LOCUS       sequence-467443-        9660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac donor plasmid to overexpress FKBP-TM-ADAR2dd (eLIDAR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9660)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 9660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: LSM0251 (PB)EF1a-FKBP-tm-ADAR2ddIRESv0-PuroR-P...
FEATURES             Location/Qualifiers
     source          1..9660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1905..1967
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2550..3728
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2781..3719
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3676..3696
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3737..3746
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3812..3814,3815..4135)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     misc_feature    join(5645..6219,6220..6222,6223..6231)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5812..5829)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6039..6058
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6232..6828
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6232..6251)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6728..6748
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6829..6885
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6892..7602
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(7636..7653)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7642..7849
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8145..8179
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8670..8686)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8670..8686)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8683..8705)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8694..8710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8718..8724,8725..8742,8743..8748))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8763..8784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8901..8918)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9072..9660)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9152..9171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
     1801 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
     1861 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
     1921 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc
     1981 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
     2041 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
     2101 caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc
     2161 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
     2221 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
     2281 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
     2341 gagcctgcag acacctgggg ggatacgggg aaaaggcctc cacggccact agtttcgcac
     2401 gattgcacct tgggagcctt aaccgctttt gaatgtccgt cttagttaat ccctaattga
     2461 acgctccaaa aggtgacttc caatagggat ttattccttt taaaattaac ggcataatcg
     2521 tgccgccgaa tgcaacctcg atcgagccgg gctccggtgc ccgtcagtgg gcagagcgca
     2581 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2641 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2701 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2761 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     2821 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     2881 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     2941 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     3001 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     3061 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     3121 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     3181 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     3241 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     3301 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     3361 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     3421 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     3481 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     3541 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     3601 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     3661 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     3721 tgtcgtgaat ctagaagcca ccatggcgct ccctgtcacc gcactgcttc ttccgctggc
     3781 actgctgctg cacgctgcac ggcctggatc catgggagtg caggtggaaa ccatctcccc
     3841 aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact acaccgggat
     3901 gcttgaagat ggaaagaaat ttgattcctc ccgggacaga aacaagccct ttaagtttat
     3961 gctaggcaag caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg
     4021 tcagagagcc aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg
     4081 catcatccca ccacatgcca ctctcgtctt cgatgtggag cttctaaaac tggaaggcgc
     4141 cgggacagga gggtctgggg gtggcactgg cggatctgga ggagactaca aggacgatga
     4201 cgacaacggc ggatcggggg ggtctggtgg tagtggcggt tcggggggag gtaccggtct
     4261 ggtcgtggtt gctggagtcc tgttttgtta tggcttgcta gtgacagtgg ctctttgtgt
     4321 tgaaaattcc gggtcaggcg gttccggcag cggggcgggt agcgggtcca gtgggtcaga
     4381 aacacccggc acgagtgagt cagcaacgcc agagagtgct agcggtgctt tgcacttgga
     4441 tcaaacgcca tctcgccagc ctattcccag tgagggtctt cagctgcatt taccgcaggt
     4501 tttagctgac gctgtctcac gcctggtcct gggtaagttt ggtgacctga ccgacaactt
     4561 ctcctcccct cacgctcgca gaaaagtgct ggctggagtc gtcatgacaa caggcacaga
     4621 tgttaaagat gccaaggtga taagtgtttc tacaggaaca aaatgtatta atggtgaata
     4681 catgagtgat cgtggccttg cattaaatga ctgccatgca gaaataatat ctcggagatc
     4741 cttgctcaga tttctttata cacaacttga gctttactta aataacaaag atgatcaaaa
     4801 aagatccatc tttcagaaat cagagcgagg ggggtttagg ctgaaggaga atgtccagtt
     4861 tcatctgtac atcagcacct ctccctgtgg agatgccaga atcttctcac cacatgagcc
     4921 aatcctggaa gaaccagcag atagacaccc aaatcgtaaa gcaagaggac agctacggac
     4981 caaaatagag tctggtcagg ggacgattcc agtgcgctcc aatgcgagca tccaagcgtg
     5041 ggacggggtg ctgcaagggg agcggctgct caccatgtcc tgcagtgaca agattgcacg
     5101 ctggaacgtg gtgggcatcc agggatccct gctcagcatt ttcgtggagc ccatttactt
     5161 ctcgagcatc atcctgggca gcctttacca cggggaccac ctttccaggg ccatgtacca
     5221 gcggatctcc aacatagagg acctgccacc tctctacacc ctcaacaagc ctttgctcag
     5281 tggcatcagc aatgcagaag cacggcagcc agggaaggcc cccaacttca gtgtcaactg
     5341 gacggtaggc gactccgcta ttgaggtcat caacgccacg actgggaagg atgagctggg
     5401 ccgcgcgtcc cgcctgtgta agcacgcgtt gtactgtcgc tggatgcgtg tgcacggcaa
     5461 ggttccctcc cacttactac gctccaagat taccaaaccc aacgtgtacc atgagtccaa
     5521 gctggcggca aaggagtacc aggccgccaa ggcgcgtctg ttcacagcct tcatcaaggc
     5581 ggggctgggg gcctgggtgg agaagcccac cgagcaggac cagttctcac tcacgccctg
     5641 atagcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     5701 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     5761 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     5821 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     5881 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     5941 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     6001 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     6061 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     6121 ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     6181 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catgaccgag
     6241 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     6301 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     6361 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     6421 gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa
     6481 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     6541 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     6601 ttcctggcca ccgtcggagt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     6661 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     6721 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     6781 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgc cacgaacttc
     6841 tctctgttaa agcaagcagg tgacgtggaa gaaaaccccg gtcccggatc catggtgtct
     6901 aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     6961 gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     7021 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     7081 actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc
     7141 ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaggacggg
     7201 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     7261 aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc
     7321 tgggaggcct tcaccgaaac gctgtacccc gctgacggcg gcctggaagg cagaaacgac
     7381 atggccctga agctcgtggg cgggagccat ctgatcgcaa acgccaagac cacatataga
     7441 tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg
     7501 gaaagaatca aggaggccaa caacgaaacc tacgtcgagc agcacgaggt ggcagtggcc
     7561 agatactgcg acctccctag caaactgggg cacaaactta attaagaatt cctcgagcta
     7621 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     7681 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     7741 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     7801 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggac gacgtcaggt
     7861 ggcacttttc gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga
     7921 acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa
     7981 tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat
     8041 tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga
     8101 cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac
     8161 gcagactatc tttctagggt taatctagct gcatcaggat catatcgtcg ggtctttttt
     8221 ccggctcagt catcgcccaa gctggcgcta tctgggcatc ggggaggaag aagcccgtgc
     8281 cttttcccgc gaggttgaag cggcatggaa agagtttgcc gaggatgact gctgctgcat
     8341 tgacgttgag cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc
     8401 ctttaacggt gaactgttcg ttcaggccac ctgggatacc agttcgtcgc ggcttttccg
     8461 gacacagttc cggatggtca gcccgaagcg catcagcaac ccgaacaata ccggcgacag
     8521 ccggaactgc cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg gatattacgt
     8581 cagcgaggac gggtatcctg gctggatgcc gcagaaatgg acatggatac cccgtgagtt
     8641 acccggcggg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8701 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8761 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8821 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8881 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8941 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9001 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9061 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9121 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9181 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9241 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9301 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9361 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9421 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9481 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9541 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9601 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
//
