aagaacagat ggtcaccgca gtttcggccc
     1561 cggcccgagg ccaagaacag atggtcccca gatatggccc aaccctcagc agtttcttaa
     1621 gacccatcag atgtttccag gctcccccaa ggacctgaaa tgaccctgcg ccttatttga
     1681 attaaccaat cagcctgctt ctcgcttctg ttcgcgcgct tctgcttccc gagctctata
     1741 aaagagctca caacccctca ctcggcgcgc cagtcctccg acagactgag tcgcccggga
     1801 agcttcgcca ccatgaagac gatcatcgcc ctgagctaca tcttctgcct ggtattcgcc
     1861 gactacaagg acgatgatga cgccagcatc gatatgctca tggcctctac cacaagcgcc
     1921 gtgcccggac atccatctct tccaagtctt ccctccaaca gctcacagga gaggcctctg
     1981 gatacccggg acccacttct tgcaagagcg gagctcgcct tgctctccat cgttttcgtt
     2041 gccgtcgcgt tgagcaatgg tctggtgctg gccgccctgg cgcgacgagg ccgccgaggc
     2101 cactgggcgc ctattcatgt ctttatcggc catctctgtc ttgcagacct ggccgtggcg
     2161 ctgtttcagg tgctccctca actggcttgg aaagccaccg atagattccg ggggccagac
     2221 gcactgtgtc gggccgtgaa atacctgcag atggtgggaa tgtatgccag ttcatatatg
     2281 atacttgcta tgaccttgga cagacacagg gctatctgtc gccccatgct ggcgtatagg
     2341 cacggttccg gagcccattg gaacaggcca gttctggttg cgtgggcttt ttccctgctt
     2401 ctgagcttgc cccagttgtt tattttcgca cagagaaatg tggagggcgg atctggcgtg
     2461 accgattgct gggcttgctt cgctgagcca tggggcagaa ggacctacgt gacgtggatt
     2521 gctctcatgg tgttcgttgc tcccaccctg gggatcgccg catgccaggt cctgatattc
     2581 agagaaatac acgcctcctt ggttcctggg ccaagcgagc gccctggtgg aagaagacgg
     2641 ggtcgaagga ctggctctcc aggcgaaggc gcccacgtat cagctgcggt agctaagacc
     2701 gtacgcatga cactggtcat cgtcgtcgtt tacgttcttt gctgggcccc gttttttttg
     2761 gtgcagctct gggcggcctg ggaccccgaa gcacccctgg aaggtgctcc attcgttctg
     2821 ctcatgcttc tcgcttcact gaatagctgt accaatcctt ggatttacgc ctctttctcc
     2881 tccagtgtta gctccgagct gcggagtctt ctctgttgcg cccggggcag gaccccccca
     2941 agcctgggtc cacaggacga atcctgcact accgcctcca gttccctggc caaagacacc
     3001 tcttctatcg ataccggtgg acgcacccca cccagcctgg gtccccaaga tgagtcctgc
     3061 accaccgcca gctcctccct ggccaaggac acttcatcgg gtaccggtgc tttgcacttg
     3121 gatcaaacgc catctcgcca gcctattccc agtgagggtc ttcagctgca tttaccgcag
     3181 gttttagctg acgctgtctc acgcctggtc ctgggtaagt ttggtgacct gaccgacaac
     3241 ttctcctccc ctcacgctcg cagaaaagtg ctggctggag tcgtcatgac aacaggcaca
     3301 gatgttaaag atgccaaggt gataagtgtt tctacaggaa caaaatgtat taatggtgaa
     3361 tacatgagtg atcgtggcct tgcattaaat gactgccatg cagaaataat atctcggaga
     3421 tccttgctca gatttcttta tacacaactt gagctttact taaataacaa agatgatcaa
     3481 aaaagatcca tctttcagaa atcagagcga ggggggttta ggctgaagga gaatgtccag
     3541 tttcatctgt acatcagcac ctctccctgt ggagatgcca gaatcttctc accacatgag
     3601 ccaatcctgg aagaaccagc agatagacac ccaaatcgta aagcaagagg acagctacgg
     3661 accaaaatag agtctggtca ggggacgatt ccagtgcgct ccaatgcgag catccaagcg
     3721 tgggacgggg tgctgcaagg ggagcggctg ctcaccatgt cctgcagtga caagattgca
     3781 cgctggaacg tggtgggcat ccagggatcc ctgctcagca ttttcgtgga gcccatttac
     3841 ttctcgagca tcatcctggg cagcctttac cacggggacc acctttccag ggccatgtac
     3901 cagcggatct ccaacataga ggacctgcca cctctctaca ccctcaacaa gcctttgctc
     3961 agtggcatca gcaatgcaga agcacggcag ccagggaagg cccccaactt cagtgtcaac
     4021 tggacggtag gcgactccgc tattgaggtc atcaacgcca cgactgggaa ggatgagctg
     4081 ggccgcgcgt cccgcctgtg taagcacgcg ttgtactgtc gctggatgcg tgtgcacggc
     4141 aaggttccct cccacttact acgctccaag attaccaaac ccaacgtgta ccatgagtcc
     4201 aagctggcgg caaaggagta ccaggccgcc aaggcgcgtc tgttcacagc cttcatcaag
     4261 gcggggctgg gggcctgggt ggagaagccc accgagcagg accagttctc actcacgccc
     4321 tgagccgctc gaggagggcc cgaacaaaaa ctcatctcag aagaggatct gaatagcgcc
     4381 gtcgaccatc atcataatca tcattgagtt taaacccgct gatcagcctc gactgtgcct
     4441 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4501 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4561 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4621 aatagcaggc atgctgggga tgcggtgggc tctatggaga tctatcgatg ttctggggat
     4681 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     4741 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4801 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4861 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4921 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag
     4981 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag
     5041 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     5101 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     5161 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     5221 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     5281 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     5341 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     5401 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     5461 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     5521 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     5581 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     5641 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     5701 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     5761 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     5821 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     5881 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     5941 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     6001 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac
     6061 gtactacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     6121 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     6181 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6241 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6301 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6361 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6421 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6481 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6541 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6601 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6661 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6721 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6781 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6841 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6901 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6961 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7021 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7081 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7141 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7201 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7261 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7321 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggg
//
