LOCUS       sequence-467482-       15803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac donor plasmid with optimized VEGF-sensing eLIDAR receptor 
            components separated by EMCV IRES, Puromycin resistance and BFP as 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15803)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 15803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: XZ377 (PB)
            EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM...
FEATURES             Location/Qualifiers
     source          1..15803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1905..1967
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2457..3635
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2688..3626
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3583..3603
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3644..3653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3728..3793
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(7373..7947,7948..7950,7951..7959)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7540..7557)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7767..7786
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8026..8052
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             11195..11224
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(11280..11297)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11286..11493
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11526..11546)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11531..11860
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11711..11846
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11773..11792
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             11909..12505
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11909..11928)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12405..12425
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             12506..12562
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             12569..13279
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(13342..13362)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    13404..13525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13441..13460)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13495..13514
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   14288..14322
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(14813..14829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14813..14829)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14826..14848)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14837..14853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14861..14867,14868..14885,14886..14891))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14906..14927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15044..15061)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15215..15803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15295..15314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
     1801 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
     1861 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
     1921 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc
     1981 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
     2041 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
     2101 caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc
     2161 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
     2221 gggctccgct ccggtccggc gctccccccg catcccagag ccggcagcgt gcggggacag
     2281 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
     2341 gagcctgcag acacctgggg ggatacgggg aaaaggcctc cacggccaca attgcatgaa
     2401 gaatctgctt agggttaggc gttttgcgct gcttcgtacg ggccagatat acgcgtggct
     2461 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2521 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2581 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2641 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2701 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2761 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
     2821 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
     2881 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     2941 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     3001 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     3061 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     3121 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     3181 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     3241 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     3301 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     3361 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     3421 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     3481 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     3541 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     3601 ttcaaagttt ttttcttcca tttcaggtgt cgtgaatcta gaagccacca tggtcagcta
     3661 ctgggacacc ggggtcctgc tgtgcgcgct gctcagctgt ctgcttctca caggatctag
     3721 ttcaggtgac tacaaggatc acgatgggga ttacaaagat catgacattg actacaagga
     3781 tgacgatgat aagggtacct caaaattaaa agatcctgaa ctgagtttaa aaggcaccca
     3841 gcacatcatg caagcaggcc agacactgca tctccaatgc aggggggaag cagcccataa
     3901 atggtctttg cctgaaatgg tgagtaagga aagcgaaagg ctgagcataa ctaaatctgc
     3961 ctgtggaaga aatggcaaac aattctgcag tactttaacc ttgaacacag ctcaagcaaa
     4021 ccacactggc ttctacagct gcaaatatct agctgtacct acttcaaaga agaaggaaac
     4081 agaatctgca atctatatat ttattagtga tacaggtaga cctttcgtag agatgtacag
     4141 tgaaatcccc gaaattatac acatgactga aggaagggag ctcgtcattc cctgccgggt
     4201 tacgtcacct aacatcactg ttactttaaa aaagtttcca cttgacactt tgatccctga
     4261 tggaaaacgc ataatctggg acagtagaaa gggcttcatc atatcaaatg caacgtacaa
     4321 agaaataggg cttctgacct gtgaagcaac agtcaatggg catttgtata agacaaacta
     4381 tctcacacat cgacaaacca atacaatcat agatgtccaa ataagcacac cacgcccagt
     4441 caaattactt agaggccata ctcttgttct caattgtact gctaccactc ccttgaacac
     4501 gagagttcaa atgacctgga gttaccctga tgaaaaaaat aagagagctt ccgtaaggcg
     4561 acgaattgac caaagcaatt cccatgccaa catattctac agtgttctta ctattgacaa
     4621 aatgcagaac aaagacaaag gactttatac ttgtcgtgta aggagtggac catcattcaa
     4681 atctgttaac acctcagtgc atatatatga taaagcattc atcactgtga aacatcgaaa
     4741 acagcaggtg cttgaaaccg tagctggcaa gcggtcttac cggctctcta tgaaagtgaa
     4801 ggcatttccc tcgccggaag ttgtatggtt aaaagatggg ttacctgcga ctgagaaatc
     4861 tgctcgctat ttgactcgtg gctactcgtt aattatcaag gacgtaactg aagaggatgc
     4921 agggaattat acaatcttgc tgagcataaa acagtcaaat gtgtttaaaa acctcactgc
     4981 cactctaatt gtcaatgtga aaccccagat ttacgaaaag gctgtgtcat cgtttccaga
     5041 cccggctctc tacccactgg gcagcagaca aatcctgact tgtaccgcat atggtatccc
     5101 tcaacctaca atcaagtggt tctggcaccc ctgtaaccat aatcattccg aagcaaggtg
     5161 tgacttttgt tccaataatg aagagtcctt tatcctggat gctgacagca acatgggaaa
     5221 cagaattgag agcatcactc agcgcatggc aataatagaa ggaaagaata agatggctag
     5281 caccttggtt gtggctgact ctagaatttc tggaatctac atttgcatag cttccaataa
     5341 agttgggact gtgggaagaa acataagctt ttatatcaca gatgtgccaa atgggtttca
     5401 tgttaacttg gaaaaaatgc cgacggaagg agaggacctg aaactgtctt gcacagttaa
     5461 caagttctta tacagagacg ttacttggat tttactgcgg acagttaata acagaacaat
     5521 gcactacagt attagcaagc aaaaaatggc catcactaag gagcactcca tcactcttaa
     5581 tcttaccatc atgaatgttt ccctgcaaga ttcaggcacc tatgcctgca gagccaggaa
     5641 tgtatacaca ggggaagaaa tcctccagaa gaaagaaatt acaatcagag atcaggaagc
     5701 accatacctc ctgcgaaacc tcagtgatca cacagtggcc atcagcagtt ccaccacttt
     5761 agactgtcat gctaatggtg tccccgagcc tcagatcact tggtttaaaa acaaccacaa
     5821 aatacaacaa gagcctggaa ttattttagg accaggaagc agcacgctgt ttattgaaag
     5881 agtcacagaa gaggatgaag gtgtctatca ctgcaaagcc accaaccaga agggctctgt
     5941 ggaaagttca gcatacctca ctgttcaagg aacctcggac aagagtaatc tggagctgat
     6001 cactctaaca tgcacctgtg tggctgcgac tctcttctgg ctcctattaa ccctctttat
     6061 cctgagggcg gccgccaaga gcaggatcac cagcgagggc gagtacatcc ccctggacca
     6121 gatcgacatc aacgtgttgc acttggatca aacgccatct cgccagccta ttcccagtga
     6181 gggtcttcag ctgcatttac cgcaggtttt agctgacgct gtctcacgcc tggtcctggg
     6241 taagtttggt gacctgaccg acaacttctc ctcccctcac gctcgcagaa aagtgctggc
     6301 tggagtcgtc atgacaacag gcacagatgt taaagatgcc aaggtgataa gtgtttctac
     6361 aggaacaaaa tgtattaatg gtgaatacat gagtgatcgt ggccttgcat taaatgactg
     6421 ccatgcagaa ataatatctc ggagatcctt gctcagattt ctttatacac aacttgagct
     6481 ttacttaaat aacaaagatg atcaaaaaag atccatcttt cagaaatcag agcgaggggg
     6541 gtttaggctg aaggagaatg tccagtttca tctgtacatc agcacctctc cctgtggaga
     6601 tgccagaatc ttctcaccac atgagccaat cctggaagaa ccagcagata gacacccaaa
     6661 tcgtaaagca agaggacagc tacggaccaa aatagagtct ggtcagggga cgattccagt
     6721 gcgctccaat gcgagcatcc aagcgtggga cggggtgctg caaggggagc ggctgctcac
     6781 catgtcctgc agtgacaaga ttgcacgctg gaacgtggtg ggcatccagg gatccctgct
     6841 cagcattttc gtggagccca tttacttctc gagcatcatc ctgggcagcc tttaccacgg
     6901 ggaccacctt tccagggcca tgtaccagcg gatctccaac atagaggacc tgccacctct
     6961 ctacaccctc aacaagcctt tgctcagtgg catcagcaat gcagaagcac ggcagccagg
     7021 gaaggccccc aacttcagtg tcaactggac ggtaggcgac tccgctattg aggtcatcaa
     7081 cgccacgact gggaaggatg agctgggccg cgcgtcccgc ctgtgtaagc acgcgttgta
     7141 ctgtcgctgg atgcgtgtgc acggcaaggt tccctcccac ttactacgct ccaagattac
     7201 caaacccaac gtgtaccatg agtccaagct ggcggcaaag gagtaccagg ccgccaaggc
     7261 gcgtctgttc acagccttca tcaaggcggg gctgggggcc tgggtggaga agcccaccga
     7321 gcaggaccag ttctcactca cgcccttctg ctacgagaac gaggtgtgat agcccctctc
     7381 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     7441 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     7501 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     7561 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     7621 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     7681 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     7741 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     7801 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tacacatgct
     7861 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     7921 ttttcctttg aaaaacacga tgataatatg gccacaacca tggagagcaa ggtgctgctg
     7981 gccgtcgccc tgtggctctg cgtggagacc cgggccgcct ctgtgtaccc atacgatgtt
     8041 ccagattacg ctggtttgcc tagtgtttct cttgatctgc ccaggctcag catacaaaaa
     8101 gacatactta caattaaggc taatacaact cttcaaatta cttgcagggg acagagggac
     8161 ttggactggc tttggcccaa taatcagagt ggcagtgagc aaagggtgga ggtgactgag
     8221 tgcagcgatg gcctcttctg taagacactc acaattccaa aagtgatcgg aaatgacact
     8281 ggagcctaca agtgcttcta ccgggaaact gacttggcct cggtcattta tgtctatgtt
     8341 caagattaca gatctccatt tattgcttct gttagtgacc aacatggagt cgtgtacatt
     8401 actgagaaca aaaacaaaac tgtggtgatt ccatgtctcg ggtccatttc aaatctcaac
     8461 gtgtcacttt gtgcaagata cccagaaaag agatttgttc ctgatggtaa cagaatttcc
     8521 tgggacagca agaagggctt tactattccc agctacatga tcagctatgc tggcatggtc
     8581 ttctgtgaag caaaaattaa tgatgaaagt taccagtcta ttatgtacat agttgtcgtt
     8641 gtagggtata ggatttatga tgtggttctg agtccgtctc atggaattga actatctgtt
     8701 ggagaaaagc ttgtcttaaa ttgtacagca agaactgaac taaatgtggg gattgacttc
     8761 aactgggaat acccttcttc gaagcatcag cataagaaac ttgtaaaccg agacctaaaa
     8821 acccagtctg ggagtgagat gaagaaattt ttgagcacct taactataga tggtgtaacc
     8881 cggagtgacc aaggattgta cacctgtgca gcatccagtg ggctgatgac caagaagaac
     8941 agcacatttg tcagggtcca tgaaaaacct tttgttgctt ttggaagtgg catggaatct
     9001 ctggtggaag ccacggtggg ggagcgtgtc agaatccctg cgaagtacct tggttaccca
     9061 cccccagaaa taaaatggta taaaaatgga ataccccttg agtccaatca cacaattaaa
     9121 gcggggcatg tactgacgat tatggaagtg agtgaaagag acacaggaaa ttacactgtc
     9181 atccttacca atcccatttc aaaggagaag cagagccatg tggtctctct ggttgtgtat
     9241 gtcccacccc agattggtga gaaatctcta atctctcctg tggattccta ccagtacggc
     9301 accactcaaa cgctgacatg tacggtctat gccattcctc ccccgcatca catccactgg
     9361 tattggcagt tggaggaaga gtgcgccaac gagcccagcc aagctgtctc agtgacaaac
     9421 ccataccctt gtgaagaatg gagaagtgtg gaggacttcc agggaggaaa taaaattgaa
     9481 gttaataaaa atcaatttgc tctaattgaa ggaaaaaaca aaactgtaag tacccttgtt
     9541 atccaagcgg caaatgtgtc agctttgtac aaatgtgaag cggtcaacaa agtcgggaga
     9601 ggagagaggg tgatctcctt ccacgtgacc aggggtcctg aaattacttt gcaacctgac
     9661 atgcagccca ctgagcagga gagcgtgtct ttgtggtgca ctgcagacag atctacgttt
     9721 gagaacctca catggtacaa gcttggccca cagcctctgc caatccatgt gggagagttg
     9781 cccacacctg tttgcaagaa cttggatact ctttggaaat tgaatgccac catgttctct
     9841 aatagcacaa atgacatttt gatcatggag cttaagaatg catccttgca ggaccaagga
     9901 gactatgtct gccttgctca agacaggaag accaagaaaa gacattgcgt ggtcaggcag
     9961 ctcacagtcc tagagcgtgt ggcacccacg atcacaggaa acctggagaa tcagacgaca
    10021 agtattgggg aaagcatcga agtctcatgc acggcatctg ggaatccccc tccacagatc
    10081 atgtggttta aagataatga gacccttgta gaagactcag gcattgtatt gaaggatggg
    10141 aaccggaacc tcactatccg cagagtgagg aaggaggacg aaggcctcta cacctgccag
    10201 gcatgcagtg ttcttggctg tgcaaaagtg gaggcatttt tcataataga aggtgcccag
    10261 gaaaagacga acttggaaat cattattcta gtaggcacgg cggtgattgc catgttcttc
    10321 tggctacttc ttgtcatcat cggtaccgcg gccgccaaga gcaggatcac cagcgagggc
    10381 gagtacatcc ccctggacca gatcgacatc aacgtgatgg cgagcaactt tacgcaattt
    10441 gtgttggttg acaatggtgg caccggagac gtaactgttg cgccaagcaa ttttgcgaac
    10501 gggattgcag aatggataag tagtaacagc cggagtcagg catataaagt cacatgctca
    10561 gttcgccaaa gttccgcaca gaaccgaaaa tataccataa aggtagaagt gccaaaaggg
    10621 gcgtggcgat cttatcttaa tatggagctt acgatcccaa tctttgcgac taattcagac
    10681 tgcgagctga ttgtcaaggc catgcaaggt cttttgaaag acggtaaccc gattccctct
    10741 gcgatcgccg ccaactcagg aatatatggt ggcggaaccg gtggcgggtc catggcctcc
    10801 aattttacac agtttgtgct cgtggacaac ggcggcactg gagacgtcac agtagctcca
    10861 agtaactttg cgaatgggat agccgagtgg atctcttcca attcaagatc acaagcctat
    10921 aaggtgactt gtagtgtacg ccaatctagt gcccagaacc ggaagtatac cattaaagtg
    10981 gaggtcccca aaggtgcatg gcgctcatac ttgaatatgg aattgacgat tccgatattt
    11041 gcgaccaata gcgactgcga gcttattgta aaagccatgc aaggacttct gaaagatggc
    11101 aacccgatac cttccgcaat cgctgcgaat agcggaattt acttctgcta cgagaacgag
    11161 gtgtgatgat agccggctag ctcgaggagg gcccgaacaa aaactcatct cagaagagga
    11221 tctgaatagc gccgtcgacc atcatcataa tcatcattga gtttaaaccc gctgatcagc
    11281 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
    11341 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
    11401 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
    11461 ggattgggaa gacaatagca ggcatgctgg ggacgcggcc gcaatttaac gcgaattaat
    11521 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
    11581 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
    11641 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
    11701 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
    11761 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
    11821 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
    11881 atccattttc ggatctgatc agcacgtgat gaccgagtac aagcccacgg tgcgcctcgc
    11941 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
    12001 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
    12061 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
    12121 cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
    12181 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
    12241 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggagtctc
    12301 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
    12361 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
    12421 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
    12481 gtgcatgacc cgcaagcccg gtgccgccac gaacttctct ctgttaaagc aagcaggtga
    12541 cgtggaagaa aaccccggtc ccggatccat ggtgtctaag ggcgaagagc tgattaagga
    12601 gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac
    12661 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
    12721 gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa
    12781 gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg
    12841 cttcacatgg gagagagtca ccacatacga ggacgggggc gtgctgaccg ctacccagga
    12901 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac
    12961 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgaaacgct
    13021 gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg
    13081 gagccatctg atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct
    13141 caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa
    13201 cgaaacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa
    13261 actggggcac aaacttaatt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
    13321 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
    13381 gcctttgtat catgcgttaa ctaaacttgt ttattgcagc ttataatggt tacaaataaa
    13441 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    13501 tgtccaaact catcaatgta tcttatcatg tctggaattg actcaaatga tgtcaattag
    13561 tctatcagaa gctcatctgg tctcccttcc gggggacaag acatccctgt ttaatattta
    13621 aacagcagtg ttcccaaact gggttcttat atcccttgct ctggtcaacc aggttgcagg
    13681 gtttcctgtc ctcacaggaa cgaagtccct aaagaaacag tggcagccag gtttagcccc
    13741 ggaattgact ggattccttt tttagggccc attggtatgg ctttttcccc gtatcccccc
    13801 aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac
    13861 cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg
    13921 gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta
    13981 catccctggg ggctttgggg gggggctgtc cctgatatct ataacaagaa aatatatata
    14041 taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta tgagttaaat
    14101 cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc gttatagttc
    14161 aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca agcggcgact
    14221 gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga gcaatatttc
    14281 aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaatcta gctgcatcag
    14341 gatcatatcg tcgggtcttt tttccggctc agtcatcgcc caagctggcg ctatctgggc
    14401 atcggggagg aagaagcccg tgccttttcc cgcgaggttg aagcggcatg gaaagagttt
    14461 gccgaggatg actgctgctg cattgacgtt gagcgaaaac gcacgtttac catgatgatt
    14521 cgggaaggtg tggccatgca cgcctttaac ggtgaactgt tcgttcaggc cacctgggat
    14581 accagttcgt cgcggctttt ccggacacag ttccggatgg tcagcccgaa gcgcatcagc
    14641 aacccgaaca ataccggcga cagccggaac tgccgtgccg gtgtgcagat taatgacagc
    14701 ggtgcggcgc tgggatatta cgtcagcgag gacgggtatc ctggctggat gccgcagaaa
    14761 tggacatgga taccccgtga gttacccggc gggcgcgctt ggcgtaatca tggtcatagc
    14821 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    14881 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    14941 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    15001 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    15061 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    15121 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    15181 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    15241 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    15301 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    15361 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    15421 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    15481 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    15541 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    15601 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    15661 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    15721 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    15781 cgcgcagaaa aaaaggatct caa
//
