LOCUS       sequence-467495-       13690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate 
            inducible promoter, without accessory genes (inactive), in 
            Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13690)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 13690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80EN8-Pct3-(+)-Eddh(cHis6)-noAccs(ElA2)
FEATURES             Location/Qualifiers
     source          1..13690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(1497..2108)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2512..2539
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             5701..5718
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      7314..7341
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      7473..7500
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(7932..8783)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(8770..9609)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(10120..11091)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            12330..12417
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      12759..13153
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(13316..13359)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            13455..13564
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctag
     1501 cgcttgaatt tcgcgtaccg ctctcgcgca atttcgagtg tcgagttcct gacacgctca
     1561 aagcgttctt tgtccttctg ccataggcta cgaacagcaa gcccacgcac cgaattgaat
     1621 atcaaccaaa ggatatcttc tgcatcatca cgcgaaagac cacggctcac cagaacacca
     1681 agccacatat cctcgacgac aaaccgattc ctctctaccg tgcgctgaat accctcgcgt
     1741 aacgctgggt cccggtcggc agccacaatc aaatcaaggc tgatagagaa gtcatcgtcg
     1801 aggaaaaatt cggcggcgtc gtccagcatt tgctggatga cgtcatcctc tggcttcaat
     1861 ttcgctaatc gagcccgact gcgttcggtg atctgttcgt aaagccattc aaaagtggca
     1921 agcagaagct caagctttgt cgggaaatga tggctctgcg ctcctctgga gacaccagca
     1981 gcaccgggca catctgcgat ccggaatccc gcgtaacctt tttcccgtaa aacccccagg
     2041 gccgctgcaa tcaacttgcc ctgggtctcc attgcgcgct ctgcctgtgt tcttctcttt
     2101 ggactcatga tcaccagcga cctgctttcg ttcgtccggg catcacccgg gttactatct
     2161 agtatcaata actagattat tggttgaaac gtactatgtc aagcagttct aaagattgac
     2221 tccaaacggt cgccttgcta tgctcgtttc gtttcctccg cacgcgcgcg aaaggctcct
     2281 tcacggagcc aatgtacgca aaaaaccccg cttcagcggg gttttttcgc caaaaaaaac
     2341 cccgccctgt caggggcggg gttttttttt ttagggccct actgactgtg agcccgctcg
     2401 atcaacggat cggtaaaata cgggcgaaaa agagaatccc gagctttttt gttgccgcat
     2461 agcgtatagt aagcatgtgc cctcgaggtg agggcgagtg ttgcggccag taacaaacag
     2521 acaatctggt ctgtttgtat tatgcagcac acagtgttac ctcctcgggg acgaggagag
     2581 gaaagaggat ttaaaatggg aaagctcaat ctgacgcgcc gtgcgttcac gaagctcacg
     2641 gccgtgacgg gtgcggcgtt ggcctgcgcg gcagcggtcg ccccgaacgc ggcgctggcc
     2701 gaagatgccg gagcggccgc gcgcggcgac gacgtgaaac gcgtgcgcac gtgctgccgc
     2761 ggctgcggaa agatggagtg cggcgtgtgg gtgaccgtgc aaaacggccg cgctatcaag
     2821 gtggagggcg accagtcgtc attccagtcg agcggcaact gctgcggcaa gtcgcagtcg
     2881 tccatccagg cggcgtacca ccccgaccgc atctaccatc ccatgaagcg cacgaatccc
     2941 aagggcgagg atcccggctg gcagcgcatc acgtgggacg aggcgatgga gatcgtcggc
     3001 accaagttca acgagctgat ggaccgctac ggcggacagt gcatcttcaa catggcgggc
     3061 acgtcgcgcc agtgggtgta cgggccctac gcgttctaca agtggctgtt cgacacgccg
     3121 aacgcgcacg tggcgtcgga aatctgcaag gggccgcgcc gtttgatggg gtggatcagc
     3181 tcggtcgacg gcgcgccgtg gatggcgctg cgcgacgggc cgcgcgtgta cgtgcagtgg
     3241 gggaccgcgc ccgaaaactc gaactacgat gacagctgcc gcaacctcgt ggacaagatg
     3301 accgaggccg acgtgcacat ctgcatcgat ccgcgcctgt ccggctcggg caaggaggcc
     3361 gactactggc tgaacctgcg tcccggctcc gacggcgcgc tggcgctgtg ctggcagcac
     3421 ctcgtcatca agaacgacct ggtggactgg gagttcgtga agcgctggac gaactcgtcg
     3481 ttcctcgtgg tggaggaccg ggagcccacg ggcggccgct acatcgacct gtccacgccg
     3541 ctcaacaacg ccggcatccc cgccgacgtc gtgggcacga agctcaagac gcgcctgctg
     3601 aaggaaagcg atgtggtgga gggcggcagc ccgcgcaagt tctacgcgtg gaacaaactg
     3661 gccaacgacg gcgccggcgg cttggtgatg tgggacgtcg acaccacgca atgggagggc
     3721 tgcaaccacg tggcccccac gcgcgaccag atggaggtcg tgtacaaggg cacgtcgcag
     3781 gagggctatt tgccgccgct gtcctatcac gagctggagg aagccggcat cgacctggat
     3841 atgcgtggga cgcatgaggt ggagctgctc gacggttcga agcacacggc gaagccggtg
     3901 tgggcgtacc tcgaggaatc ggtggcggac tgcacgcccg cgtggtgctc cgagatcacg
     3961 ggcctcgatc ctgcgctcat cgaagaggcg tgcctcgtgt gggccacgcg tcccgagggg
     4021 caggattacg gcaacggcgg tatccacctg aacctcgcgc ccgaccagat cggcaactgc
     4081 acgcagacgg tgcgcgcggt gctgcacctc atctacatga ccggcaactt cgacacgccc
     4141 gccggcaacc gcggcctcac gcgctcgccc atcgacgagc aggccacggc cgcgcccggc
     4201 tcgaacatgc cgcaggaagt gaaggcccag ctgatcgcct tgggcgagat ccccgtggaa
     4261 ggcgtcacgc ccgatcccct caacgtgccc gaccgctacg acacgctgtc gaacatggtg
     4321 ggcgccgacg agttccccat cacggcgtac tacaacgagt gggccgacgc gacgcgcatc
     4381 tgggacgcct gcctcaccgg cgagccgtac cccgtgcgcg gcggcatcaa cgagtccggc
     4441 tcgttcatga acatgtcgaa cgcgaacctg gcctgggagg cgttgcagtc gctcgatttc
     4501 tgggtggaca tcaacatgtt ccaccatccc ggcaccgaga tggccgacat cctgctgccg
     4561 tgccagcatt ggctggagat caacaacatc cgcgtgtcgc agggcgcgtc cggcggtatc
     4621 ggcgccacca tccgcgcggt cgagccgccc agcgacacga agttcgacta cgacatcaac
     4681 cgcctgctgt tcgacgccgt gggcggcccg aacggaacct ggaccaacat cgcgggcgac
     4741 gcgcccggcg gctaccacgt ggacgagcgc ttggaggact ggttccagaa caactcgaag
     4801 accaatccca aggtgaaatg gcagcattgg gacgacttcg tggaggactt tcaggagaac
     4861 ggctggatca acgccaagga gatcgagccc gaccgctggg gcacgtaccg ccgcttcgag
     4921 accggctgga tgcgcatcgg caaggacgcg tgcaccggct ccacgttcag cgcggcgttc
     4981 gacgacgccg gcaatccggt gaacaacttc ggctgcccca cgccgacggg cctcgtggaa
     5041 ttttggccgc tcgtgttcga gacgtactgc gtggacaagg cgaacgagtt caaccccggc
     5101 aagttcgacc tggtgcacga gatgatgccg cactacgacg agccgaaatc gggccccaag
     5161 ggcgacgtgg acatgaacga gtatcccatt atcctcacca ccggccgccg catacccgtg
     5221 tacttccatt ccgagcaccg gcagctgccg tggtgtcgcg agctgtggcc ggcaccgcgt
     5281 ttggaggtga accccgaaga cgctgccgag ctggggctcg agcagggcga ttgggcctgg
     5341 atcgagacgg agtggggcaa ggtgcgccag tgcgtcgatt tgtactacgg catcgcgaag
     5401 gggtgggcga acgccgagca cgcctggtgg ttccccgagc tgcccgcgcc gacgcacggg
     5461 ttcgagctgt cgaacatcga gtgcatctgg aacccctacg gccaggatcc gttcatcggg
     5521 tcgtcccaca tgcgcggcgt gcccgtgaag atatacaagg ccacgcccga gaactgcccc
     5581 gacggcaagg tcatcccctg cgcgcccgag gacggcaccg agatcatcta cgatgcctcc
     5641 gacccgcgcc tgaaggaatg gctgccgaac tacaccatcc gagaggaggc gggcagcagc
     5701 catcaccatc accatcacta aagccatgac ccagtacgcg atcgtcaccg atttgaaccg
     5761 gtgcgtcggc tgcctggcct gcatggttgc ctgcaaggcg gtcaacaacg tgccggtggg
     5821 caactattgg aacaaggtgc tgcgcatcgg cccctcgctg aaagaggggg ccacgtcgtc
     5881 gcacgacgtg gagatgtact acctgcccgt gcagtgccag cactgcgccg accccgagtg
     5941 cgtgaaggtg tgcccgacgg gcgcctcgca caaactggag gacggcacgg tgcagatcga
     6001 caaggcgaag tgcatcggct gccagttctg cgcgatgtcg tgcccctaca acgtgcggta
     6061 cctgaacgag gaagagggcg tcgtggagaa gtgcacgctg tgcgcgcaga tcgtggggca
     6121 gggcgggttg ccgcaatgcg tcatccagtg cggagggcgc gcccggttct tcggcgatct
     6181 ggaccagggg atcgagtcgt tcgaagcccc cgccgtcggc tacgacaccg atcgcaccta
     6241 cgacggcctg tatgcaggca accgcgtgac gctgggcgag ttggcgcagg acttcgatgc
     6301 gagcaccgat ctgcatcatc tgccgaacgt cggcaacgat ccgaacttcg cgttcatcct
     6361 gcgcaaccgc gactggaagg gtgatgaata atggaactcc agtggccttt gattttgttc
     6421 accacgctgg ttgcgtggag cgcggggctg ttcgggacgc aggcgctgat ggcggtgttc
     6481 ggcgtcggga agaaggcgca ggtgccggcg tgggttgcgt cggccgtctt gctggcggcc
     6541 ggcgggatcg cggtgttctt ccaccttgag cactgggagc ggatcttcaa cgggttcggg
     6601 catttgacga gcgggatcac gcaggagctg atcgcgatcg tggtgcttgc cgtggccgcc
     6661 gtggcctacc tcgtgctcat gcgcaagtcc gacgacggcg cgagcgtgcc gaagtggctg
     6721 gcgtggctgt ccgtcgcctt gtcggtcgtg ctggtcgccg tcatggcgca cagctacacg
     6781 atggccgccc gccccgcctg ggactcggcc ctctggatcc tctacgtcct cggcaacgcc
     6841 tgcgtgttgg gccccgcgac gttcgccctc ctctccacct tggctgcagg gggaccccgg
     6901 gaccaacccg ccgatcgcgc agccgacgcc ggggcccccg caggtaggac ggccctcgcc
     6961 ggcgccgcca tcaacgccct cgcaaccctc gccttcgccg ccttcctcca gctctccgcc
     7021 ggctccttcg ccgacgtcgg cctctacttc gacccgacgc accccaccaa ggccatggcc
     7081 gacgcagccg cgacggtcgc ctcccaggcc ccgctgctgt ggctgggagc cgtggccgtg
     7141 ggcgcgatcg tgcccctggc cgccgccttc atgggcaggc gcacggggaa ctggaggctc
     7201 tgggccccgg tcgcgatagc cgccgccctc gtcggcgccg tctgcatgcg cgccgtcttc
     7261 tacaacctgg ggctcagcgt gttcatgttc tactagacta gtcgactcac actggctcac
     7321 cttcgggtgg gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt
     7381 aatccggctt ttttattatt taattcctgt tataaaaaaa ggatcaattt tgaactctct
     7441 cccaaagttg atcccttaac gatccctgca ggagaaggcc atcctgacgg atggcctttt
     7501 tgaagctttc tagaatcggg ggcccccggg gggattctag aacaatggaa gtcctcgtct
     7561 cgccctcgaa ttagcccgct taatgagcgg gctttttttg aattaattct cgcgagctgg
     7621 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtaagttag
     7681 cgcgaattgc aagctggccg acgcgctggg ctacgtcttg ctggcgttcg ggagcagaag
     7741 agcatacatc tggaagcaaa gccaggaaag cggcctatgg agctgtgcgg cagcgctcag
     7801 taggcaattt ttcaaaatat tgttaagcct tttctgagca tggtattttt catggtatta
     7861 ccaattagca ggaaaataag ccattgaata taaaagataa aaatgtcttg tttacaatag
     7921 agtggggggg gtcagcctgc cgccttgggc cgggtgatgt cgtacttgcc cgccgcgaac
     7981 tcggttaccg tccagcccag cgcgaccagc tccggcaacg cctcgcgcac ccgctggcgg
     8041 cgcttgcgca tggtcgaacc actggcctct gacggccaga catagccgca caaggtatct
     8101 atggaagcct tgccggtttt gccggggtcg atccagccac acagccgctg gtgcagcagg
     8161 cgggcggttt cgctgtccag cgcccgcacc tcgtccatgc tgatgcgcac atgctggccg
     8221 ccacccatga cggcctgcgc gatcaagggg ttcagggcca cgtacaggcg cccgtccgcc
     8281 tcgtcgctgg cgtactccga cagcagccga aacccctgcc gcttgcggcc attctgggcg
     8341 atgatggata ccttccaaag gcgctcgatg cagtcctgta tgtgcttgag cgccccacca
     8401 ctatcgacct ctgccccgat ttcctttgcc agcgcccgat agctaccttt gaccaccatg
     8461 gcatcagcgg tgacggcctc ccacttgggt tccaggaaca gccggagctg ccgtccgcct
     8521 tcggtcttgg gttccgggcc aagcactagg ccattaggcc cagccatggc caccagccct
     8581 tgcaggatgc gcagatcatc agcgcccagc ggctccgggc cgctgaactc gatccgcttg
     8641 ccgtcgccgt agtcatacgt cacgtccagc ttgctgcgct tgcgctcgcc ccgcttgagg
     8701 gcacggaaca ggccgggggc cagacagtgc gccgggtcgt gtcggacgtg gctgaggctg
     8761 tgcttgttct taggcttcac cacggggcac ccccttgctc ttgcgctgcc tctccagcac
     8821 ggcgggcttg agcaccccgc cgtcatgccg cctgaaccac cgatcagcga acggtgcgcc
     8881 atagttggcc ttgctcacac cgaagcggac gaagaaccgg cgctggtcgt cgtccacacc
     8941 ccattcctcg gcctcggcgc tggtcatgct cgacaggtag gactgccagc ggatgttatc
     9001 gaccagtacc gagctgcccc ggctggcctg ctgctggtcg cctgcgccca tcatggccgc
     9061 gcccttgctg gcatggtgca ggaacacgat agagcacccg gtatcggcgg cgatggcctc
     9121 catgcgaccg atgacctggg ccatggggcc gctggcgttt tcttcctcga tgtggaaccg
     9181 gcgcagcgtg tccagcacca tcaggcggcg gccctcggcg gcgcgcttga ggccgtcgaa
     9241 ccactccggg gccatgatgt tgggcaggct gccgatcagc ggctggatca gcaggccgtc
     9301 agccacggct tgccgttcct cggcgctgag gtgcgcccca agggcgtgca ggcggtgatg
     9361 aatggcggtg ggcgggtctt cggcgggcag gtagatcacc gggccggtgg gcagttcgcc
     9421 cacctccagc agatccggcc cgcctgcaat ctgtgcggcc agttgcaggg ccagcatgga
     9481 tttaccggca ccaccgggcg acaccagcgc cccgaccgta ccggccacca tgttgggcaa
     9541 aacgtagtcc agcggtggcg gcgctgctgc gaacgcctcc agaatattga taggcttatg
     9601 ggtagccatt gattgcctcc tttgcaggca gttggtggtt aggcgctggc ggggtcacta
     9661 cccccgccct gcgccgctct gagttcttcc aggcactcgc gcagcgcctc gtattcgtcg
     9721 tcggtcagcc agaacttgcg ctgacgcatc cctttggcct tcatgcgctc ggcatatcgc
     9781 gcttggcgta cagcgtcagg gctggccagc aggtcgccgg tctgcttgtc cttttggtct
     9841 ttcatatcag tcaccgagaa acttgccggg gccgaaaggc ttgtcttcgc ggaacaagga
     9901 caaggtgcag ccgtcaaggt taaggctggc catatcagcg actgaaaagc ggccagcctc
     9961 ggccttgttt gacgtataac caaagccacc gggcaaccaa tagcccttgt cacttttgat
    10021 caggtagacc gaccctgaag cgcttttttc gtattccata aaaccccctt ctgtgcgtga
    10081 gtactcatag tataacaggc gtgagtacca acgcaagcac tacatgctga aatctggccc
    10141 gcccctgtcc atgcctcgct ggcggggtgc cggtgcccgt gccagctcgg cccgcgcaag
    10201 ctggacgctg ggcagaccca tgaccttgct gacggtgcgc tcgatgtaat ccgcttcgtg
    10261 gccgggcttg cgctctgcca gcgctgggct ggcctcggcc atggccttgc cgatttcctc
    10321 ggcactgcgg ccccggctgg ccagcttctg cgcggcgata aagtcgcact tgctgaggtc
    10381 atcaccgaag cgcttgacca gcccggccat ctcgctgcgg tactcgtcca gcgccgtgcg
    10441 ccggtggcgg ctaagctgcc gctcggacag ttcgaggctg gccagcctgc gggccttctc
    10501 ctgctgccgc tgggcctgct cgatctgctg gccagcctgc tgcaccagcg ccgggccagc
    10561 ggtggcggtc ttgcccttgg attcacgcag cagcacccac ggctgataac cggcgcgggt
    10621 ggtgtgcttg tccttgcggt tggtgaagcc cgccaagcgg ccatagtggc ggctgtcggc
    10681 gctggccggg tcggcgtcgt actcgctggc cagcgtccgg gcaatctgcc cccgaagttc
    10741 accgcctgcg gcgtcggcca ccttgaccca tgcctgatag ttcttcgggc tggtttccac
    10801 taccagggca ggctcccggc cctcggcttt catgtcatcc aggtcaaact cgctgaggtc
    10861 gtccaccagc accagaccat gccgctcctg ctcggcgggc ctgatataca cgtcattgcc
    10921 ctgggcattc atccgcttga gccatggcgt gttctggagc acttcggcgg ctgaccattc
    10981 ccggttcatc atctggccgg tggtggcgtc cctgacgccg atatcgaagc gctcacagcc
    11041 catggccttg agctgtcggc ctatggcctg caaagtcctg tcgttcttca tcgggccacc
    11101 aagcgcagcc agatcgagcc gtcctcggtt gtcagtggcg tcaggtcgag caagagcaac
    11161 gatgcgatca gcagcaccac cgtaggcatc atggaagcca gcatcacggt tagccatagc
    11221 ttccagtgcc acccccgcga cgcgctccgg gcgctctgcg cggcgctgct cacctcggcg
    11281 gctacctccc gcaactcttt ggccagctcc acccatgccg cccctgtctg gcgctgggct
    11341 ttcagccact ccgccgcctg cgcctcgctg gcctgctggg tctggctcat gacctgccgg
    11401 gcttcgtcgg ccagtgtcgc catgctctgg gccagcggtt cgatctgctc cgctaactcg
    11461 ttgatgcctc tggatttctt cactctgtcg attgcgttca tggtctattg cctcccggta
    11521 ttcctgtaag tcgatgatct gggcgttggc ggtgtcgatg ttcagggcca cgtctgcccg
    11581 gtcggtgcgg atgccccggc cttccatctc caccacgttc ggccccaggt gaacaccggg
    11641 caggcgctcg atgccctgcg cctcaagtgt tctgtggtca atgcgggcgt cgtggcctgc
    11701 ccgctctaat gcccggttgg catggtcggc ccatgcctcg cgggtctgct caagccatgc
    11761 cttgggcttg agcgcttcgg tcttctgtgc cccgcccttc tccggggtct tgccgttgta
    11821 ccgcttgaac cactgagcgg cgggccgctc gatgccgtca ttgatccgct cggagatcat
    11881 caggtggcag tgcgggttct cgccgccacc ggcatggatg gccagcgtat acggcaggcg
    11941 ctcggcaccg gtcaggtgct gggcgaactc ggacgccagc gccttctgct ggtcgagggt
    12001 cagctcgacc ggcagggcaa attcgacctc cttgaacagc cgcccattgg cgcgttcata
    12061 caggtcggca gcatcccagt agtcggcggg ccgctcgacg aactccggca tgtgcccgga
    12121 ttcggcgtgc aagacttcat ccatgtcgcg ggcatacttg ccttcgcgct ggatgaagtc
    12181 ggccttggcc ctggccgatt ggccgcccga cctgctgccg gttttcgccg taaggtgata
    12241 aatcgccatg ctgcctcgct gttgcttttg cttttcggct ccatgcaatg gccctcggag
    12301 agcgcaccgc ccgaagggtg gccgttaggc cagtttctcg aagagaaacc ggtaagtgcg
    12361 ccctccccta caaagtaggg tcgggattgc cgccgctgtg cctccatgat agcctacgag
    12421 acagcacatt aacaatgggg tgtcaagatg gttaagggga gcaacaaggc ggcggatcgg
    12481 ctggccaagc tcgaagaaca acgagcgcga atcaatgccg aaattcagcg ggtgcgggca
    12541 agggaacagc agcaagagcg caagaacgaa acaaggcgca aggtgctggt gggggccatg
    12601 attttggcca aggtgaacag cagcgagtgg ccggaggatc ggctcatggc ggcaatggat
    12661 gcgtaccttg aacgcgacca cgaccgcgcc ttgttcggtc tgccgccacg ccagaaggat
    12721 gagccgggct gaatgatcga ccgagacagg ccctgcgggg ctgcacacgc gcccccaccc
    12781 ttcgggtagg gggaaaggcc gctaaagcgg ctaaaagcgc tccagcgtat ttctgcgggg
    12841 tttggtgtgg ggtttagcgg gctttgcccg cctttccccc tgccgcgcag cggtggggcg
    12901 gtgtgtagcc tagcgcagcg aatagaccag ctatccggcc tctggccggg catattgggc
    12961 aagggcagca gcgccccaca agggcgctga taaccgcgcc tagtggatta ttcttagata
    13021 atcatggatg gatttttcca acaccccgcc agcccccgcc cctgctgggt ttgcaggttt
    13081 gggggcgtga cagttattgc aggggttcgt gacagttatt gcaggggggc gtgacagtta
    13141 ttgcaggggt tcgtgacagt tagtacggga gtgacgggca ctggctggca atgtctagca
    13201 acggcaggca tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa
    13261 acacagtatt gcaaggacgc ggaacatgcc tcatgtggcg gccctcgcga gaattaattc
    13321 agataaaaaa aatccttagc tttcgctaag gatgatttct agcgatgacc ctgctgattg
    13381 gttcgctgac cgtagactgg ttggcttggt ttcatcagcc atccgcttgc cctcatctgt
    13441 tacgccggcg gtagccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg
    13501 cgaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct
    13561 gcccggctga cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc
    13621 tgtatatcgt gcgacatcgc gcgcgcgcgc gcggatgcta gcggcgcgcc aaaaaaaagc
    13681 ccgctcatta
//
