LOCUS       sequence-467483-       12797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac donor plasmid with optimized IL-2-sensing eLIDAR receptor 
            components separated by EMCV IRES, Puromycin resistance and BFP as 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12797)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 12797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: XZ393 (PB)
            EF1a-IL2Rbsp-FLAG-IL2Rb(ECD+TM)-TS-...
FEATURES             Location/Qualifiers
     source          1..12797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1905..1967
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2457..3635
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2688..3626
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3583..3603
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3644..3653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3648..3657
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3728..3751
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(5786..6360,6361..6363,6364..6372)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5953..5970)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6180..6199
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6448..6537
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             8189..8218
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(8274..8291)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8280..8487
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8520..8540)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8525..8854
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8705..8840
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8767..8786
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8903..9499
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8903..8922)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9399..9419
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             9500..9556
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9563..10273
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(10336..10356)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    10398..10519
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10435..10454)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10489..10508
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   11282..11316
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(11807..11823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11807..11823)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11820..11842)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11831..11847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11855..11861,11862..11879,11880..11885))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11900..11921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12038..12055)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12209..12797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12289..12308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
     1801 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
     1861 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
     1921 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc
     1981 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
     2041 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
     2101 caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc
     2161 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
     2221 gggctccgct ccggtccggc gctccccccg catcccagag ccggcagcgt gcggggacag
     2281 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
     2341 gagcctgcag acacctgggg ggatacgggg aaaaggcctc cacggccaca attgcatgaa
     2401 gaatctgctt agggttaggc gttttgcgct gcttcgtacg ggccagatat acgcgtggct
     2461 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2521 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2581 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2641 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2701 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2761 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
     2821 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
     2881 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     2941 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     3001 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     3061 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     3121 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     3181 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     3241 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     3301 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     3361 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     3421 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     3481 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     3541 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     3601 ttcaaagttt ttttcttcca tttcaggtgt cgtgaatcta gaagccacca tggcggcccc
     3661 tgctctgtcc tggcgtctgc ccctcctcat cctcctcctg cccctggcta cctcttgggc
     3721 atctgcagac tacaaggatg acgatgataa gggtaccgcg gtgaatggca cttcccagtt
     3781 cacatgcttc tacaattcga gagccaacat ctcctgtgtc tggagccaag atggggctct
     3841 gcaggacact tcctgccaag tccatgcctg gccggacaga cggcggtgga accaaacctg
     3901 tgagctgctc cccgtgagtc aagcatcctg ggcctgcaac ctgatcctcg gagccccaga
     3961 ttctcagaaa ctgaccacag ttgacatcgt caccctgagg gtgctgtgcc gtgagggggt
     4021 gcgatggagg gtgatggcca tccaggactt caagcccttt gagaaccttc gcctgatggc
     4081 ccccatctcc ctccaagttg tccacgtgga gacccacaga tgcaacataa gctgggaaat
     4141 ctcccaagcc tcccactact ttgaaagaca cctggagttc gaggcccgga cgctgtcccc
     4201 aggccacacc tgggaggagg cccccctgct gactctcaag cagaagcagg aatggatctg
     4261 cctggagacg ctcaccccag acacccagta tgagtttcag gtgcgggtca agcctctgca
     4321 aggcgagttc acgacctgga gcccctggag ccagcccctg gccttcagga caaagcctgc
     4381 agcccttggg aaggacacca ttccgtggct cggccacctc ctcgtgggcc tcagcggggc
     4441 ttttggcttc atcatcttag tgtacttgct catcggtacc gcggccgcca agagcaggat
     4501 caccagcgag ggcgagtaca tccccctgga ccagatcgac atcaacgtgt tgcacttgga
     4561 tcaaacgcca tctcgccagc ctattcccag tgagggtctt cagctgcatt taccgcaggt
     4621 tttagctgac gctgtctcac gcctggtcct gggtaagttt ggtgacctga ccgacaactt
     4681 ctcctcccct cacgctcgca gaaaagtgct ggctggagtc gtcatgacaa caggcacaga
     4741 tgttaaagat gccaaggtga taagtgtttc tacaggaaca aaatgtatta atggtgaata
     4801 catgagtgat cgtggccttg cattaaatga ctgccatgca gaaataatat ctcggagatc
     4861 cttgctcaga tttctttata cacaacttga gctttactta aataacaaag atgatcaaaa
     4921 aagatccatc tttcagaaat cagagcgagg ggggtttagg ctgaaggaga atgtccagtt
     4981 tcatctgtac atcagcacct ctccctgtgg agatgccaga atcttctcac cacatgagcc
     5041 aatcctggaa gaaccagcag atagacaccc aaatcgtaaa gcaagaggac agctacggac
     5101 caaaatagag tctggtcagg ggacgattcc agtgcgctcc aatgcgagca tccaagcgtg
     5161 ggacggggtg ctgcaagggg agcggctgct caccatgtcc tgcagtgaca agattgcacg
     5221 ctggaacgtg gtgggcatcc agggatccct gctcagcatt ttcgtggagc ccatttactt
     5281 ctcgagcatc atcctgggca gcctttacca cggggaccac ctttccaggg ccatgtacca
     5341 gcggatctcc aacatagagg acctgccacc tctctacacc ctcaacaagc ctttgctcag
     5401 tggcatcagc aatgcagaag cacggcagcc agggaaggcc cccaacttca gtgtcaactg
     5461 gacggtaggc gactccgcta ttgaggtcat caacgccacg actgggaagg atgagctggg
     5521 ccgcgcgtcc cgcctgtgta agcacgcgtt gtactgtcgc tggatgcgtg tgcacggcaa
     5581 ggttccctcc cacttactac gctccaagat taccaaaccc aacgtgtacc atgagtccaa
     5641 gctggcggca aaggagtacc aggccgccaa ggcgcgtctg ttcacagcct tcatcaaggc
     5701 ggggctgggg gcctgggtgg agaagcccac cgagcaggac cagttctcac tcacgccctt
     5761 ctgctacgag aacgaggtgt gatagcccct ctccctcccc cccccctaac gttactggcc
     5821 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     5881 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     5941 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     6001 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     6061 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     6121 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     6181 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     6241 tgggatctga tctggggcct cggtacacat gctttacatg tgtttagtcg aggttaaaaa
     6301 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     6361 atggccacaa ccatgttgaa gccatcatta ccattcacat ccctcttatt cctgcagctg
     6421 cccctgctgg gagtgggggc ggccgtttac ccatacgatg ttcctgacta tgcgggctat
     6481 ccctatgacg tcccggacta tgcaggatcc tatccatatg acgttccaga ttacgctccg
     6541 gccgccggta ccctgaacac gacaattctg acgcccaatg ggaatgaaga caccacagct
     6601 gatttcttcc tgaccactat gcccactgac tccctcagtg tttccactct gcccctccca
     6661 gaggttcagt gttttgtgtt caatgtcgag tacatgaatt gcacttggaa cagcagctct
     6721 gagccccagc ctaccaacct cactctgcat tattggtaca agaactcgga taatgataaa
     6781 gtccagaagt gcagccacta tctattctct gaagaaatca cttctggctg tcagttgcaa
     6841 aaaaaggaga tccacctcta ccaaacattt gttgttcagc tccaggaccc acgggaaccc
     6901 aggagacagg ccacacagat gctaaaactg cagaatctgg tgatcccctg ggctccagag
     6961 aacctaacac ttcacaaact gagtgaatcc cagctagaac tgaactggaa caacagattc
     7021 ttgaaccact gtttggagca cttggtgcag taccggactg actgggacca cagctggact
     7081 gaacaatcag tggattatag acataagttc tccttgccta gtgtggatgg gcagaaacgc
     7141 tacacgtttc gtgttcggag ccgctttaac ccactctgtg gaagtgctca gcattggagt
     7201 gaatggagcc acccaatcca ctgggggagc aatacttcaa aagagaatcc tttcctgttt
     7261 gcattggaag ccgtggttat ctctgttggc tccatgggat tgattatcag ccttctctgt
     7321 gtgtatttct ggctgggtac cgcggccgcc aagagcagga tcaccagcga gggcgagtac
     7381 atccccctgg accagatcga catcaacgtg atggcgagca actttacgca atttgtgttg
     7441 gttgacaatg gtggcaccgg agacgtaact gttgcgccaa gcaattttgc gaacgggatt
     7501 gcagaatgga taagtagtaa cagccggagt caggcatata aagtcacatg ctcagttcgc
     7561 caaagttccg cacagaaccg aaaatatacc ataaaggtag aagtgccaaa aggggcgtgg
     7621 cgatcttatc ttaatatgga gcttacgatc ccaatctttg cgactaattc agactgcgag
     7681 ctgattgtca aggccatgca aggtcttttg aaagacggta acccgattcc ctctgcgatc
     7741 gccgccaact caggaatata tggtggcgga accggtggcg ggtccatggc ctccaatttt
     7801 acacagtttg tgctcgtgga caacggcggc actggagacg tcacagtagc tccaagtaac
     7861 tttgcgaatg ggatagccga gtggatctct tccaattcaa gatcacaagc ctataaggtg
     7921 acttgtagtg tacgccaatc tagtgcccag aaccggaagt ataccattaa agtggaggtc
     7981 cccaaaggtg catggcgctc atacttgaat atggaattga cgattccgat atttgcgacc
     8041 aatagcgact gcgagcttat tgtaaaagcc atgcaaggac ttctgaaaga tggcaacccg
     8101 ataccttccg caatcgctgc gaatagcgga atttacttct gctacgagaa cgaggtgtga
     8161 tgatagccgg ctagctcgag gagggcccga acaaaaactc atctcagaag aggatctgaa
     8221 tagcgccgtc gaccatcatc ataatcatca ttgagtttaa acccgctgat cagcctcgac
     8281 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     8341 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     8401 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     8461 ggaagacaat agcaggcatg ctggggacgc ggccgcaatt taacgcgaat taattctgtg
     8521 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8581 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8641 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8701 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8761 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     8821 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     8881 tttcggatct gatcagcacg tgatgaccga gtacaagccc acggtgcgcc tcgccacccg
     8941 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     9001 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     9061 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt
     9121 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     9181 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     9241 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggag tctcgcccga
     9301 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     9361 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     9421 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     9481 gacccgcaag cccggtgccg ccacgaactt ctctctgtta aagcaagcag gtgacgtgga
     9541 agaaaacccc ggtcccggat ccatggtgtc taagggcgaa gagctgatta aggagaacat
     9601 gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga
     9661 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg
     9721 ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt
     9781 catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac
     9841 atgggagaga gtcaccacat acgaggacgg gggcgtgctg accgctaccc aggacaccag
     9901 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa
     9961 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaaa cgctgtaccc
    10021 cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca
    10081 tctgatcgca aacgccaaga ccacatatag atccaagaaa cccgctaaga acctcaagat
    10141 gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgaaac
    10201 ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg
    10261 gcacaaactt aattaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
    10321 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
    10381 gtatcatgcg ttaactaaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    10441 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    10501 aactcatcaa tgtatcttat catgtctgga attgactcaa atgatgtcaa ttagtctatc
    10561 agaagctcat ctggtctccc ttccggggga caagacatcc ctgtttaata tttaaacagc
    10621 agtgttccca aactgggttc ttatatccct tgctctggtc aaccaggttg cagggtttcc
    10681 tgtcctcaca ggaacgaagt ccctaaagaa acagtggcag ccaggtttag ccccggaatt
    10741 gactggattc cttttttagg gcccattggt atggcttttt ccccgtatcc ccccaggtgt
    10801 ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc
    10861 cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc gccggaccgg
    10921 agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta attacatccc
    10981 tgggggcttt gggggggggc tgtccctgat atctataaca agaaaatata tatataataa
    11041 gttatcacgt aagtagaaca tgaaataaca atataattat cgtatgagtt aaatcttaaa
    11101 agtcacgtaa aagataatca tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc
    11161 agtgacactt accgcattga caagcacgcc tcacgggagc tccaagcggc gactgagatg
    11221 tcctaaatgc acagcgacgg attcgcgcta tttagaaaga gagagcaata tttcaagaat
    11281 gcatgcgtca attttacgca gactatcttt ctagggttaa tctagctgca tcaggatcat
    11341 atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg
    11401 gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag
    11461 gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa
    11521 ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt
    11581 tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg
    11641 aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg
    11701 gcgctgggat attacgtcag cgaggacggg tatcctggct ggatgccgca gaaatggaca
    11761 tggatacccc gtgagttacc cggcgggcgc gcttggcgta atcatggtca tagctgtttc
    11821 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    11881 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    11941 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    12001 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    12061 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    12121 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    12181 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    12241 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    12301 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    12361 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    12421 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    12481 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    12541 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    12601 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    12661 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    12721 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    12781 gaaaaaaagg atctcaa
//
