LOCUS       sequence-467419-        9154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac donor plasmid, membrane-bound CCL20.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: XZ076 (PB) CMV-mCCL20 (PuroR-BFP)
FEATURES             Location/Qualifiers
     source          1..9154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1905..1967
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        2458..2837
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2838..3041
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2992..3012
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3043..3061
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3064..3082
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3092..3116
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3589..3678
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             4546..4575
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4631..4648)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4637..4844
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4877..4897)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4882..5211
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5062..5197
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5124..5143
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5260..5856
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5260..5279)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5756..5776
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5857..5913
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5920..6630
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(6693..6713)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6755..6876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6792..6811)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6846..6865
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7639..7673
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8164..8180)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8164..8180)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8177..8199)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8188..8204
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8212..8218,8219..8236,8237..8242))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8257..8278
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8395..8412)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8566..9154)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8646..8665)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
     1801 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
     1861 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
     1921 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc
     1981 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
     2041 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
     2101 caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc
     2161 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
     2221 gggctccgct ccggtccggc gctccccccg catcccagag ccggcagcgt gcggggacag
     2281 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
     2341 gagcctgcag acacctgggg ggatacgggg aaaaggcctc cacggccaca attgcatgaa
     2401 gaatctgctt agggttaggc gttttgcgct gcttcgtacg ggccagatat acgcgttgac
     2461 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     2521 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2581 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2641 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2701 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2761 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2821 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     2881 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2941 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     3001 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctccctatc agtgatagag
     3061 atctccctat cagtgataga gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct
     3121 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
     3181 ggactctagc gtttaaactt aagcttcgcc accatgtgct gtaccaagag tttgctcctg
     3241 gctgctttga tgtcagtgct gctactccac ctctgcggcg aatcagaagc agcaagcaac
     3301 tttgactgct gtcttggata cacagaccgt attcttcatc ctaaatttat tgtgggcttc
     3361 acacggcagc tggccaatga aggctgtgac atcaatgcta tcatctttca cacaaagaaa
     3421 aagttgtctg tgtgcgcaaa tccaaaacag acttgggtga aatatattgt gcgtctcctc
     3481 agtaaaaaag tcaagaacat gggtaccggg aacggaaatg gcaatggtaa cgggaacgga
     3541 aatggcaatg gtaacgggaa tactagtgtt aacaccggtg cggccgttta cccatacgat
     3601 gttcctgact atgcgggcta tccctatgac gtcccggact atgcaggatc ctatccatat
     3661 gacgttccag attacgctcc ggccgccacc ggtcttaagg tcgactttca gaaagctagc
     3721 tctattgttt ataagaagga gggggaacag gtggagttca gtttcccatt ggccttcaca
     3781 gtcgagaagc tcacaggatc aggagagttg tggtggcagg ccgaaagagc ctcctccagc
     3841 aagagttgga tcacttttga tctgaaaaac aaagaagtga gcgtgaagag agtcacacag
     3901 gaccctaagc tgcagatggg caagaagttg cctttgcact tgacactccc ccaagccctg
     3961 ccccagtatg ctggctccgg gaacctcacc ctcgccttgg aggctaagac cggaaagttg
     4021 catcaggagg tcaacctcgt ggtgatgagg gcaacacagc tgcagaagaa cctgacctgc
     4081 gaagtgtggg gtcccacctc acctaaactg atgctgtctc tgaagctgga gaataaggag
     4141 gctaaagtca gtaaaagaga gaaggctgtg tgggtcctta acccggaggc agggatgtgg
     4201 cagtgtcttc ttagcgactc tggacaggtg ctgctggaga gcaacatcaa ggtgcttcct
     4261 acttggtcaa caccagtaca gccgatggcc ctgatcgtac tcgggggagt cgccggcctg
     4321 cttctgttta tcggcctggg catcttcttc tgcgtacgct acaagtatcg caacagggat
     4381 gagggatctt accacgttga cgaatctagg aactatatca gtaacagcgc tcagagtaat
     4441 ggcgccgtgg ttaaagagaa acagccctcc agcgccaaat cagcaaacaa aaacaagaaa
     4501 aacaaggata aagagtacta cgtataggcc gctcgaggag ggcccgaaca aaaactcatc
     4561 tcagaagagg atctgaatag cgccgtcgac catcatcata atcatcattg agtttaaacc
     4621 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     4681 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     4741 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     4801 agcaaggggg aggattggga agacaatagc aggcatgctg gggacgcggc cgcaatttaa
     4861 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4921 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4981 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     5041 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5101 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     5161 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     5221 ggagcttgta tatccatttt cggatctgat cagcacgtga tgaccgagta caagcccacg
     5281 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     5341 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     5401 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     5461 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     5521 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     5581 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     5641 gtcggagtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     5701 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     5761 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     5821 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccgcca cgaacttctc tctgttaaag
     5881 caagcaggtg acgtggaaga aaaccccggt cccggatcca tggtgtctaa gggcgaagag
     5941 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac
     6001 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
     6061 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc
     6121 tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc
     6181 ttccctgagg gcttcacatg ggagagagtc accacatacg aggacggggg cgtgctgacc
     6241 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
     6301 gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc
     6361 accgaaacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag
     6421 ctcgtgggcg ggagccatct gatcgcaaac gccaagacca catatagatc caagaaaccc
     6481 gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag
     6541 gaggccaaca acgaaaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac
     6601 ctccctagca aactggggca caaacttaat taagtcgaca atcaacctct ggattacaaa
     6661 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6721 gctgctttaa tgcctttgta tcatgcgtta actaaacttg tttattgcag cttataatgg
     6781 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6841 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggaatt gactcaaatg
     6901 atgtcaatta gtctatcaga agctcatctg gtctcccttc cgggggacaa gacatccctg
     6961 tttaatattt aaacagcagt gttcccaaac tgggttctta tatcccttgc tctggtcaac
     7021 caggttgcag ggtttcctgt cctcacagga acgaagtccc taaagaaaca gtggcagcca
     7081 ggtttagccc cggaattgac tggattcctt ttttagggcc cattggtatg gctttttccc
     7141 cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga
     7201 tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg
     7261 ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag
     7321 ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctgatatc tataacaaga
     7381 aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt
     7441 atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt
     7501 cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc
     7561 aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag
     7621 agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaatct
     7681 agctgcatca ggatcatatc gtcgggtctt ttttccggct cagtcatcgc ccaagctggc
     7741 gctatctggg catcggggag gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat
     7801 ggaaagagtt tgccgaggat gactgctgct gcattgacgt tgagcgaaaa cgcacgttta
     7861 ccatgatgat tcgggaaggt gtggccatgc acgcctttaa cggtgaactg ttcgttcagg
     7921 ccacctggga taccagttcg tcgcggcttt tccggacaca gttccggatg gtcagcccga
     7981 agcgcatcag caacccgaac aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga
     8041 ttaatgacag cggtgcggcg ctgggatatt acgtcagcga ggacgggtat cctggctgga
     8101 tgccgcagaa atggacatgg ataccccgtg agttacccgg cgggcgcgct tggcgtaatc
     8161 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8221 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8281 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8341 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8401 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8461 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8521 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8581 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8641 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8701 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8761 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8821 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8881 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8941 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9001 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9061 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9121 gcagcagatt acgcgcagaa aaaaaggatc tcaa
//
