LOCUS       sequence-467481-       11526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac donor plasmid AVPR2-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP
            separated by EMCV IRES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 11526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: LSM042 (PB)EF1a-AVPR-ADARdd-P2A-PuroR-IRES-ARR...
FEATURES             Location/Qualifiers
     source          1..11526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1905..1967
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2550..3728
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2781..3719
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3676..3696
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6251..6304
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6311..6910
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6311..6330)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6807..6827
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(6914..7488,7489..7491,7492..7500)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7081..7098)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7308..7327
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(9502..9519)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9508..9715
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   10011..10045
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10536..10552)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10536..10552)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10549..10571)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10560..10576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10584..10590,10591..10608,10609..10614))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10629..10650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10767..10784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10938..11526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11018..11037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
     1801 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
     1861 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
     1921 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc
     1981 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
     2041 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
     2101 caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc
     2161 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
     2221 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
     2281 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
     2341 gagcctgcag acacctgggg ggatacgggg aaaaggcctc cacggccact agtttcgcac
     2401 gattgcacct tgggagcctt aaccgctttt gaatgtccgt cttagttaat ccctaattga
     2461 acgctccaaa aggtgacttc caatagggat ttattccttt taaaattaac ggcataatcg
     2521 tgccgccgaa tgcaacctcg atcgagccgg gctccggtgc ccgtcagtgg gcagagcgca
     2581 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2641 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2701 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2761 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     2821 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     2881 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     2941 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     3001 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     3061 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     3121 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     3181 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     3241 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     3301 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     3361 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     3421 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     3481 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     3541 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     3601 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     3661 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     3721 tgtcgtgaat ctagaagcca ccatgaagac gatcatcgcc ctgagctaca tcttctgcct
     3781 ggtattcgcc gactacaagg acgatgatga cgccagcatc gatatgctca tggcctctac
     3841 cacaagcgcc gtgcccggac atccatctct tccaagtctt ccctccaaca gctcacagga
     3901 gaggcctctg gatacccggg acccacttct tgcaagagcg gagctcgcct tgctctccat
     3961 cgttttcgtt gccgtcgcgt tgagcaatgg tctggtgctg gccgccctgg cgcgacgagg
     4021 ccgccgaggc cactgggcgc ctattcatgt ctttatcggc catctctgtc ttgcagacct
     4081 ggccgtggcg ctgtttcagg tgctccctca actggcttgg aaagccaccg atagattccg
     4141 ggggccagac gcactgtgtc gggccgtgaa atacctgcag atggtgggaa tgtatgccag
     4201 ttcatatatg atacttgcta tgaccttgga cagacacagg gctatctgtc gccccatgct
     4261 ggcgtatagg cacggttccg gagcccattg gaacaggcca gttctggttg cgtgggcttt
     4321 ttccctgctt ctgagcttgc cccagttgtt tattttcgca cagagaaatg tggagggcgg
     4381 atctggcgtg accgattgct gggcttgctt cgctgagcca tggggcagaa ggacctacgt
     4441 gacgtggatt gctctcatgg tgttcgttgc tcccaccctg gggatcgccg catgccaggt
     4501 cctgatattc agagaaatac acgcctcctt ggttcctggg ccaagcgagc gccctggtgg
     4561 aagaagacgg ggtcgaagga ctggctctcc aggcgaaggc gcccacgtat cagctgcggt
     4621 agctaagacc gtacgcatga cactggtcat cgtcgtcgtt tacgttcttt gctgggcccc
     4681 gttttttttg gtgcagctct gggcggcctg ggaccccgaa gcacccctgg aaggtgctcc
     4741 attcgttctg ctcatgcttc tcgcttcact gaatagctgt accaatcctt ggatttacgc
     4801 ctctttctcc tccagtgtta gctccgagct gcggagtctt ctctgttgcg cccggggcag
     4861 gaccccccca agcctgggtc cacaggacga atcctgcact accgcctcca gttccctggc
     4921 caaagacacc tcttctatcg ataccggtgg acgcacccca cccagcctgg gtccccaaga
     4981 tgagtcctgc accaccgcca gctcctccct ggccaaggac acttcatcgg gtaccggtgc
     5041 tttgcacttg gatcaaacgc catctcgcca gcctattccc agtgagggtc ttcagctgca
     5101 tttaccgcag gttttagctg acgctgtctc acgcctggtc ctgggtaagt ttggtgacct
     5161 gaccgacaac ttctcctccc ctcacgctcg cagaaaagtg ctggctggag tcgtcatgac
     5221 aacaggcaca gatgttaaag atgccaaggt gataagtgtt tctacaggaa caaaatgtat
     5281 taatggtgaa tacatgagtg atcgtggcct tgcattaaat gactgccatg cagaaataat
     5341 atctcggaga tccttgctca gatttcttta tacacaactt gagctttact taaataacaa
     5401 agatgatcaa aaaagatcca tctttcagaa atcagagcga ggggggttta ggctgaagga
     5461 gaatgtccag tttcatctgt acatcagcac ctctccctgt ggagatgcca gaatcttctc
     5521 accacatgag ccaatcctgg aagaaccagc agatagacac ccaaatcgta aagcaagagg
     5581 acagctacgg accaaaatag agtctggtca ggggacgatt ccagtgcgct ccaatgcgag
     5641 catccaagcg tgggacgggg tgctgcaagg ggagcggctg ctcaccatgt cctgcagtga
     5701 caagattgca cgctggaacg tggtgggcat ccagggatcc ctgctcagca ttttcgtgga
     5761 gcccatttac ttctcgagca tcatcctggg cagcctttac cacggggacc acctttccag
     5821 ggccatgtac cagcggatct ccaacataga ggacctgcca cctctctaca ccctcaacaa
     5881 gcctttgctc agtggcatca gcaatgcaga agcacggcag ccagggaagg cccccaactt
     5941 cagtgtcaac tggacggtag gcgactccgc tattgaggtc atcaacgcca cgactgggaa
     6001 ggatgagctg ggccgcgcgt cccgcctgtg taagcacgcg ttgtactgtc gctggatgcg
     6061 tgtgcacggc aaggttccct cccacttact acgctccaag attaccaaac ccaacgtgta
     6121 ccatgagtcc aagctggcgg caaaggagta ccaggccgcc aaggcgcgtc tgttcacagc
     6181 cttcatcaag gcggggctgg gggcctgggt ggagaagccc accgagcagg accagttctc
     6241 actcacgccc gaaggtcggg ggagtttgct tacctgtggc gatgtcgagg agaatccagg
     6301 gcccaagctt atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     6361 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     6421 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     6481 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
     6541 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     6601 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     6661 gcccaaggag cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc accagggcaa
     6721 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     6781 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     6841 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     6901 cggtgcctga tagcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     6961 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     7021 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     7081 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     7141 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     7201 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     7261 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     7321 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     7381 tggggcctcg gtacacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     7441 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc
     7501 atgggggaga aacccgggac cagggtcttc aagaagtcga gccctaactg caagctcacc
     7561 gtgtacttgg gcaagcggga cttcgtagat cacctggaca aagtggaccc tgtagatggc
     7621 gtggtgcttg tggaccctga ctacctgaag gaccgcaaag tgtttgtgac cctcacctgc
     7681 gccttccgct atggccgtga agacctggat gtgctgggct tgtccttccg caaagacctg
     7741 ttcatcgcca cctaccaggc cttccccccg gtgcccaacc caccccggcc ccccacccgc
     7801 ctgcaggacc ggctgctgag gaagctgggc cagcatgccc accccttctt cttcaccata
     7861 ccccagaatc ttccatgctc cgtcacactg cagccaggcc cagaggatac aggaaaggcc
     7921 tgcggcgtag actttgagat tcgagccttc tgtgctaaat cactagaaga gaaaagccac
     7981 aaaaggaact ctgtgcggct ggtgatccga aaggtgcagt tcgccccgga gaaacccggc
     8041 ccccagcctt cagccgaaac cacacgccac ttcctcatgt ctgaccggtc cctgcacctc
     8101 gaggcttccc tggacaagga gctgtactac catggggagc ccctcaatgt aaatgtccac
     8161 gtcaccaaca actccaccaa gaccgtcaag aagatcaaag tctctgtgag acagtacgcc
     8221 gacatctgcc tcttcagcac cgcccagtac aagtgtcctg tggctcaact cgaacaagat
     8281 gaccaggtat ctcccagctc cacattctgt aaggtgtaca ccataacccc actgctcagc
     8341 gacaaccggg agaagcgggg tctcgccctg gatgggaaac tcaagcacga ggacaccaac
     8401 ctggcttcca gcaccatcgt gaaggagggt gccaacaagg aggtgctggg aatcctggtg
     8461 tcctacaggg tcaaggtgaa gctggtggtg tctcgaggcg gggatgtctc tgtggagctg
     8521 ccttttgttc ttatgcaccc caagccccac gaccacatcc ccctccccag accccagtca
     8581 gccgctccgg agacagatgt ccctgtggac accaacctca ttgaatttga taccaactat
     8641 gccacagatg atgacattgt gtttgaggac tttgcccggc ttcggctgaa ggggatgaag
     8701 gatgacgact atgatgatca actctgcggc ggtggtagcg ctatggcgag caactttacg
     8761 caatttgtgt tggttgacaa tggtggcacc ggagacgtaa ctgttgcgcc gagcaatttt
     8821 gcgaacggga ttgcagaatg gataagtagt aacagccgga gtcaggcata taaagtcaca
     8881 tgctcagttc gccaaagttc cgcacagaac cgaaaatata ccataaaggt agaagtgcca
     8941 aaaggggcgt ggcgatctta tcttaatatg gagcttacga tcccaatctt tgcgactaat
     9001 tcagactgcg agctgattgt caaggccatg caaggtcttt tgaaagacgg taacccgatt
     9061 ccctctgcga tcgccgccaa ctcaggaata tatggtggcg gaaccggtgg cgggtccatg
     9121 gcctccaatt ttacacagtt tgtgctcgtg gacaacggcg gcactggaga cgtcacagta
     9181 gctccaagta actttgcgaa tgggatagcc gagtggatct cttccaattc aagatcacaa
     9241 gcctataagg tgacttgtag tgtacgccaa tctagtgccc agaaccggaa gtataccatt
     9301 aaagtggagg tccccaaagg tgcatggcgc tcatacttga atatggaatt gacgattccg
     9361 atatttgcga ccaatagcga ctgcgagctt attgtaaaag ccatgcaagg acttctgaaa
     9421 gatggcaacc cgataccttc cgcaatcgct gcgaatagcg gaatttacta agaattcctc
     9481 gagctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     9541 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     9601 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     9661 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggacgacg
     9721 tcaggtggca cttttcgata tctataacaa gaaaatatat atataataag ttatcacgta
     9781 agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa
     9841 agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta
     9901 ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca
     9961 cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa
    10021 ttttacgcag actatctttc tagggttaat ctagctgcat caggatcata tcgtcgggtc
    10081 ttttttccgg ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc
    10141 ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag tttgccgagg atgactgctg
    10201 ctgcattgac gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat
    10261 gcacgccttt aacggtgaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct
    10321 tttccggaca cagttccgga tggtcagccc gaagcgcatc agcaacccga acaataccgg
    10381 cgacagccgg aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata
    10441 ttacgtcagc gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg
    10501 tgagttaccc ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    10561 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    10621 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    10681 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    10741 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10801 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10861 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10921 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10981 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    11041 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    11101 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    11161 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    11221 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    11281 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    11341 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    11401 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    11461 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    11521 tctcaa
//
