LOCUS       sequence-467474-       13555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 (–)-Eldh2 without accessory genes 
            (inactive) in Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13555)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 13555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80EN1-C1858_02810-(–)-Eldh2-noAccs (ElA2)
FEATURES             Location/Qualifiers
     source          1..13555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      7179..7206
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      7338..7365
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(7797..8648)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(8635..9474)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(9985..10956)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            12195..12282
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      12624..13018
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(13181..13224)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            13320..13429
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctac
     1501 tgctcgcttt tgcgatcctt acgccggtcg agaagagttt gggcctcttc tgccgcagcg
     1561 aagaaatcgc gtaagcgttc ttcgtttcta ggatggtaga cggcgtatgc tacgaggttc
     1621 ttgtcttttt cctcgatcgg tataccggtg cgctttccgg tttccagcag taagccgatc
     1681 tgcttttgcg tctttttcca tatgaggacg ttgcctctga gcggtgtgct gaagaactcc
     1741 acgttgttgc ctgccgatac gggcctggtc ttgggctcga atccgtaatc gatacatgcg
     1801 gcttcgattt gatcccaggc agggttcgtc ttgtcgctca tgaatttcac cagcgtttgc
     1861 gtttgcagat ctttccacga gatcgaggtt cggttggcaa gcggatggtc gttttctacg
     1921 attgcgagca attgaatcga cacgaacggc ctactgatca atccctcttg ttcggcgtga
     1981 cgggggttgg tgtatccgat gaacaaatcg atggactctc tcgatagcaa cgtgaagggg
     2041 tcgtcgatgt tgcggtcgaa aacgatgttc gcgtgatgat tttcgcgggc gatcattgcc
     2101 gtcatagaag cgagggaggc tatgtcggag tcttccatgt gtccgaccac gtgaataggg
     2161 cgcttcgctt tgagctgttc gagcgtgttt ctggtcttgt cgtattcgtt cacgatgacc
     2221 ccggcgcttt cgaacaagat gcgtccgcct tcggttagct gaactccttt tttccctcga
     2281 tcaagcagtt ctattccgaa ttctttttca agggcgagca tgtgcttgct caatgccggt
     2341 tgggtgatgt tgaggtttcc ggccgcctcg gtgaagttca gacattgagc gagctcgatg
     2401 aattctctgc aggattcgat gttcatgatg accccccccc tccgccgaat gaaaacccat
     2461 tcggcgattg cattgcgagg ttcgatgccc ccccagcatc gctcgtctta aaagccattg
     2521 tagcatcctg ggtgattttg agaggtacac gcgctttcct taggcctgtt ggcatggtct
     2581 tataacgcat cggaatggga ggggcttgaa tcggaatgca tgcggggcga tgtgtgccga
     2641 aacgttattt ccctatcttc gagttgcttt tcatactgaa ggaagatgcg atctccttaa
     2701 tggggtcgtc gtgggcaggg tcgaaaataa ggaggagggt atgggtaaac tgcatatgtc
     2761 gcgacgggat ttcgtcaaag tctcggtcgt gacaggcgcg gcgttcggca tctccgcgtt
     2821 ggctgagccg agtttggctg ttgctgacga tgccgtcgat aagccggtgg gaggtgacat
     2881 caagcgtatc cgaacagggt gtcgcggctg cggcaaaatg gaatgcggcg ttatcgccgt
     2941 agtgcagaac gggcgtgtcg ttcggatgga gggcgaccct acggcgttcc agtcgatggg
     3001 gaactgctgc accaaatcgc aagcggccat tcaggcggca taccatcctg atcgtctgca
     3061 ttatcctatg aaacgaacga acgacaagga atccgatgat cccggttggg tgcgtatttc
     3121 ttgggatgag gccatgacca cgatcgtgtc gaaattcgac gagctgcaag cgcgctacgg
     3181 aggcgaatcg ctgttcggca tgtgcggcac gtcgcgcgtg tggtgcatgt tcggagcgtc
     3241 caacggcatg tacctgtggg attcgccgaa catcgtgcag gcgtggcaga tctgcaaggg
     3301 cccgcgccat ttcgccactc ttatgtgctc gagcttcgcc gacagctgga tggagacggt
     3361 tgcccatcct gatgtgtacg tggcctgggg cggtgcatcc gagatgtcga actacgatga
     3421 cagctgccgc accactgtgg atgtggcgac gcgctcgacc acgcacattt gcgtcgatcc
     3481 gcgcgaaacg aacatgggta aagaggcgga cttccagctg cacctgcgac ccggtaccga
     3541 tggggcgatg gcgttggctt ggacgaacgt catcattgaa aacaatctgt acgacgagtt
     3601 gttcacgaag aagtggacca acggtccgtt tctcgtgtgc gaagacatgg agccgaccgg
     3661 ctggaccgag gcttcgccca ttgtcatgac cccattcgaa ctcaagacgc atttgctgaa
     3721 ggaatcggat ctgctggagg acggaagccc aaagcgtttt atggtttggg acaaccttgc
     3781 tgggaggctg acctatttcg attccgaaac cggtttttgg gaaggtgagg aatggacgaa
     3841 gccgacggcc gggaaggaag cttcgcagga gaatctcgcg cctggcttcc atcaaggttt
     3901 tgtcattgat cctactccgt tcgaccctga aatagatccg gcgttgtttg gggaattcga
     3961 agtgacgctc aaagacgggt cgacgcataa ggtgcagccg gtgtttcaaa agtacgccga
     4021 tcgctgcgcg gaatacactc ctgaagtcgc tgaggaaatc accggggttc cggccgatca
     4081 gattcgtgcg gcggctatgg cttacggcac gccccttcat ccggaaaagg gttatggcaa
     4141 tggcggtatt cagtacatgc tggccaccga gcatgcgaat accgccatcc agaatgtgcg
     4201 cgcgcttgat tatctgacgg ccatcacggg aaattacgat acgccgggcg gtcagcgtgc
     4261 gtcaacgcgc gctcccatcg agggcggtca gatgggtttt gccaacaacg gttcgggcat
     4321 gcccatgctt tcgccggggc agatggagaa gatcttaggt cgcgatgaga ttccgctgct
     4381 gtattggtgg ggcatgtggg ccgatgctgc ggctacgtgg aatgcggtgc tcaccggaga
     4441 accgtatccg gtcgttggcg cgttcaattc gtcggggaac ttcatgaacc agtgcaacac
     4501 caccacgaca tgggaggctc tcaagaagct cgatttttat gtggaatgca acctgtggca
     4561 cactcctggc ggtggtttgt gcgacatcgt tttgcctgcc gctcatttct tggagttgtc
     4621 cagtccgcgt tcatcccagg gcgcttcggg cgctatgggc gcgacggtca aatgcatcga
     4681 cccgcctggt gaagcgaagt tcgacggcga gatcattcgc atgttgtacg aatacaaagg
     4741 cctcccctat aacgttattc ctggttatcc tgaatatcct agcgtcgagg aaatgctgga
     4801 catgtcggtg gcgggtttcg agccgaaggg atggaaggta ttcgagcagc gtttccagga
     4861 acatggctgg tgggattgca aggaaactga accggaattg tggggaacgt atcgccgcta
     4921 cgaaaccggt gcgaatcgcg ctcgcaatgc gggtgggttc gtaggttctc aaggtgattg
     4981 gcttcccggg ttctacactc cgacaaagaa gatcgaaata tggtctacgg tcatggagtc
     5041 gttccatccc gatggcgagt ggaatcttcc cacgtatcgc gaaccaccgc acagtcctgt
     5101 gagcgatcct gacatgtaca aagaataccc gctgatcatc actacgggac gccgcattcc
     5161 cgtgtacttc cattccgagc accggcagct gccgtggtgt cgcgagcaat ggccggtgcc
     5221 gcgcgtggaa atcaatccag acgatgccgc cacgtatggc atagaacagg gagattgggt
     5281 ttggattgag acgccatatg ggaagattcg cgaggttgcc gatttgtact acgggatccg
     5341 tcccggcact atcaattgcg agcatcagtg gtggttcccg gaattcaagc aatcgggatc
     5401 gggccacgag ctatgcgcgg tcaaccactt gatagacaac tgggcgcgcg atccgcattg
     5461 cggcgcgtct aacctgcgtg cgtacctcgg caagatatac aaggcaacgc ccgagaactc
     5521 cccgttcggc aatccggtgc cttgcgacca caacggcgtt gagatcatcc acgatgcttc
     5581 cgatcctcgt ctcaaagaat ggctgccgac gtacgaaggg agggagtagg ccatgaccca
     5641 gtatgctatt gtcaccgact tgaatcggtg cgtcgggtgc ttggcatgcg ctgtcgcatg
     5701 caaagccgtg aacgatgtgc ccattggcag tttttggaac aaggttttac gcatcgggcc
     5761 gaatgcttct atacaaggtg cgcaggatgc ggattcttat ctttacttcc tgcccgtgca
     5821 gtgccagcac tgcgccgacc ccgagtgcgt gaaggtgtgc ccgacgggcg cctcgcacaa
     5881 gcttgaggac ggcacggtgc aggttgacaa gtccaagtgc atcggctgcc agttctgcgc
     5941 gatggcttgt ccctacaacg ttcgttactt gaacgagcag gagcgcgtgg ttgagaagtg
     6001 caccctttgc gagcagaaga tcgcccaggg cgagctgccg caatgcgtca gccaatgcgg
     6061 tggcatggcg cgcttcttcg gtgatttgga taagggcatc gaatcgttcg aaggtgcgga
     6121 aaacggaacc gagcgcattg ttctcgggga gttctgcaag cccttcacgg atgaagacgt
     6181 gcacgtgctg cctaatgtgg gaaacaatcc gtcggtgcgc tatatcctgc gtgaccatga
     6241 atggcaagga ggcggtgaat aatggagctc cagtggcctt tgatcgtgtt caccacgctg
     6301 gtggcgtgga gcgcggggct gttcgggacg caggcgctga tggccgcgtt cggcgtcggg
     6361 aagaaggcgc aggtcccggc ctggatcgcg agcgccgtcc ttctggccgt cggcgggatc
     6421 gcggtgttct tccacctcga gcactgggag cgcatcttca acggcttcgg ccaccttacg
     6481 agcggcatca cccaggagct gatcgccatc gtcgtgctgg cggccgtcgc cgtggcctac
     6541 ctggtcatgc tgcgcaagtc cgacgacggc gcgagcgtgc cgaagtggct ggcgtggctg
     6601 tcggtcgcgc tgtcggtcgc gctggtggcc gtgatggcgc actcgtacac gatggccgcg
     6661 aggccggcgt gggatagcgt gctgtggatc ctctacgtcc tgggcaacgc ctgcgtgctg
     6721 ggaccggcga cgttcgcgtt cctcatggcc gcagcgccgc ccgcaggtca gcccgccggg
     6781 cgcgcggccg acgccggggc tcccgcaggc ctcgctgccc tcgtaggcgc cgccgcgaac
     6841 gccctggccg ccctcgcctt cgcgatcttc ctccagctct ccgccggctc cttcgccgac
     6901 gtcggctact acttcgaccc gacgcatccg accgaggcca tggccgacgc cgcggcgacg
     6961 gtcggcgcgc aagcgcccct gctgtggctc ggcgcggtcg ccgtcggcgc tatcgtcccg
     7021 ctcgcggcgg ccttcatggg caggcgctcc ggcaactgga ggctctgggc cccggtggct
     7081 atcatcgccg ccctggcggg cgccgtctgc atgcgcgtgg ccttctacaa cctcggcctc
     7141 agcgtgttca tgttctacta gactagtcga ctcacactgg ctcaccttcg ggtgggcctt
     7201 tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta
     7261 ttatttaatt cctgttataa aaaaaggatc aattttgaac tctctcccaa agttgatccc
     7321 ttaacgatcc ctgcaggaga aggccatcct gacggatggc ctttttgaag ctttctagaa
     7381 tcgggggccc ccggggggat tctagaacaa tggaagtcct cgtctcgccc tcgaattagc
     7441 ccgcttaatg agcgggcttt ttttgaatta attctcgcga gctggcacga caggtttccc
     7501 gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagcgcga attgcaagct
     7561 ggccgacgcg ctgggctacg tcttgctggc gttcgggagc agaagagcat acatctggaa
     7621 gcaaagccag gaaagcggcc tatggagctg tgcggcagcg ctcagtaggc aatttttcaa
     7681 aatattgtta agccttttct gagcatggta tttttcatgg tattaccaat tagcaggaaa
     7741 ataagccatt gaatataaaa gataaaaatg tcttgtttac aatagagtgg ggggggtcag
     7801 cctgccgcct tgggccgggt gatgtcgtac ttgcccgccg cgaactcggt taccgtccag
     7861 cccagcgcga ccagctccgg caacgcctcg cgcacccgct ggcggcgctt gcgcatggtc
     7921 gaaccactgg cctctgacgg ccagacatag ccgcacaagg tatctatgga agccttgccg
     7981 gttttgccgg ggtcgatcca gccacacagc cgctggtgca gcaggcgggc ggtttcgctg
     8041 tccagcgccc gcacctcgtc catgctgatg cgcacatgct ggccgccacc catgacggcc
     8101 tgcgcgatca aggggttcag ggccacgtac aggcgcccgt ccgcctcgtc gctggcgtac
     8161 tccgacagca gccgaaaccc ctgccgcttg cggccattct gggcgatgat ggataccttc
     8221 caaaggcgct cgatgcagtc ctgtatgtgc ttgagcgccc caccactatc gacctctgcc
     8281 ccgatttcct ttgccagcgc ccgatagcta cctttgacca ccatggcatc agcggtgacg
     8341 gcctcccact tgggttccag gaacagccgg agctgccgtc cgccttcggt cttgggttcc
     8401 gggccaagca ctaggccatt aggcccagcc atggccacca gcccttgcag gatgcgcaga
     8461 tcatcagcgc ccagcggctc cgggccgctg aactcgatcc gcttgccgtc gccgtagtca
     8521 tacgtcacgt ccagcttgct gcgcttgcgc tcgccccgct tgagggcacg gaacaggccg
     8581 ggggccagac agtgcgccgg gtcgtgtcgg acgtggctga ggctgtgctt gttcttaggc
     8641 ttcaccacgg ggcaccccct tgctcttgcg ctgcctctcc agcacggcgg gcttgagcac
     8701 cccgccgtca tgccgcctga accaccgatc agcgaacggt gcgccatagt tggccttgct
     8761 cacaccgaag cggacgaaga accggcgctg gtcgtcgtcc acaccccatt cctcggcctc
     8821 ggcgctggtc atgctcgaca ggtaggactg ccagcggatg ttatcgacca gtaccgagct
     8881 gccccggctg gcctgctgct ggtcgcctgc gcccatcatg gccgcgccct tgctggcatg
     8941 gtgcaggaac acgatagagc acccggtatc ggcggcgatg gcctccatgc gaccgatgac
     9001 ctgggccatg gggccgctgg cgttttcttc ctcgatgtgg aaccggcgca gcgtgtccag
     9061 caccatcagg cggcggccct cggcggcgcg cttgaggccg tcgaaccact ccggggccat
     9121 gatgttgggc aggctgccga tcagcggctg gatcagcagg ccgtcagcca cggcttgccg
     9181 ttcctcggcg ctgaggtgcg ccccaagggc gtgcaggcgg tgatgaatgg cggtgggcgg
     9241 gtcttcggcg ggcaggtaga tcaccgggcc ggtgggcagt tcgcccacct ccagcagatc
     9301 cggcccgcct gcaatctgtg cggccagttg cagggccagc atggatttac cggcaccacc
     9361 gggcgacacc agcgccccga ccgtaccggc caccatgttg ggcaaaacgt agtccagcgg
     9421 tggcggcgct gctgcgaacg cctccagaat attgataggc ttatgggtag ccattgattg
     9481 cctcctttgc aggcagttgg tggttaggcg ctggcggggt cactaccccc gccctgcgcc
     9541 gctctgagtt cttccaggca ctcgcgcagc gcctcgtatt cgtcgtcggt cagccagaac
     9601 ttgcgctgac gcatcccttt ggccttcatg cgctcggcat atcgcgcttg gcgtacagcg
     9661 tcagggctgg ccagcaggtc gccggtctgc ttgtcctttt ggtctttcat atcagtcacc
     9721 gagaaacttg ccggggccga aaggcttgtc ttcgcggaac aaggacaagg tgcagccgtc
     9781 aaggttaagg ctggccatat cagcgactga aaagcggcca gcctcggcct tgtttgacgt
     9841 ataaccaaag ccaccgggca accaatagcc cttgtcactt ttgatcaggt agaccgaccc
     9901 tgaagcgctt ttttcgtatt ccataaaacc cccttctgtg cgtgagtact catagtataa
     9961 caggcgtgag taccaacgca agcactacat gctgaaatct ggcccgcccc tgtccatgcc
    10021 tcgctggcgg ggtgccggtg cccgtgccag ctcggcccgc gcaagctgga cgctgggcag
    10081 acccatgacc ttgctgacgg tgcgctcgat gtaatccgct tcgtggccgg gcttgcgctc
    10141 tgccagcgct gggctggcct cggccatggc cttgccgatt tcctcggcac tgcggccccg
    10201 gctggccagc ttctgcgcgg cgataaagtc gcacttgctg aggtcatcac cgaagcgctt
    10261 gaccagcccg gccatctcgc tgcggtactc gtccagcgcc gtgcgccggt ggcggctaag
    10321 ctgccgctcg gacagttcga ggctggccag cctgcgggcc ttctcctgct gccgctgggc
    10381 ctgctcgatc tgctggccag cctgctgcac cagcgccggg ccagcggtgg cggtcttgcc
    10441 cttggattca cgcagcagca cccacggctg ataaccggcg cgggtggtgt gcttgtcctt
    10501 gcggttggtg aagcccgcca agcggccata gtggcggctg tcggcgctgg ccgggtcggc
    10561 gtcgtactcg ctggccagcg tccgggcaat ctgcccccga agttcaccgc ctgcggcgtc
    10621 ggccaccttg acccatgcct gatagttctt cgggctggtt tccactacca gggcaggctc
    10681 ccggccctcg gctttcatgt catccaggtc aaactcgctg aggtcgtcca ccagcaccag
    10741 accatgccgc tcctgctcgg cgggcctgat atacacgtca ttgccctggg cattcatccg
    10801 cttgagccat ggcgtgttct ggagcacttc ggcggctgac cattcccggt tcatcatctg
    10861 gccggtggtg gcgtccctga cgccgatatc gaagcgctca cagcccatgg ccttgagctg
    10921 tcggcctatg gcctgcaaag tcctgtcgtt cttcatcggg ccaccaagcg cagccagatc
    10981 gagccgtcct cggttgtcag tggcgtcagg tcgagcaaga gcaacgatgc gatcagcagc
    11041 accaccgtag gcatcatgga agccagcatc acggttagcc atagcttcca gtgccacccc
    11101 cgcgacgcgc tccgggcgct ctgcgcggcg ctgctcacct cggcggctac ctcccgcaac
    11161 tctttggcca gctccaccca tgccgcccct gtctggcgct gggctttcag ccactccgcc
    11221 gcctgcgcct cgctggcctg ctgggtctgg ctcatgacct gccgggcttc gtcggccagt
    11281 gtcgccatgc tctgggccag cggttcgatc tgctccgcta actcgttgat gcctctggat
    11341 ttcttcactc tgtcgattgc gttcatggtc tattgcctcc cggtattcct gtaagtcgat
    11401 gatctgggcg ttggcggtgt cgatgttcag ggccacgtct gcccggtcgg tgcggatgcc
    11461 ccggccttcc atctccacca cgttcggccc caggtgaaca ccgggcaggc gctcgatgcc
    11521 ctgcgcctca agtgttctgt ggtcaatgcg ggcgtcgtgg cctgcccgct ctaatgcccg
    11581 gttggcatgg tcggcccatg cctcgcgggt ctgctcaagc catgccttgg gcttgagcgc
    11641 ttcggtcttc tgtgccccgc ccttctccgg ggtcttgccg ttgtaccgct tgaaccactg
    11701 agcggcgggc cgctcgatgc cgtcattgat ccgctcggag atcatcaggt ggcagtgcgg
    11761 gttctcgccg ccaccggcat ggatggccag cgtatacggc aggcgctcgg caccggtcag
    11821 gtgctgggcg aactcggacg ccagcgcctt ctgctggtcg agggtcagct cgaccggcag
    11881 ggcaaattcg acctccttga acagccgccc attggcgcgt tcatacaggt cggcagcatc
    11941 ccagtagtcg gcgggccgct cgacgaactc cggcatgtgc ccggattcgg cgtgcaagac
    12001 ttcatccatg tcgcgggcat acttgccttc gcgctggatg aagtcggcct tggccctggc
    12061 cgattggccg cccgacctgc tgccggtttt cgccgtaagg tgataaatcg ccatgctgcc
    12121 tcgctgttgc ttttgctttt cggctccatg caatggccct cggagagcgc accgcccgaa
    12181 gggtggccgt taggccagtt tctcgaagag aaaccggtaa gtgcgccctc ccctacaaag
    12241 tagggtcggg attgccgccg ctgtgcctcc atgatagcct acgagacagc acattaacaa
    12301 tggggtgtca agatggttaa ggggagcaac aaggcggcgg atcggctggc caagctcgaa
    12361 gaacaacgag cgcgaatcaa tgccgaaatt cagcgggtgc gggcaaggga acagcagcaa
    12421 gagcgcaaga acgaaacaag gcgcaaggtg ctggtggggg ccatgatttt ggccaaggtg
    12481 aacagcagcg agtggccgga ggatcggctc atggcggcaa tggatgcgta ccttgaacgc
    12541 gaccacgacc gcgccttgtt cggtctgccg ccacgccaga aggatgagcc gggctgaatg
    12601 atcgaccgag acaggccctg cggggctgca cacgcgcccc cacccttcgg gtagggggaa
    12661 aggccgctaa agcggctaaa agcgctccag cgtatttctg cggggtttgg tgtggggttt
    12721 agcgggcttt gcccgccttt ccccctgccg cgcagcggtg gggcggtgtg tagcctagcg
    12781 cagcgaatag accagctatc cggcctctgg ccgggcatat tgggcaaggg cagcagcgcc
    12841 ccacaagggc gctgataacc gcgcctagtg gattattctt agataatcat ggatggattt
    12901 ttccaacacc ccgccagccc ccgcccctgc tgggtttgca ggtttggggg cgtgacagtt
    12961 attgcagggg ttcgtgacag ttattgcagg ggggcgtgac agttattgca ggggttcgtg
    13021 acagttagta cgggagtgac gggcactggc tggcaatgtc tagcaacggc aggcatttcg
    13081 gctgagggta aaagaacttt ccgctaagcg atagactgta tgtaaacaca gtattgcaag
    13141 gacgcggaac atgcctcatg tggcggccct cgcgagaatt aattcagata aaaaaaatcc
    13201 ttagctttcg ctaaggatga tttctagcga tgaccctgct gattggttcg ctgaccgtag
    13261 actggttggc ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc
    13321 cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt
    13381 gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg
    13441 ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgac
    13501 atcgcgcgcg cgcgcgcgga tgctagcggc gcgccaaaaa aaagcccgct catta
//
