LOCUS       sequence-46741-e       12652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12652)
  AUTHORS   Henderson MJ, Russell AJ, Hird S, Munoz M, Clancy JL, Lehrbach GM, 
            Calanni ST, Jans DA, Sutherland RL, Watts CK
  TITLE     EDD, the human hyperplastic discs protein, has a role in 
            progesterone receptor coactivation and potential involvement in DNA 
            damage response.
  JOURNAL   J Biol Chem. 2002 Jul 19;277(29):26468-78. Epub 2002 May 13.
  PUBMED    12011095
REFERENCE   2  (bases 1 to 12652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-07-19"; volume: "277(29)"; pages: "26468-78. 
            Epub 2002 May 13"
COMMENT     Sequence Label: pSG5 EDD C2768A
FEATURES             Location/Qualifiers
     source          1..12652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9..200
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(32..52)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      217..352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          395..967
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     975..994
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1022..1041
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1022..1040
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1051..1074
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9529..9546
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    9648..9769
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9685..9704)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9739..9758
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9803..9820)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9974..10562)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10054..10073)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10733..11524,11525..11593))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11356..11375
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11594..11698)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11766..11784
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11823..11845)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11945..11964
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(12031..12486)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12168..12189)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12380..12399
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12616..12638
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12630..12647
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12631..12647
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgacttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
       61 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      121 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      181 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      241 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      301 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      361 tgcaaaaagc tggatcgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
      421 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
      481 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
      541 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
      601 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
      661 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
      721 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
      781 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct
      841 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
      901 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
      961 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     1021 gtaatacgac tcactatagg gcgaattatg gactacaagg acgacgatga caagcctccg
     1081 tccgaagcgc gcggaattca aaggcgagtg gaggacgaga aggaaagcac catgacgtcc
     1141 atccatttcg tggttcaccc gctgccgggc accgaggacc agctcaatga caggttacga
     1201 gaagtttctg agaagctgaa caaatataat ttaaacagcc accccccttt gaatgtattg
     1261 gaacaggcta ctattaaaca gtgtgtggtg ggaccaaatc atgctgcctt tcttcttgag
     1321 gatggtagag tttgcaggat tggtttttca gtacagccag acagattgga attgggtaaa
     1381 cctgataata atgatgggtc aaagttgaac agcaactcgg gggcagggag gacgtcaagg
     1441 cctggtagga caagcgactc tccatggttt ctctcaggtt ctgagactct aggcaggctg
     1501 gcaggcaaca ccttaggaag ccgctggagt tctggagtgg gtggaagtgg tggaggatcc
     1561 tctggtaggt catcagctgg agctcgagat tcccgccggc agactcgagt tattcggaca
     1621 ggacgggatc gagggtctgg gcttttgggc agtcagcccc agccagttat tccagcatct
     1681 gtcattccag aggagctgat ttcacaggcc caagttgttt tacaaggcaa atccagaagt
     1741 gtcattattc gagaacttca gagaacaaat cttgatgtga accttgctgt aaataattta
     1801 cttagccggg atgatgaaga tggagatgat ggggatgata cagccagcga atcttatttg
     1861 cctggagagg atcttatgtc tctccttgat gccgacattc attctgccca cccaagtgtc
     1921 attattgatg cagatgccat gttttctgaa gacattagct attttggtta cccttctttt
     1981 cgtcgttcat cactttccag gctaggctca tctcgagttc tccttcttcc cttagagaga
     2041 gactctgagc tgttgcgtga acgtgaatcc gttttacgtt tacgtgaacg aaggtggctt
     2101 gatggagcct catttgataa tgaaaggggt tctaccagca aggaaggaga gccaaacttg
     2161 gataagaaga atacacctgt tcaaagtcca gtatctctag gagaagattt gcagtggtgg
     2221 cctgataagg atggaacaaa attcatctgt attggggctc tgtattctga acttctggct
     2281 gtcagcagta aaggagaact ttatcagtgg aaatggagtg aatctgagcc ttacagaaat
     2341 gcccagaatc cttcattaca tcatccacga gcaacatttt tggggttaac caatgaaaag
     2401 atagtcctcc tgtctgcaaa tagcataaga gcaactgtag ctacagaaaa taacaaggtt
     2461 gctacatggg tggatgaaac tttaagttct gtggcttcta aattagagca cactgctcag
     2521 acttactctg aacttcaagg agagcggata gtttctttac attgctgtgc cctttacacc
     2581 tgcgctcagc tggaaaacag tttatattgg tggggtgtag ttccttttag tcaaaggaag
     2641 aaaatgttag agaaagctag agcaaaaaat aaaaagccta aatccagtgc tggtatttct
     2701 tcaatgccga acatcactgt tggtacccag gtatgcttga gaaataatcc tctttatcat
     2761 gctggagcag ttgcattttc aattagtgct gggattccta aagttggtgt cttaatggag
     2821 tcagtttgga atatgaatga cagctgtaga tttcaactta gatctcctga aagcttgaaa
     2881 aacatggaaa aagctagcaa aactactgaa gctaagcctg aaagtaagca ggagccagtg
     2941 aaaacagaaa tgggtcctcc accatctcca gcatccacgt gtagtgatgc atcctcaatt
     3001 gccagcagtg catcaatgcc atacaaacga cgacggtcaa cccctgcacc aaaagaagag
     3061 gaaaaggtga atgaagagca gtggtctctt cgggaagtgg tttttgtgga agatgtcaag
     3121 aatgttcctg ttggcaaggt gctaaaagta gatggtgcct atgttgctgt aaaatttcca
     3181 ggaacctcca gtaatactaa ctgtcagaac agctctggtc cagatgctga cccttcttct
     3241 ctcctgcagg attgtaggtt acttagaatt gatgaattgc aggttgtcaa aactggtgga
     3301 acaccgaagg ttcccgactg tttccaaagg actcctaaaa agctttgtat acctgaaaaa
     3361 acagaaatat tagcagtgaa tgtagattcc aaaggtgttc atgctgttct gaagactgga
     3421 aattgggtgc gatactgtat ctttgatctt gctacaggaa aagcagaaca ggaaaataat
     3481 tttcctacaa gcagcattgc tttccttggt cagaatgaga ggaatgtagc cattttcact
     3541 gctggacagg aatctcccat tattcttcga gatggaaatg gtaccatcta cccaatggcc
     3601 aaagattgca tgggaggaat aagggatccc gattggctgg atcttccacc tattagtagt
     3661 cttggaatgg gtgtgcattc tttaataaat cttcctgcca attcaacaat caaaaagaaa
     3721 gctgctgtta tcatcatggc tgtagagaaa caaaccttaa tgcaacacat tctgcgctgt
     3781 gactatgagg cctgtcgaca atatctaatg aatcttgagc aagcggttgt tttagagcag
     3841 aatctacaga tgctgcagac attcatcagc cacagatgtg atggaaatcg aaatattttg
     3901 catgcttgtg tatcagtttg ctttccaacc agcaataaag aaactaaaga agaagaggaa
     3961 gcggagcgtt ctgaaagaaa tacatttgca gaaaggcttt ctgctgttga ggccattgca
     4021 aatgcaatat cagttgtttc aagtaatggc ccaggtaatc gggctggatc atcaagtagc
     4081 cgaagtttga gattacggga aatgatgaga cgttcgttga gagcagctgg tttgggtaga
     4141 catgaagctg gagcttcatc cagtgaccac caggatccag tttcaccccc catagctccc
     4201 cctagttggg ttcctgaccc tcctgcgatg gatcctgatg gtgacattga ttttatcctg
     4261 gcccccgctg tgggatctct taccacagca gcaaccggta ctggtcaagg accaagcacc
     4321 tccactattc caggtccttc cacagagcca tctgtagtag aatccaagga tcgaaaggcg
     4381 aatgctcatt ttatattgaa attgttatgt gacagtgtgg ttctccagcc ctatctacga
     4441 gaacttcttt ctgccaagga tgcaagaggg atgaccccat ttatgtcagc tgtaagtggc
     4501 cgagcttatc ctgctgcaat taccatctta gaaactgctc agaaaattgc aaaagctgaa
     4561 atatcctcaa gtgaaaaaga ggaagatgta ttcatgggaa tggtttgccc atcaggtacc
     4621 aaccctgatg actctccttt atatgtttta tgttgtaatg acacttgcag ttttacatgg
     4681 actggagcag agcacattaa ccaggatatt tttgagtgtc gaacttgtgg cttgctggag
     4741 tcactgtgtt gttgtacgga atgtgcaagg gtttgtcata aaggtcatga ttgcaaactc
     4801 aaacggacat caccaacagc ctactgtgat tgttgggaga aatgtaaatg taaaactctt
     4861 attgctggac agaaatctgc tcgtcttgat ctactttatc gcctgctcac tgctactaat
     4921 ctggttactc tgccaaacag caggggagag cacctcttac tattcttagt acagacagtc
     4981 gcaaggcaga cggtggagca ttgtcaatac aggccacctc gaatcaggga agatcgtaac
     5041 cgaaaaacag ccagtcctga agattcagat atgccagatc atgatttaga gcctccaaga
     5101 tttgcccagc ttgcattgga gcgtgttcta caggactgga atgccttgaa atctatgatt
     5161 atgtttgggt cgcaggagaa taaagaccct cttagtgcca gcagtagaat aggccatctt
     5221 ttgccagaag agcaagtata cctcaatcag caaagtggca caattcggct ggactgtttc
     5281 actcattgcc ttatagttaa gtgtacagca gatattttgc ttttagatac tctactaggt
     5341 acactagtga aagaactcca aaacaaatat acacctggac gtagagaaga agctattgct
     5401 gtgacaatga ggtttctacg ttcagtggca agagtttttg ttattctgag tgtggaaatg
     5461 gcttcatcca aaaagaaaaa caactttatt ccacagccaa ttggaaaatg caagcgtgta
     5521 ttccaagcat tgctacctta cgctgtggaa gaattgtgca acgtagcaga gtcactgatt
     5581 gttcctgtca gaatggggat tgctcgtcca actgcaccat ttaccctggc tagtactagc
     5641 atagatgcca tgcagggcag tgaagaatta ttttcagtgg aaccactacc accacgacca
     5701 tcatctgatc agtctagcag ctccagtcag tctcagtcat cctacatcat caggaatcca
     5761 cagcagaggc gcatcagcca gtcacagccc gttcggggca gagatgaaga acaggatgat
     5821 attgtttcag cagatgtgga agaggttgag gtggtggagg gtgtggctgg agaagaggat
     5881 catcatgatg aacaggaaga acacggggaa gaaaatgctg aggcagaggg acaacatgat
     5941 gagcatgatg aagacgggag tgatatggag ctggacttgt tagcagcagc tgaaacagaa
     6001 agtgatagtg aaagtaacca cagcaaccaa gataatgcta gtgggcgcag aagcgttgtc
     6061 actgcagcaa ctgctggttc agaagcagga gcaagcagtg ttcctgcctt cttttctgaa
     6121 gatgattctc aatcgaatga ctcaagtgat tctgatagca gtagtagtca gagtgacgac
     6181 atagaacagg agacctttat gcttgatgag ccattagaaa gaaccacaaa tagctcccat
     6241 gccaatggtg ctgcccaagc tccccgttca atgcagtggg ctgtccgcaa cacccagcat
     6301 cagcgagcag ccagtacagc cccttccagt acatctacac cagcagcaag ttcagcgggt
     6361 ttgatttata ttgatccttc aaacttacgc cggagtggta ccatcagtac aagtgctgca
     6421 gctgcagcag ctgctttgga agctagcaac gccagcagtt acctaacatc tgcaagcagt
     6481 ttagccaggg cttacagcat tgtcattaga caaatctcgg acttgatggg ccttattcct
     6541 aagtataatc acctagtata ctctcagatt ccagcagctg tgaaattgac ttaccaagat
     6601 gcagtaaact tacagaacta tgtagaagaa aagcttattc ccacttggaa ctggatggtc
     6661 agtattatgg attctactga agctcaatta cgttatggtt ctgcattagc atctgctggt
     6721 gatcctggac atccaaatca tcctcttcac gcttctcaga attcagcgag aagagagagg
     6781 atgactgcgc gagaagaagc tagcttacga acacttgaag gcagacgacg tgccaccttg
     6841 cttagcgccc gtcaaggaat gatgtctgca cgaggagact tcctaaatta tgctctgtct
     6901 ctaatgcggt ctcataatga tgagcattct gatgttcttc cagttttgga tgtttgctca
     6961 ttgaagcatg tggcatatgt ttttcaagca cttatatact ggattaaggc aatgaatcag
     7021 cagacaacat tggatacacc tcaactagaa cgcaaaagga cgcgagaact cttggaactg
     7081 ggtattgata atgaagattc agaacatgaa aatgatgatg acaccaatca aagtgctact
     7141 ttgaatgata aggatgatga ctctcttcct gcagaaactg gccaaaacca tccatttttc
     7201 cgacgttcag actccatgac attccttggg tgtatacccc caaatccatt tgaagtgcct
     7261 ctggctgaag ccatcccctt ggctgatcag ccacatctgt tgcagccaaa tgctagaaag
     7321 gaggatcttt ttggccgtcc aagtcagggt ctttattctt catctgccag tagtgggaaa
     7381 tgtttaatgg aggttacagt ggatagaaac tgcctagagg ttcttccaac aaaaatgtct
     7441 tatgctgcca atctgaaaaa tgtaatgaac atgcaaaacc ggcaaaaaaa agaaggggaa
     7501 gaacagcccg tgctgccaga agaaactgag agttcaaaac cagggccatc tgctcatgat
     7561 cttgctgcac aattaaaaag tagcttacta gcagaaatag gacttactga aagtgaaggg
     7621 ccacctctca catctttcag gccacagtgt agctttatgg gaatggttat ttcccatgat
     7681 atgctgctag gacgttggcg cctttcttta gaactgttcg gcagggtatt catggaagat
     7741 gttggagcag aacctggatc aatcctaact gaattgggtg gttttgaggt aaaagaatca
     7801 aaattccgca gagaaatgga aaaactgaga aaccagcagt caagagattt gtcactagag
     7861 gttgatcggg atcgagatct tctcattcag cagactatga ggcagcttaa caatcacttt
     7921 ggtcgaagat gtgctactac accaatggct gtacacagag taaaagtcac atttaaggat
     7981 gagccaggag agggcagtgg tgtagcacga agtttttata cagccattgc acaagcattt
     8041 ttatcaaatg aaaaattgcc aaatctagag tgtatccaaa atgccaacaa aggcacccac
     8101 acaagtttaa tgcagagatt aaggaaccga ggagagagag accgggaaag ggagagagaa
     8161 agggaaatga ggaggagtag tggtttgcga gcaggttctc ggagggaccg ggatagagac
     8221 tttagaagac agctttccat cgacactagg ccctttagac cagcctctga agggaatcct
     8281 agcgatgatc ctgagccttt gccagcacat cggcaggcac ttggagagag gctttatcct
     8341 cgtgtacaag caatgcaacc agcatttgca agtaaaatca ctggcatgtt gttggaatta
     8401 tccccagctc agctgcttct ccttctagca agtgaggatt ctctgagagc aagagtggat
     8461 gaggccatgg aactcattat tgcacatgga cgggaaaatg gagctgatag tatcctggat
     8521 cttggattag tagactcctc agaaaaggta cagcaggaaa accgaaagcg ccatggctct
     8581 agtcgaagtg tagtagatat ggatttagat gatacagatg atggtgatga caatgcccct
     8641 ttgttttacc aacctgggaa aagaggattt tatactccaa ggcctggcaa gaacacagaa
     8701 gcaaggttga attgtttcag aaacattggc aggattcttg gactatgtct gttacagaat
     8761 gaactctgtc ctatcacatt gaatagacat gtaattaaag tattgcttgg tagaaaagtc
     8821 aattggcatg attttgcttt ttttgatcct gtaatgtatg agagtttgcg gcaactaatc
     8881 ctcgcgtctc agagttcaga tgctgatgct gttttctcag caatggattt ggcatttgca
     8941 attgacctgt gtaaagaaga aggtggagga caggttgaac tcattcctaa tggtgtaaat
     9001 ataccagtca ctccacagaa tgtatatgag tatgtgcgga aatacgcaga acacagaatg
     9061 ttggtagttg cagaacagcc cttacatgca atgaggaaag gtctactaga tgtgcttcca
     9121 aaaaattcat tagaagattt aacggcagaa gattttaggc ttttggtaaa tggctgcggt
     9181 gaagtcaatg tgcaaatgct gatcagtttt acctctttca atgatgaatc aggagaaaat
     9241 gctgagaagc ttctgcagtt caagcgttgg ttctggtcaa tagtagagaa gatgagcatg
     9301 acagaacgac aagatcttgt ttacttttgg acatcaagcc catcactgcc agccagtgaa
     9361 gaaggattcc agcctatgcc ctcaatcaca ataagaccac cagatgacca acatcttcct
     9421 actgcaaata ctgccatttc tcgactttac gtcccactct attcctctaa acagattctc
     9481 aaacagaaat tgttactcgc cattaagacc aagaattttg gttttgtgca tcaccatcac
     9541 catcactagc ccctacgtcg acgagctcac tagtcgcggc cgctttcggg taccgagctc
     9601 tgaattcgat atcactagta attcggatcc agatcttatt aaagcagaac ttgtttattg
     9661 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9721 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggt
     9781 cgactctaga ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9841 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9901 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9961 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    10021 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    10081 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    10141 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
    10201 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10261 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10321 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10381 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    10441 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10501 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10561 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10621 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10681 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10741 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10801 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10861 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10921 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10981 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    11041 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    11101 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    11161 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    11221 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    11281 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    11341 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    11401 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    11461 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11521 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11581 gttgaatact catactcttc ttttttcaat attattgaag catttatcag ggttattgtc
    11641 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    11701 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
    11761 ataaaaatag gcgtatcacg aggccccttt cgtctcgcgc gtttcggtga tgacggtgaa
    11821 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
    11881 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
    11941 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
    12001 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
    12061 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
    12121 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
    12181 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
    12241 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
    12301 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
    12361 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
    12421 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
    12481 gcgcgtcgcg ccattcgcca ttcaggctac gcaactgttg ggaagggcga tcggtgcggg
    12541 cctcttcgct attacgccag ctggcgaagg ggggatgtgc tgcaaggcga ttaagttggg
    12601 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa tt
//
