LOCUS       sequence-467480-        9241 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac donor plasmid to overexpress ADAR2dd-PCP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 9241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: LSM0258 (PB)EF1a-ADAR2dd-PCP-T2A-PuroR-P2A-BFP
FEATURES             Location/Qualifiers
     source          1..9241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     584..603
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(822..926)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(952..1407)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1089..1110)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1301..1320
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1534..1556
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1548..1565
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1549..1565
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1695..1757
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2340..3518
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2571..3509
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3466..3486
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5543..5596
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5603..6199
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5603..5622)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6099..6119
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6200..6256
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6263..6973
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(7007..7024)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7013..7220
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7516..7550
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8041..8057)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8041..8057)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8054..8076)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8065..8081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8089..8095,8096..8113,8114..8119))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8134..8155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8272..8289)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8443..9031)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8523..8542)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9202..9241,1..752,753..821))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
       61 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
      121 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
      181 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
      241 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
      301 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
      361 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
      421 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
      481 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
      541 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
      601 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
      661 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
      721 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
      781 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
      841 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
      901 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
      961 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     1021 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     1081 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     1141 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     1201 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     1261 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     1321 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     1381 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     1441 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     1501 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     1561 ccagtgagcg cgcctcgttc attcacgttt ttgaacccgt ggaggacggg cagactcgcg
     1621 gtgcaaatgt gttttacagc gtgatggagc agatgaagat gctcgacacg ctgcagaaca
     1681 cgcagctaga ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg
     1741 tgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa
     1801 tagatattaa gttttattat atttacactt acatactaat aataaattca acaaacaatt
     1861 tatttatgtt tatttattta ttaaaaaaaa caaaaactca aaatttcttc tataaagtaa
     1921 caaaactttt atgagggaca gccccccccc aaagccccca gggatgtaat tacgtccctc
     1981 ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc gctccccccg
     2041 catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg cacgggatcg
     2101 ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg ggatacgggg
     2161 aaaaggcctc cacggccact agtttcgcac gattgcacct tgggagcctt aaccgctttt
     2221 gaatgtccgt cttagttaat ccctaattga acgctccaaa aggtgacttc caatagggat
     2281 ttattccttt taaaattaac ggcataatcg tgccgccgaa tgcaacctcg atcgagccgg
     2341 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     2401 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     2461 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     2521 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     2581 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     2641 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     2701 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     2761 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     2821 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     2881 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     2941 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     3001 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     3061 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     3121 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     3181 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     3241 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     3301 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     3361 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     3421 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     3481 tggttcaaag tttttttctt ccatttcagg tgtcgtgaat ctagaagcca ccatgttgca
     3541 cttggatcaa acgccatctc gccagcctat tcccagtgag ggtcttcagc tgcatttacc
     3601 gcaggtttta gctgacgctg tctcacgcct ggtcctgggt aagtttggtg acctgaccga
     3661 caacttctcc tcccctcacg ctcgcagaaa agtgctggct ggagtcgtca tgacaacagg
     3721 cacagatgtt aaagatgcca aggtgataag tgtttctaca ggaacaaaat gtattaatgg
     3781 tgaatacatg agtgatcgtg gccttgcatt aaatgactgc catgcagaaa taatatctcg
     3841 gagatccttg ctcagatttc tttatacaca acttgagctt tacttaaata acaaagatga
     3901 tcaaaaaaga tccatctttc agaaatcaga gcgagggggg tttaggctga aggagaatgt
     3961 ccagtttcat ctgtacatca gcacctctcc ctgtggagat gccagaatct tctcaccaca
     4021 tgagccaatc ctggaagaac cagcagatag acacccaaat cgtaaagcaa gaggacagct
     4081 acggaccaaa atagagtctg gtcaggggac gattccagtg cgctccaatg cgagcatcca
     4141 agcgtgggac ggggtgctgc aaggggagcg gctgctcacc atgtcctgca gtgacaagat
     4201 tgcacgctgg aacgtggtgg gcatccaggg atccctgctc agcattttcg tggagcccat
     4261 ttacttctcg agcatcatcc tgggcagcct ttaccacggg gaccaccttt ccagggccat
     4321 gtaccagcgg atctccaaca tagaggacct gccacctctc tacaccctca acaagccttt
     4381 gctcagtggc atcagcaatg cagaagcacg gcagccaggg aaggccccca acttcagtgt
     4441 caactggacg gtaggcgact ccgctattga ggtcatcaac gccacgactg ggaaggatga
     4501 gctgggccgc gcgtcccgcc tgtgtaagca cgcgttgtac tgtcgctgga tgcgtgtgca
     4561 cggcaaggtt ccctcccact tactacgctc caagattacc aaacccaacg tgtaccatga
     4621 gtccaagctg gcggcaaagg agtaccaggc cgccaaggcg cgtctgttca cagccttcat
     4681 caaggcgggg ctgggggcct gggtggagaa gcccaccgag caggaccagt tctcactcac
     4741 gcccggtacc ggttctgcaa ccggtggatc cggcctagct tctaaaacaa tagtgttgtc
     4801 tgtaggagaa gccaccagaa ccctcacgga gatccaaagc acagcggata gacaaatttt
     4861 cgaagagaaa gtgggaccgc ttgtcggaag actcaggttg acagcttccc tgcggcagaa
     4921 tggcgctaag accgcttata gggtgaatct caaacttgat caagcagatg ttgttgatag
     4981 cggcttgccc aaagtacgat atactcaagt ttggtcacat gatgtgacaa tcgttgcaaa
     5041 ctcaacggaa gcgagtagga aaagccttta tgatctcact aagtcactcg tcgccacctc
     5101 tcaagttgag gaccttgtcg tcaatttggt gcctcttggt cggggaggcg gttccggcgg
     5161 gggtaccctt gccagtaaga ccatcgtgtt gagcgtggga gaggcaaccc gcacactgac
     5221 agaaatccag tcaacagcag acagacagat ttttgaggag aaagtgggtc cgcttgtcgg
     5281 gcggctgcgg ctgacggctt cattgcgaca gaacggagcc aaaactgcct atcgagtcaa
     5341 ccttaaactc gatcaagcgg atgttgtgga ttccggtctg ccaaaagtac ggtacacgca
     5401 ggtgtggtcc catgacgtga ccattgtggc gaacagtacc gaggcgtctc gcaaaagcct
     5461 ttacgatttg accaaatcac tcgttgcaac ttcacaggtg gaagacctgg ttgtcaatct
     5521 tgtacctttg ggcagggcta gcgaaggtcg ggggagtttg cttacctgtg gcgatgtcga
     5581 ggagaatcca gggcccaagc ttatgaccga gtacaagccc acggtgcgcc tcgccacccg
     5641 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     5701 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     5761 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt
     5821 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     5881 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     5941 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggag tctcgcccga
     6001 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     6061 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     6121 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     6181 gacccgcaag cccggtgccg ccacgaactt ctctctgtta aagcaagcag gtgacgtgga
     6241 agaaaacccc ggtcccggat ccatggtgtc taagggcgaa gagctgatta aggagaacat
     6301 gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga
     6361 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg
     6421 ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt
     6481 catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac
     6541 atgggagaga gtcaccacat acgaggacgg gggcgtgctg accgctaccc aggacaccag
     6601 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa
     6661 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaaa cgctgtaccc
     6721 cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca
     6781 tctgatcgca aacgccaaga ccacatatag atccaagaaa cccgctaaga acctcaagat
     6841 gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgaaac
     6901 ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg
     6961 gcacaaactt aattaagaat tcctcgagct agagctcgct gatcagcctc gactgtgcct
     7021 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     7081 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     7141 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     7201 aatagcaggc atgctgggga cgacgtcagg tggcactttt cgatatctat aacaagaaaa
     7261 tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg
     7321 agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt
     7381 tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag
     7441 cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc
     7501 aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaatctagc
     7561 tgcatcagga tcatatcgtc gggtcttttt tccggctcag tcatcgccca agctggcgct
     7621 atctgggcat cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga
     7681 aagagtttgc cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc acgtttacca
     7741 tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc gttcaggcca
     7801 cctgggatac cagttcgtcg cggcttttcc ggacacagtt ccggatggtc agcccgaagc
     7861 gcatcagcaa cccgaacaat accggcgaca gccggaactg ccgtgccggt gtgcagatta
     7921 atgacagcgg tgcggcgctg ggatattacg tcagcgagga cgggtatcct ggctggatgc
     7981 cgcagaaatg gacatggata ccccgtgagt tacccggcgg gcgcgcttgg cgtaatcatg
     8041 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8101 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8161 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8221 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8281 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8341 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8401 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8461 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8521 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8581 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8641 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8701 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8761 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8821 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8881 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8941 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9001 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9061 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9121 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9181 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9241 c
//
