LOCUS       sequence-467310-        4723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with PhiYellow with 
            SpHis5 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4723)
  TITLE     Yeast tagging plasmids.
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-PhiYellow-SpHis5
FEATURES             Location/Qualifiers
     source          1..4723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..706
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="SSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQF
                     ICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNFK
                     TRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAFK
                     ICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKET
                     VRAVDCRKTYL"
     terminator      727..914
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(782..806)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            989..2189
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5 
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        989..1332
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1333..1986
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHTNQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMSNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     terminator      1992..2189
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2293..2312)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2294..2312)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2399..2416)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2570..3158)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2650..2669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3329..4120,4121..4189))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3952..3971
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4190..4294)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4362..4380
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4418..4440)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4540..4559
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4640..4658
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4640..4657
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 taactccagc ggcgccctgc tgttccacgg caagatcccc tacgtggtgg agatggaggg
       61 caatgtggat ggccacacct tcagcatccg cggcaagggc tacggcgatg ccagcgtggg
      121 caaggtggat gcccagttca tctgcaccac cggcgatgtg cccgtgccct ggagcaccct
      181 ggtgaccacc ctgacctacg gcgcccagtg cttcgccaag tacggccccg agctgaagga
      241 tttctacaag agctgcatgc ccgatggcta cgtgcaggag cgcaccatca ccttcgaggg
      301 cgatggcaat ttcaagaccc gcgccgaggt gaccttcgag aatggcagcg tgtacaatcg
      361 cgtgaagctg aatggccagg gcttcaagaa ggatggccac gtgctgggca agaatctgga
      421 gttcaatttc accccccact gcctgtacat ctggggcgat caggccaatc acggcctgaa
      481 gagcgccttc aagatctgcc acgagatcac cggcagcaag ggcgatttca tcgtggccga
      541 tcacacccag atgaataccc ccatcggcgg cggccccgtg cacgtgcccg agtaccacca
      601 catgagctac cacgtgaagc tgagcaagga tgtgaccgat caccgcgata atatgagcct
      661 gaaggagacc gtgcgcgccg tggattgccg caagacctac ctgtgaggcg cgccacttct
      721 aaataagcga atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata
      781 agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt
      841 aactctttcc tgtaggtcag gttgctttct caggtatagt atgaggtcgc tcttattgac
      901 cacacctcta ccggcagatc cgctagggat aacagggtaa tatagatctg tttagcttgc
      961 ctcgtccccg ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag
     1021 tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca
     1081 tccccatgta taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa
     1141 ttacggctcc tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa
     1201 ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt
     1261 cttctttcat atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga
     1321 acataaacaa ccatgggtag gagggctttt gtagaaagaa atacgaacga aacgaaaatc
     1381 agcgttgcca tcgctttgga caaagctccc ttacctgaag agtcgaattt tattgatgaa
     1441 cttataactt ccaagcatac aaaccaaaag ggagaacaag taatccaagt agacacggga
     1501 attggattct tggatcacat gtatcatgca ctggctaaac atgcaggctg gagcttacga
     1561 ctttactcaa gaggtgattt aatcatcgat gatcatcaca ctgcagaaga tactgctatt
     1621 gcacttggta ttgcattcaa gcaggctatg agtaactttg ccggcgttaa aagatttgga
     1681 catgcttatt gtccacttga cgaagctctt tctagaagcg tagttgactt gtcgggacgg
     1741 ccctatgctg ttatcgattt gggattaaag cgtgaaaagg ttggggaatt gtcctgtgaa
     1801 atgatccctc acttactata ttccttttcg gtagcagctg gaattacttt gcatgttacc
     1861 tgcttatatg gtagtaatga ccatcatcgt gctgaaagcg cttttaaatc tctggctgtt
     1921 gccatgcgcg cggctactag tcttactgga agttctgaag tcccaagcac gaagggagtg
     1981 ttgtaaagag tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt
     2041 ttttttatat tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc
     2101 gcctcgacat catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca
     2161 atcgtatgtg aatgctggtc gctatactgc tgtcgattcg atactaacgc cgccatccag
     2221 tttaaacgag ctcgaattca tcgatgatat cagatccact agtggcctat gcggccgcgg
     2281 atctgccggt ctccctatag tgagtcgtat taatttcgat aagccaggtt aacctgcatt
     2341 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     2401 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     2461 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2521 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2581 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2641 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2701 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2761 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2821 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2881 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2941 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3001 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3061 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3121 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3181 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3241 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3301 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     3361 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     3421 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     3481 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     3541 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3601 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3661 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3721 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3781 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3841 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3901 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3961 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     4021 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     4081 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     4141 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     4201 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     4261 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     4321 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     4381 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     4441 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     4501 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     4561 tgagagtgca ccatatggac atattgtcgt tagaacgcgg ctacaattaa tacataacct
     4621 tatgtatcat acacatacga tttaggtgac actatagaac gcggccgcca gctgaagctt
     4681 cgtacgctgc aggtcgacgg atcggtgacg gtgctggttt aat
//
