LOCUS       sequence-467424-       13719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of hCOL4A3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13719)
  TITLE     Jeff Miner Lab Plasmids
REFERENCE   2  (bases 1 to 13719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV-Hygro_hCOL4A3
FEATURES             Location/Qualifiers
     source          1..13719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1183
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1174
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1131..1151
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        6323..6822
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6358..6381)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6755..6774
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      6836..6845
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6857..7876
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             6857..7876
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    7890..8478
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7943..7963)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8361..8372)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8685..8918
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9047..9063)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9047..9063)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9060..9082)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9071..9087
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9095..9101,9102..9119,9120..9125))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9140..9161
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9278..9295)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9449..10037)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9529..9548)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10208..10999,11000..11068))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10831..10850
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11069..11173)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11221..11355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11258..11277)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11312..11331
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11523..12156
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12203..12328
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12825..13058
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13243..13287
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13550..13665
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacgc ccctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
      361 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
      421 ctggcttggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
      481 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
      541 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
      601 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
      661 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
      721 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
      781 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
      841 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
      901 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
      961 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     1021 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     1081 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     1141 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaacctgca ggatttaaat
     1201 tctagagcat gagcgcccgg accgccccca ggccgcaggt gctcctgctg ccgctcctgc
     1261 tggtgctcct ggcggcggcg cccgcagcca gcaagggttg tgtctgtaaa gacaaaggcc
     1321 agtgcttctg tgacggggcc aaaggggaga agggggagaa gggctttcct ggaccccccg
     1381 gttctcctgg ccagaaagga ttcacaggtc ctgaaggctt gcctggaccg cagggaccca
     1441 agggctttcc aggacttcca ggactcacgg gttccaaagg tgtaagggga ataagtggat
     1501 tgccaggatt ttctggttct cctggacttc caggcacccc aggcaatacc gggccttacg
     1561 gacttgtcgg tgtaccagga tgcagtggtt ctaagggtga gcaggggttt ccaggactcc
     1621 cagggacact gggctaccca gggatcccgg gtgctgctgg tttgaaagga caaaagggtg
     1681 ctcctgctaa agaagaagat atagaacttg atgcaaaagg cgaccccggg ttgccagggg
     1741 ctccaggacc ccagggtttg ccaggccctc caggttttcc tgggcctgtt ggcccacctg
     1801 gtcctccggg attctttggc tttccaggag ccatgggacc tagaggacct aagggtcaca
     1861 tgggtgaaag agtgatagga cataaaggag agcggggtgt gaaagggtta acaggacccc
     1921 cgggaccacc aggaacagtt attgtgaccc taactggccc agataacaga acggacctca
     1981 agggggaaaa gggagacaag ggagcaatgg gcgagcctgg acctcctgga ccctcaggac
     2041 tgcctggaga atcatatgga tctgaaaagg gtgctcctgg agaccctggc ctgcagggaa
     2101 aacccggaaa agatggtgtt cctggcttcc ctggaagtga gggagtcaag ggcaacaggg
     2161 gtttccctgg gttaatgggt gaagatggca ttaagggaca gaaaggggac attggccctc
     2221 caggatttcg tggtccaaca gaatattatg acacatacca ggaaaaggga gatgaaggca
     2281 ctccaggccc accagggccc agaggagctc gtggcccaca aggtcccagt ggtccccccg
     2341 gagttcctgg aagtcctgga tcatcaaggc ctggcctcag aggagcccct ggatggccag
     2401 gcctgaaagg aagtaaaggg gaacgaggcc gcccaggaaa ggatgccatg gggactcctg
     2461 ggtccccagg ttgtgctggt tcaccaggtc ttccaggatc accgggacct ccaggaccgc
     2521 caggtgacat cgtttttcgc aagggtccac ctggagatca cggactgcca ggctatctag
     2581 ggtctccagg aatcccagga gttgatgggc ccaaaggaga accaggcctc ctgtgtacac
     2641 agtgccctta tatcccaggg cctcccggtc tcccaggatt gccagggtta catggtgtaa
     2701 aaggaatccc aggaagacaa ggcgcagctg gcttgaaagg aagcccaggg tccccaggaa
     2761 atacaggtct tccaggattt ccaggtttcc caggtgccca gggtgaccca ggacttaaag
     2821 gagaaaaagg tgaaacactt cagcctgagg ggcaagtggg tgtcccaggt gacccggggc
     2881 tcagaggcca acctgggaga aagggcttgg atggaattcc tggaactccg ggagtgaaag
     2941 gattaccagg acctaaaggc gaactggctc tgagtggtga gaaaggggac caaggtcctc
     3001 caggggatcc tggctcccct gggtccccag gacctgcagg accagctgga ccacctggct
     3061 acggacccca aggagaacct ggtctccagg gcacgcaagg agttcctgga gcccccggac
     3121 cacccggaga agccggccct aggggagagc tcagtgtttc aacaccagtt ccaggcccac
     3181 caggacctcc agggccccct ggccatcctg gcccccaagg tccacctggt atccctggat
     3241 ccctggggaa atgtggagat cctggtcttc cagggcctga tggtgaacca ggaattccag
     3301 gaattggatt tcctgggcct cctggaccta agggagacca aggttttcca ggtacaaaag
     3361 gatcactggg ttgtcctgga aaaatgggag agcctgggtt acctggaaag ccaggcctcc
     3421 caggagccaa gggagaacca gcagtagcca tgcctggagg accaggaaca ccaggttttc
     3481 caggagaaag aggcaattct ggggaacatg gagaaattgg actccctgga cttccaggtc
     3541 tccctggaac tccaggaaat gaagggcttg atggaccacg aggagatcca gggcagcctg
     3601 gaccacctgg agaacaagga cccccaggaa ggtgcataga gggtcccagg ggagcccaag
     3661 gacttccagg cttaaatgga ttgaaagggc aacaaggcag aagaggtaaa acggggccaa
     3721 agggagaccc aggaattcca ggcttggata gatcaggatt tcctggagaa actggatcac
     3781 caggaattcc aggtcatcaa ggtgaaatgg gaccactggg tcaaagagga tatccaggaa
     3841 atccgggaat tttagggcca ccaggtgaag atggagtgat tgggatgatg ggctttcctg
     3901 gagccattgg ccctccaggg ccccctggga acccaggcac accagggcag agggggagcc
     3961 ctggaattcc aggagtaaag ggccagagag gaaccccagg agccaagggg gaacaaggag
     4021 ataaaggaaa tcccgggcct tcagagatat cccacgtaat aggggacaaa ggagaaccag
     4081 gtctcaaagg attcgcagga aatccaggtg agaaaggaaa cagaggcgtt ccagggatgc
     4141 caggtttaaa gggcctcaaa ggactacccg gaccagcagg accaccaggc cccagaggag
     4201 atttgggcag cactgggaat cctggagaac caggactgcg tggtatacca ggaagcatgg
     4261 ggaacatggg catgccaggt tctaaaggaa aaaggggaac tttgggattc ccaggtcgag
     4321 caggaagacc aggcctccca ggtattcatg gtctccaggg agataaggga gagccaggtt
     4381 attcagaagg tacaaggcca ggaccaccgg gaccaacggg ggatccagga ctgccgggtg
     4441 atatgggaaa gaaaggagaa atggggcaac ctggcccacc tggacatttg gggcctgctg
     4501 gacctgaggg agcccctgga agtcctggaa gtcctggcct cccaggaaag ccaggtcctc
     4561 atggtgattt gggttttaaa ggaatcaaag gcctcctggg ccctccagga atcagaggcc
     4621 ctccaggtct tccaggattt ccaggatctc ctggaccaat gggtataaga ggtgaccaag
     4681 gacgtgatgg aattcctggt ccagccggag aaaagggaga aacgggttta ttgagggccc
     4741 ctccaggccc aagagggaac cctggtgctc aaggagccaa aggagacagg ggagccccag
     4801 gttttcctgg cctcccgggc agaaaagggg ccatgggaga tgctggacct cgaggaccca
     4861 caggcataga aggattccca gggccaccag gtctgcccgg tgcaattatc cctggccaga
     4921 caggaaatcg tggtccacca ggctcaagag gaagcccagg tgcgcctggt ccccctggac
     4981 ctccagggag tcatgtaata ggcataaaag gagacaaagg gtctatgggc caccctggcc
     5041 caaaaggtcc acctggaact gcaggagaca tgggaccacc aggtcgtctg ggagcaccag
     5101 gtactccagg tcttccagga cccagaggtg atcctggatt ccaggggttt ccaggcgtga
     5161 aaggagaaaa gggtaatcct ggatttctag gatccattgg acctccagga ccaattgggc
     5221 caaaaggacc acctggtgta cgtggagacc ctggcacact taagattatc tcccttccag
     5281 gaagcccagg gccacctggc acacctggag aaccagggat gcagggagaa cctgggccac
     5341 cagggccacc tggaaaccta ggaccctgtg ggccaagagg taagccaggc aaggatggaa
     5401 aaccaggaac tcctggacca gctggagaaa aaggcaacaa aggttctaaa ggagagccag
     5461 gaccagctgg atcagatgga ttgccaggtt tgaaaggaaa acgtggagac agtggatcac
     5521 ctgcaacctg gacaacgaga ggctttgtct tcacccgaca cagtcaaacc acagcaattc
     5581 cttcatgtcc agaggggaca gtgccactct acagtgggtt ttcttttctt tttgtacaag
     5641 gaaatcaacg agcccacgga caagaccttg gaactcttgg cagctgcctg cagcgattta
     5701 ccacaatgcc attcttattc tgcaatgtca atgatgtatg taattttgca tctcgaaatg
     5761 attattcata ctggctgtca acaccagctc tgatgccaat gaacatggct cccattactg
     5821 gcagagccct tgagccttat ataagcagat gcactgtttg tgaaggtcct gcgatcgcca
     5881 tagccgttca cagccaaacc actgacattc ctccatgtcc tcacggctgg atttctctct
     5941 ggaaaggatt ttcattcatc atgttcacaa gtgcaggttc tgagggcacc gggcaagcac
     6001 tggcctcccc tggctcctgc ctggaagaat tccgagccag cccatttcta gaatgtcatg
     6061 gaagaggaac gtgcaactac tattcaaatt cctacagttt ctggctggct tcattaaacc
     6121 cagaaagaat gttcagaaag cctattccat caactgtgaa agctggggaa ttagaaaaaa
     6181 taataagtcg ctgtcaggtg tgcatgaaga aaagacactg atttgggccc aattcctgca
     6241 ggcgagctct cggctcgagc ggcggcggtg tgaccggcta ccggctgttc gaggagattc
     6301 tgtaaggccg cgcaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     6361 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     6421 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     6481 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     6541 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     6601 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     6661 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     6721 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     6781 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag ttaacgccgc
     6841 catggataga tccggaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     6901 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     6961 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     7021 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     7081 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     7141 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     7201 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     7261 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     7321 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     7381 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     7441 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     7501 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     7561 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     7621 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     7681 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     7741 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     7801 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     7861 gagggcaaag gaatagacgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa
     7921 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7981 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     8041 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     8101 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     8161 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     8221 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     8281 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     8341 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     8401 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     8461 tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc
     8521 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     8581 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     8641 ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca
     8701 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8761 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8821 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8881 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     8941 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg
     9001 ctagcgtttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     9061 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     9121 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     9181 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     9241 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9301 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9361 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9421 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9481 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9541 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9601 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9661 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9721 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9781 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9841 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9901 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9961 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    10021 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    10081 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    10141 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    10201 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    10261 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    10321 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    10381 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10441 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10501 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10561 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10621 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10681 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10741 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10801 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10861 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10921 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10981 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    11041 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    11101 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    11161 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
    11221 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    11281 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    11341 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    11401 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    11461 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    11521 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
    11581 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
    11641 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
    11701 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
    11761 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
    11821 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
    11881 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
    11941 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
    12001 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
    12061 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
    12121 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
    12181 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
    12241 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
    12301 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
    12361 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
    12421 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
    12481 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
    12541 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
    12601 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
    12661 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
    12721 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
    12781 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
    12841 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
    12901 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
    12961 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
    13021 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
    13081 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
    13141 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
    13201 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
    13261 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
    13321 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
    13381 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
    13441 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
    13501 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcct ttaaaagaaa
    13561 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
    13621 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
    13681 ggacagcaga gatccagttt atcgatatcg aattcgtga
//
