LOCUS       sequence-467426-       10910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express a RfxCas13d-compatible gRNA and to express HA Tagged 
            hfCas13d and a Puro resistance gene. It also encodes an mCherry 
            gene. The CasRx and Puro coding regions are separated by a T2A site.
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Back F, Sandoval A, Vu LM, Hong VM, Bhaskara A, Rodriguez SR, 
            O'Brien JT, Kolber BJ, Kroener S, Ploski JE
  TITLE     Adeno-associated viral vector resource for the RNA-targeting Cas13d:
            A comparison of high-fidelity variants, DjCas13d and hfCas13d.
  JOURNAL   Mol Ther Methods Clin Dev. 2025 Aug 20;33(4):101565. doi: 
            10.1016/j.omtm.2025.101565. eCollection 2025 Dec 11.
  PUBMED    41020266
REFERENCE   2  (bases 1 to 10910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2025-08-20"; volume: "33(4):101565. doi";
            pages: " 10.1016/j.omtm.2025.101565. eCollection 2025 Dec 11"
COMMENT     Sequence Label: pAK-DR30(SapI)-hfCas13d-Puro-pA/EF1A-mCherry-W...
FEATURES             Location/Qualifiers
     source          1..10910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        651..899
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     651..671
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     822..841
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        901..930
                     /label=RfxCas13d DR30
                     /note="5' processed 30-base direct repeat for an RfxCas13d 
                     gRNA (Konermann et al., 2018)"
     enhancer        1079..1364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     CDS             1902..1922
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1932..4829
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILVEYITNVVYVVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             4839..4859
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4869..4895
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4911..4964
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4965..5564
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4965..4984)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5461..5481
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5593..5610)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5599..5710
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5801..6979
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6032..6970
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6927..6947
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6992..7699
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(7134..7152)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7402..7421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7871..8459
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7924..7944)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8342..8353)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8690..8709)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   8823..8963
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   8823..8952
                     /label=AAV2 ITR
     rep_origin      9038..9493
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9125..9144)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9335..9356
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9510..9529)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9629..9651
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9689..9707)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9775..9879
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9880..9948,9949..10740)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10098..10117)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt gagggcctat
      661 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
      721 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
      781 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
      841 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
      901 aacccctacc aactggtcgg ggtttgaaac ggaagagcga gctcttcttt tttttggaca
      961 gcagagatcc agtttggtta attaatgcaa agatggataa agttttaaac agagaggaat
     1021 ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattctcta gaggtacccg
     1081 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     1141 cgtcaatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1201 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1261 tcaatgacgg taaatggccc gcctggcatt gtgcccagta catgacctta tgggactttc
     1321 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     1381 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     1441 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcccccgggg
     1501 gggggggggg gggggggggg gggggggggg gggggggggg ggggggggcc ggggggggcg
     1561 gggggggggg ccccccccag tttttttttt tttggggggg gggggggcgg cggccccccc
     1621 aacaacacgg cccccgcgcg ggggggggcg ccgcgcggcg cctccccccc gtgccccgct
     1681 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
     1741 gcgggcggga cggcccttct cctccgggct gtaattagct gagcaagagg taagggttta
     1801 agggatggtt ggttggtggg gtattaatgt ttaattacct ggagcacctg cctgaaatca
     1861 ctttttttca ggttggaccg gtcatcgatg ccaccatgag ccccaagaag aagagaaagg
     1921 tggaggccag catcgaaaaa aaaaagtcct tcgccaaggg catgggcgtg aagtccacac
     1981 tcgtgtccgg ctccaaagtg tacatgacaa ccttcgccga aggcagcgac gccaggctgg
     2041 aaaagatcgt ggagggcgac agcatcagga gcgtgaatga gggcgaggcc ttcagcgctg
     2101 aaatggccga taaaaacgcc ggctataaga tcggcaacgc caaattcagc catcctaagg
     2161 gctacgccgt ggtggctaac aaccctctgt atacaggacc cgtccagcag gatatgctcg
     2221 gcctgaagga aactctggaa aagaggtact tcggcgagag cgctgatggc aatgacaata
     2281 tttgtatcca ggtgatccat aacatcctgg acattgaaaa aatcctcgtg gaatacatta
     2341 ccaacgtggt gtacgtggtc aacaatatct ccggcctgga taaggacatt attggattcg
     2401 gcaagttctc cacagtgtat acctacgacg aattcaaaga ccccgagcac catagggccg
     2461 ctttcaacaa taacgataag ctcatcaacg ccatcaaggc ccagtatgac gagttcgaca
     2521 acttcctcga taaccccaga ctcggctatt tcggccaggc ctttttcagc aaggagggca
     2581 gaaattacat catcaattac ggcaacgaat gctatgacat tctggccctc ctgagcggac
     2641 tgaggcactg ggtggtccat aacaacgaag aagagtccag gatctccagg acctggctct
     2701 acaacctcga taagaacctc gacaacgaat acatctccac cctcaactac ctctacgaca
     2761 ggatcaccaa tgagctgacc aactccttct ccaagaactc cgccgccaac gtgaactata
     2821 ttgccgaaac tctgggaatc aaccctgccg aattcgccga acaatatttc agattcagca
     2881 ttatgaaaga gcagaaaaac ctcggattca atatcaccaa gctcagggaa gtgatgctgg
     2941 acaggaagga tatgtccgag atcaggaaaa atcataaggt gttcgactcc atcaggacca
     3001 aggtctacac catgatggac tttgtgattt ataggtatta catcgaagag gatgccaagg
     3061 tggctgccgc caataagtcc ctccccgata atgagaagtc cctgagcgag aaggatatct
     3121 ttgtgattaa cctgaggggc tccttcaacg acgaccagaa ggatgccctc tactacgatg
     3181 aagctaatag aatttggaga aagctcgaaa atatcatgca caacatcaag gaatttaggg
     3241 gaaacaagac aagagagtat aagaagaagg acgcccctag actgcccaga atcctgcccg
     3301 ctggccgtga tgtttccgcc ttcagcaaac tcatgtatgc cctgaccatg ttcctggatg
     3361 gcaaggagat caacgacctc ctgaccaccc tgattaataa attcgataac atccagagct
     3421 tcctgaaggt gatgcctctc atcggagtca acgctaagtt cgtggaggaa tacgcctttt
     3481 tcaaagactc cgccaagatc gccgatgagc tgaggctgat caagtccttc gctagaatgg
     3541 gagaacctat tgccgatgcc aggagggcca tgtatatcga cgccatccgt attttaggaa
     3601 ccaacctgtc ctatgatgag ctcaaggccc tcgccgacac cttttccctg gacgagaacg
     3661 gaaacaagct caagaaaggc aagcacggca tgagaaattt cattattaat aacgtgatca
     3721 gcaataaaag gttccactac ctgatcagat acggtgatcc tgcccacctc catgagatcg
     3781 ccaaaaacga ggccgtggtg aagttcgtgc tcggcaggat cgctgacatc cagaaaaaac
     3841 agggccagaa cggcaagaac cagatcgaca ggtactacga aacttgtatc ggaaaggata
     3901 agggcaagag cgtgagcgaa aaggtggacg ctctcacaaa gatcatcacc ggaatgaact
     3961 acgaccaatt cgacaagaaa aggagcgtca ttgaggacac cggcagggaa aacgccgaga
     4021 gggagaagtt taaaaagatc atcagcctgt acctcaccgt gatctaccac atcctcaaga
     4081 atattgtcaa tatcaacgcc aggtacgtca tcggattcca ttgcgtcgag cgtgatgctc
     4141 aactgtacaa ggagaaaggc tacgacatca atctcaagaa actggaagag aagggattca
     4201 gctccgtcac caagctctgc gctggcattg atgaaactgc ccccgataag agaaaggacg
     4261 tggaaaagga gatggctgaa agagccaagg agagcattga cagcctcgag agcgccaacc
     4321 ccaagctgta tgccaattac atcaaataca gcgacgagaa gaaagccgag gagttcacca
     4381 ggcagattaa cagggagaag gccaaaaccg ccctgaacgc ctacctgagg aacaccaagt
     4441 ggaatgtgat catcagggag gacctcctga gaattgacaa caagacatgt accctgttca
     4501 gaaacaaggc cgtccacctg gaagtggcca ggtatgtcca cgcctatatc aacgacattg
     4561 ccgaggtcaa ttcctacttc caactgtacc attacatcat gcagagaatt atcatgaatg
     4621 agaggtacga gaaaagcagc ggaaaggtgt ccgagtactt cgacgctgtg aatgacgaga
     4681 agaagtacaa cgataggctc ctgaaactgc tgtgtgtgcc tttcggctac tgtatcccca
     4741 ggtttaagaa cctgagcatc gaggccctgt tcgataggaa cgaggccgcc aagttcgaca
     4801 aggagaaaaa gaaggtgtcc ggcaattccg gatccggacc taagaaaaag aggaaggtgg
     4861 cggccgctta cccatacgat gttccagatt acgctgctag cggcagtgga gagggcagag
     4921 gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaatgacc gagtacaagc
     4981 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     5041 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     5101 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     5161 tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     5221 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     5281 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     5341 ccaccgtcgg agtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     5401 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     5461 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     5521 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgtacg ctaactagag
     5581 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     5641 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     5701 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     5761 acagcaaggg ggaggattgg gaagagaata gcaggcatgc ggctccggtg cccgtcagtg
     5821 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     5881 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     5941 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     6001 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     6061 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     6121 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     6181 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     6241 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     6301 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     6361 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     6421 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     6481 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     6541 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     6601 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     6661 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     6721 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     6781 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     6841 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     6901 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     6961 tccatttcag gtgtcgtgag gtaccggatc cgtgagcaag ggcgaggagg ataacatggc
     7021 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     7081 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     7141 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     7201 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     7261 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     7321 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     7381 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     7441 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     7501 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     7561 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     7621 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     7681 ggacgagctg tacaagtaag gcgcgccccc ccctaacgtt actggccgaa gccgcttgga
     7741 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     7801 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaaat tcgatatcaa
     7861 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7921 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7981 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     8041 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     8101 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     8161 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     8221 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     8281 ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     8341 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     8401 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     8461 tcgataccga gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg
     8521 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta
     8581 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg
     8641 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa
     8701 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa
     8761 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgctg gggagcggcc
     8821 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     8881 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     8941 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     9001 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     9061 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
     9121 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     9181 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     9241 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     9301 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     9361 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
     9421 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     9481 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     9541 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     9601 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     9661 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     9721 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     9781 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9841 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9901 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9961 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
    10021 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
    10081 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
    10141 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    10201 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    10261 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    10321 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    10381 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    10441 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    10501 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    10561 tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat tgcagcactg
    10621 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    10681 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10741 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10801 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10861 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
