LOCUS       sequence-467449-       18763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCCI vector encoding YFV-17D lacking C, prM, E and NS1 proteins but 
            with Flpo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18763)
  AUTHORS   Li Y, Xu W
  TITLE     Xu Lab plasmids 2025
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 18763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pCCI-YFV-Delta-CMENS1-Flpo
FEATURES             Location/Qualifiers
     source          1..18763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    9..42
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    60..458
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_feature    718..1191
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(1264..2235)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(2235..3410)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    3736..3986
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3989..4744)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      4835..5054
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      5122..5653
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      5130..5744
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        6602..6704
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             6705..7364
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6771..6790)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    7584..7605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7620..7625,7626..7643,7644..7650)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7658..7674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7663..7685
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7682..7698
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7682..7698
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8135..8153
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8135..8152
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     18052..18071
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(18435..18454)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(18436..18454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(18461..18478)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(18461..18477)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18470..18492)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(18685..18704)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 catcgaatat aacttcgtat aatgtatgct atacgaagtt attagcgatg agctcggact
       61 tccattgttc attccacgga caaaaacaga gaaaggaaac gacagaggcc aaaaagctcg
      121 ctttcagcac ctgtcgtttc ctttcttttc agagggtatt ttaaataaaa acattaagtt
      181 atgacgaaga agaacggaaa cgccttaaac cggaaaattt tcataaatag cgaaaacccg
      241 cgaggtcgcc gccccgtaac ctgtcggatc accggaaagg acccgtaaag tgataatgat
      301 tatcatctac atatcacaac gtgcgtggag gccatcaaac cacgtcaaat aatcaattat
      361 gacgcaggta tcgtattaat tgatctgcat caacttaacg taaaagcaac ttcagacaat
      421 acaaatcagc gacactgaat acggggcaac ctcatgtccg agctcgcgag ctcgtcgaca
      481 gcgacacact tgcatcggat gcagcccggt taacgtgccg gcacggcctg ggtaaccagg
      541 tattttgtcc acataaccgt gcgcaaaatg ttgtggataa gcaggacaca gcagcaatcc
      601 acagcaggca tacaaccgca caccgaggtt actccgttct acaggttacg acgacatgtc
      661 aatacttgcc cttgacaggc attgatggaa tcgtagtctc acgctgatag tctgatcgac
      721 aatacaagtg ggaccgtggt cccagaccga taatcagacc gacaacacga gtgggatcgt
      781 ggtcccagac taataatcag accgacgata cgagtgggac cgtggtccca gactaataat
      841 cagaccgacg atacgagtgg gaccgtggtt ccagactaat aatcagaccg acgatacgag
      901 tgggaccgtg gtcccagact aataatcaga ccgacgatac gagtgggacc atggtcccag
      961 actaataatc agaccgacga tacgagtggg accgtggtcc cagtctgatt atcagaccga
     1021 cgatacgagt gggaccgtgg tcccagacta ataatcagac cgacgatacg agtgggaccg
     1081 tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc agtctgatta
     1141 tcagaccgac gatacaagtg gaacagtggg cccagagaga atattcaggc cagttatgct
     1201 ttctggcctg taacaaagga cattaagtaa agacagataa acgtagacta aaacgtggtc
     1261 gcatcagggt gctggctttt caagttcctt aagaatggcc tcaattttct ctatacactc
     1321 agttggaaca cgagacctgt ccaggttaag caccatttta tcgcccttat acaatactgt
     1381 cgctccagga gcaaactgat gtcgtgagct taaactagtt cttgatgcag atgacgtttt
     1441 aagcacagaa gttaaaagag tgataacttc ttcagcttca aatatcaccc cagctttttt
     1501 ctgctcatga aggttagatg cctgctgctt aagtaattcc tctttatctg taaaggcttt
     1561 ttgaagtgca tcacctgacc gggcagatag ttcaccgggg tgagaaaaaa gagcaacaac
     1621 tgatttaggc aatttggcgg tgttgataca gcgggtaata atcttacgtg aaatattttc
     1681 cgcatcagcc agcgcagaaa tatttccagc aaattcattc tgcaatcggc ttgcataacg
     1741 ctgaccacgt tcataagcac ttgttgggcg ataatcgtta cccaatctgg ataatgcagc
     1801 catctgctca tcatccagct cgccaaccag aacacgataa tcactttcgg taagtgcagc
     1861 agctttacga cggcgactcc catcggcaat ttctatgaca ccagatactc ttcgaccgaa
     1921 cgccggtgtc tgttgaccag tcagtagaaa agaagggatg agatcatcca gtgcgtcctc
     1981 agtaagcagc tcctggtcac gttcattacc tgaccatacc cgagaggtct tctcaacact
     2041 atcaccccgg agcacttcaa gagtaaactt cacatcccga ccacatacag gcaaagtaat
     2101 ggcattaccg cgagccatta ctcctacgcg cgcaattaac gaatccacca tcggggcagc
     2161 tggtgtcgat aacgaagtat cttcaaccgg ttgagtattg agcgtatgtt ttggaataac
     2221 aggcgcacgc ttcattatct aatctcccag cgtggtttaa tcagacgatc gaaaatttca
     2281 ttgcagacag gttcccaaat agaaagagca tttctccagg caccagttga agagcgttga
     2341 tcaatggcct gttcaaaaac agttctcatc cggatctgac ctttaccaac ttcatccgtt
     2401 tcacgtacaa cattttttag aaccatgctt ccccaggcat cccgaatttg ctcctccatc
     2461 cacggggact gagagccatt actattgctg tatttggtaa gcaaaatacg tacatcaggc
     2521 tcgaaccctt taagatcaac gttcttgagc agatcacgaa gcatatcgaa aaactgcagt
     2581 gcggaggtgt agtcaaacaa ctcagcaggc gtgggaacaa tcagcacatc agcagcacat
     2641 acgacattaa tcgtgccgat acccaggtta ggcgcgctgt caataactat gacatcatag
     2701 tcatgagcaa cagtttcaat ggccagtcgg agcatcaggt gtggatcggt gggcagttta
     2761 ccttcatcaa atttgcccat taactcagtt tcaatacggt gcagagccag acaggaagga
     2821 ataatgtcaa gccccggcca gcaagtgggc tttattgcat aagtgacatc gtccttttcc
     2881 ccaagataga aaggcaggag agtgtcttct gcatgaatat gaagatctgg tacccatccg
     2941 tgatacattg aggctgttcc ctgggggtcg ttaccttcca cgagcaaaac acgtagcccc
     3001 ttcagagcca gatcctgagc aagatgaaca gaaactgagg ttttgtaaac gccaccttta
     3061 tgggcagcaa ccccgatcac cggtggaaat acgtcttcag cacgtcgcaa tcgcgtacca
     3121 aacacatcac gcatatgatt aatttgttca attgtataac caacacgttg ctcaacccgt
     3181 cctcgaattt ccatatccgg gtgcggtagt cgccctgctt tctcggcatc tctgatagcc
     3241 tgagaagaaa ccccaactaa atccgctgct tcacctattc tccagcgccg ggttattttc
     3301 ctcgcttccg ggctgtcatc attaaactgt gcaatggcga tagccttcgt catttcatga
     3361 ccagcgttta tgcactggtt aagtgtttcc atgagtttca ttctgaacat cctttaatca
     3421 ttgctttgcg tttttttatt aaatcttgca atttactgca aagcaacaac aaaatcgcaa
     3481 agtcatcaaa aaaccgcaaa gttgtttaaa ataagagcaa cactacaaaa ggagataaga
     3541 agagcacata cctcagtcac ttattatcac tagcgctcgc cgcagccgtg taaccgagca
     3601 tagcgagcga actggcgagg aagcaaagaa gaactgttct gtcagatagc tcttacgctc
     3661 agcgcaagaa gaaatatcca ccgtgggaaa aactccaggt agaggtacac acgcggatag
     3721 ccaattcaga gtaataaact gtgataatca accctcatca atgatgacga actaaccccc
     3781 gatatcaagt cacatgacga agggaaagag aaggaaatca actgtgacaa actgccctca
     3841 aatttggctt ccttaaaaat tacagttcaa aaagtatgag aaaatccatg caggctgaag
     3901 gaaacagcaa aactgtgaca aattaccctc agtaggtcag aacaaatgtg acgaaccacc
     3961 ctcaaatctg tgacagataa ccctcagact atcctgtcgt catggaagtg atatcgcgga
     4021 aggaaaatac gatatgagtc gtctggcggc ctttcttttt ctcaatgtat gagaggcgca
     4081 ttggagttct gctgttgatc tcattaacac agacctgcag gaagcggcgg cggaagtcag
     4141 gcatacgctg gtaactttga ggcagctggt aacgctctat gatccagtcg attttcagag
     4201 agacgatgcc tgagccatcc ggcttacgat actgacacag ggattcgtat aaacgcatgg
     4261 catacggatt ggtgatttct tttgtttcac taagccgaaa ctgcgtaaac cggttctgta
     4321 acccgataaa gaagggaatg agatatgggt tgatatgtac actgtaaagc cctctggatg
     4381 gactgtgcgc acgtttgata aaccaaggaa aagattcata gcctttttca tcgccggcat
     4441 cctcttcagg gcgataaaaa accacttcct tccccgcgaa actcttcaat gcctgccgta
     4501 tatccttact ggcttccgca gaggtcaatc cgaatatttc agcatattta gcaacatgga
     4561 tctcgcagat accgtcatgt tcctgtaggg tgccatcaga ttttctgatc tggtcaacga
     4621 acagatacag catacgtttt tgatcccggg agagactata tgccgcctca gtgaggtcgt
     4681 ttgactggac gattcgcggg ctatttttac gtttcttgtg attgataacc gctgtttccg
     4741 ccatgacaga tccatgtgaa gtgtgacaag tttttagatt gtcacactaa ataaaaaaga
     4801 gtcaataagc agggataact ttgtgaaaaa acagcttctt ctgagggcaa tttgtcacag
     4861 ggttaagggc aatttgtcac agacaggact gtcatttgag ggtgatttgt cacactgaaa
     4921 gggcaatttg tcacaacacc ttctctagaa ccagcatgga taaaggccta caaggcgctc
     4981 taaaaaagaa gatctaaaaa ctataaaaaa aataattata aaaatatccc cgtggataag
     5041 tggataaccc caagggaagt tttttcaggc atcgtgtgta agcagaatat ataagtgctg
     5101 ttccctggtg cttcctcgct cactcgaccg ggagggttcg agaagggggg gcacccccct
     5161 tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata
     5221 ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct
     5281 tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc
     5341 atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg
     5401 cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg
     5461 ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg
     5521 ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc
     5581 aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg aggtatccac
     5641 aacgccggcg gccggccgcg gtgtctcgca cacggcttcg acggcgtttc tggcgcgttt
     5701 gcagggccat agacggccgc cagcccagcg gcgagggcaa ccagccgagg gcttcgccct
     5761 gtcgctcgac tgcggcgagc actactggct gtaaaaggac agaccacatc atggttctgt
     5821 gttcattagg ttgttctgtc cattgctgac ataatccgct ccacttcaac gtaacaccgc
     5881 acgaagattt ctattgttcc tgaaggcata ttcaaatcgt tttcgttacc gcttgcaggc
     5941 atcatgacag aacactactt cctataaacg ctacacaggc tcctgagatt aataatgcgg
     6001 atctctacga taatgggaga ttttcccgac tgtttcgttc gcttctcagt ggataacagc
     6061 cagcttctct gtttaacaga caaaaacagc atatccactc agttccacat ttccatataa
     6121 aggccaaggc atttattctc aggataattg tttcagcatc gcaaccgcat cagactccgg
     6181 catcgcaaac tgcacccggt gccgggcagc cacatccagc gcaaaaacct tcgtgtagac
     6241 ttccgttgaa ctgatggact tatgtcccat caggctttgc agaactttca gcggtatacc
     6301 ggcatacagc atgtgcatcg cataggaatg gcggaacgta tgtggtgtga ccggaacaga
     6361 gaacgtcaca ccgtcagcag cagcggcggc aaccgcctcc ccaatccagg tcctgaccgt
     6421 tctgtccgtc acttcccaga tccgcgcttt ctctgtcctt cctgtgcgac ggttacgccg
     6481 ctccatgagc ttatcgcgaa taaatacctg tgacggaaga tcacttcgca gaataaataa
     6541 atcctggtgt ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg
     6601 ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     6661 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     6721 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     6781 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     6841 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     6901 tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg
     6961 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     7021 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     7081 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     7141 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     7201 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     7261 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     7321 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt
     7381 attggtgccc ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg
     7441 gcagaaattc gatgataagc tgtcaaacat gagaattggt cgacggcccg ggcggccgca
     7501 aggagttcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7561 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7621 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7681 acaggaaaca gctatgacca tgattacgcc aagctattta ggtgagacta tagaatactc
     7741 aagcttgcat gcctgcaggt cgactctaga ggatcccaca ttattgaagc atttatcagg
     7801 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7861 ttccgcgcac atttccccga aaagtgccac ctgacgtgtc gacgcggccg ctagcgatga
     7921 ccctgctgat tggttcgctg accatttccg ggtgcgggac ggcgttacca gaaactcaga
     7981 aggttcgtcc aaccaaaccg actctgacgg cagtttacga gagagatgat agggtctgct
     8041 tcagtaagcc agatgctaca caattaggct tgtacatatt gtcgttagaa cgcggctaca
     8101 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agagtaaatc
     8161 ctgtgtgcta attgaggtgc attggtctgc aaatcgagtt gctaggcaat aaacacattt
     8221 ggattaattt taatcgttcg ttgagcgatt agcagagaac tgaccagaac atgtctggtc
     8281 gtaaagctca gggaaaaacc ctgggcgtca atatggtacg acgaggagtt cgctccttgt
     8341 caaacgagct cagccagttc gacatcctgt gcaagacccc ccccaaggtg ctggtgcggc
     8401 agttcgtgga gagattcgag aggcccagcg gcgagaagat cgccagctgt gccgccgagc
     8461 tgacctacct gtgctggatg atcacccaca acggcaccgc catcaagagg gccaccttca
     8521 tgagctacaa caccatcatc agcaacagcc tgagcttcga catcgtgaac aagagcctgc
     8581 agttcaagta caagacccag aaggccacca tcctggaggc cagcctgaag aagctgatcc
     8641 ccgcctggga gttcaccatc atcccttaca acggccagaa gcaccagagc gacatcaccg
     8701 acatcgtgtc cagcctgcag ctgcagttcg agagcagcga ggaggccgac aagggcaaca
     8761 gccacagcaa gaagatgctg aaggccctgc tgtccgaggg cgagagcatc tgggagatca
     8821 ccgagaagat cctgaacagc ttcgagtaca ccagcaggtt caccaagacc aagaccctgt
     8881 accagttcct gttcctggcc acattcatca actgcggcag gttcagcgac atcaagaacg
     8941 tggaccccaa gagcttcaag ctggtgcaga acaagtacct gggcgtgatc attcagtgcc
     9001 tggtgaccga gaccaagaca agcgtgtcca ggcacatcta ctttttcagc gccagaggca
     9061 ggatcgaccc cctggtgtac ctggacgagt tcctgaggaa cagcgagccc gtgctgaaga
     9121 gagtgaacag gaccggcaac agcagcagca acaagcagga gtaccagctg ctgaaggaca
     9181 acctggtgcg cagctacaac aaggccctga agaagaacgc cccctacccc atcttcgcta
     9241 tcaagaacgg ccctaagagc cacatcggca ggcacctgat gaccagcttt ctgagcatga
     9301 agggcctgac cgagctgaca aacgtggtgg gcaactggag cgacaagagg gcctccgccg
     9361 tggccaggac cacctacacc caccagatca ccgccatccc cgaccactac ttcgccctgg
     9421 tgtccaggta ctacgcctac gaccccatca gcaaggagat gatcgccctg aaggacgaga
     9481 ccaaccccat cgaggagtgg cagcacatcg agcagctgaa gggcagcgcc gagggcagca
     9541 tcagataccc cgcctggaac ggcatcatca gccaggaggt gctggactac ctgagcagct
     9601 acatcaacag gcggatcacc ggtaactttg accttctcaa gttggccggc gacgtcgagt
     9661 ccaacccagg gcccctgcag caaattttcg tgaagaccct gacgggcaag accatcactc
     9721 ttgaggtcga gcccagtgac accatcgaga atgtcaaggc caagatccaa gacaaggaag
     9781 gcatcccacc tgaccagcag aggctgatat tcgcgggcaa acagctggag gatggccgca
     9841 ccctgtccga ctacaacatc cagaaagagt ccaccttgca cctggtgctg tgtctccgcg
     9901 gtggaatgtc cggtcgtaaa gcccagggca aaaccctagg cgtaaatatg gtccgacgcg
     9961 gagtccgcaa catgacaatg tccatgagca tgatcttggt aggagtgatc atgatgtttt
    10021 tgtctctagg agttggggcg gatcaaggat gcgccatcaa ctttggcaag agagagctcg
    10081 gatcacggtc cgggacacgc gtgtacatgg acgcagtctt tgaatacacc atagactgcg
    10141 atggatctat cttgggtgca gcggtgaacg gaaaaaagag tgcccatggc tctccaacat
    10201 tttggatggg aagtcatgaa gtaaatggga catggatgat ccacaccttg gaggcattag
    10261 attacaagga gtgtgagtgg ccactgacac atacgattgg aacatcagtt gaagagagtg
    10321 aaatgttcat gccgagatca atcggaggcc cagttagctc tcacaatcat atccctggat
    10381 acaaggttca gacgaacgga ccttggatgc aggtaccact agaagtgaag agagaagctt
    10441 gcccagggac tagcgtgatc attgatggca actgtgatgg acggggaaaa tcaaccagat
    10501 ccaccacgga tagcgggaaa gttattcctg aatggtgttg ccgctcctgc acaatgccgc
    10561 ctgtgagctt ccatggtagt gatgggtgtt ggtatcccat ggaaattagg ccaaggaaaa
    10621 cgcatgaaag ccatctggtg cgctcctggg ttacagctgg agaaatacat gctgtccctt
    10681 ttggtttggt gagcatgatg atagcaatgg aagtggtcct aaggaaaaga cagggaccaa
    10741 agcaaatgtt ggttggagga gtagtgctct tgggagcaat gctggtcggg caagtaactc
    10801 tccttgattt gctgaaactc acagtggctg tgggattgca tttccatgag atgaacaatg
    10861 gaggagacgc catgtatatg gcgttgattg ctgccttttc aatcagacca gggctgctca
    10921 tcggctttgg gctcaggacc ctatggagcc ctcgggaacg ccttgtgctg accctaggag
    10981 cagccatggt ggagattgcc ttgggtggcg tgatgggcgg cctgtggaag tatctaaatg
    11041 cagtttctct ctgcatcctg acaataaatg ctgttgcttc taggaaagca tcaaatacca
    11101 tcttgcccct catggctctg ttgacacctg tcactatggc tgaggtgaga cttgccgcaa
    11161 tgttcttttg tgccatggtt atcatagggg tccttcacca gaatttcaag gacacctcca
    11221 tgcagaagac tatacctctg gtggccctca cactcacatc ttacctgggc ttgacacaac
    11281 cttttttggg cctgtgtgca tttctggcaa cccgcatatt tgggcgaagg agtatcccag
    11341 tgaatgaggc actcgcagca gctggtctag tgggagtgct ggcaggactg gcttttcagg
    11401 agatggagaa cttccttggt ccgattgcag ttggaggact cctgatgatg ctggttagcg
    11461 tggctgggag ggtggatggg ctagagctca agaagcttgg tgaagtttca tgggaagagg
    11521 aggcggagat cagcgggagt tccgcccgct atgatgtggc actcagtgaa caaggggagt
    11581 tcaagctgct ttctgaagag aaagtgccat gggaccaggt tgtgatgacc tcgctggcct
    11641 tggttggggc tgccctccat ccatttgctc ttctgctggt ccttgctggg tggctgtttc
    11701 atgtcagggg agcgcgcaga agtggtcacc ggagaagtgg ggatgtcttg tgggatattc
    11761 ccactcctaa gatcatcgag gaatgtgaac atctggagga tgggatttat ggcatattcc
    11821 agtcaacctt cttgggggcc tcccagcgag gagtgggagt ggcacaggga ggggtgttcc
    11881 acacaatgtg gcatgtcaca agaggagctt tccttgtcag gaatggcaag aagttgattc
    11941 catcttgggc ttcagtaaag gaagaccttg tcgcctatgg tggctcatgg aagttggaag
    12001 gcagatggga tggagaggaa gaggtccagt tgatcgcggc tgttccagga aagaacgtgg
    12061 tcaacgtcca gacaaaaccg agcttgttca aagtgaggaa tgggggagaa atcggggctg
    12121 tcgctcttga ctatccgagt ggcacttcag gatctcctat tgttaacagg aacggagagg
    12181 tgattgggct gtacggcaat ggcatccttg tcggtgacaa ctccttcgtg tccgccatat
    12241 cccagactga ggtgaaggaa gaaggaaagg aggagctcca agagatcccg acaatgctaa
    12301 agaaaggaat gacaactgtc cttgattttc atcctggagc tgggaagaca agacgtttcc
    12361 tcccacagat cttggccgag tgcgcacgga gacgcttgcg cactcttgtg ttggccccca
    12421 ccagggttgt tctttctgaa atgaaggagg cttttcacgg cctggacgtg aaattccaca
    12481 cacaggcttt ttccgctcac ggcagcggga gagaagtcat tgatgccatg tgccatgcca
    12541 ccctaactta caggatgttg gaaccaacta gggttgttaa ctgggaagtg atcattatgg
    12601 atgaagccca ttttttggat ccagctagca tagccgctag aggttgggca gcgcacagag
    12661 ctagggcaaa tgaaagtgca acaatcttga tgacagccac accgcctggg actagtgatg
    12721 aatttccaca ttcaaatggt gaaatagaag atgttcaaac ggacataccc agtgagccct
    12781 ggaacacagg gcatgactgg atcctggctg acaaaaggcc cacggcatgg ttccttccat
    12841 ccatcagagc tgcaaatgtc atggctgcct ctttgcgtaa ggctggaaag agtgtggtgg
    12901 tcctgaacag gaaaaccttt gagagagaat accccacgat aaagcagaag aaacctgact
    12961 ttatattggc cactgacata gctgaaatgg gagccaacct ttgcgtggag cgagtgctgg
    13021 attgcaggac ggcttttaag cctgtgcttg tggatgaagg gaggaaggtg gcaataaaag
    13081 ggccacttcg tatctccgca tcctctgctg ctcaaaggag ggggcgcatt gggagaaatc
    13141 ccaacagaga tggagactca tactactatt ctgagcctac aagtgaaaat aatgcccacc
    13201 acgtctgctg gttggaggcc tcaatgctct tggacaacat ggaggtgagg ggtggaatgg
    13261 tcgccccact ctatggcgtt gaaggaacta aaacaccagt ttcccctggt gaaatgagac
    13321 tgagggatga ccagaggaaa gtcttcagag aactagtgag gaattgtgac ctgcccgttt
    13381 ggctttcgtg gcaagtggcc aaggctggtt tgaagacgaa tgatcgtaag tggtgttttg
    13441 aaggccctga ggaacatgag atcttgaatg acagcggtga aacagtgaag tgcagggctc
    13501 ctggaggagc aaagaagcct ctgcgcccaa ggtggtgtga tgaaagggtg tcatctgacc
    13561 agagtgcgct gtctgaattt attaagtttg ctgaaggtag gaggggagct gctgaagtgc
    13621 tagttgtgct gagtgaactc cctgatttcc tggctaaaaa aggtggagag gcaatggata
    13681 ccatcagtgt gttcctccac tctgaggaag gctctagggc ttaccgcaat gcactatcaa
    13741 tgatgcctga ggcaatgaca atagtcatgc tgtttatact ggctggacta ctgacatcgg
    13801 gaatggtcat ctttttcatg tctcccaaag gcatcagtag aatgtctatg gcgatgggca
    13861 caatggccgg ctgtggatat ctcatgttcc ttggaggcgt caaacccact cacatctcct
    13921 atgtcatgct catattcttt gtcctgatgg tggttgtgat ccccgagcca gggcaacaaa
    13981 ggtccatcca agacaaccaa gtggcatacc tcattattgg catcctgacg ctggtttcag
    14041 cggtggcagc caacgagcta ggcatgctgg agaaaaccaa agaggacctc tttgggaaga
    14101 agaacttaat tccatctagt gcttcaccct ggagttggcc ggatcttgac ctgaagccag
    14161 gagctgcctg gacagtgtac gttggcattg ttacaatgct ctctccaatg ttgcaccact
    14221 ggatcaaagt cgaatatggc aacctgtctc tgtctggaat agcccagtca gcctcagtcc
    14281 tttctttcat ggacaagggg ataccattca tgaagatgaa tatctcggtc ataatgctgc
    14341 tggtcagtgg ctggaattca ataacagtga tgcctctgct ctgtggcata gggtgcgcca
    14401 tgctccactg gtctctcatt ttacctggaa tcaaagcgca gcagtcaaag cttgcacaga
    14461 gaagggtgtt ccatggcgtt gccaagaacc ctgtggttga tgggaatcca acagttgaca
    14521 ttgaggaagc tcctgaaatg cctgcccttt atgagaagaa actggctcta tatctccttc
    14581 ttgctctcag cctagcttct gttgccatgt gcagaacgcc cttttcattg gctgaaggca
    14641 ttgtcctagc atcagctgcc ttagggccgc tcatagaggg aaacaccagc cttctttgga
    14701 atggacccat ggctgtctcc atgacaggag tcatgagggg gaatcactat gcttttgtgg
    14761 gagtcatgta caatctatgg aagatgaaaa ctggacgccg ggggagcgcg aatggaaaaa
    14821 ctttgggtga agtctggaag agggaactga atctgttgga caagcgacag tttgagttgt
    14881 ataaaaggac cgacattgtg gaggtggatc gtgatacggc acgcaggcat ttggccgaag
    14941 ggaaggtgga caccggggtg gcggtctcca gggggaccgc aaagttaagg tggttccatg
    15001 agcgtggcta tgtcaagctg gaaggtaggg tgattgacct ggggtgtggc cgcggaggct
    15061 ggtgttacta cgctgctgcg caaaaggaag tgagtggggt caaaggattt actcttggaa
    15121 gagacggcca tgagaaaccc atgaatgtgc aaagtctggg atggaacatc atcaccttca
    15181 aggacaaaac tgatatccac cgcctagaac cagtgaaatg tgacaccctt ttgtgtgaca
    15241 ttggagagtc atcatcgtca tcggtcacag agggggaaag gaccgtgaga gttcttgata
    15301 ctgtagaaaa atggctggct tgtggggttg acaacttctg tgtgaaggtg ttagctccat
    15361 acatgccaga tgttcttgag aaactggaat tgctccaaag gaggtttggc ggaacagtga
    15421 tcaggaaccc tctctccagg aattccactc atgaaatgta ctacgtgtct ggagcccgca
    15481 gcaatgtcac atttactgtg aaccaaacat cccgcctcct gatgaggaga atgaggcgtc
    15541 caactggaaa agtgaccctg gaggctgacg tcatcctccc aattgggaca cgcagtgttg
    15601 agacagacaa gggacccctg gacaaagagg ccatagaaga aagggttgag aggataaaat
    15661 ctgagtacat gacctcttgg ttttatgaca atgacaaccc ctacaggacc tggcactact
    15721 gtggctccta tgtcacaaaa acctcaggaa gtgcggcgag catggtaaat ggtgttatta
    15781 aaattctgac atatccatgg gacaggatag aggaggtcac aagaatggca atgactgaca
    15841 caaccccttt tggacagcaa agagtgttta aagaaaaagt tgacaccaga gcaaaggatc
    15901 caccagcggg aactaggaag atcatgaaag ttgtcaacag gtggctgttc cgccacctgg
    15961 ccagagaaaa gaaccccaga ctgtgcacaa aggaagaatt tattgcaaaa gtccgaagtc
    16021 atgcagccat tggagcttac ctggaagaac aagaacagtg gaagactgcc aatgaggctg
    16081 tccaagaccc aaagttctgg gaactggtgg atgaagaaag gaagctgcac caacaaggca
    16141 ggtgtcggac ttgtgtgtac aacatgatgg ggaaaagaga gaagaagctg tcagagtttg
    16201 ggaaagcaaa gggaagccgt gccatatggt atatgtggct gggagcgcgg tatcttgagt
    16261 ttgaggccct gggattcctg aatgaggacc attgggcttc cagggaaaac tcaggaggag
    16321 gagtggaagg cattggctta caatacctag gatatgtgat cagagacctg gctgcaatgg
    16381 atggtggtgg attctacgcg gatgacaccg ctggatggga cacgcgcatc acagaggcag
    16441 accttgatga tgaacaggag atcttgaact acatgagccc acatcacaaa aaactggcac
    16501 aagcagtgat ggaaatgaca tacaagaaca aagtggtgaa agtgttgaga ccagccccag
    16561 gagggaaagc ctacatggat gtcataagtc gacgagacca gagaggatcc gggcaggtag
    16621 tgacttatgc tctgaacacc atcaccaact tgaaagtcca attgatcaga atggcagaag
    16681 cagagatggt gatacatcac caacatgttc aagattgtga tgaatcagtt ctgaccaggc
    16741 tggaggcatg gctcactgag cacggatgtg acagactgaa gaggatggcg gtgagtggag
    16801 acgactgtgt ggtccggccc atcgatgaca ggttcggcct ggccctgtcc catctcaacg
    16861 ccatgtccaa ggttagaaag gacatatctg aatggcagcc atcaaaaggg tggaatgatt
    16921 gggagaatgt gcccttctgt tcccaccact tccatgaact acagctgaag gatggcagga
    16981 ggattgtggt gccttgccga gaacaggacg agctcattgg gagaggaagg gtgtctccag
    17041 gaaacggctg gatgatcaag gaaacagctt gcctcagcaa agcctatgcc aacatgtggt
    17101 cactgatgta ttttcacaaa agggacatga ggctactgtc attggctgtt tcctcagctg
    17161 ttcccacctc atgggttcca caaggacgca caacatggtc gattcatggg aaaggggagt
    17221 ggatgaccac ggaagacatg cttgaggtgt ggaacagagt atggataacc aacaacccac
    17281 acatgcagga caagacaatg gtgaaaaaat ggagagatgt cccttatcta accaagagac
    17341 aagacaagct gtgcggatca ctgattggaa tgaccaatag ggccacctgg gcctcccaca
    17401 tccatttagt catccatcgt atccgaacgc tgattggaca ggagaaatac actgactacc
    17461 taacagtcat ggacaggtat tctgtggatg ctgacctgca actgggtgag cttatctgaa
    17521 acaccatcta acaggaataa ccgggataca aaccacgggt ggagaaccgg actccccaca
    17581 acctgaaacc gggatataaa ccacggctgg agaaccggac tccgcactta aaatgaaaca
    17641 gaaaccggga taaaaactac ggatggagaa ccggactcca cacattgaga cagaagaagt
    17701 tgtcagccca gaaccccaca cgagttttgc cactgctaag ctgtgaggca gtgcaggctg
    17761 ggacagccga cctccaggtt gcgaaaaacc tggtttctgg gacctcccac cccagagtaa
    17821 aaagaacgga gcctccgcta ccaccctccc acgtggtggt agaaagacgg ggtctagagg
    17881 ttagaggaga ccctccaggg aacaaatagt gggaccatat tgacgccagg gaaagaccgg
    17941 agtggttctc tgcttttcct ccagaggtct gtgagcacag tttgctcaag aataagcaga
    18001 cctttggatg acaaacacaa aaccactcga gcaagacgtt tcccgttgaa tatggctcat
    18061 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
    18121 tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa
    18181 tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg cagaccgttc
    18241 cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag ctctcatcaa
    18301 ccgtggctcc ctcactttct ggctggatga tggggcgatt caggcctggt atgagtcagc
    18361 aacaccttct tcacgaggca gacctcagcg ctagcggagt gtagtgggat ccccgggtac
    18421 cgagctcgaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
    18481 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
    18541 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
    18601 gctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
    18661 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
    18721 ccgacacccg ccaacacccg ctgacgcgaa ccccttgcgg ccg
//
