LOCUS       sequence-467445-        9868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express hPRMT7 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Lee SH, Chen TY, Dhar SS, Gu B, Chen K, Kim YZ, Li W, Lee MG
  TITLE     A feedback loop comprising PRMT7 and miR-24-2 interplays with Oct4, 
            Nanog, Klf4 and c-Myc to regulate stemness.
  JOURNAL   Nucleic Acids Res. 2016 Dec 15;44(22):10603-10618. Epub 2016 Sep 12.
  PUBMED    27625395
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-12-15"; volume: "44(22)"; pages: 
            "10603-10618. Epub 2016 Sep 12"
COMMENT     Sequence Label: pCDH1-hPRMT7-GFP
FEATURES             Location/Qualifiers
     source          1..9868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..247
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             260..528
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    2659..3231
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2826..2843)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3053..3072
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3264..3926
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     misc_feature    4017..4605
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4070..4090)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4488..4499)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4679..4912
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4984..5105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5021..5040)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5075..5094
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5145..5280
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5313..5329)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5313..5329)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5326..5348)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5337..5353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5361..5367,5368..5385,5386..5391))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5406..5427
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5544..5561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5715..6303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(6474..7265,7266..7334))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7097..7116
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7335..7439)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7507..7525
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7563..7585)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7685..7704
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7898..7920
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7912..7929
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7913..7929
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7944..8170
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8171..8351
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8398..8523
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9016..9249
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9434..9478
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9745..9861
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctagtggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
       61 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
      121 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      181 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
      241 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
      301 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
      361 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
      421 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
      481 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
      541 ggcgcctact ctagagctag cgaattcgcc accatgaaga tcttctgcag tcgggccaat
      601 ccgaccacgg ggtctgtgga gtggctggag gaggatgaac actatgatta ccaccaggag
      661 attgcaaggt catcttatgc agatatgcta catgacaaag acagaaatgt aaaatactac
      721 caaggtatcc gggctgccgt gagcagggtg aaggacagag gacagaaggc cttggttctc
      781 gacattggca ctggcacggg actcttgtca atgatggcgg tcacagcagg tgccgacttc
      841 tgctatgcca tcgaggtttt caagcctatg gctgatgctg ctgtgaagat tgtggagaaa
      901 aatggcttta gtgataagat taaggttatc aacaagcatt ccaccgaggt gactgtaggt
      961 ccagagggtg acatgccatg ccgtgccaac atcctggtca cagagttgtt tgacacagag
     1021 ctgatcgggg agggggcgct gccctcctat gagcacgcac acaggcatct cgtggaggaa
     1081 aattgtgagg ccgtgcccca cagagccacc gtctatgcac agctggtgga gtccgggagg
     1141 atgtggtcgt ggaacaagct atttcccatc cacgtgcaga ccagcctcgg agagcaggtc
     1201 atcgtccctc ccgttgacgt ggagagctgc cctggcgcac cctctgtctg tgacattcag
     1261 ctgaaccagg tgtcaccagc cgactttaca gtcctcagcg atgtgctgcc catgttcagc
     1321 atagacttca gcaagcaagt cagtagctca gcagcctgcc atagcaggcg gtttgaacct
     1381 ctgacatctg gccgagctca ggtggttctc tcgtggtggg acattgaaat ggaccctgag
     1441 gggaagatca agtgcaccat ggcccccttc tgggcacact cagacccaga ggagatgcag
     1501 tggcgggacc actggatgca gtgtgtgtac ttcctgccac aagaggagcc tgtggtgcag
     1561 ggctcagcgc tctatctggt agcccaccac gatgactact gcgtatggta cagcctgcag
     1621 aggaccagcc ctgaaaagaa tgagagagtc cgccagatgc gccccgtgtg tgactgccag
     1681 gctcacctgc tctggaaccg gcctcggttt ggagagatca atgaccagga cagaactgat
     1741 cgatacgtcc aggctctgag gaccgtgctg aagccagaca gcgtgtgcct gtgtgtcagc
     1801 gatggcagcc tgctctccgt gctggcccat cacctggggg tggagcaggt gtttacagtc
     1861 gagagttcag cagcttctca caaactgttg agaaaaatct tcaaggctaa ccacttggaa
     1921 gataaaatta acatcataga gaaacggccg gaattattaa caaatgagga cctacagggc
     1981 agaaaggtct ctctcctcct gggcgagccg ttcttcacta ccagcctgct gccgtggcac
     2041 aacctctact tctggtacgt gcggaccgct gtggaccagc acctggggcc aggtgccatg
     2101 gtgatgcccc aggcagcctc gctgcacgct gtggttgtgg agttcaggga cctgtggcgg
     2161 atccggagcc cctgtggtga ctgcgaaggc ttcgacgtgc acatcatgga cgacatgatt
     2221 aagcgtgccc tggacttcag ggagagcagg gaagctgagc cccacccgct gtgggagtac
     2281 ccatgccgca gcctctccga gccctggcag atcctgacct ttgacttcca gcagccggtg
     2341 cccctgcagc ccctgtgtgc cgagggcact gtggagctca gaaggcccgg gcagagccac
     2401 gcagcggtgc tatggatgga gtaccacctg accccggagt gcacgctcag cactggcctc
     2461 ctggagcctg cagaccccga ggggggctgc tgctggaacc cccactgcaa gcaggccgtc
     2521 tacttcttca gccctgcccc agatcccaga gcactgctgg gtggcccacg gactgtcagc
     2581 tatgcagtgg agtttcaccc cgacacaggc gacatcatca tggagttcag gcatgcagat
     2641 accccagacg cggccgcgcc cctctccctc ccccccccct aacgttactg gccgaagccg
     2701 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     2761 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     2821 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     2881 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     2941 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     3001 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     3061 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     3121 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     3181 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgataat acctccggaa
     3241 tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc accggcaccc
     3301 tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag cagggccgca
     3361 tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac ctgctgagcc
     3421 acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac gagaacccct
     3481 tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag tacgaggacg
     3541 gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg atcggcgact
     3601 tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac aagatcatcc
     3661 gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg gtgggcagct
     3721 tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg gacagccaca
     3781 tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc atgttcgcct
     3841 tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag taccagcacg
     3901 ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg
     3961 acggcaccgc cggacccggc tccaccggat ctcgctagag ctgaatctaa gtcgacaatc
     4021 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4081 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4141 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4201 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4261 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4321 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4381 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     4441 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4501 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     4561 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga
     4621 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     4681 gaagggctaa ttcactccca acgaagataa gatctgcttt ttgcttgtac tgggtctctc
     4741 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4801 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4861 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     4921 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     4981 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5041 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5101 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     5161 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5221 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5281 tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt
     5341 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5401 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5461 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5521 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5581 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5641 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5701 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5761 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5821 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5881 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5941 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6001 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6061 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6121 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6181 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6241 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6301 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6361 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6421 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6481 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6541 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6601 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6661 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6721 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     6781 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6841 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     6901 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     6961 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7021 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7081 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7141 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7201 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7261 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7321 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7381 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7441 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     7501 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     7561 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     7621 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     7681 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     7741 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     7801 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     7861 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     7921 acggccagtg ccaagctgac gcgtgtagtc ttatgcaata ctcttgtagt cttgcaacat
     7981 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     8041 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     8101 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     8161 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8221 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8281 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8341 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     8401 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     8461 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     8521 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     8581 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     8641 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     8701 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     8761 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     8821 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccactga tcttcagacc
     8881 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     8941 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     9001 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     9061 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     9121 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     9181 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     9241 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     9301 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     9361 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     9421 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     9481 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     9541 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     9601 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     9661 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     9721 cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
     9781 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     9841 aattacaaaa ttcaaaattt tatcgata
//
