LOCUS       sequence-467485-       20685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate 
            inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for 
            protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20685)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 20685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF10-Pct3-(+)-Eddh(cHis6)-Accs(ElA2)
FEATURES             Location/Qualifiers
     source          1..20685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(1497..2108)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2512..2539
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             5701..5718
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      14309..14336
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      14468..14495
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(14927..15778)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(15765..16604)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(17115..18086)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            19325..19412
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      19754..20148
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(20311..20354)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            20450..20559
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctag
     1501 cgcttgaatt tcgcgtaccg ctctcgcgca atttcgagtg tcgagttcct gacacgctca
     1561 aagcgttctt tgtccttctg ccataggcta cgaacagcaa gcccacgcac cgaattgaat
     1621 atcaaccaaa ggatatcttc tgcatcatca cgcgaaagac cacggctcac cagaacacca
     1681 agccacatat cctcgacgac aaaccgattc ctctctaccg tgcgctgaat accctcgcgt
     1741 aacgctgggt cccggtcggc agccacaatc aaatcaaggc tgatagagaa gtcatcgtcg
     1801 aggaaaaatt cggcggcgtc gtccagcatt tgctggatga cgtcatcctc tggcttcaat
     1861 ttcgctaatc gagcccgact gcgttcggtg atctgttcgt aaagccattc aaaagtggca
     1921 agcagaagct caagctttgt cgggaaatga tggctctgcg ctcctctgga gacaccagca
     1981 gcaccgggca catctgcgat ccggaatccc gcgtaacctt tttcccgtaa aacccccagg
     2041 gccgctgcaa tcaacttgcc ctgggtctcc attgcgcgct ctgcctgtgt tcttctcttt
     2101 ggactcatga tcaccagcga cctgctttcg ttcgtccggg catcacccgg gttactatct
     2161 agtatcaata actagattat tggttgaaac gtactatgtc aagcagttct aaagattgac
     2221 tccaaacggt cgccttgcta tgctcgtttc gtttcctccg cacgcgcgcg aaaggctcct
     2281 tcacggagcc aatgtacgca aaaaaccccg cttcagcggg gttttttcgc caaaaaaaac
     2341 cccgccctgt caggggcggg gttttttttt ttagggccct actgactgtg agcccgctcg
     2401 atcaacggat cggtaaaata cgggcgaaaa agagaatccc gagctttttt gttgccgcat
     2461 agcgtatagt aagcatgtgc cctcgaggtg agggcgagtg ttgcggccag taacaaacag
     2521 acaatctggt ctgtttgtat tatgcagcac acagtgttac ctcctcgggg acgaggagag
     2581 gaaagaggat ttaaaatggg aaagctcaat ctgacgcgcc gtgcgttcac gaagctcacg
     2641 gccgtgacgg gtgcggcgtt ggcctgcgcg gcagcggtcg ccccgaacgc ggcgctggcc
     2701 gaagatgccg gagcggccgc gcgcggcgac gacgtgaaac gcgtgcgcac gtgctgccgc
     2761 ggctgcggaa agatggagtg cggcgtgtgg gtgaccgtgc aaaacggccg cgctatcaag
     2821 gtggagggcg accagtcgtc attccagtcg agcggcaact gctgcggcaa gtcgcagtcg
     2881 tccatccagg cggcgtacca ccccgaccgc atctaccatc ccatgaagcg cacgaatccc
     2941 aagggcgagg atcccggctg gcagcgcatc acgtgggacg aggcgatgga gatcgtcggc
     3001 accaagttca acgagctgat ggaccgctac ggcggacagt gcatcttcaa catggcgggc
     3061 acgtcgcgcc agtgggtgta cgggccctac gcgttctaca agtggctgtt cgacacgccg
     3121 aacgcgcacg tggcgtcgga aatctgcaag gggccgcgcc gtttgatggg gtggatcagc
     3181 tcggtcgacg gcgcgccgtg gatggcgctg cgcgacgggc cgcgcgtgta cgtgcagtgg
     3241 gggaccgcgc ccgaaaactc gaactacgat gacagctgcc gcaacctcgt ggacaagatg
     3301 accgaggccg acgtgcacat ctgcatcgat ccgcgcctgt ccggctcggg caaggaggcc
     3361 gactactggc tgaacctgcg tcccggctcc gacggcgcgc tggcgctgtg ctggcagcac
     3421 ctcgtcatca agaacgacct ggtggactgg gagttcgtga agcgctggac gaactcgtcg
     3481 ttcctcgtgg tggaggaccg ggagcccacg ggcggccgct acatcgacct gtccacgccg
     3541 ctcaacaacg ccggcatccc cgccgacgtc gtgggcacga agctcaagac gcgcctgctg
     3601 aaggaaagcg atgtggtgga gggcggcagc ccgcgcaagt tctacgcgtg gaacaaactg
     3661 gccaacgacg gcgccggcgg cttggtgatg tgggacgtcg acaccacgca atgggagggc
     3721 tgcaaccacg tggcccccac gcgcgaccag atggaggtcg tgtacaaggg cacgtcgcag
     3781 gagggctatt tgccgccgct gtcctatcac gagctggagg aagccggcat cgacctggat
     3841 atgcgtggga cgcatgaggt ggagctgctc gacggttcga agcacacggc gaagccggtg
     3901 tgggcgtacc tcgaggaatc ggtggcggac tgcacgcccg cgtggtgctc cgagatcacg
     3961 ggcctcgatc ctgcgctcat cgaagaggcg tgcctcgtgt gggccacgcg tcccgagggg
     4021 caggattacg gcaacggcgg tatccacctg aacctcgcgc ccgaccagat cggcaactgc
     4081 acgcagacgg tgcgcgcggt gctgcacctc atctacatga ccggcaactt cgacacgccc
     4141 gccggcaacc gcggcctcac gcgctcgccc atcgacgagc aggccacggc cgcgcccggc
     4201 tcgaacatgc cgcaggaagt gaaggcccag ctgatcgcct tgggcgagat ccccgtggaa
     4261 ggcgtcacgc ccgatcccct caacgtgccc gaccgctacg acacgctgtc gaacatggtg
     4321 ggcgccgacg agttccccat cacggcgtac tacaacgagt gggccgacgc gacgcgcatc
     4381 tgggacgcct gcctcaccgg cgagccgtac cccgtgcgcg gcggcatcaa cgagtccggc
     4441 tcgttcatga acatgtcgaa cgcgaacctg gcctgggagg cgttgcagtc gctcgatttc
     4501 tgggtggaca tcaacatgtt ccaccatccc ggcaccgaga tggccgacat cctgctgccg
     4561 tgccagcatt ggctggagat caacaacatc cgcgtgtcgc agggcgcgtc cggcggtatc
     4621 ggcgccacca tccgcgcggt cgagccgccc agcgacacga agttcgacta cgacatcaac
     4681 cgcctgctgt tcgacgccgt gggcggcccg aacggaacct ggaccaacat cgcgggcgac
     4741 gcgcccggcg gctaccacgt ggacgagcgc ttggaggact ggttccagaa caactcgaag
     4801 accaatccca aggtgaaatg gcagcattgg gacgacttcg tggaggactt tcaggagaac
     4861 ggctggatca acgccaagga gatcgagccc gaccgctggg gcacgtaccg ccgcttcgag
     4921 accggctgga tgcgcatcgg caaggacgcg tgcaccggct ccacgttcag cgcggcgttc
     4981 gacgacgccg gcaatccggt gaacaacttc ggctgcccca cgccgacggg cctcgtggaa
     5041 ttttggccgc tcgtgttcga gacgtactgc gtggacaagg cgaacgagtt caaccccggc
     5101 aagttcgacc tggtgcacga gatgatgccg cactacgacg agccgaaatc gggccccaag
     5161 ggcgacgtgg acatgaacga gtatcccatt atcctcacca ccggccgccg catacccgtg
     5221 tacttccatt ccgagcaccg gcagctgccg tggtgtcgcg agctgtggcc ggcaccgcgt
     5281 ttggaggtga accccgaaga cgctgccgag ctggggctcg agcagggcga ttgggcctgg
     5341 atcgagacgg agtggggcaa ggtgcgccag tgcgtcgatt tgtactacgg catcgcgaag
     5401 gggtgggcga acgccgagca cgcctggtgg ttccccgagc tgcccgcgcc gacgcacggg
     5461 ttcgagctgt cgaacatcga gtgcatctgg aacccctacg gccaggatcc gttcatcggg
     5521 tcgtcccaca tgcgcggcgt gcccgtgaag atatacaagg ccacgcccga gaactgcccc
     5581 gacggcaagg tcatcccctg cgcgcccgag gacggcaccg agatcatcta cgatgcctcc
     5641 gacccgcgcc tgaaggaatg gctgccgaac tacaccatcc gagaggaggc gggcagcagc
     5701 catcaccatc accatcacta aagccatgac ccagtacgcg atcgtcaccg atttgaaccg
     5761 gtgcgtcggc tgcctggcct gcatggttgc ctgcaaggcg gtcaacaacg tgccggtggg
     5821 caactattgg aacaaggtgc tgcgcatcgg cccctcgctg aaagaggggg ccacgtcgtc
     5881 gcacgacgtg gagatgtact acctgcccgt gcagtgccag cactgcgccg accccgagtg
     5941 cgtgaaggtg tgcccgacgg gcgcctcgca caaactggag gacggcacgg tgcagatcga
     6001 caaggcgaag tgcatcggct gccagttctg cgcgatgtcg tgcccctaca acgtgcggta
     6061 cctgaacgag gaagagggcg tcgtggagaa gtgcacgctg tgcgcgcaga tcgtggggca
     6121 gggcgggttg ccgcaatgcg tcatccagtg cggagggcgc gcccggttct tcggcgatct
     6181 ggaccagggg atcgagtcgt tcgaagcccc cgccgtcggc tacgacaccg atcgcaccta
     6241 cgacggcctg tatgcaggca accgcgtgac gctgggcgag ttggcgcagg acttcgatgc
     6301 gagcaccgat ctgcatcatc tgccgaacgt cggcaacgat ccgaacttcg cgttcatcct
     6361 gcgcaaccgc gactggaagg gtgatgaata atggaactcc agtggccttt gattttgttc
     6421 accacgctgg ttgcgtggag cgcggggctg ttcgggacgc aggcgctgat ggcggtgttc
     6481 ggcgtcggga agaaggcgca ggtgccggcg tgggttgcgt cggccgtctt gctggcggcc
     6541 ggcgggatcg cggtgttctt ccaccttgag cactgggagc ggatcttcaa cgggttcggg
     6601 catttgacga gcgggatcac gcaggagctg atcgcgatcg tggtgcttgc cgtggccgcc
     6661 gtggcctacc tcgtgctcat gcgcaagtcc gacgacggcg cgagcgtgcc gaagtggctg
     6721 gcgtggctgt ccgtcgcctt gtcggtcgtg ctggtcgccg tcatggcgca cagctacacg
     6781 atggccgccc gccccgcctg ggactcggcc ctctggatcc tctacgtcct cggcaacgcc
     6841 tgcgtgttgg gccccgcgac gttcgccctc ctctccacct tggctgcagg gggaccccgg
     6901 gaccaacccg ccgatcgcgc agccgacgcc ggggcccccg caggtaggac ggccctcgcc
     6961 ggcgccgcca tcaacgccct cgcaaccctc gccttcgccg ccttcctcca gctctccgcc
     7021 ggctccttcg ccgacgtcgg cctctacttc gacccgacgc accccaccaa ggccatggcc
     7081 gacgcagccg cgacggtcgc ctcccaggcc ccgctgctgt ggctgggagc cgtggccgtg
     7141 ggcgcgatcg tgcccctggc cgccgccttc atgggcaggc gcacggggaa ctggaggctc
     7201 tgggccccgg tcgcgatagc cgccgccctc gtcggcgccg tctgcatgcg cgccgtcttc
     7261 tacaacctgg ggctcagcgt gttcatgttc tactagacta gtccgctggt tgccacgacg
     7321 ccctcgaagt ctccttcgag ggcgttctgt cgtttgcaag aagggtctgg agaggcaaat
     7381 cgcgctcgaa ggggcttcga ggccggggta ggtcgggagg gtggaatccg gatgtttcac
     7441 gtgaaacatc cgttctttta cggccggtgt ttcgcgtgaa acattttgcg gaaaggactc
     7501 cttcccttcc cgtgcgccac cccaacgccc cgctcctagc gctcgtacac ctggtcgcag
     7561 ttgagcagat ccttcatgga gtgccagatc tggtgcgggt tgcccggcgg gttcttgagc
     7621 ggcttcatcg gatagcgctc ctgcgcgccc gacagcagat cgacggcgaa cacttcgtcg
     7681 gacagctcga gcgcgccttc cgggcagatg tcggtgcagc agcggcactt cacgcaatcg
     7741 accggacgat gcgtcacgcc gatcgagccg tcctcacccg cgtacttcgc gatggccccg
     7801 gtggggcaga acgtggcgca catctggcac gacgagcact tctcgggatc gatgatcaca
     7861 tggccccaca agcgcgtgtc catgagcaca tcctgcggct gccccaacga atcgagcgcg
     7921 gccagcaaac gttcgcggcg gtcgggaatg aagtgaggca gcgtgccgtc cttgcccacc
     7981 ttcacgaacc tcggctcccg ccgctcctcc acacccaatg cgtccttgac ggcgtagtca
     8041 gccgtcaccg ccgcaacgtt cttagcctcg tccttcatgc tggagaagaa gctccgacgg
     8101 ctcgcgtcgt agccctcgtc gcccgcccgg cgcacggatg agggaagctt ttccgccagg
     8161 tcgatgcgca tatcgttgtt ccaggttgca agcagcgcgc ttgccgtgtc gcacacaacc
     8221 cgcgccatct cgaaacccga gacatgctcg cattgcgcgc agcgcgcctg cacgaggctc
     8281 acgcggcgca cgcccaacgc cgccagcgtg accagcaggc tctcctccac gcgccccagg
     8341 cacgtgacgc ccaccacctt ctcggggtcg tacaacccgt cggccgcggc aagcagctga
     8401 tcgcaggcga taaccacctg tccgtcggcc acgtcagccg ccgcgcggca tgcttgcaga
     8461 agctcggcat cgttcggacg atgcgcttca agcgcgcagg tgggacacac ggtggcgcag
     8521 gttccgcacc cgatgcaccg ctcgggttcg atgaccagct cgttgtcgtc ataggagatg
     8581 caaccgctcg tgcacacgtc ggcgcacttc atgcacgttg cgttgcgatt gcgcacaata
     8641 gcgcaacgac cctgatgaac cgtaatgtcg gcgctctgaa gttgttcgag cagaccgagg
     8701 aaattcgtca cgtgagccat gacttcaccc tttctcgtct acccccttgc accgcacagt
     8761 ataaccactt tcgccgtctc acgcgagaaa aaacgcgctt gacaccttcg acaggcctcc
     8821 ctatcatgtg gccttgggga gtccgccggc acgctgatcg cgtgcgaagc ggacattgtt
     8881 tgcaaaagtg ggaacctcag gggtgaggtt tcgcggtgct gtgcgccgta agtgcaaaca
     8941 atgtctgacg aggggcggaa catgactcac gattccacga accagcatct ttctcttgag
     9001 cccgactacg acgggttcga tccacttgaa gatttcgaag acgatgcgac cgaggtcgaa
     9061 ggccgtgccg acgagttcgt cgccacggtg agcgccgccg aagacgcgct cgctcacgac
     9121 gcgatggcac ccgccacgcc cgccatctgc gaaaagcccg ctgaagagcg catcgccgac
     9181 ttgttggagg ccatggcgcc gcgccgcacg gtgctgctgg gcatcctggc cgcctgcgcc
     9241 gagccgcagc cggcatccgc cgtgaacgct cgcgtcgacg agctgcagga gaacaacttc
     9301 tcggtgtaca gcgcggccaa cctgtgctcc ttgctggaga aggcgggcgc catcgagcgc
     9361 gtcacggccg agggcgaacc cgcggaggac atcgaaatcg agccacagac cgtcgtcgtc
     9421 gacggcgtcg aatacctgga agcccgcgaa cccgtcgaga tctactggca catcaccgag
     9481 ccgggccgcg cggcgcttga ggccgacaaa ccgctcgagc gcctgcgcgc cctcctggac
     9541 gaagatgccg cctacgcgcc catctaccag cgcatcctcc gcttgtgcac agcagacggc
     9601 ggcgccacga cacccgccat caacaacgcg gtcgaccaca acccgctggt gcagaagccg
     9661 cgcttctacg cgccccactt cgtcgaccgc ctggagaaat gcgacgcgct ggcctggaag
     9721 aaggcgtggt gcatcaccga catcggacgc gccggcctcg atatgctggc agacgtcgta
     9781 gacgagaacg cgcccgccac ccagcccgaa acccccgcaa cccctgatcc cgccgcatcc
     9841 aaggaggact aaccatgacc gaagcaaccg cgcccgcaac cgagaccctc gccgacgaag
     9901 cgaccatgca ggagctcgtc aaactgaacg agcagcgcgc cgccacgtac ggcctgctgt
     9961 cgcgcctgta ccgcgtggag gtggaccagc cccttctcga cgagctgcgc agcatgcgct
    10021 tccccgccaa gacgggcaac gagaacgtgg acgagggcta ccgcctgctg gccacgtacc
    10081 tgagcaacac ctgggacaac agcgtcaccg acctcgccat cgactacgtg cgcgtgttca
    10141 tcggacacgg catcgacgcg ttctcggccg cctacccgtt cgaatcggtg tacacctccg
    10201 agaagcgcct gctcatgcag gaagcacgcg acgaggtgct ggccatctac cgcagcgcag
    10261 gcctcgacaa gcaggacaca tggaaagagg gcgaggatca tatcgcgctc gagctggaat
    10321 tcgagcagat catggccacg cgcacggtcg aagcgctgcg caaaggcgac gaagacgagg
    10381 ccgtcgcgct gctgaccacg cagaagaact ttctcgaaga ccatctggcg gcatgggctc
    10441 ccatgatgac ctccgacatg aagcgattcg cgcaaaccga cttctaccgg ggcttggcgt
    10501 acttgacgga cgggttcctc caaaccgacc aaacgttcct cgacgacgtg ctggccgaag
    10561 actaactgca caggatcgaa agaaggagcg atggacaaca agcaggagaa aacctacgcc
    10621 ggcatgacgc gacgcacgct gtgcctgggc gcaggcggcg ctgcggtcat gctaggtcta
    10681 ggcggattga agtacgcggg atcgcaaccg ctcgtgcgcc cgccgggagg ccaggacgag
    10741 gaacggctca tctcggcctg catccgctgc gagaagtgct acgagatctg cccgcgcgac
    10801 gtcatcgcgc ccgcccacct cgaggacggt gtgctgaaca tgcgcacccc cacgttcgac
    10861 ttctcggcca actggtgcga ctggtgcgcc gaagagcacg acggcacccc gttgtgcgtg
    10921 agcgcctgcc ccaccgaagc gctcaagctg cccgacgacg ccacggccga gaacaccatc
    10981 atcggcaaag ccgttatcaa cacggactgg tgcttggcgt acaagctgat cggatgccgg
    11041 ttttgctacg acgcctgtcc ctacgaggcc atggagctcg acgagaacaa ccgccccgtc
    11101 gtgatcgagg acaagtgcaa cggctgcggc gcgtgcgaga gcgtgtgcgt ctcgctgcag
    11161 aacggctcca tctccgaagg agccaccgcg cgcgccatcg tcgtccaccc gcttgacgaa
    11221 gttgaaggat aaggtgccct catgaagaag aactccaaga agctgcgcac cctcgtcgca
    11281 ctggccatcg tggccgtcgt cgccgtcggc ttcgtcacga acgtcgggat cggcaccatg
    11341 tcggcaccgg gcatctggga catctccatc ctatgcccgc tgggcgcctt gggaacgatg
    11401 ctcgcctcca agatgatggt gccgcgcgcc gtaatctcgc tgatcgtgat ggtgctgctc
    11461 atcgttctgt tcgcccgcgc gttctgcgga tggatttgcc ccgtgccgct cgtgcagaag
    11521 ttgcgcggct tgttcgcgaa gaagcaaccc gtcgaacgcg gcgacgatgc cgatcctgcc
    11581 gcggcgccgc tcacgaaagt cgagatcgac gcgctgggcg tttcatgctc gaaggggtcg
    11641 aagaatggca gcggatgcgc gtcctgcgcc tccaagcgcg gagccgcgct cgatgctcgc
    11701 cacttcgtgc tgggcggcgc gctggcgtcc accttcatct ttggattccc ggtgttctgc
    11761 ctcgtctgcc ccatcggact cacgttcgcc accatcctgc tggtcatcaa cctgttctca
    11821 cacggcgatg tcacctggtc gctcatcgtg gtgcccgcgc tgcttatcgc cgaggtgctg
    11881 ctgttcaaga agtggtgcca taagctgtgc ccgctttcgg cgttcatgag cctgatcgcg
    11941 aaggcgaaca agaccttcgt gcccacgatc gacgatgcga agtgcctgga aacggcgaag
    12001 ggcggcgcgt gcggggcctg cggccgcgtc tgcgaggagg gcatcgaccc gcgccacccg
    12061 cagctttcgc aggcggcatg gagcgagtgc accaagtgcc gcgcctgcgt ggacgcatgc
    12121 cctgcgaacg cgatcaccat gccgctgctg ccgcggcgaa cgccgtctac cgcagaacct
    12181 gtcgccgtgc caacgaacgc cgagaagatc gacgcggaga atgcgcgccc cgttccgtaa
    12241 agcccctcct gcgcgacccg gctcgcagct gcagcggtct ggtcgcgcat ccggaatcgg
    12301 gccgagcccg accccgggca aaaggcggct tggcaagggc tttgggggac gagccgcctt
    12361 ttgcccggga gcgacatccc cgctccccct ccctgcgaaa agagggatgc cgcttccggc
    12421 cttcatctga caaccacgac gtgggaggac gaaaacccat ggcatacgaa acgacaagcg
    12481 aatcgaccga ggccctgctc gtcaccggag gcgtcggcac cggcaaaacg caacgtctcg
    12541 tcgagcgcgc tgccgcgctg ctggccgaaa acgccgccgc gggcaacgtg ctcgtgctgt
    12601 gcgcgacgcc ccaggccgca cgcctgttct cggagcggtt gacagcccgc gtcggtgcca
    12661 agcgcgctgc cggcgcaacc gtcgccaccg cacgcgcctt agcgctcgac gtgctggccg
    12721 acgacggcgc gcgccgctgg tcggggcgcg agccgcgact actgaccgcc ttcgaagaga
    12781 cgttcctcat ggaggacatg aaggtgagcg ggctgcggcc gaagcggctg cgcgagatgc
    12841 tcaagttctt ctaccgctcg tggaccgagc tggcggacga cgatcccgat tggctgctac
    12901 cgggcgagga ggccgatgtc catacgctgt tgaaagagaa cctggtcttc atgcgcgccg
    12961 tcatagaacc cgaagcggcg aaccttgctg cacgctacct ccgagaacac gacgaagcgc
    13021 gggcggcgca ttcctacgcg cacgtgctga tggacgacta cgcctgcgca agccgtgcct
    13081 cgcagacgct ggcgaatctc gtcgcaacct gctcgatcac cgtggcgggc gacgcggaaa
    13141 cctgcatcga gacatacgat tcgtacccct atgccgccgg aatcgacgaa ttcctcgcaa
    13201 cgcatcccga tgctgcttgc gagcacctgg atgccgacgc gcccgatgcc gtcgaacggg
    13261 tcgctctggc aagcgacacg cccgaagccg agttcgaaca ggtggcgcgc acggtggccg
    13321 aagccgtcgc cgcaggaacg cccgcacggc agatcgtcgt ggccgtaccg aacagcgtgt
    13381 ggtcgcgcaa catcgcaacc gctttgcacg cacgaggcgt gccaaccgaa gcgctcgcca
    13441 tctcgcagcc cctgcgcggc gacgtgcggg acaacgcccg ctgcacgcct gcgcgcatcg
    13501 ccacggccct caacctcgtg gcgaacccag acgacaccgc ggcttggcgg tgctggtgtg
    13561 gattcggcga ctacctggtg aacagcgcgg ctttcgccag cctgcggact tgcgcgaagg
    13621 agcgcagtat cggcctcgtg gacgctttgg agatgctaag cgaaaacgac tgcgagcacg
    13681 tagtgggcgc gcaacgcgtg gcgaccgcct acaaggacgg tcgcgcgctc atcgagcagg
    13741 tacgaggatt gcggggcacg gcactgctgg acgagctgac gcgcatcgtc accgacggag
    13801 cggaatccca agcgcccgcc gttctgcgcg agctctgcct cgagcacggc aacgcagccg
    13861 atgaaaaccc ggaaagcctt tacgatgccg atgctgcggc gatggcgcgg cgcatggccg
    13921 agcgcctgct cgcgcccacg ctggaggttg ccgacgcggt agccgtcgtc ccctacgact
    13981 tcgcctgcgg cttctcgccc gacctgctga tcgtcgccgg cttcgtgaac ggtttcatcc
    14041 cctcccgcga ctatttcgac gccacggtga ccccgcccga gcgccaggag aagatgcgcg
    14101 ccgtcgacga acgacgcgtg cgcgcgctca caggaaaggc cggccgcgcg gtggcgctgt
    14161 cgtacttcac gtccaccgac ctcgaaagcg ccggcaagct gaagctcaag atcagtcgca
    14221 tccgcctcaa gaacggtatc cgcacctgca ctatagagcc cagcctattc ctggagcaaa
    14281 tcgcagcgca ggagtagcga ctcacactgg ctcaccttcg ggtgggcctt tctgcgttta
    14341 tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta ttatttaatt
    14401 cctgttataa aaaaaggatc aattttgaac tctctcccaa agttgatccc ttaacgatcc
    14461 ctgcaggaga aggccatcct gacggatggc ctttttgaag ctttctagaa tcgggggccc
    14521 ccggggggat tctagaacaa tggaagtcct cgtctcgccc tcgaattagc ccgcttaatg
    14581 agcgggcttt ttttgaatta attctcgcga gctggcacga caggtttccc gactggaaag
    14641 cgggcagtga gcgcaacgca attaatgtaa gttagcgcga attgcaagct ggccgacgcg
    14701 ctgggctacg tcttgctggc gttcgggagc agaagagcat acatctggaa gcaaagccag
    14761 gaaagcggcc tatggagctg tgcggcagcg ctcagtaggc aatttttcaa aatattgtta
    14821 agccttttct gagcatggta tttttcatgg tattaccaat tagcaggaaa ataagccatt
    14881 gaatataaaa gataaaaatg tcttgtttac aatagagtgg ggggggtcag cctgccgcct
    14941 tgggccgggt gatgtcgtac ttgcccgccg cgaactcggt taccgtccag cccagcgcga
    15001 ccagctccgg caacgcctcg cgcacccgct ggcggcgctt gcgcatggtc gaaccactgg
    15061 cctctgacgg ccagacatag ccgcacaagg tatctatgga agccttgccg gttttgccgg
    15121 ggtcgatcca gccacacagc cgctggtgca gcaggcgggc ggtttcgctg tccagcgccc
    15181 gcacctcgtc catgctgatg cgcacatgct ggccgccacc catgacggcc tgcgcgatca
    15241 aggggttcag ggccacgtac aggcgcccgt ccgcctcgtc gctggcgtac tccgacagca
    15301 gccgaaaccc ctgccgcttg cggccattct gggcgatgat ggataccttc caaaggcgct
    15361 cgatgcagtc ctgtatgtgc ttgagcgccc caccactatc gacctctgcc ccgatttcct
    15421 ttgccagcgc ccgatagcta cctttgacca ccatggcatc agcggtgacg gcctcccact
    15481 tgggttccag gaacagccgg agctgccgtc cgccttcggt cttgggttcc gggccaagca
    15541 ctaggccatt aggcccagcc atggccacca gcccttgcag gatgcgcaga tcatcagcgc
    15601 ccagcggctc cgggccgctg aactcgatcc gcttgccgtc gccgtagtca tacgtcacgt
    15661 ccagcttgct gcgcttgcgc tcgccccgct tgagggcacg gaacaggccg ggggccagac
    15721 agtgcgccgg gtcgtgtcgg acgtggctga ggctgtgctt gttcttaggc ttcaccacgg
    15781 ggcaccccct tgctcttgcg ctgcctctcc agcacggcgg gcttgagcac cccgccgtca
    15841 tgccgcctga accaccgatc agcgaacggt gcgccatagt tggccttgct cacaccgaag
    15901 cggacgaaga accggcgctg gtcgtcgtcc acaccccatt cctcggcctc ggcgctggtc
    15961 atgctcgaca ggtaggactg ccagcggatg ttatcgacca gtaccgagct gccccggctg
    16021 gcctgctgct ggtcgcctgc gcccatcatg gccgcgccct tgctggcatg gtgcaggaac
    16081 acgatagagc acccggtatc ggcggcgatg gcctccatgc gaccgatgac ctgggccatg
    16141 gggccgctgg cgttttcttc ctcgatgtgg aaccggcgca gcgtgtccag caccatcagg
    16201 cggcggccct cggcggcgcg cttgaggccg tcgaaccact ccggggccat gatgttgggc
    16261 aggctgccga tcagcggctg gatcagcagg ccgtcagcca cggcttgccg ttcctcggcg
    16321 ctgaggtgcg ccccaagggc gtgcaggcgg tgatgaatgg cggtgggcgg gtcttcggcg
    16381 ggcaggtaga tcaccgggcc ggtgggcagt tcgcccacct ccagcagatc cggcccgcct
    16441 gcaatctgtg cggccagttg cagggccagc atggatttac cggcaccacc gggcgacacc
    16501 agcgccccga ccgtaccggc caccatgttg ggcaaaacgt agtccagcgg tggcggcgct
    16561 gctgcgaacg cctccagaat attgataggc ttatgggtag ccattgattg cctcctttgc
    16621 aggcagttgg tggttaggcg ctggcggggt cactaccccc gccctgcgcc gctctgagtt
    16681 cttccaggca ctcgcgcagc gcctcgtatt cgtcgtcggt cagccagaac ttgcgctgac
    16741 gcatcccttt ggccttcatg cgctcggcat atcgcgcttg gcgtacagcg tcagggctgg
    16801 ccagcaggtc gccggtctgc ttgtcctttt ggtctttcat atcagtcacc gagaaacttg
    16861 ccggggccga aaggcttgtc ttcgcggaac aaggacaagg tgcagccgtc aaggttaagg
    16921 ctggccatat cagcgactga aaagcggcca gcctcggcct tgtttgacgt ataaccaaag
    16981 ccaccgggca accaatagcc cttgtcactt ttgatcaggt agaccgaccc tgaagcgctt
    17041 ttttcgtatt ccataaaacc cccttctgtg cgtgagtact catagtataa caggcgtgag
    17101 taccaacgca agcactacat gctgaaatct ggcccgcccc tgtccatgcc tcgctggcgg
    17161 ggtgccggtg cccgtgccag ctcggcccgc gcaagctgga cgctgggcag acccatgacc
    17221 ttgctgacgg tgcgctcgat gtaatccgct tcgtggccgg gcttgcgctc tgccagcgct
    17281 gggctggcct cggccatggc cttgccgatt tcctcggcac tgcggccccg gctggccagc
    17341 ttctgcgcgg cgataaagtc gcacttgctg aggtcatcac cgaagcgctt gaccagcccg
    17401 gccatctcgc tgcggtactc gtccagcgcc gtgcgccggt ggcggctaag ctgccgctcg
    17461 gacagttcga ggctggccag cctgcgggcc ttctcctgct gccgctgggc ctgctcgatc
    17521 tgctggccag cctgctgcac cagcgccggg ccagcggtgg cggtcttgcc cttggattca
    17581 cgcagcagca cccacggctg ataaccggcg cgggtggtgt gcttgtcctt gcggttggtg
    17641 aagcccgcca agcggccata gtggcggctg tcggcgctgg ccgggtcggc gtcgtactcg
    17701 ctggccagcg tccgggcaat ctgcccccga agttcaccgc ctgcggcgtc ggccaccttg
    17761 acccatgcct gatagttctt cgggctggtt tccactacca gggcaggctc ccggccctcg
    17821 gctttcatgt catccaggtc aaactcgctg aggtcgtcca ccagcaccag accatgccgc
    17881 tcctgctcgg cgggcctgat atacacgtca ttgccctggg cattcatccg cttgagccat
    17941 ggcgtgttct ggagcacttc ggcggctgac cattcccggt tcatcatctg gccggtggtg
    18001 gcgtccctga cgccgatatc gaagcgctca cagcccatgg ccttgagctg tcggcctatg
    18061 gcctgcaaag tcctgtcgtt cttcatcggg ccaccaagcg cagccagatc gagccgtcct
    18121 cggttgtcag tggcgtcagg tcgagcaaga gcaacgatgc gatcagcagc accaccgtag
    18181 gcatcatgga agccagcatc acggttagcc atagcttcca gtgccacccc cgcgacgcgc
    18241 tccgggcgct ctgcgcggcg ctgctcacct cggcggctac ctcccgcaac tctttggcca
    18301 gctccaccca tgccgcccct gtctggcgct gggctttcag ccactccgcc gcctgcgcct
    18361 cgctggcctg ctgggtctgg ctcatgacct gccgggcttc gtcggccagt gtcgccatgc
    18421 tctgggccag cggttcgatc tgctccgcta actcgttgat gcctctggat ttcttcactc
    18481 tgtcgattgc gttcatggtc tattgcctcc cggtattcct gtaagtcgat gatctgggcg
    18541 ttggcggtgt cgatgttcag ggccacgtct gcccggtcgg tgcggatgcc ccggccttcc
    18601 atctccacca cgttcggccc caggtgaaca ccgggcaggc gctcgatgcc ctgcgcctca
    18661 agtgttctgt ggtcaatgcg ggcgtcgtgg cctgcccgct ctaatgcccg gttggcatgg
    18721 tcggcccatg cctcgcgggt ctgctcaagc catgccttgg gcttgagcgc ttcggtcttc
    18781 tgtgccccgc ccttctccgg ggtcttgccg ttgtaccgct tgaaccactg agcggcgggc
    18841 cgctcgatgc cgtcattgat ccgctcggag atcatcaggt ggcagtgcgg gttctcgccg
    18901 ccaccggcat ggatggccag cgtatacggc aggcgctcgg caccggtcag gtgctgggcg
    18961 aactcggacg ccagcgcctt ctgctggtcg agggtcagct cgaccggcag ggcaaattcg
    19021 acctccttga acagccgccc attggcgcgt tcatacaggt cggcagcatc ccagtagtcg
    19081 gcgggccgct cgacgaactc cggcatgtgc ccggattcgg cgtgcaagac ttcatccatg
    19141 tcgcgggcat acttgccttc gcgctggatg aagtcggcct tggccctggc cgattggccg
    19201 cccgacctgc tgccggtttt cgccgtaagg tgataaatcg ccatgctgcc tcgctgttgc
    19261 ttttgctttt cggctccatg caatggccct cggagagcgc accgcccgaa gggtggccgt
    19321 taggccagtt tctcgaagag aaaccggtaa gtgcgccctc ccctacaaag tagggtcggg
    19381 attgccgccg ctgtgcctcc atgatagcct acgagacagc acattaacaa tggggtgtca
    19441 agatggttaa ggggagcaac aaggcggcgg atcggctggc caagctcgaa gaacaacgag
    19501 cgcgaatcaa tgccgaaatt cagcgggtgc gggcaaggga acagcagcaa gagcgcaaga
    19561 acgaaacaag gcgcaaggtg ctggtggggg ccatgatttt ggccaaggtg aacagcagcg
    19621 agtggccgga ggatcggctc atggcggcaa tggatgcgta ccttgaacgc gaccacgacc
    19681 gcgccttgtt cggtctgccg ccacgccaga aggatgagcc gggctgaatg atcgaccgag
    19741 acaggccctg cggggctgca cacgcgcccc cacccttcgg gtagggggaa aggccgctaa
    19801 agcggctaaa agcgctccag cgtatttctg cggggtttgg tgtggggttt agcgggcttt
    19861 gcccgccttt ccccctgccg cgcagcggtg gggcggtgtg tagcctagcg cagcgaatag
    19921 accagctatc cggcctctgg ccgggcatat tgggcaaggg cagcagcgcc ccacaagggc
    19981 gctgataacc gcgcctagtg gattattctt agataatcat ggatggattt ttccaacacc
    20041 ccgccagccc ccgcccctgc tgggtttgca ggtttggggg cgtgacagtt attgcagggg
    20101 ttcgtgacag ttattgcagg ggggcgtgac agttattgca ggggttcgtg acagttagta
    20161 cgggagtgac gggcactggc tggcaatgtc tagcaacggc aggcatttcg gctgagggta
    20221 aaagaacttt ccgctaagcg atagactgta tgtaaacaca gtattgcaag gacgcggaac
    20281 atgcctcatg tggcggccct cgcgagaatt aattcagata aaaaaaatcc ttagctttcg
    20341 ctaaggatga tttctagcga tgaccctgct gattggttcg ctgaccgtag actggttggc
    20401 ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct
    20461 cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa
    20521 cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac
    20581 caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgac atcgcgcgcg
    20641 cgcgcgcgga tgctagcggc gcgccaaaaa aaagcccgct catta
//
