LOCUS       sequence-467437-       10275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac donor plasmid to overexpress CCR6-ADAR2dd (gLIDAR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10275)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 10275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: LSM0250 (PB)EF1a-CCR6V2-ADAR2dd-IRESv0-PuroR-P...
FEATURES             Location/Qualifiers
     source          1..10275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1905..1967
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2550..3728
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2781..3719
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3676..3696
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    join(6260..6834,6835..6837,6838..6846)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6427..6444)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6654..6673
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6847..7443
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6847..6866)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7343..7363
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7444..7500
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7507..8217
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(8251..8268)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8257..8464
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8760..8794
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9285..9301)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9285..9301)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9298..9320)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9309..9325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9333..9339,9340..9357,9358..9363))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9378..9399
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9516..9533)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9687..10275)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9767..9786)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
     1801 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
     1861 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
     1921 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc
     1981 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
     2041 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
     2101 caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc
     2161 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
     2221 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
     2281 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
     2341 gagcctgcag acacctgggg ggatacgggg aaaaggcctc cacggccact agtttcgcac
     2401 gattgcacct tgggagcctt aaccgctttt gaatgtccgt cttagttaat ccctaattga
     2461 acgctccaaa aggtgacttc caatagggat ttattccttt taaaattaac ggcataatcg
     2521 tgccgccgaa tgcaacctcg atcgagccgg gctccggtgc ccgtcagtgg gcagagcgca
     2581 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2641 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2701 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2761 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     2821 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     2881 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     2941 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     3001 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     3061 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     3121 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     3181 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     3241 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     3301 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     3361 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     3421 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     3481 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     3541 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     3601 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     3661 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     3721 tgtcgtgaat ctagaagcca ccatgaagac gatcatcgcc ctgagctaca tcttctgcct
     3781 ggtattcgcc gactacaagg acgatgatga cgccagcatc gatatgagcg gagagagcat
     3841 gaacttcagt gatgtgttcg attccagtga ggactacttc gtgtccgtca atacatccta
     3901 ctacagcgta gattctgaga tgctgctttg ctccctgcag gaggtgcgcc agttctcccg
     3961 actcttcgtt cccatcgcgt attcactcat ctgcgtcttt ggcctcctgg ggaatatctt
     4021 ggtagtcatc acatttgctt tttataaaaa ggccagatcc atgacagacg tctacctgct
     4081 taatatggcc attgcagaca tcctgttcgt cctgacgctc ccattctggg ctgtgagtca
     4141 cgctaccggg gcctgggtgt tcagtaatgc aacctgcaag ctgttgaagg gaatttatgc
     4201 catcaacttt aattgcggta tgctgctgct gacttgcata agtatggaca gatacatcgc
     4261 catagtacag gcaacaaaat ccttcagact gaggtctcga acgctcccta ggtctaaaat
     4321 catttgtctt gttgtatggg gcttgagcgt gattatttcc agcagtactt tcgtgtttaa
     4381 ccagaagtac aacacgcagg gcagcgatgt gtgtgagccc aagtaccaga cggtatctga
     4441 gcctatcagg tggaagctgt tgatgctggg tttggaattg ctgttcggct tttttatccc
     4501 actcatgttt atgatttttt gttatacctt catcgtgaag accctggtcc aggcacaaaa
     4561 ctctaagcga cacaaggcca tccgggtaat catcgctgtt gtgctggtat ttctggcttg
     4621 ccagatccct cataatatgg tccttctggt aacggcagcc aacctgggca aaatgaatcg
     4681 ctcatgtcag tcagaaaagc ttatcgggta caccaaaacg gttacggagg ttctggcgtt
     4741 cctgcattgt tgccttaacc ctgtgctgta cgcatttata ggtcagaagt ttcgaaacta
     4801 cttcctgaag attctcaaag acctctggtg tgtaagaaga aagtataaaa gtagtggatt
     4861 ttcatgcgca ggacgctact ccgaaaacat ttccagacag accagtgaga cggccgataa
     4921 cgataacgcc tcctccttta ccatgaccgg tggacgcacc ccacccagcc tgggtcccca
     4981 agatgagtcc tgcaccaccg ccagctcctc cctggccaag gacacttcat cgggtaccgg
     5041 tgctttgcac ttggatcaaa cgccatctcg ccagcctatt cccagtgagg gtcttcagct
     5101 gcatttaccg caggttttag ctgacgctgt ctcacgcctg gtcctgggta agtttggtga
     5161 cctgaccgac aacttctcct cccctcacgc tcgcagaaaa gtgctggctg gagtcgtcat
     5221 gacaacaggc acagatgtta aagatgccaa ggtgataagt gtttctacag gaacaaaatg
     5281 tattaatggt gaatacatga gtgatcgtgg ccttgcatta aatgactgcc atgcagaaat
     5341 aatatctcgg agatccttgc tcagatttct ttatacacaa cttgagcttt acttaaataa
     5401 caaagatgat caaaaaagat ccatctttca gaaatcagag cgaggggggt ttaggctgaa
     5461 ggagaatgtc cagtttcatc tgtacatcag cacctctccc tgtggagatg ccagaatctt
     5521 ctcaccacat gagccaatcc tggaagaacc agcagataga cacccaaatc gtaaagcaag
     5581 aggacagcta cggaccaaaa tagagtctgg tcaggggacg attccagtgc gctccaatgc
     5641 gagcatccaa gcgtgggacg gggtgctgca aggggagcgg ctgctcacca tgtcctgcag
     5701 tgacaagatt gcacgctgga acgtggtggg catccaggga tccctgctca gcattttcgt
     5761 ggagcccatt tacttctcga gcatcatcct gggcagcctt taccacgggg accacctttc
     5821 cagggccatg taccagcgga tctccaacat agaggacctg ccacctctct acaccctcaa
     5881 caagcctttg ctcagtggca tcagcaatgc agaagcacgg cagccaggga aggcccccaa
     5941 cttcagtgtc aactggacgg taggcgactc cgctattgag gtcatcaacg ccacgactgg
     6001 gaaggatgag ctgggccgcg cgtcccgcct gtgtaagcac gcgttgtact gtcgctggat
     6061 gcgtgtgcac ggcaaggttc cctcccactt actacgctcc aagattacca aacccaacgt
     6121 gtaccatgag tccaagctgg cggcaaagga gtaccaggcc gccaaggcgc gtctgttcac
     6181 agccttcatc aaggcggggc tgggggcctg ggtggagaag cccaccgagc aggaccagtt
     6241 ctcactcacg ccctgatagc ccctctccct cccccccccc taacgttact ggccgaagcc
     6301 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     6361 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     6421 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     6481 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     6541 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     6601 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     6661 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     6721 ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     6781 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     6841 acaaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6901 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6961 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     7021 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg
     7081 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     7141 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     7201 aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca gggcaagggt
     7261 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     7321 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     7381 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     7441 gccgccacga acttctctct gttaaagcaa gcaggtgacg tggaagaaaa ccccggtccc
     7501 ggatccatgg tgtctaaggg cgaagagctg attaaggaga acatgcacat gaagctgtac
     7561 atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc
     7621 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc
     7681 ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag
     7741 ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc
     7801 acatacgagg acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
     7861 ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag
     7921 aagaaaacac tcggctggga ggccttcacc gaaacgctgt accccgctga cggcggcctg
     7981 gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacgcc
     8041 aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat
     8101 gtggactaca gactggaaag aatcaaggag gccaacaacg aaacctacgt cgagcagcac
     8161 gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa acttaattaa
     8221 gaattcctcg agctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     8281 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     8341 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     8401 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     8461 gggacgacgt caggtggcac ttttcgatat ctataacaag aaaatatata tataataagt
     8521 tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa atcttaaaag
     8581 tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt tcaaaatcag
     8641 tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga ctgagatgtc
     8701 ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc
     8761 atgcgtcaat tttacgcaga ctatctttct agggttaatc tagctgcatc aggatcatat
     8821 cgtcgggtct tttttccggc tcagtcatcg cccaagctgg cgctatctgg gcatcgggga
     8881 ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt ttgccgagga
     8941 tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt accatgatga ttcgggaagg
     9001 tgtggccatg cacgccttta acggtgaact gttcgttcag gccacctggg ataccagttc
     9061 gtcgcggctt ttccggacac agttccggat ggtcagcccg aagcgcatca gcaacccgaa
     9121 caataccggc gacagccgga actgccgtgc cggtgtgcag attaatgaca gcggtgcggc
     9181 gctgggatat tacgtcagcg aggacgggta tcctggctgg atgccgcaga aatggacatg
     9241 gataccccgt gagttacccg gcgggcgcgc ttggcgtaat catggtcata gctgtttcct
     9301 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     9361 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     9421 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     9481 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9541 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9601 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9661 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9721 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9781 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9841 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9901 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9961 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10021 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10081 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    10141 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10201 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10261 aaaaaaggat ctcaa
//
