LOCUS       sequence-467440-        9355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac donor plasmid to overexpress a full length ADAR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9355)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 9355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP
FEATURES             Location/Qualifiers
     source          1..9355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1905..1967
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2550..3728
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2781..3719
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3676..3696
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3737..3746
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5867..5920
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5927..6523
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5927..5946)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6423..6443
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6524..6580
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6587..7297
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(7331..7348)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7337..7544
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7840..7874
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8365..8381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8365..8381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8378..8400)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8389..8405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8413..8419,8420..8437,8438..8443))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8458..8479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8596..8613)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8767..9355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8847..8866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
     1801 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
     1861 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
     1921 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc
     1981 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
     2041 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
     2101 caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc
     2161 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
     2221 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
     2281 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
     2341 gagcctgcag acacctgggg ggatacgggg aaaaggcctc cacggccact agtttcgcac
     2401 gattgcacct tgggagcctt aaccgctttt gaatgtccgt cttagttaat ccctaattga
     2461 acgctccaaa aggtgacttc caatagggat ttattccttt taaaattaac ggcataatcg
     2521 tgccgccgaa tgcaacctcg atcgagccgg gctccggtgc ccgtcagtgg gcagagcgca
     2581 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2641 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2701 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2761 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     2821 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     2881 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     2941 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     3001 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     3061 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     3121 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     3181 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     3241 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     3301 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     3361 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     3421 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     3481 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     3541 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     3601 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     3661 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     3721 tgtcgtgaat ctagaagcca ccatgggtct agtagatata gaagatgaag aaaacatgag
     3781 ttccagcagc actgatgtga aggaaaaccg caatctggac aacgtgtccc ccaaggatgg
     3841 cagcacacct gggcctggcg agggctctca gctctccaat gggggtggtg gtggccccgg
     3901 cagaaagcgg cccctggagg agggcagcaa tggccactcc aagtaccgcc tgaagaaaag
     3961 gaggaaaaca ccagggcccg tcctccccaa gaacgccctg atgcagctga atgagatcaa
     4021 gcctggtttg cagtacacac tcctgtccca gactgggccc gtgcacgcgc ctttgtttgt
     4081 catgtctgtg gaggtgaatg gccaggtttt tgagggctct ggtcccacaa agaaaaaggc
     4141 aaaactccat gctgctgaga aggccttgag gtctttcgtt cagtttccta atgcctctga
     4201 ggcccacctg gccatgggga ggaccctgtc tgtcaacacg gacttcacat ctgaccaggc
     4261 cgacttccct gacacgctct tcaatggttt tgaaactcct gacaaggcgg agcctccctt
     4321 ttacgtgggc tccaatgggg atgactcctt cagttccagc ggggacctca gcttgtctgc
     4381 ttccccggtg cctgccagcc tagcccagcc tcctctccct gtcttaccac cattcccacc
     4441 cccgagtggg aagaatcccg tgatgatctt gaacgaactg cgcccaggac tcaagtatga
     4501 cttcctctcc gagagcgggg agagccatgc caagagcttc gtcatgtctg tggtcgtgga
     4561 tggtcagttc tttgaaggct cggggagaaa caagaagctt gccaaggccc gggctgcgca
     4621 gtctgccctg gccgccattt ttaacttgca cttggatcag acgccatctc gccagcctat
     4681 tcccagtgag ggtcttcagc tgcatttacc gcaggtttta gctgacgctg tctcacgcct
     4741 ggtcctgggt aagtttggtg acctgaccga caacttctcc tcccctcacg ctcgcagaaa
     4801 agtgctggct ggagtcgtca tgacaacagg cacagatgtt aaagatgcca aggtgataag
     4861 tgtttctaca ggaacaaaat gtattaatgg tgaatacatg agtgatcgtg gccttgcatt
     4921 aaatgactgc catgcagaaa taatatctcg gagatccttg ctcagatttc tttatacaca
     4981 acttgagctt tacttaaata acaaagatga tcaaaaaaga tccatctttc agaaatcaga
     5041 gcgagggggg tttaggctga aggagaatgt ccagtttcat ctgtacatca gcacctctcc
     5101 ctgtggagat gccagaatct tctcaccaca tgagccaatc ctggaagaac cagcagatag
     5161 acacccaaat cgtaaagcaa gaggacagct acggaccaaa atagagtctg gtgaggggac
     5221 gattccagtg cgctccaatg cgagcatcca aacgtgggac ggggtgctgc aaggggagcg
     5281 gctgctcacc atgtcctgca gtgacaagat tgcacgctgg aacgtggtgg gcatccaggg
     5341 atccctgctc agcattttcg tggagcccat ttacttctcg agcatcatcc tgggcagcct
     5401 ttaccacggg gaccaccttt ccagggccat gtaccagcgg atctccaaca tagaggacct
     5461 gccacctctc tacaccctca acaagccttt gctcagtggc atcagcaatg cagaagcacg
     5521 gcagccaggg aaggccccca acttcagtgt caactggacg gtaggcgact ccgctattga
     5581 ggtcatcaac gccacgactg ggaaggatga gctgggccgc gcgtcccgcc tgtgtaagca
     5641 cgcgttgtac tgtcgctgga tgcgtgtgca cggcaaggtt ccctcccact tactacgctc
     5701 caagattacc aaacccaacg tgtaccatga gtccaagctg gcggcaaagg agtaccaggc
     5761 cgccaaggcg cgtctgttca cagccttcat caaggcgggg ctgggggcct gggtggagaa
     5821 gcccaccgag caggaccagt tctcactcac gccactagtc gctagcgaag gtcgggggag
     5881 tttgcttacc tgtggcgatg tcgaggagaa tccagggccc aagcttatga ccgagtacaa
     5941 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     6001 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     6061 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     6121 ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg
     6181 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     6241 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     6301 ggccaccgtc ggagtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     6361 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     6421 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6481 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccgccacga acttctctct
     6541 gttaaagcaa gcaggtgacg tggaagaaaa ccccggtccc ggatccatgg tgtctaaggg
     6601 cgaagagctg attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa
     6661 ccatcacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat
     6721 gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag
     6781 cttcctctac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa
     6841 gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgagg acgggggcgt
     6901 gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat
     6961 cagaggggtg aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga
     7021 ggccttcacc gaaacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc
     7081 cctgaagctc gtgggcggga gccatctgat cgcaaacgcc aagaccacat atagatccaa
     7141 gaaacccgct aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag
     7201 aatcaaggag gccaacaacg aaacctacgt cgagcagcac gaggtggcag tggccagata
     7261 ctgcgacctc cctagcaaac tggggcacaa acttaattaa gaattcctcg agctagagct
     7321 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     7381 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     7441 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     7501 agcaaggggg aggattggga agacaatagc aggcatgctg gggacgacgt caggtggcac
     7561 ttttcgatat ctataacaag aaaatatata tataataagt tatcacgtaa gtagaacatg
     7621 aaataacaat ataattatcg tatgagttaa atcttaaaag tcacgtaaaa gataatcatg
     7681 cgtcattttg actcacgcgg tcgttatagt tcaaaatcag tgacacttac cgcattgaca
     7741 agcacgcctc acgggagctc caagcggcga ctgagatgtc ctaaatgcac agcgacggat
     7801 tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga
     7861 ctatctttct agggttaatc tagctgcatc aggatcatat cgtcgggtct tttttccggc
     7921 tcagtcatcg cccaagctgg cgctatctgg gcatcgggga ggaagaagcc cgtgcctttt
     7981 cccgcgaggt tgaagcggca tggaaagagt ttgccgagga tgactgctgc tgcattgacg
     8041 ttgagcgaaa acgcacgttt accatgatga ttcgggaagg tgtggccatg cacgccttta
     8101 acggtgaact gttcgttcag gccacctggg ataccagttc gtcgcggctt ttccggacac
     8161 agttccggat ggtcagcccg aagcgcatca gcaacccgaa caataccggc gacagccgga
     8221 actgccgtgc cggtgtgcag attaatgaca gcggtgcggc gctgggatat tacgtcagcg
     8281 aggacgggta tcctggctgg atgccgcaga aatggacatg gataccccgt gagttacccg
     8341 gcgggcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8401 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8461 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8521 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8581 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8641 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8701 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8761 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8821 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8881 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8941 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9001 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9061 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9121 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9181 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9241 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9301 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaa
//
