LOCUS       sequence-467430-       11891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS 
            expressing plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11891)
  AUTHORS   Fu Y, Land M, Cui R, Kavlashvili T, Kim M, Lieber T, Ryu KW, 
            DeBitetto E, Masilionis I, Saha R, Takizawa M, Baker D, Tigano M, 
            Reznik E, Sharma R, Chaligne R, Thompson CB, Pe'er D, Sfeir A
  TITLE     Engineering mtDNA Deletions by Reconstituting End-Joining in Human 
            Mitochondria.
  JOURNAL   bioRxiv [Preprint]. 2024 Oct 17:2024.10.15.618543. doi: 
            10.1101/2024.10.15.618543.
  PUBMED    39463974
REFERENCE   2  (bases 1 to 11891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Oct 17:2024.10.15.618543. doi: 
            10.1101/2024.10.15.618543."
COMMENT     Sequence Label: pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato
FEATURES             Location/Qualifiers
     source          1..11891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     117..136
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             310..1740
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    1754..2342
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1807..1827)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2225..2236)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2490..2670
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2717..2735)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2803..2907
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2908..2976,2977..3768)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3126..3145)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3939..4527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4681..4698
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4938..4959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4974..4979,4980..4997,4998..5004)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5012..5028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5017..5039
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5036..5052
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5036..5052
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5102..5121
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5313..5946
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5993..6118
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6615..6848
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7033..7077
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7370..7487
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7535..8713
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7766..8704
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8661..8681
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             9000..9047
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     misc_feature    join(11620..11891,1..296)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    join(11637..11891,1..298)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(11781..11798)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
       61 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
      121 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
      181 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
      241 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
      301 gccacacata tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg
      361 cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      421 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
      481 gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc
      541 gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
      601 atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc
      661 acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg
      721 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg
      781 ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag
      841 ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac
      901 accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc
      961 tccgagggcc gccaccacct gttcctgggg catggcaccg gcagcaccgg cagcggcagc
     1021 tccggcaccg cctcctccga ggacaacaac atggccgtca tcaaagagtt catgcgcttc
     1081 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     1141 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
     1201 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag
     1261 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag
     1321 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag
     1381 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc
     1441 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
     1501 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg
     1561 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac
     1621 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     1681 gagcgctccg agggccgcca ccacctgttc ctgtacggca tggacgagct gtacaagtaa
     1741 atcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     1801 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     1861 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     1921 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     1981 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     2041 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     2101 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     2161 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     2221 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     2281 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     2341 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     2401 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat cacctgcagg
     2461 acaggcgcgc cctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     2521 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     2581 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     2641 ccttttagtc agtgtggaaa atctctagca cccgggcgat taaggaaagg gctagatcat
     2701 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     2761 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2821 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2881 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     2941 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     3001 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3061 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3121 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     3181 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3241 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3301 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3361 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3421 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3481 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3541 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3601 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3661 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3721 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     3781 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3841 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3901 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3961 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4021 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4081 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4141 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4201 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4261 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4321 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4381 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4441 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4501 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4561 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4621 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4681 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     4741 cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag
     4801 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4861 gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc
     4921 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     4981 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     5041 aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt
     5101 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
     5161 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
     5221 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
     5281 atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag
     5341 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
     5401 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     5461 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     5521 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
     5581 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
     5641 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
     5701 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     5761 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5821 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5881 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5941 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     6001 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     6061 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     6121 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     6181 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     6241 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     6301 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     6361 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     6421 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct
     6481 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     6541 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     6601 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     6661 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     6721 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     6781 tggggcatca agcagcttca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     6841 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     6901 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     6961 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     7021 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     7081 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     7141 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     7201 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     7261 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     7321 ggatctcgac ggtatcgccg aattcacaaa tggcagtatt catccacaat tttaaaagaa
     7381 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     7441 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     7501 gggacagcag agatccagtt tggactagtc gtgaggctcc ggtgcccgtc agtgggcaga
     7561 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
     7621 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     7681 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     7741 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
     7801 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
     7861 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
     7921 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
     7981 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
     8041 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
     8101 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
     8161 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     8221 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     8281 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     8341 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     8401 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     8461 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     8521 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     8581 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     8641 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     8701 tcaggtgtcg tgaagcggcc gcttaattaa gtttctcgcg gtgcccttgc ggcgcagctg
     8761 gggtcgcggc cctgctcccc gcgctttctt aaggcccgcg ggcggcgcag gagcggcact
     8821 cgtggctgtg gtggcttcgg cagcggcttc agcagatcgg cggcatcagc ggtagcacca
     8881 gcactagcag catgagaaaa atgctggctg ccgtgagccg agtgctgtcc ggggcaagtc
     8941 aaaagccagc ctcaagagtc ttggtggcaa gtagaaattt cattcatggt accgtcgacg
     9001 actacaagga cgacgatgac aaggactaca aagacgatga cgacaaggga tccggttcag
     9061 catctgagca acgggtcact cttacaaacg ctgataaagt tttgtaccca gcaactggaa
     9121 ccacaaaatc tgacattttt gattattacg caggcgtcgc ggaggtcatg ctgggacaca
     9181 tagcaggcag gcctgcgacc cggaaacgat ggccgaacgg ggttgaccaa ccggcctttt
     9241 ttgaaaagca gcttgctctc agtgctcctc catggctgtc ccgggctacc gttgcccatc
     9301 gctccggtac gacgacgtat ccaatcatag attctgcgac tggtcttgct tggattgcgc
     9361 agcaggccgc actggaagtt catgtgcccc agtggcgctt tgtcgctgaa cctggcagtg
     9421 gggaactcaa tccaggccca gcaactcggc ttgtattcga cctggacccc ggagaaggcg
     9481 tcatgatggc tcaattggcg gaggtcgcta gggccgtgcg ggacttgttg gctgatatcg
     9541 gacttgtcac tttccctgta acatccggtt ccaaggggct gcatctgtac acccccctgg
     9601 acgagcctgt ctccagtcga ggagcgactg ttcttgctaa gcgagtcgct cagaggctgg
     9661 aacaagccat gcccgcactc gtaacgagca ctatgacaaa aagtctgagg gccggcaagg
     9721 ttttcgtcga ttggtctcag aactccggca gtaaaacgac gatcgcaccc tacagcttga
     9781 ggggtcggac gcatcccaca gtcgctgcgc ccaggacgtg ggcagagctg gacgacccag
     9841 cactgcgcca actctcatat gacgaagtcc ttacacgaat tgcgcgcgat ggggatcttc
     9901 ttgaacgcct cgacgcagat gcgcctgtgg ctgatcgctt gaccagatac cgccggatgc
     9961 gcgacgcaag caaaactcct gagcccatac ctactgctaa gccagtaact ggcgatggga
    10021 atacttttgt tatacaagaa caccacgcac ggagacccca ttacgatttc cgcttggagt
    10081 gcgacggggt tcttgtatct tgggcggtgc caaaaaactt gcctgacaac acctccgtga
    10141 accatctggc aattcacaca gaagaccatc cgcttgaata cgctacattt gagggtgcga
    10201 ttccttctgg cgaatatggc gctggcaagg ttataatttg ggactctgga acctacgata
    10261 ctgaaaagtt tcacgatgac ccacacaccg gcgaagtcat cgttaatctc catgggggta
    10321 gaatctctgg cagatatgct ctgatccgaa cgaatggaga caggtggctg gcacataggc
    10381 tcaagaacca aaaagaccag aaggtatttg agtttgataa cttggcaccc atgcttgcta
    10441 cacacgggac agtcgctgga ctcaaagcct cacaatgggc ttttgaaggt aaatgggatg
    10501 gataccggtt gcttgtcgag gcggaccatg gagcggttag gcttaggtca cgctcagggc
    10561 gcgacgtgac tgcggagtat ccccaacttc gggcactggc ggaagatttg gccgaccacc
    10621 acgtcgtact ggatggggaa gcagtcgtcc tggacagttc tggagtcccc tcattttccc
    10681 aaatgcagaa taggggtcga gatacacgag ttgagttctg ggctttcgac ctgctgtacc
    10741 tcgatgggcg ggcgctcttg ggaactcgct atcaagacag aaggaaactc ctggaaacgc
    10801 ttgccaacgc aacttctctt acagtgccgg agcttttgcc aggtgatggg gctcaagcct
    10861 tcgcttgctc aagaaaacac ggatgggagg gtgtgattgc caagaggcgc gacagtagat
    10921 accagcctgg gcgcagatgc gcgagctggg tcaaagacaa acattggaac acacaagaag
    10981 ttgttatagg gggctggagg gctggagaag gtggacggtc ttcaggtgtg ggaagccttc
    11041 tcatgggcat acccggtcca ggtgggttgc agttcgcagg ccgcgtagga actgggttgt
    11101 cagaaaggga attggcgaac cttaaggaaa tgttggctcc actccacact gacgaaagcc
    11161 ctttcgatgt tcctttgccg gctcgagatg caaaaggtat tacctatgta aaacccgccc
    11221 tcgttgcgga agtccgctac agtgaatgga cccccgaggg aaggctcaga caaagcagtt
    11281 ggagaggcct gagaccggat aaaaagccgt ccgaagtggt ccgcgaagct agcgctctgc
    11341 agaaaaaact cgaggagctg gaactggatt ctagataaac cacgatcgtt atgctgagta
    11401 tgttaagctc tttatgactg tttttgtagt ggtatagagt actgcagaat acagtaagct
    11461 gctctattgt agcatttctt gatgttgctt agtcacttat ttcataaaca acttaatgtt
    11521 ctgaataatt tcttactaaa cattttgtta ttgggcaagt gattgaaaat agtaaatgct
    11581 ttgtgtgatt gaatcgaatt cagggcccgt ttaaacggat ccctcccccc cccctaacgt
    11641 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
    11701 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
    11761 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
    11821 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
    11881 gcagcggaac c
//
