LOCUS       sequence-46739-e       13103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13103)
  AUTHORS   Henderson MJ, Russell AJ, Hird S, Munoz M, Clancy JL, Lehrbach GM, 
            Calanni ST, Jans DA, Sutherland RL, Watts CK
  TITLE     EDD, the human hyperplastic discs protein, has a role in 
            progesterone receptor coactivation and potential involvement in DNA 
            damage response.
  JOURNAL   J Biol Chem. 2002 Jul 19;277(29):26468-78. Epub 2002 May 13.
  PUBMED    12011095
REFERENCE   2  (bases 1 to 13103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-07-19"; volume: "277(29)"; pages: "26468-78. 
            Epub 2002 May 13"
COMMENT     Sequence Label: pEGFP-C1 EDD
FEATURES             Location/Qualifiers
     source          1..13103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             9730..9747
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    9891..10012
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9928..9947)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9982..10001
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(10019..10474)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10156..10177)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10368..10387
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10501..10605
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10607..10964
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10607..10798
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10630..10650)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10815..10950
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10999..11793
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10999..11793
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11053..11072)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11663..11682
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11981..12000)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    12025..12072
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      12401..12989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12890..12909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggaacgtc catccatttc gtggttcacc cgctgccggg caccgaggac
     1381 cagctcaatg acaggttacg agaagtttct gagaagctga acaaatataa tttaaacagc
     1441 cacccccctt tgaatgtatt ggaacaggct actattaaac agtgtgtggt gggaccaaat
     1501 catgctgcct ttcttcttga ggatggtaga gtttgcagga ttggtttttc agtacagcca
     1561 gacagattgg aattgggtaa acctgataat aatgatgggt caaagttgaa cagcaactcg
     1621 ggggcaggga ggacgtcaag gcctggtagg acaagcgact ctccatggtt tctctcaggt
     1681 tctgagactc taggcaggct ggcaggcaac accttaggaa gccgctggag ttctggagtg
     1741 ggtggaagtg gtggaggatc ctctggtagg tcatcagctg gagctcgaga ttcccgccgg
     1801 cagactcgag ttattcggac aggacgggat cgagggtctg ggcttttggg cagtcagccc
     1861 cagccagtta ttccagcatc tgtcattcca gaggagctga tttcacaggc ccaagttgtt
     1921 ttacaaggca aatccagaag tgtcattatt cgagaacttc agagaacaaa tcttgatgtg
     1981 aaccttgctg taaataattt acttagccgg gatgatgaag atggagatga tggggatgat
     2041 acagccagcg aatcttattt gcctggagag gatcttatgt ctctccttga tgccgacatt
     2101 cattctgccc acccaagtgt cattattgat gcagatgcca tgttttctga agacattagc
     2161 tattttggtt acccttcttt tcgtcgttca tcactttcca ggctaggctc atctcgagtt
     2221 ctccttcttc ccttagagag agactctgag ctgttgcgtg aacgtgaatc cgttttacgt
     2281 ttacgtgaac gaaggtggct tgatggagcc tcatttgata atgaaagggg ttctaccagc
     2341 aaggaaggag agccaaactt ggataagaag aatacacctg ttcaaagtcc agtatctcta
     2401 ggagaagatt tgcagtggtg gcctgataag gatggaacaa aattcatctg tattggggct
     2461 ctgtattctg aacttctggc tgtcagcagt aaaggagaac tttatcagtg gaaatggagt
     2521 gaatctgagc cttacagaaa tgcccagaat ccttcattac atcatccacg agcaacattt
     2581 ttggggttaa ccaatgaaaa gatagtcctc ctgtctgcaa atagcataag agcaactgta
     2641 gctacagaaa ataacaaggt tgctacatgg gtggatgaaa ctttaagttc tgtggcttct
     2701 aaattagagc acactgctca gacttactct gaacttcaag gagagcggat agtttcttta
     2761 cattgctgtg ccctttacac ctgcgctcag ctggaaaaca gtttatattg gtggggtgta
     2821 gttcctttta gtcaaaggaa gaaaatgtta gagaaagcta gagcaaaaaa taaaaagcct
     2881 aaatccagtg ctggtatttc ttcaatgccg aacatcactg ttggtaccca ggtatgcttg
     2941 agaaataatc ctctttatca tgctggagca gttgcatttt caattagtgc tgggattcct
     3001 aaagttggtg tcttaatgga gtcagtttgg aatatgaatg acagctgtag atttcaactt
     3061 agatctcctg aaagcttgaa aaacatggaa aaagctagca aaactactga agctaagcct
     3121 gaaagtaagc aggagccagt gaaaacagaa atgggtcctc caccatctcc agcatccacg
     3181 tgtagtgatg catcctcaat tgccagcagt gcatcaatgc catacaaacg acgacggtca
     3241 acccctgcac caaaagaaga ggaaaaggtg aatgaagagc agtggtctct tcgggaagtg
     3301 gtttttgtgg aagatgtcaa gaatgttcct gttggcaagg tgctaaaagt agatggtgcc
     3361 tatgttgctg taaaatttcc aggaacctcc agtaatacta actgtcagaa cagctctggt
     3421 ccagatgctg acccttcttc tctcctgcag gattgtaggt tacttagaat tgatgaattg
     3481 caggttgtca aaactggtgg aacaccgaag gttcccgact gtttccaaag gactcctaaa
     3541 aagctttgta tacctgaaaa aacagaaata ttagcagtga atgtagattc caaaggtgtt
     3601 catgctgttc tgaagactgg aaattgggtg cgatactgta tctttgatct tgctacagga
     3661 aaagcagaac aggaaaataa ttttcctaca agcagcattg ctttccttgg tcagaatgag
     3721 aggaatgtag ccattttcac tgctggacag gaatctccca ttattcttcg agatggaaat
     3781 ggtaccatct acccaatggc caaagattgc atgggaggaa taagggatcc cgattggctg
     3841 gatcttccac ctattagtag tcttggaatg ggtgtgcatt ctttaataaa tcttcctgcc
     3901 aattcaacaa tcaaaaagaa agctgctgtt atcatcatgg ctgtagagaa acaaacctta
     3961 atgcaacaca ttctgcgctg tgactatgag gcctgtcgac aatatctaat gaatcttgag
     4021 caagcggttg ttttagagca gaatctacag atgctgcaga cattcatcag ccacagatgt
     4081 gatggaaatc gaaatatttt gcatgcttgt gtatcagttt gctttccaac cagcaataaa
     4141 gaaactaaag aagaagagga agcggagcgt tctgaaagaa atacatttgc agaaaggctt
     4201 tctgctgttg aggccattgc aaatgcaata tcagttgttt caagtaatgg cccaggtaat
     4261 cgggctggat catcaagtag ccgaagtttg agattacggg aaatgatgag acgttcgttg
     4321 agagcagctg gtttgggtag acatgaagct ggagcttcat ccagtgacca ccaggatcca
     4381 gtttcacccc ccatagctcc ccctagttgg gttcctgacc ctcctgcgat ggatcctgat
     4441 ggtgacattg attttatcct ggcccccgct gtgggatctc ttaccacagc agcaaccggt
     4501 actggtcaag gaccaagcac ctccactatt ccaggtcctt ccacagagcc atctgtagta
     4561 gaatccaagg atcgaaaggc gaatgctcat tttatattga aattgttatg tgacagtgtg
     4621 gttctccagc cctatctacg agaacttctt tctgccaagg atgcaagagg gatgacccca
     4681 tttatgtcag ctgtaagtgg ccgagcttat cctgctgcaa ttaccatctt agaaactgct
     4741 cagaaaattg caaaagctga aatatcctca agtgaaaaag aggaagatgt attcatggga
     4801 atggtttgcc catcaggtac caaccctgat gactctcctt tatatgtttt atgttgtaat
     4861 gacacttgca gttttacatg gactggagca gagcacatta accaggatat ttttgagtgt
     4921 cgaacttgtg gcttgctgga gtcactgtgt tgttgtacgg aatgtgcaag ggtttgtcat
     4981 aaaggtcatg attgcaaact caaacggaca tcaccaacag cctactgtga ttgttgggag
     5041 aaatgtaaat gtaaaactct tattgctgga cagaaatctg ctcgtcttga tctactttat
     5101 cgcctgctca ctgctactaa tctggttact ctgccaaaca gcaggggaga gcacctctta
     5161 ctattcttag tacagacagt cgcaaggcag acggtggagc attgtcaata caggccacct
     5221 cgaatcaggg aagatcgtaa ccgaaaaaca gccagtcctg aagattcaga tatgccagat
     5281 catgatttag agcctccaag atttgcccag cttgcattgg agcgtgttct acaggactgg
     5341 aatgccttga aatctatgat tatgtttggg tcgcaggaga ataaagaccc tcttagtgcc
     5401 agcagtagaa taggccatct tttgccagaa gagcaagtat acctcaatca gcaaagtggc
     5461 acaattcggc tggactgttt cactcattgc cttatagtta agtgtacagc agatattttg
     5521 cttttagata ctctactagg tacactagtg aaagaactcc aaaacaaata tacacctgga
     5581 cgtagagaag aagctattgc tgtgacaatg aggtttctac gttcagtggc aagagttttt
     5641 gttattctga gtgtggaaat ggcttcatcc aaaaagaaaa acaactttat tccacagcca
     5701 attggaaaat gcaagcgtgt attccaagca ttgctacctt acgctgtgga agaattgtgc
     5761 aacgtagcag agtcactgat tgttcctgtc agaatgggga ttgctcgtcc aactgcacca
     5821 tttaccctgg ctagtactag catagatgcc atgcagggca gtgaagaatt attttcagtg
     5881 gaaccactac caccacgacc atcatctgat cagtctagca gctccagtca gtctcagtca
     5941 tcctacatca tcaggaatcc acagcagagg cgcatcagcc agtcacagcc cgttcggggc
     6001 agagatgaag aacaggatga tattgtttca gcagatgtgg aagaggttga ggtggtggag
     6061 ggtgtggctg gagaagagga tcatcatgat gaacaggaag aacacgggga agaaaatgct
     6121 gaggcagagg gacaacatga tgagcatgat gaagacggga gtgatatgga gctggacttg
     6181 ttagcagcag ctgaaacaga aagtgatagt gaaagtaacc acagcaacca agataatgct
     6241 agtgggcgca gaagcgttgt cactgcagca actgctggtt cagaagcagg agcaagcagt
     6301 gttcctgcct tcttttctga agatgattct caatcgaatg actcaagtga ttctgatagc
     6361 agtagtagtc agagtgacga catagaacag gagaccttta tgcttgatga gccattagaa
     6421 agaaccacaa atagctccca tgccaatggt gctgcccaag ctccccgttc aatgcagtgg
     6481 gctgtccgca acacccagca tcagcgagca gccagtacag ccccttccag tacatctaca
     6541 ccagcagcaa gttcagcggg tttgatttat attgatcctt caaacttacg ccggagtggt
     6601 accatcagta caagtgctgc agctgcagca gctgctttgg aagctagcaa cgccagcagt
     6661 tacctaacat ctgcaagcag tttagccagg gcttacagca ttgtcattag acaaatctcg
     6721 gacttgatgg gccttattcc taagtataat cacctagtat actctcagat tccagcagct
     6781 gtgaaattga cttaccaaga tgcagtaaac ttacagaact atgtagaaga aaagcttatt
     6841 cccacttgga actggatggt cagtattatg gattctactg aagctcaatt acgttatggt
     6901 tctgcattag catctgctgg tgatcctgga catccaaatc atcctcttca cgcttctcag
     6961 aattcagcga gaagagagag gatgactgcg cgagaagaag ctagcttacg aacacttgaa
     7021 ggcagacgac gtgccacctt gcttagcgcc cgtcaaggaa tgatgtctgc acgaggagac
     7081 ttcctaaatt atgctctgtc tctaatgcgg tctcataatg atgagcattc tgatgttctt
     7141 ccagttttgg atgtttgctc attgaagcat gtggcatatg tttttcaagc acttatatac
     7201 tggattaagg caatgaatca gcagacaaca ttggatacac ctcaactaga acgcaaaagg
     7261 acgcgagaac tcttggaact gggtattgat aatgaagatt cagaacatga aaatgatgat
     7321 gacaccaatc aaagtgctac tttgaatgat aaggatgatg actctcttcc tgcagaaact
     7381 ggccaaaacc atccattttt ccgacgttca gactccatga cattccttgg gtgtataccc
     7441 ccaaatccat ttgaagtgcc tctggctgaa gccatcccct tggctgatca gccacatctg
     7501 ttgcagccaa atgctagaaa ggaggatctt tttggccgtc caagtcaggg tctttattct
     7561 tcatctgcca gtagtgggaa atgtttaatg gaggttacag tggatagaaa ctgcctagag
     7621 gttcttccaa caaaaatgtc ttatgctgcc aatctgaaaa atgtaatgaa catgcaaaac
     7681 cggcaaaaaa aagaagggga agaacagccc gtgctgccag aagaaactga gagttcaaaa
     7741 ccagggccat ctgctcatga tcttgctgca caattaaaaa gtagcttact agcagaaata
     7801 ggacttactg aaagtgaagg gccacctctc acatctttca ggccacagtg tagctttatg
     7861 ggaatggtta tttcccatga tatgctgcta ggacgttggc gcctttcttt agaactgttc
     7921 ggcagggtat tcatggaaga tgttggagca gaacctggat caatcctaac tgaattgggt
     7981 ggttttgagg taaaagaatc aaaattccgc agagaaatgg aaaaactgag aaaccagcag
     8041 tcaagagatt tgtcactaga ggttgatcgg gatcgagatc ttctcattca gcagactatg
     8101 aggcagctta acaatcactt tggtcgaaga tgtgctacta caccaatggc tgtacacaga
     8161 gtaaaagtca catttaagga tgagccagga gagggcagtg gtgtagcacg aagtttttat
     8221 acagccattg cacaagcatt tttatcaaat gaaaaattgc caaatctaga gtgtatccaa
     8281 aatgccaaca aaggcaccca cacaagttta atgcagagat taaggaaccg aggagagaga
     8341 gaccgggaaa gggagagaga aagggaaatg aggaggagta gtggtttgcg agcaggttct
     8401 cggagggacc gggatagaga ctttagaaga cagctttcca tcgacactag gccctttaga
     8461 ccagcctctg aagggaatcc tagcgatgat cctgagcctt tgccagcaca tcggcaggca
     8521 cttggagaga ggctttatcc tcgtgtacaa gcaatgcaac cagcatttgc aagtaaaatc
     8581 actggcatgt tgttggaatt atccccagct cagctgcttc tccttctagc aagtgaggat
     8641 tctctgagag caagagtgga tgaggccatg gaactcatta ttgcacatgg acgggaaaat
     8701 ggagctgata gtatcctgga tcttggatta gtagactcct cagaaaaggt acagcaggaa
     8761 aaccgaaagc gccatggctc tagtcgaagt gtagtagata tggatttaga tgatacagat
     8821 gatggtgatg acaatgcccc tttgttttac caacctggga aaagaggatt ttatactcca
     8881 aggcctggca agaacacaga agcaaggttg aattgtttca gaaacattgg caggattctt
     8941 ggactatgtc tgttacagaa tgaactctgt cctatcacat tgaatagaca tgtaattaaa
     9001 gtattgcttg gtagaaaagt caattggcat gattttgctt tttttgatcc tgtaatgtat
     9061 gagagtttgc ggcaactaat cctcgcgtct cagagttcag atgctgatgc tgttttctca
     9121 gcaatggatt tggcatttgc aattgacctg tgtaaagaag aaggtggagg acaggttgaa
     9181 ctcattccta atggtgtaaa tataccagtc actccacaga atgtatatga gtatgtgcgg
     9241 aaatacgcag aacacagaat gttggtagtt gcagaacagc ccttacatgc aatgaggaaa
     9301 ggtctactag atgtgcttcc aaaaaattca ttagaagatt taacggcaga agattttagg
     9361 cttttggtaa atggctgcgg tgaagtcaat gtgcaaatgc tgatcagttt tacctctttc
     9421 aatgatgaat caggagaaaa tgctgagaag cttctgcagt tcaagcgttg gttctggtca
     9481 atagtagaga agatgagcat gacagaacga caagatcttg tttacttttg gacatcaagc
     9541 ccatcactgc cagccagtga agaaggattc cagcctatgc cctcaatcac aataagacca
     9601 ccagatgacc aacatcttcc tactgcaaat acttgcattt ctcgacttta cgtcccactc
     9661 tattcctcta aacagattct caaacagaaa ttgttactcg ccattaagac caagaatttt
     9721 ggttttgtgc atcaccatca ccatcactag ccgcgggccc gggatccacc ggatctagat
     9781 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     9841 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     9901 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9961 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa
    10021 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
    10081 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
    10141 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
    10201 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
    10261 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
    10321 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
    10381 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
    10441 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
    10501 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
    10561 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
    10621 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
    10681 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
    10741 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    10801 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    10861 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    10921 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
    10981 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
    11041 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
    11101 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
    11161 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
    11221 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
    11281 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
    11341 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
    11401 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
    11461 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
    11521 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
    11581 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
    11641 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
    11701 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
    11761 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
    11821 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
    11881 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
    11941 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
    12001 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
    12061 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
    12121 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
    12181 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
    12241 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    12301 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    12361 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    12421 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    12481 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    12541 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    12601 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    12661 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    12721 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    12781 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    12841 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    12901 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    12961 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    13021 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    13081 ataaccgtat taccgccatg cat
//
