LOCUS       sequence-467293-       12302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LIDAR all components on single transcript (as shown in Figure 4A), 
            using EMCV IRES to separate each component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12302)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 12302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: XZ093 SFFV-sensor-IRESv0-LIDARst(IRESv0-AVPR2)
FEATURES             Location/Qualifiers
     source          1..12302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1211..1230)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1418..1825
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      1833..1842
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1839..2546
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1984..2002)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2252..2271
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2592..2657
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             2826..2891
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(2898..2900,2901..3611)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    join(3627..4201,4202..4204,4205..4213)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    join(6728..7302,7303..7305,7306..7314)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             9297..9326
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(9382..9399)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9388..9612
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9784..9803)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    9935..9982
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             9990..11012
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             9990..11012
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    11142..11263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11179..11198)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11233..11252
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11312..11328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11312..11328)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11325..11347)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11336..11352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11360..11366,11367..11384,11385..11390))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11405..11426
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11543..11560)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11714..12302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11794..11813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1201 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1261 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1321 tgaccgacaa ttgccgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     1381 gaaagacccc acctgtaggt ttggcaagct agctgcagta acgccatttt gcaaggcatg
     1441 gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg gcgggtacat gaaaatagct
     1501 aacgttgggc caaacaggat atctgcggtg agcagtttcg gccccggccc ggggccaaga
     1561 acagatggtc accgcagttt cggccccggc ccgaggccaa gaacagatgg tccccagata
     1621 tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc ccccaaggac
     1681 ctgaaatgac cctgcgcctt atttgaatta accaatcagc ctgcttctcg cttctgttcg
     1741 cgcgcttctg cttcccgagc tctataaaag agctcacaac ccctcactcg gcgcgccagt
     1801 cctccgacag actgagtcgc ccgggaagct tcgccaccat ggtgagcaag ggcgaggagg
     1861 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     1921 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     1981 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     2041 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatt cccgactact
     2101 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     2161 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     2221 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     2281 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     2341 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     2401 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     2461 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     2521 ccggcggcat ggacgagctg tacaagagcg acgcgttgga agccagacac aaacagaaaa
     2581 ttgtggctcc ggtgaaacag actttgaatt ttgaccttct gaagctggcg ggagacgtcg
     2641 agtccaaccc cggtcccggt accggatcct ccgtttaggt gggtggaata gtaacgtaca
     2701 ccatcagggt acgtctcaga caccatcagg gtctgtctgg tacagcatca gcgtaccata
     2761 gtatcccacc tacccagacg accggtacta gtgaggcacg gcataagcaa aagatcgtag
     2821 ccccagtaaa gcaaacactc aacttcgatc tactcaaact cgcaggtgat gtggaatcta
     2881 atccaggacc ctcgagtgtc tcaaagggcg aggaactttt tacgggcgtt gttccgatcc
     2941 tcgtcgaatt ggacggggac gttaatgggc ataaattctc agttagtggt gagggtgagg
     3001 gggacgcgac atatgggaaa cttacgctga agttcatatg taccaccgga aaattgccag
     3061 tgccgtggcc tactctcgtc accaccctga catatggtgt tcagtgtttt agccggtatc
     3121 cagaccatat gaaacagcac gattttttta aatccgctat gcctgaaggt tacgtacagg
     3181 agcgaacgat attttttaaa gacgacggaa actacaaaac tagagccgag gtaaaattcg
     3241 agggtgacac tctcgtgaac cggatcgaac tgaagggcat agattttaag gaggatggca
     3301 atatactggg ccacaagctc gaatacaact ataattctca taatgtctat atcatggcgg
     3361 acaaacagaa aaacggcatc aaggtaaact ttaaaatccg acacaatatt gaggacggct
     3421 ctgtccagct tgcggaccat taccaacaga acacgccgat aggggatggt ccagtacttc
     3481 tccctgataa tcactatttg tctactcagt cagctcttag caaggaccca aacgaaaaac
     3541 gggatcacat ggtccttctt gagttcgtca ctgcggctgg gataacactc gggatggacg
     3601 aactgtataa gtaggccgga gggccccccc tctccctccc ccccccctaa cgttactggc
     3661 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     3721 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     3781 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     3841 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     3901 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     3961 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     4021 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     4081 atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc gaggttaaaa
     4141 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     4201 tatggccaca accatgaaga cgatcatcgc cctgagctac atcttctgcc tggtattcgc
     4261 cgactacaag gacgatgatg acgccagcat cgatatgctc atggcctcta ccacaagcgc
     4321 cgtgcccgga catccatctc ttccaagtct tccctccaac agctcacagg agaggcctct
     4381 ggatacccgg gacccacttc ttgcaagagc ggagctcgcc ttgctctcca tcgttttcgt
     4441 tgccgtcgcg ttgagcaatg gtctggtgct ggccgccctg gcgcgacgag gccgccgagg
     4501 ccactgggcg cctattcatg tctttatcgg ccatctctgt cttgcagacc tggccgtggc
     4561 gctgtttcag gtgctccctc aactggcttg gaaagccacc gatagattcc gggggccaga
     4621 cgcactgtgt cgggccgtga aatacctgca gatggtggga atgtatgcca gttcatatat
     4681 gatacttgct atgaccttgg acagacacag ggctatctgt cgccccatgc tggcgtatag
     4741 gcacggttcc ggagcccatt ggaacaggcc agttctggtt gcgtgggctt tttccctgct
     4801 tctgagcttg ccccagttgt ttattttcgc acagagaaat gtggagggcg gatctggcgt
     4861 gaccgattgc tgggcttgct tcgctgagcc atggggcaga aggacctacg tgacgtggat
     4921 tgctctcatg gtgttcgttg ctcccaccct ggggatcgcc gcatgccagg tcctgatatt
     4981 cagagaaata cacgcctcct tggttcctgg gccaagcgag cgccctggtg gaagaagacg
     5041 gggtcgaagg actggctctc caggcgaagg cgcccacgta tcagctgcgg tagctaagac
     5101 cgtacgcatg acactggtca tcgtcgtcgt ttacgttctt tgctgggccc cgtttttttt
     5161 ggtgcagctc tgggcggcct gggaccccga agcacccctg gaaggtgctc cattcgttct
     5221 gctcatgctt ctcgcttcac tgaatagctg taccaatcct tggatttacg cctctttctc
     5281 ctccagtgtt agctccgagc tgcggagtct tctctgttgc gcccggggca ggaccccccc
     5341 aagcctgggt ccacaggacg aatcctgcac taccgcctcc agttccctgg ccaaagacac
     5401 ctcttctatc gataccggtg gacgcacccc acccagcctg ggtccccaag atgagtcctg
     5461 caccaccgcc agctcctccc tggccaagga cacttcatcg ggtaccggtg ctttgcactt
     5521 ggatcaaacg ccatctcgcc agcctattcc cagtgagggt cttcagctgc atttaccgca
     5581 ggttttagct gacgctgtct cacgcctggt cctgggtaag tttggtgacc tgaccgacaa
     5641 cttctcctcc cctcacgctc gcagaaaagt gctggctgga gtcgtcatga caacaggcac
     5701 agatgttaaa gatgccaagg tgataagtgt ttctacagga acaaaatgta ttaatggtga
     5761 atacatgagt gatcgtggcc ttgcattaaa tgactgccat gcagaaataa tatctcggag
     5821 atccttgctc agatttcttt atacacaact tgagctttac ttaaataaca aagatgatca
     5881 aaaaagatcc atctttcaga aatcagagcg aggggggttt aggctgaagg agaatgtcca
     5941 gtttcatctg tacatcagca cctctccctg tggagatgcc agaatcttct caccacatga
     6001 gccaatcctg gaagaaccag cagatagaca cccaaatcgt aaagcaagag gacagctacg
     6061 gaccaaaata gagtctggtc aggggacgat tccagtgcgc tccaatgcga gcatccaagc
     6121 gtgggacggg gtgctgcaag gggagcggct gctcaccatg tcctgcagtg acaagattgc
     6181 acgctggaac gtggtgggca tccagggatc cctgctcagc attttcgtgg agcccattta
     6241 cttctcgagc atcatcctgg gcagccttta ccacggggac cacctttcca gggccatgta
     6301 ccagcggatc tccaacatag aggacctgcc acctctctac accctcaaca agcctttgct
     6361 cagtggcatc agcaatgcag aagcacggca gccagggaag gcccccaact tcagtgtcaa
     6421 ctggacggta ggcgactccg ctattgaggt catcaacgcc acgactggga aggatgagct
     6481 gggccgcgcg tcccgcctgt gtaagcacgc gttgtactgt cgctggatgc gtgtgcacgg
     6541 caaggttccc tcccacttac tacgctccaa gattaccaaa cccaacgtgt accatgagtc
     6601 caagctggcg gcaaaggagt accaggccgc caaggcgcgt ctgttcacag ccttcatcaa
     6661 ggcggggctg ggggcctggg tggagaagcc caccgagcag gaccagttct cactcacgcc
     6721 ctgatagccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     6781 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     6841 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     6901 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     6961 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     7021 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     7081 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     7141 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     7201 ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     7261 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatgggg
     7321 gagaaacccg ggaccagggt cttcaagaag tcgagcccta actgcaagct caccgtgtac
     7381 ttgggcaagc gggacttcgt agatcacctg gacaaagtgg accctgtaga tggcgtggtg
     7441 cttgtggacc ctgactacct gaaggaccgc aaagtgtttg tgaccctcac ctgcgccttc
     7501 cgctatggcc gtgaagacct ggatgtgctg ggcttgtcct tccgcaaaga cctgttcatc
     7561 gccacctacc aggccttccc cccggtgccc aacccacccc ggccccccac ccgcctgcag
     7621 gaccggctgc tgaggaagct gggccagcat gcccacccct tcttcttcac cataccccag
     7681 aatcttccat gctccgtcac actgcagcca ggcccagagg atacaggaaa ggcctgcggc
     7741 gtagactttg agattcgagc cttctgtgct aaatcactag aagagaaaag ccacaaaagg
     7801 aactctgtgc ggctggtgat ccgaaaggtg cagttcgccc cggagaaacc cggcccccag
     7861 ccttcagccg aaaccacacg ccacttcctc atgtctgacc ggtccctgca cctcgaggct
     7921 tccctggaca aggagctgta ctaccatggg gagcccctca atgtaaatgt ccacgtcacc
     7981 aacaactcca ccaagaccgt caagaagatc aaagtctctg tgagacagta cgccgacatc
     8041 tgcctcttca gcaccgccca gtacaagtgt cctgtggctc aactcgaaca agatgaccag
     8101 gtatctccca gctccacatt ctgtaaggtg tacaccataa ccccactgct cagcgacaac
     8161 cgggagaagc ggggtctcgc cctggatggg aaactcaagc acgaggacac caacctggct
     8221 tccagcacca tcgtgaagga gggtgccaac aaggaggtgc tgggaatcct ggtgtcctac
     8281 agggtcaagg tgaagctggt ggtgtctcga ggcggggatg tctctgtgga gctgcctttt
     8341 gttcttatgc accccaagcc ccacgaccac atccccctcc ccagacccca gtcagccgct
     8401 ccggagacag atgtccctgt ggacaccaac ctcattgaat ttgataccaa ctatgccaca
     8461 gatgatgaca ttgtgtttga ggactttgcc cggcttcggc tgaaggggat gaaggatgac
     8521 gactatgatg atcaactctg cggcggtggt agcgctatgg cgagcaactt tacgcaattt
     8581 gtgttggttg acaatggtgg caccggagac gtaactgttg cgccgagcaa ttttgcgaac
     8641 gggattgcag aatggataag tagtaacagc cggagtcagg catataaagt cacatgctca
     8701 gttcgccaaa gttccgcaca gaaccgaaaa tataccataa aggtagaagt gccaaaaggg
     8761 gcgtggcgat cttatcttaa tatggagctt acgatcccaa tctttgcgac taattcagac
     8821 tgcgagctga ttgtcaaggc catgcaaggt cttttgaaag acggtaaccc gattccctct
     8881 gcgatcgccg ccaactcagg aatatatggt ggcggaaccg gtggcgggtc catggcctcc
     8941 aattttacac agtttgtgct cgtggacaac ggcggcactg gagacgtcac agtagctcca
     9001 agtaactttg cgaatgggat agccgagtgg atctcttcca attcaagatc acaagcctat
     9061 aaggtgactt gtagtgtacg ccaatctagt gcccagaacc ggaagtatac cattaaagtg
     9121 gaggtcccca aaggtgcatg gcgctcatac ttgaatatgg aattgacgat tccgatattt
     9181 gcgaccaata gcgactgcga gcttattgta aaagccatgc aaggacttct gaaagatggc
     9241 aacccgatac cttccgcaat cgctgcgaat agcggaattt actgattcga aggcccgaac
     9301 aaaaactcat ctcagaagag gatctgaata gcgccgtcga ccatcatcat aatcatcatt
     9361 gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     9421 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     9481 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     9541 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     9601 tgggctctat ggagatctat cgatgttctg gggatgcggt gggctctatg gcttctgagg
     9661 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     9721 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     9781 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     9841 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     9901 aaaaacttga ttagggtgat ggttcacgta cctagaagtt cctattccga agttcctatt
     9961 ctctagaaag tataggaact tccttggcca aaaagcctga actcaccgcg acgtctgtcg
    10021 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
    10081 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
    10141 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
    10201 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
    10261 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
    10321 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
    10381 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
    10441 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
    10501 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
    10561 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
    10621 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
    10681 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
    10741 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
    10801 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
    10861 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
    10921 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
    10981 gccccagcac tcgtccgagg gcaaaggaat agcacgtact acgagatttc gattccaccg
    11041 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
    11101 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
    11161 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    11221 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
    11281 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    11341 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    11401 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    11461 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    11521 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    11581 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    11641 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    11701 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    11761 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    11821 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    11881 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    11941 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    12001 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    12061 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    12121 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    12181 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    12241 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    12301 aa
//
