LOCUS       sequence-467479-        9644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EF1a-driven LIDAR reporter RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 9644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: YX022 (PB)EF1a-LIDARsensor (hygR)
FEATURES             Location/Qualifiers
     source          1..9644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..487
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          488..1426
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1383..1403
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1442..1451
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1448..2155
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1593..1611)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1861..1880
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2201..2266
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             2435..2500
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(2507..2509,2510..3220)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             3242..3271
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3327..3344)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3333..3540
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3573..3593)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3578..3907
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3758..3893
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3820..3839
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3956..4981
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3956..4981
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(5041..5061)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5103..5224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5140..5159)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5194..5213
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5987..6021
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6512..6528)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6512..6528)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6525..6547)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6536..6552
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6560..6566,6567..6584,6585..6590))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6605..6626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6743..6760)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6914..7502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6994..7013)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7673..8464,8465..8533))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8296..8315
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8534..8638)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8664..9119)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8801..8822)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9013..9032
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9246..9268
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9260..9277
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9261..9277
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   9407..9469
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg
       61 gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca tcccagagcc
      121 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa
      181 cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa aaggcctcca
      241 cggccacaat tgcatgaaga atcgtgcccg tcagtgggca gagcgcacat cgcccacagt
      301 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
      361 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      421 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
      481 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
      541 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
      601 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
      661 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
      721 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
      781 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
      841 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
      901 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
      961 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     1021 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
     1081 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     1141 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     1201 gtccaggcac ctcgattagt tctcgcgctt ttggagtacg tcgtctttag gttgggggga
     1261 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     1321 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     1381 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagctt
     1441 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     1501 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     1561 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     1621 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     1681 gaagcacccc gccgacattc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     1741 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     1801 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     1861 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     1921 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     1981 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     2041 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     2101 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagagcga
     2161 cgcgttggaa gccagacaca aacagaaaat tgtggctccg gtgaaacaga ctttgaattt
     2221 tgaccttctg aagctggcgg gagacgtcga gtccaacccc ggtcccggta ccggatcctc
     2281 cgtttaggtg ggtggaatag taacgtacac catcagggta cgtctcagac accatcaggg
     2341 tctgtctggt acagcatcag cgtaccatag tatcccacct acccagacga ccggtactag
     2401 tgaggcacgg cataagcaaa agatcgtagc cccagtaaag caaacactca acttcgatct
     2461 actcaaactc gcaggtgatg tggaatctaa tccaggaccc tcgagtgtct caaagggcga
     2521 ggaacttttt acgggcgttg ttccgatcct cgtcgaattg gacggggacg ttaatgggca
     2581 taaattctca gttagtggtg agggtgaggg ggacgcgaca tatgggaaac ttacgctgaa
     2641 gttcatatgt accaccggaa aattgccagt gccgtggcct actctcgtca ccaccctgac
     2701 atatggtgtt cagtgtttta gccggtatcc agaccatatg aaacagcacg atttttttaa
     2761 atccgctatg cctgaaggtt acgtacagga gcgaacgata ttttttaaag acgacggaaa
     2821 ctacaaaact agagccgagg taaaattcga gggtgacact ctcgtgaacc ggatcgaact
     2881 gaagggcata gattttaagg aggatggcaa tatactgggc cacaagctcg aatacaacta
     2941 taattctcat aatgtctata tcatggcgga caaacagaaa aacggcatca aggtaaactt
     3001 taaaatccga cacaatattg aggacggctc tgtccagctt gcggaccatt accaacagaa
     3061 cacgccgata ggggatggtc cagtacttct ccctgataat cactatttgt ctactcagtc
     3121 agctcttagc aaggacccaa acgaaaaacg ggatcacatg gtccttcttg agttcgtcac
     3181 tgcggctggg ataacactcg ggatggacga actgtataag taggccgctc gaggagggcc
     3241 cgaacaaaaa ctcatctcag aagaggatct gaatagcgcc gtcgaccatc atcataatca
     3301 tcattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     3361 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     3421 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     3481 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     3541 cgcggccgca atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     3601 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3661 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3721 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3781 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3841 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3901 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgatgaa
     3961 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     4021 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     4081 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     4141 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     4201 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     4261 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     4321 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     4381 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     4441 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     4501 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     4561 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     4621 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     4681 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     4741 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     4801 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     4861 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     4921 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
     4981 ggtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     5041 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgcgttaac
     5101 taaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5161 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5221 cttatcatgt ctggaattga ctcaaatgat gtcaattagt ctatcagaag ctcatctggt
     5281 ctcccttccg ggggacaaga catccctgtt taatatttaa acagcagtgt tcccaaactg
     5341 ggttcttata tcccttgctc tggtcaacca ggttgcaggg tttcctgtcc tcacaggaac
     5401 gaagtcccta aagaaacagt ggcagccagg tttagccccg gaattgactg gattcctttt
     5461 ttagggccca ttggtatggc tttttccccg tatcccccca ggtgtctgca ggctcaaaga
     5521 gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc
     5581 cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg agccccgggc
     5641 ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg gctttggggg
     5701 ggggctgtcc ctgatatcta taacaagaaa atatatatat aataagttat cacgtaagta
     5761 gaacatgaaa taacaatata attatcgtat gagttaaatc ttaaaagtca cgtaaaagat
     5821 aatcatgcgt cattttgact cacgcggtcg ttatagttca aaatcagtga cacttaccgc
     5881 attgacaagc acgcctcacg ggagctccaa gcggcgactg agatgtccta aatgcacagc
     5941 gacggattcg cgctatttag aaagagagag caatatttca agaatgcatg cgtcaatttt
     6001 acgcagacta tctttctagg gttaatctag ctgcatcagg atcatatcgt cgggtctttt
     6061 ttccggctca gtcatcgccc aagctggcgc tatctgggca tcggggagga agaagcccgt
     6121 gccttttccc gcgaggttga agcggcatgg aaagagtttg ccgaggatga ctgctgctgc
     6181 attgacgttg agcgaaaacg cacgtttacc atgatgattc gggaaggtgt ggccatgcac
     6241 gcctttaacg gtgaactgtt cgttcaggcc acctgggata ccagttcgtc gcggcttttc
     6301 cggacacagt tccggatggt cagcccgaag cgcatcagca acccgaacaa taccggcgac
     6361 agccggaact gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct gggatattac
     6421 gtcagcgagg acgggtatcc tggctggatg ccgcagaaat ggacatggat accccgtgag
     6481 ttacccggcg ggcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6541 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6601 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6661 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6721 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6781 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6841 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6901 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6961 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7021 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7081 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7141 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7201 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7261 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7321 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7381 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7441 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7501 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7561 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7621 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7681 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7741 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7801 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7861 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7921 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7981 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8041 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8101 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8161 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8221 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8281 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8341 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8401 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8461 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8521 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8581 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8641 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     8701 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     8761 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     8821 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     8881 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     8941 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     9001 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     9061 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     9121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     9181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     9241 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcctcgt tcattcacgt
     9301 ttttgaaccc gtggaggacg ggcagactcg cggtgcaaat gtgttttaca gcgtgatgga
     9361 gcagatgaag atgctcgaca cgctgcagaa cacgcagcta gattaaccct agaaagataa
     9421 tcatattgtg acgtacgtta aagataatca tgtgtaaaat tgacgcatgt gttttatcgg
     9481 tctgtatatc gaggtttatt tattaatttg aatagatatt aagttttatt atatttacac
     9541 ttacatacta ataataaatt caacaaacaa tttatttatg tttatttatt tattaaaaaa
     9601 aacaaaaact caaaatttct tctataaagt aacaaaactt ttat
//
