LOCUS       sequence-467490-       11725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 
            with a cumate inducible promoter in Gordonibacter urolithinfaciens 
            DSM 27213 for protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11725)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 11725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF26-Pct3-(+)-Eldh1(nHis6)(GpDSM19378)
FEATURES             Location/Qualifiers
     source          1..11725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(1497..2108)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2513..2540
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             2601..2618
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5349..5376
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      5508..5535
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(5967..6818)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(6805..7644)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(8155..9126)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            10365..10452
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      10794..11188
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(11351..11394)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            11490..11599
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctag
     1501 cgcttgaatt tcgcgtaccg ctctcgcgca atttcgagtg tcgagttcct gacacgctca
     1561 aagcgttctt tgtccttctg ccataggcta cgaacagcaa gcccacgcac cgaattgaat
     1621 atcaaccaaa ggatatcttc tgcatcatca cgcgaaagac cacggctcac cagaacacca
     1681 agccacatat cctcgacgac aaaccgattc ctctctaccg tgcgctgaat accctcgcgt
     1741 aacgctgggt cccggtcggc agccacaatc aaatcaaggc tgatagagaa gtcatcgtcg
     1801 aggaaaaatt cggcggcgtc gtccagcatt tgctggatga cgtcatcctc tggcttcaat
     1861 ttcgctaatc gagcccgact gcgttcggtg atctgttcgt aaagccattc aaaagtggca
     1921 agcagaagct caagctttgt cgggaaatga tggctctgcg ctcctctgga gacaccagca
     1981 gcaccgggca catctgcgat ccggaatccc gcgtaacctt tttcccgtaa aacccccagg
     2041 gccgctgcaa tcaacttgcc ctgggtctcc attgcgcgct ctgcctgtgt tcttctcttt
     2101 ggactcatga tcaccagcga cctgctttcg ttcgtccggg catcacccgg gttactatct
     2161 agtatcaata actagattat tggttgaaac gtactatgtc aagcagttct aaagattgac
     2221 tccaaacggt cgccttgcta tgctcgtttc gtttcctccg cacgcgcgcg aaaggctcct
     2281 tcacggagcc aatgtacgca aaaaaccccg cttcagcggg gttttttcgc caaaaaaaac
     2341 cccgccctgt caggggcggg gttttttttt tttagggccc tactgactgt gagcccgctc
     2401 gatcaacgga tcggtaaaat acgggcgaaa aagagaatcc cgagcttttt tgttgccgca
     2461 tagcgtatag taagcatgtg ccctcgaggt gagggcgagt gttgcggcca gtaacaaaca
     2521 gacaatctgg tctgtttgta ttatgcagca cacagtgtta cctcctcggg gacgaggaga
     2581 ggaaagagga tttaaaaatg catcaccatc accatcacga ctacgacatc ccgactacct
     2641 cgaacgatta ctacgaaagc caccgaggcg aatggcagtg gcaggacggc gagtacaccg
     2701 tcacgcgcac gaccgcctgg tcggggcccg gttgccacga cggctgcggc gtgctgtact
     2761 acacgaagga cggcaagctg gagaaggtgg agggcgaccc cgacaacccg ttcaaccagg
     2821 gcgcgctgtg catgcgctgc ctggagatgc ccgagttcgt gaacaacccc acgcgcctcg
     2881 gccaccccat gaagcgtgcc ggcgagcggg gcgagaacaa atgggagcgc gtgtcctggg
     2941 aggaggcgct cgacctcatc gaggaaaagg tacgcgccat ctggaaggac tacggccccg
     3001 agtccatcgt ctgtcatatc ggcaccgggc gcaacaactg cgaccagata ccgtacctgt
     3061 gctacgcggc gttcggatcg ccgaacttct ccatgggcta tctggccggc gactcctgct
     3121 tctgcccgcg ctccgcgtcg atggcgtcgc agaacggcga cttcatgatc gccgactgct
     3181 cgcagcagtt cgagaagcgc tacgacgaga gcacgggctg gcgctgcccc gaagtcatcg
     3241 tgaactgggg ctgcaacgcg gtcgtctcga actccgacaa ctttttcggc ggctggatcg
     3301 tggactgcat gcagcgcggc tccaagatga tcaccatcga cccgtcgctc acctggctgg
     3361 cctcccgctc ggcgtactgg ctgcgcgtgc ggccgggcac cgacgcggct ttggccatgg
     3421 ccatgctcaa cgtcatcatc tccgaggacc tctacgacca cgatttcgtg gccaagtggt
     3481 gctacggctt cgacgagctt gccgagcgcg tgcgcgactg gacgcccgag cgggctgcgg
     3541 aggtttgctg ggtcgaggcc gaggacatcg tgggcgccgc ccgcctgtac gcccaggccg
     3601 accccgccgc catccagtgg gggctcaagc tcgaccagac caccaacgcc accgccaccg
     3661 cgtgcgccgt caactcgctg tgggccatca cgggcaacgt ggacaatccc ggcggcaaca
     3721 tcatcatccg caacgccttc gaccagaacc tctcgtacgg ctacgggtac cacctgctgc
     3781 cgcccgaggt gcaggcgaag cgcctggggg ccgagttccc gctgatgagc cgtgcgggct
     3841 acagctcctc cgcccatgcg gactccgtgc tgaaggccat cgagaccggc gagccgtatc
     3901 ctatccgcat ggtgtggatg acgtccacga accccatcgc caacatgggc gccgacgcgc
     3961 cgcgcctgtt ccgcgccttg aagagcgtcg atttcgtggt ggtgaacgac ctgttcatga
     4021 cgccaaccgc catcgccttc gccgatctgg tgctgcccgc cgccatgtct cccgaacgca
     4081 actcgcagcg ctgctggtgg gttccgaacc gcacgctcac caaggtcacc cagtacgagg
     4141 agtgcgtttc ggacgacgac ctggtcacca tggtgggcaa acgcctgcac cccgagaact
     4201 tcccctggga cgacaacgtg ggatggatca accacatctt cgagcacgag acgaacgacg
     4261 agctgcccta caagagcttc gaggaggcgc agaaggtcgt gtggtcctac ccggcgttca
     4321 cctacctcaa gcacgagaag ggcctgctgc gccccgacgg gcagcccggg ttcaacacgc
     4381 cgtccggtcg catcgagctg tacaacacga acctcatgct gtggggctac gatccgctgc
     4441 ccgacttcaa ggagcccagc tgctctccgg tgagcagccc cgagctcgcc gagaagtacc
     4501 cgctcgtcct gaccaccggc gcgcggtcct tcgagttctt ccattccgag catcgccagg
     4561 agggcacgac gtcgcgcgag ctgcaccccg acccgctgtt cgagatgagc cctgccgccg
     4621 ccgaggccgc gggcctctcc gagggcgatt gggcctggat cgagaaccag cgcggccgct
     4681 gccgccagaa gctgcacgtg aacccctcgc tcgacgaccg cgtggtgcgc gccgagcacg
     4741 gctggtggtt ccccgagacc gagggggcgg agccgaacct gtacggcgtg ttcgactcca
     4801 acatcaacaa cctcaccccg cagtgcgaga acggcgacac cggcttcggc gccccgtacg
     4861 cgaaccagct gtgccgcgtg tacccgtgca ctgaggagaa cagccgtgtc ctgccgggcg
     4921 agcaggtcac caggcgcggg ggctattcca aggtaatcga cgagtccaac ggcgacgtga
     4981 aggtgggtgc tatctatggc taacgcattg atgatcgact acgagttctg cacgggctgc
     5041 cacgcctgcg aggtggcctg caagaagcat ctgggcctgc ccgcgggcca gcacggcatc
     5101 cgcgtgctcc aggacggccc ccgcgagctc gcgggcggag ggtgggagta caacttcctg
     5161 cccatgccca cgtcgctctg cgacctgtgc gcctcccgcc tggccgaggg caaggacgcc
     5221 gcctgcgtcc accactgccc ggcccactgc atggcgctcg ggccggccga ggagctggcg
     5281 gcacggttcg acgggaaggt caaccgcgca ctgttcgttc ccgcgcagta aactagtcga
     5341 ctcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag agagaatata
     5401 aaaagccaga ttattaatcc ggctttttta ttatttaatt cctgttataa aaaaaggatc
     5461 aattttgaac tctctcccaa agttgatccc ttaacgatcc ctgcaggaga aggccatcct
     5521 gacggatggc ctttttgaag ctttctagaa tcgggggccc ccggggggat tctagaacaa
     5581 tggaagtcct cgtctcgccc tcgaattagc ccgcttaatg agcgggcttt ttttgaatta
     5641 attctcgcga gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     5701 attaatgtaa gttagcgcga attgcaagct ggccgacgcg ctgggctacg tcttgctggc
     5761 gttcgggagc agaagagcat acatctggaa gcaaagccag gaaagcggcc tatggagctg
     5821 tgcggcagcg ctcagtaggc aatttttcaa aatattgtta agccttttct gagcatggta
     5881 tttttcatgg tattaccaat tagcaggaaa ataagccatt gaatataaaa gataaaaatg
     5941 tcttgtttac aatagagtgg ggggggtcag cctgccgcct tgggccgggt gatgtcgtac
     6001 ttgcccgccg cgaactcggt taccgtccag cccagcgcga ccagctccgg caacgcctcg
     6061 cgcacccgct ggcggcgctt gcgcatggtc gaaccactgg cctctgacgg ccagacatag
     6121 ccgcacaagg tatctatgga agccttgccg gttttgccgg ggtcgatcca gccacacagc
     6181 cgctggtgca gcaggcgggc ggtttcgctg tccagcgccc gcacctcgtc catgctgatg
     6241 cgcacatgct ggccgccacc catgacggcc tgcgcgatca aggggttcag ggccacgtac
     6301 aggcgcccgt ccgcctcgtc gctggcgtac tccgacagca gccgaaaccc ctgccgcttg
     6361 cggccattct gggcgatgat ggataccttc caaaggcgct cgatgcagtc ctgtatgtgc
     6421 ttgagcgccc caccactatc gacctctgcc ccgatttcct ttgccagcgc ccgatagcta
     6481 cctttgacca ccatggcatc agcggtgacg gcctcccact tgggttccag gaacagccgg
     6541 agctgccgtc cgccttcggt cttgggttcc gggccaagca ctaggccatt aggcccagcc
     6601 atggccacca gcccttgcag gatgcgcaga tcatcagcgc ccagcggctc cgggccgctg
     6661 aactcgatcc gcttgccgtc gccgtagtca tacgtcacgt ccagcttgct gcgcttgcgc
     6721 tcgccccgct tgagggcacg gaacaggccg ggggccagac agtgcgccgg gtcgtgtcgg
     6781 acgtggctga ggctgtgctt gttcttaggc ttcaccacgg ggcaccccct tgctcttgcg
     6841 ctgcctctcc agcacggcgg gcttgagcac cccgccgtca tgccgcctga accaccgatc
     6901 agcgaacggt gcgccatagt tggccttgct cacaccgaag cggacgaaga accggcgctg
     6961 gtcgtcgtcc acaccccatt cctcggcctc ggcgctggtc atgctcgaca ggtaggactg
     7021 ccagcggatg ttatcgacca gtaccgagct gccccggctg gcctgctgct ggtcgcctgc
     7081 gcccatcatg gccgcgccct tgctggcatg gtgcaggaac acgatagagc acccggtatc
     7141 ggcggcgatg gcctccatgc gaccgatgac ctgggccatg gggccgctgg cgttttcttc
     7201 ctcgatgtgg aaccggcgca gcgtgtccag caccatcagg cggcggccct cggcggcgcg
     7261 cttgaggccg tcgaaccact ccggggccat gatgttgggc aggctgccga tcagcggctg
     7321 gatcagcagg ccgtcagcca cggcttgccg ttcctcggcg ctgaggtgcg ccccaagggc
     7381 gtgcaggcgg tgatgaatgg cggtgggcgg gtcttcggcg ggcaggtaga tcaccgggcc
     7441 ggtgggcagt tcgcccacct ccagcagatc cggcccgcct gcaatctgtg cggccagttg
     7501 cagggccagc atggatttac cggcaccacc gggcgacacc agcgccccga ccgtaccggc
     7561 caccatgttg ggcaaaacgt agtccagcgg tggcggcgct gctgcgaacg cctccagaat
     7621 attgataggc ttatgggtag ccattgattg cctcctttgc aggcagttgg tggttaggcg
     7681 ctggcggggt cactaccccc gccctgcgcc gctctgagtt cttccaggca ctcgcgcagc
     7741 gcctcgtatt cgtcgtcggt cagccagaac ttgcgctgac gcatcccttt ggccttcatg
     7801 cgctcggcat atcgcgcttg gcgtacagcg tcagggctgg ccagcaggtc gccggtctgc
     7861 ttgtcctttt ggtctttcat atcagtcacc gagaaacttg ccggggccga aaggcttgtc
     7921 ttcgcggaac aaggacaagg tgcagccgtc aaggttaagg ctggccatat cagcgactga
     7981 aaagcggcca gcctcggcct tgtttgacgt ataaccaaag ccaccgggca accaatagcc
     8041 cttgtcactt ttgatcaggt agaccgaccc tgaagcgctt ttttcgtatt ccataaaacc
     8101 cccttctgtg cgtgagtact catagtataa caggcgtgag taccaacgca agcactacat
     8161 gctgaaatct ggcccgcccc tgtccatgcc tcgctggcgg ggtgccggtg cccgtgccag
     8221 ctcggcccgc gcaagctgga cgctgggcag acccatgacc ttgctgacgg tgcgctcgat
     8281 gtaatccgct tcgtggccgg gcttgcgctc tgccagcgct gggctggcct cggccatggc
     8341 cttgccgatt tcctcggcac tgcggccccg gctggccagc ttctgcgcgg cgataaagtc
     8401 gcacttgctg aggtcatcac cgaagcgctt gaccagcccg gccatctcgc tgcggtactc
     8461 gtccagcgcc gtgcgccggt ggcggctaag ctgccgctcg gacagttcga ggctggccag
     8521 cctgcgggcc ttctcctgct gccgctgggc ctgctcgatc tgctggccag cctgctgcac
     8581 cagcgccggg ccagcggtgg cggtcttgcc cttggattca cgcagcagca cccacggctg
     8641 ataaccggcg cgggtggtgt gcttgtcctt gcggttggtg aagcccgcca agcggccata
     8701 gtggcggctg tcggcgctgg ccgggtcggc gtcgtactcg ctggccagcg tccgggcaat
     8761 ctgcccccga agttcaccgc ctgcggcgtc ggccaccttg acccatgcct gatagttctt
     8821 cgggctggtt tccactacca gggcaggctc ccggccctcg gctttcatgt catccaggtc
     8881 aaactcgctg aggtcgtcca ccagcaccag accatgccgc tcctgctcgg cgggcctgat
     8941 atacacgtca ttgccctggg cattcatccg cttgagccat ggcgtgttct ggagcacttc
     9001 ggcggctgac cattcccggt tcatcatctg gccggtggtg gcgtccctga cgccgatatc
     9061 gaagcgctca cagcccatgg ccttgagctg tcggcctatg gcctgcaaag tcctgtcgtt
     9121 cttcatcggg ccaccaagcg cagccagatc gagccgtcct cggttgtcag tggcgtcagg
     9181 tcgagcaaga gcaacgatgc gatcagcagc accaccgtag gcatcatgga agccagcatc
     9241 acggttagcc atagcttcca gtgccacccc cgcgacgcgc tccgggcgct ctgcgcggcg
     9301 ctgctcacct cggcggctac ctcccgcaac tctttggcca gctccaccca tgccgcccct
     9361 gtctggcgct gggctttcag ccactccgcc gcctgcgcct cgctggcctg ctgggtctgg
     9421 ctcatgacct gccgggcttc gtcggccagt gtcgccatgc tctgggccag cggttcgatc
     9481 tgctccgcta actcgttgat gcctctggat ttcttcactc tgtcgattgc gttcatggtc
     9541 tattgcctcc cggtattcct gtaagtcgat gatctgggcg ttggcggtgt cgatgttcag
     9601 ggccacgtct gcccggtcgg tgcggatgcc ccggccttcc atctccacca cgttcggccc
     9661 caggtgaaca ccgggcaggc gctcgatgcc ctgcgcctca agtgttctgt ggtcaatgcg
     9721 ggcgtcgtgg cctgcccgct ctaatgcccg gttggcatgg tcggcccatg cctcgcgggt
     9781 ctgctcaagc catgccttgg gcttgagcgc ttcggtcttc tgtgccccgc ccttctccgg
     9841 ggtcttgccg ttgtaccgct tgaaccactg agcggcgggc cgctcgatgc cgtcattgat
     9901 ccgctcggag atcatcaggt ggcagtgcgg gttctcgccg ccaccggcat ggatggccag
     9961 cgtatacggc aggcgctcgg caccggtcag gtgctgggcg aactcggacg ccagcgcctt
    10021 ctgctggtcg agggtcagct cgaccggcag ggcaaattcg acctccttga acagccgccc
    10081 attggcgcgt tcatacaggt cggcagcatc ccagtagtcg gcgggccgct cgacgaactc
    10141 cggcatgtgc ccggattcgg cgtgcaagac ttcatccatg tcgcgggcat acttgccttc
    10201 gcgctggatg aagtcggcct tggccctggc cgattggccg cccgacctgc tgccggtttt
    10261 cgccgtaagg tgataaatcg ccatgctgcc tcgctgttgc ttttgctttt cggctccatg
    10321 caatggccct cggagagcgc accgcccgaa gggtggccgt taggccagtt tctcgaagag
    10381 aaaccggtaa gtgcgccctc ccctacaaag tagggtcggg attgccgccg ctgtgcctcc
    10441 atgatagcct acgagacagc acattaacaa tggggtgtca agatggttaa ggggagcaac
    10501 aaggcggcgg atcggctggc caagctcgaa gaacaacgag cgcgaatcaa tgccgaaatt
    10561 cagcgggtgc gggcaaggga acagcagcaa gagcgcaaga acgaaacaag gcgcaaggtg
    10621 ctggtggggg ccatgatttt ggccaaggtg aacagcagcg agtggccgga ggatcggctc
    10681 atggcggcaa tggatgcgta ccttgaacgc gaccacgacc gcgccttgtt cggtctgccg
    10741 ccacgccaga aggatgagcc gggctgaatg atcgaccgag acaggccctg cggggctgca
    10801 cacgcgcccc cacccttcgg gtagggggaa aggccgctaa agcggctaaa agcgctccag
    10861 cgtatttctg cggggtttgg tgtggggttt agcgggcttt gcccgccttt ccccctgccg
    10921 cgcagcggtg gggcggtgtg tagcctagcg cagcgaatag accagctatc cggcctctgg
    10981 ccgggcatat tgggcaaggg cagcagcgcc ccacaagggc gctgataacc gcgcctagtg
    11041 gattattctt agataatcat ggatggattt ttccaacacc ccgccagccc ccgcccctgc
    11101 tgggtttgca ggtttggggg cgtgacagtt attgcagggg ttcgtgacag ttattgcagg
    11161 ggggcgtgac agttattgca ggggttcgtg acagttagta cgggagtgac gggcactggc
    11221 tggcaatgtc tagcaacggc aggcatttcg gctgagggta aaagaacttt ccgctaagcg
    11281 atagactgta tgtaaacaca gtattgcaag gacgcggaac atgcctcatg tggcggccct
    11341 cgcgagaatt aattcagata aaaaaaatcc ttagctttcg ctaaggatga tttctagcga
    11401 tgaccctgct gattggttcg ctgaccgtag actggttggc ttggtttcat cagccatccg
    11461 cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag gattcccgtt
    11521 gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc
    11581 tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac
    11641 cctttggcaa aatcctgtat atcgtgcgac atcgcgcgcg cgcgcgcgga tgctagcggc
    11701 gcgccaaaaa aaagcccgct catta
//
