LOCUS       sequence-46730-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Wong SS, Kotera I, Mills E, Suzuki H, Truong K
  TITLE     Split-intein mediated re-assembly of genetically encoded Ca(2+) 
            indicators.
  JOURNAL   Cell Calcium. 2011 Nov 29.
  PUBMED    22133610
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Calcium. 2011 Nov 29."
COMMENT     Sequence Label: NpuDnaEc-cGCaMP2
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    30..54
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        70..179
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     primer_bind     complement(392..413)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(653..672)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cggacgaaat tatacgactc actantaggg gaattgtgag cggataacaa ttccccggag
       61 ttaatccggg acctttaatt caacccaaca caatatatta tagttaaata agaattatta
      121 tcaaatcatt tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatca
      181 aaggagatat accatgggcc tgactagtgg atcccatatg atcaaaatag ccacacgtaa
      241 atatttaggc aaacaaaatg tctatgacat tggagttgag cgcgaccata attttgcact
      301 caaaaatggc ttcatagctt ctaattgttt caatggtacc gctagcatgg tgagcaaggg
      361 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      421 ccacaagttc agcgtgtccg gcgagggtga gggcgatgcc acctacggca agctgaccct
      481 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      541 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      601 caagtccgcc atgcccgaag gctacatcca ggagcgcacc atcttcttca aggacgacgg
      661 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      721 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      781 cacgcgtgac caactgactg aagagcagat cgcagaattt aaagaggctt tctccctatt
//
