LOCUS       sequence-467497-       13564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate 
            inducible promoter, without accessory genes (inactive), in 
            Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13564)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 13564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80EN10-Pct3-(–)-Eldh1(cHis6)-noAccs(ElA2)
FEATURES             Location/Qualifiers
     source          1..13564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(1497..2108)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2512..2539
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     regulatory      4309..4318
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5611..5628
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      7188..7215
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      7347..7374
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(7806..8657)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(8644..9483)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(9994..10965)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            12204..12291
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      12633..13027
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(13190..13233)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            13329..13438
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctag
     1501 cgcttgaatt tcgcgtaccg ctctcgcgca atttcgagtg tcgagttcct gacacgctca
     1561 aagcgttctt tgtccttctg ccataggcta cgaacagcaa gcccacgcac cgaattgaat
     1621 atcaaccaaa ggatatcttc tgcatcatca cgcgaaagac cacggctcac cagaacacca
     1681 agccacatat cctcgacgac aaaccgattc ctctctaccg tgcgctgaat accctcgcgt
     1741 aacgctgggt cccggtcggc agccacaatc aaatcaaggc tgatagagaa gtcatcgtcg
     1801 aggaaaaatt cggcggcgtc gtccagcatt tgctggatga cgtcatcctc tggcttcaat
     1861 ttcgctaatc gagcccgact gcgttcggtg atctgttcgt aaagccattc aaaagtggca
     1921 agcagaagct caagctttgt cgggaaatga tggctctgcg ctcctctgga gacaccagca
     1981 gcaccgggca catctgcgat ccggaatccc gcgtaacctt tttcccgtaa aacccccagg
     2041 gccgctgcaa tcaacttgcc ctgggtctcc attgcgcgct ctgcctgtgt tcttctcttt
     2101 ggactcatga tcaccagcga cctgctttcg ttcgtccggg catcacccgg gttactatct
     2161 agtatcaata actagattat tggttgaaac gtactatgtc aagcagttct aaagattgac
     2221 tccaaacggt cgccttgcta tgctcgtttc gtttcctccg cacgcgcgcg aaaggctcct
     2281 tcacggagcc aatgtacgca aaaaaccccg cttcagcggg gttttttcgc caaaaaaaac
     2341 cccgccctgt caggggcggg gttttttttt ttagggccct actgactgtg agcccgctcg
     2401 atcaacggat cggtaaaata cgggcgaaaa agagaatccc gagctttttt gttgccgcat
     2461 agcgtatagt aagcatgtgc cctcgaggtg agggcgagtg ttgcggccag taacaaacag
     2521 acaatctggt ctgtttgtat tatgcagcac acagtgttac ctcctcgggg acgaggagag
     2581 gaaagaggat ttaaaatggg gaagacgacc gtgacaaggc gcgcgttcgc tcagcttgcc
     2641 gccgcgacag gcgcaatagc cgctatgggt gttggaactc gtcctgcggt ggcgcttacc
     2701 gatggcaatt cggggacggc gggcgagcgg gggatcaaga agatacgctc gtgctgccgc
     2761 ggctgcggaa aggtcgaatg cggtgtgtgg gtgtacatcc aagatggcaa agtggttcga
     2821 accgaaggtg acgaaacctg cttcaacact atgggcaatc attgcagcaa ggggcaagcg
     2881 tccattcagg ctgcatatca tcctgatcgt atcaagttcc caatgaagcg cacgaacccg
     2941 aagggcagcg aagatccagg ttgggtgagg atcagctggg acgaggccta ccggaccatt
     3001 gcggacaata ttatgcagtt gcgtgagaag tacggtcctg aaagcttgtt cacgtggtgc
     3061 ggtacgggcc gacagtggtg catgcagtcc gatgctggca tggcgcttga gctgttcggt
     3121 acgccgaaca tcatcgcggc gtaccaagtg tgcaagggtc cgcgccattt ttcgtcgcgt
     3181 ctcgacaacg ttcaggcgtg gtcttggagt gaggtgataa accattcgac gaaatacgtc
     3241 caatgggcta ccgatccctc cgtttcgaat tacgacgatt cgtctcgttt ggtaattgat
     3301 gtggcgcgcg aagctgaggc ttttatcgtg gttgaccctc gcctgtcgaa tcttggccgc
     3361 actgcgaagt attggttaaa cttgcgacct ggcaccgata atgccatggc gctaggatgg
     3421 tgccatatca tattgaagca cgatctggtg gattggcagt tcgtgaaacg ctggtccaat
     3481 gcctcgttca tcgtcgtgcc cgatatggac ccttcgggct acaccgaggc ggttcaaaac
     3541 acgaaaagcc cctacgaata tcgtacgcgt ttgctgaccg aagccgacat agatccttct
     3601 atggttgact gggagattga aggcgagggg aatccaaaac gttatcttgt gtacgatcag
     3661 atcaatcggc gctggacata ttggcaagcc gatcccgaag acgcgcattg ggaaggcgag
     3721 acctggacaa aacaaacctc tggattcacg caagacgttt cgcgtcttcg cgacgacgaa
     3781 tcgaaggtgg ctggctggat cgcggatctg tcggaattcg atccgcgtat cgatccggcg
     3841 ctcaccgggg aattcgaagt aaggcttaag gacggaagta cgcatatcgg ccgtcctgca
     3901 tgggatttgt gggctgagta cctgcaacaa ttcactcccg aacgagtgtc ggagatcacg
     3961 ggcgtagatg cccagcttat cgaagacgcc gtggttgaat gggcaacgcg cgatgacccg
     4021 cgcataccca acggcggtat aaattacggt ctgggcgtgg agcatgcggg caactcaacg
     4081 cagaattgca gggccattat ggcggcttgc gcaatggtag gcgccatcga cacgccgggc
     4141 ggccagcgcg gtgcgacgaa cggatggacc gaacagtcgg gtccgtgcgc catgcttccg
     4201 tcgatggcgg cattcgcgtt catgcccacg cccgacttgt cgctcaagat ggccggaaat
     4261 gagaagatgc cgcttctata ctggtacggt gtgtggtgcg atgccaatgc cgccatggaa
     4321 tgcgcgcaca aggagccgga tgcgccctat gagattcatg gtggcatgat cggctcgggc
     4381 gaccatatga acatgggcaa cgcaacgtac aattgggaag cgctcaacat gctcgacttt
     4441 ctgttcgagg cgaatctgtg gcattcccct acttcaggtg cggccgacat cctccttccg
     4501 gtctgccatt ggaccgagat caacgcgtat cgcattgccc aaggggcatc cggtgggttt
     4561 ggcctatgcg tgaaagctgt tgatcctcct ggcgaatgca aaagcgatcc gttgtacttc
     4621 atggagctgt cgaagtattt cggcgtccca gcgtttgacg gcgacgatcc gtggctcgag
     4681 aacaaacctg atgccgatct cgaaatcgaa aacctcacga ttcagtgctg tgtccaagga
     4741 tgcgcgccat acaacaattg gaacgagttg gaggctgcat tccaagagca cggttggtgg
     4801 aacatgaaac gcgagatccc ggaagactgg ggcacgtacc gtcgatacga ggtggggcag
     4861 gcgtatcggt tggctcccca tcagcaaccg gctcagctga acatcaataa accggggttc
     4921 cccacaccta cgatgaaaca tgagttttgg tgcacttcca tcgaatcgtt cttcccggag
     4981 ggggcggatg gccctgagct tgcacccggg ttcacttccg aagcgctgcc ttattatgca
     5041 gagcccgcac acggccctgt ggtcgatgcg gaaacgtaca aggagtatcc cattacctgc
     5101 atcactggac gacgcatacc ggtgtacttc cactccgagc accgacagct gccctggtgt
     5161 cgcgagcttt ggccggtgcc tcgcatggag atcaatcccg atacggctgc tgaacttgga
     5221 ctcgaacaag gagattgggc ctggatcgag agcccttggg gcaaggtgcg acagacggtc
     5281 gatctctatt acggcattaa gccgaacatg atcaacgccg agcatcaatg gtggtatccg
     5341 gagttggctc aagcggacaa aggatatgag ttgtcatgca tcaattgcat taccgatcgg
     5401 aagactcagg ataaatacaa tggatcgtcg aatgtgcgca cctatccagt gaaggtgtac
     5461 aaagccacgc ctgagaattc cccctttggg aatcctatcc cttgcggaaa cgatgggact
     5521 gagatcattc atgactcttc tgatcctcgc ctcaagctat gggaaatcgg cgctgctggc
     5581 atccatccgg atcacttcga gggcagcagc catcaccatc accatcacta ggagaggggg
     5641 aattatgacg cagtacgcta tcgtcaccga tctcgatcgt tgcgtaggtt gtcttgcctg
     5701 ttcggttgcc tgcaaggtag tcaacggcgt tccgacgggc tcgttttgga acaagacatt
     5761 gcgcattggc ccgaatcctc gagaaggcgg cagtgggcaa tggcccgatg tggagctgta
     5821 ttttctcaat gtgcagtgcc agcactgcga gaaccccgaa tgcgtgaagg tgtgcccgac
     5881 gggcgcctcg cacaagaccg aggacggcac cgtgcagatc gacaagtcga agtgcatcgg
     5941 ctgccagttc tgcgcgatgt cgtgcccgta cggggtgcgc tacctcaacg aggaggagcg
     6001 cgtcgtggag aagtgcacct tgtgcgagca gaagatcgct cagggcgaac ttcctcagtg
     6061 cgtgagtcaa tgctgcggta tggcacgctg gttcggcgat gcggacgaag gtatcgaagg
     6121 tttcgtcggg cctcgcggcg agatactggg cgagtatata tcgccattct cggatgatca
     6181 ggtgtatagc ctgccggatg tgggcaataa gccgtcgctc aggtacatct tgcgcgatat
     6241 gaaatggcaa ggaggcggtg aatgatggaa ctgcaatggc ctttgatttt gttcaccacg
     6301 ctggttgcgt ggagcgcggg gctgttcggg acgcaggcgc tgatggcggt gttcggcgtc
     6361 gggaagaagg cgcaggtgcc ggcttgggtt tgctcggccg tcttgctggc ggccggcggg
     6421 atcgcggtgt tcttccatct cgagcactgg gagcggatct tcaacgggtt cgggcatttg
     6481 acgagcggga tcacgcagga attgatcgcg atcgtggtct tggccgtggt ggctatcgtc
     6541 tacttggtgc tcatgcgcaa gtccgacgac ggcgccagcg tgccgaagtg gctggcatgg
     6601 gtctccgtcg ccttgtcggt cgtgctcgtg gccgtcatgg cgcacagcta cacgatggcc
     6661 gcccgccccg cctgggactc ggccctctgg atcctctacg tcctcggcaa cgcctgcgtg
     6721 ttgggccccg cgacgttcgc cctcctctcc gccttggctg cagggggacc ccgggaccag
     6781 cccgccgagc gcgcagccga cgccggggcc cccgcgggtc tcgcggccct cgccggcgcc
     6841 gccctcaacg ccctcgcggc cctcgccttc gccgccttcc tccagctctc cgccggctcc
     6901 ttcgccgacg tcggcctcta cttcgacccg acgcacccca cgaaggccat ggccgacgcc
     6961 gccgcgacgg tcgcctccca ggccccgctg ctgtggctgg gcgccgtggc cgtgggcgcg
     7021 atcgtgcccc tggccgccgc cttcctaggc aggcgcacgg ggaactggaa gctctgggtc
     7081 ccggtcgcga tagccgccgc cctggtcggc gccgtctgca tgcgcgtcgt cttctacaac
     7141 ctggggctca gcgtgttcat gttctactag actagtcgac tcacactggc tcaccttcgg
     7201 gtgggccttt ctgcgtttat atactagaga gagaatataa aaagccagat tattaatccg
     7261 gcttttttat tatttaattc ctgttataaa aaaaggatca attttgaact ctctcccaaa
     7321 gttgatccct taacgatccc tgcaggagaa ggccatcctg acggatggcc tttttgaagc
     7381 tttctagaat cgggggcccc cggggggatt ctagaacaat ggaagtcctc gtctcgccct
     7441 cgaattagcc cgcttaatga gcgggctttt tttgaattaa ttctcgcgag ctggcacgac
     7501 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag ttagcgcgaa
     7561 ttgcaagctg gccgacgcgc tgggctacgt cttgctggcg ttcgggagca gaagagcata
     7621 catctggaag caaagccagg aaagcggcct atggagctgt gcggcagcgc tcagtaggca
     7681 atttttcaaa atattgttaa gccttttctg agcatggtat ttttcatggt attaccaatt
     7741 agcaggaaaa taagccattg aatataaaag ataaaaatgt cttgtttaca atagagtggg
     7801 gggggtcagc ctgccgcctt gggccgggtg atgtcgtact tgcccgccgc gaactcggtt
     7861 accgtccagc ccagcgcgac cagctccggc aacgcctcgc gcacccgctg gcggcgcttg
     7921 cgcatggtcg aaccactggc ctctgacggc cagacatagc cgcacaaggt atctatggaa
     7981 gccttgccgg ttttgccggg gtcgatccag ccacacagcc gctggtgcag caggcgggcg
     8041 gtttcgctgt ccagcgcccg cacctcgtcc atgctgatgc gcacatgctg gccgccaccc
     8101 atgacggcct gcgcgatcaa ggggttcagg gccacgtaca ggcgcccgtc cgcctcgtcg
     8161 ctggcgtact ccgacagcag ccgaaacccc tgccgcttgc ggccattctg ggcgatgatg
     8221 gataccttcc aaaggcgctc gatgcagtcc tgtatgtgct tgagcgcccc accactatcg
     8281 acctctgccc cgatttcctt tgccagcgcc cgatagctac ctttgaccac catggcatca
     8341 gcggtgacgg cctcccactt gggttccagg aacagccgga gctgccgtcc gccttcggtc
     8401 ttgggttccg ggccaagcac taggccatta ggcccagcca tggccaccag cccttgcagg
     8461 atgcgcagat catcagcgcc cagcggctcc gggccgctga actcgatccg cttgccgtcg
     8521 ccgtagtcat acgtcacgtc cagcttgctg cgcttgcgct cgccccgctt gagggcacgg
     8581 aacaggccgg gggccagaca gtgcgccggg tcgtgtcgga cgtggctgag gctgtgcttg
     8641 ttcttaggct tcaccacggg gcaccccctt gctcttgcgc tgcctctcca gcacggcggg
     8701 cttgagcacc ccgccgtcat gccgcctgaa ccaccgatca gcgaacggtg cgccatagtt
     8761 ggccttgctc acaccgaagc ggacgaagaa ccggcgctgg tcgtcgtcca caccccattc
     8821 ctcggcctcg gcgctggtca tgctcgacag gtaggactgc cagcggatgt tatcgaccag
     8881 taccgagctg ccccggctgg cctgctgctg gtcgcctgcg cccatcatgg ccgcgccctt
     8941 gctggcatgg tgcaggaaca cgatagagca cccggtatcg gcggcgatgg cctccatgcg
     9001 accgatgacc tgggccatgg ggccgctggc gttttcttcc tcgatgtgga accggcgcag
     9061 cgtgtccagc accatcaggc ggcggccctc ggcggcgcgc ttgaggccgt cgaaccactc
     9121 cggggccatg atgttgggca ggctgccgat cagcggctgg atcagcaggc cgtcagccac
     9181 ggcttgccgt tcctcggcgc tgaggtgcgc cccaagggcg tgcaggcggt gatgaatggc
     9241 ggtgggcggg tcttcggcgg gcaggtagat caccgggccg gtgggcagtt cgcccacctc
     9301 cagcagatcc ggcccgcctg caatctgtgc ggccagttgc agggccagca tggatttacc
     9361 ggcaccaccg ggcgacacca gcgccccgac cgtaccggcc accatgttgg gcaaaacgta
     9421 gtccagcggt ggcggcgctg ctgcgaacgc ctccagaata ttgataggct tatgggtagc
     9481 cattgattgc ctcctttgca ggcagttggt ggttaggcgc tggcggggtc actacccccg
     9541 ccctgcgccg ctctgagttc ttccaggcac tcgcgcagcg cctcgtattc gtcgtcggtc
     9601 agccagaact tgcgctgacg catccctttg gccttcatgc gctcggcata tcgcgcttgg
     9661 cgtacagcgt cagggctggc cagcaggtcg ccggtctgct tgtccttttg gtctttcata
     9721 tcagtcaccg agaaacttgc cggggccgaa aggcttgtct tcgcggaaca aggacaaggt
     9781 gcagccgtca aggttaaggc tggccatatc agcgactgaa aagcggccag cctcggcctt
     9841 gtttgacgta taaccaaagc caccgggcaa ccaatagccc ttgtcacttt tgatcaggta
     9901 gaccgaccct gaagcgcttt tttcgtattc cataaaaccc ccttctgtgc gtgagtactc
     9961 atagtataac aggcgtgagt accaacgcaa gcactacatg ctgaaatctg gcccgcccct
    10021 gtccatgcct cgctggcggg gtgccggtgc ccgtgccagc tcggcccgcg caagctggac
    10081 gctgggcaga cccatgacct tgctgacggt gcgctcgatg taatccgctt cgtggccggg
    10141 cttgcgctct gccagcgctg ggctggcctc ggccatggcc ttgccgattt cctcggcact
    10201 gcggccccgg ctggccagct tctgcgcggc gataaagtcg cacttgctga ggtcatcacc
    10261 gaagcgcttg accagcccgg ccatctcgct gcggtactcg tccagcgccg tgcgccggtg
    10321 gcggctaagc tgccgctcgg acagttcgag gctggccagc ctgcgggcct tctcctgctg
    10381 ccgctgggcc tgctcgatct gctggccagc ctgctgcacc agcgccgggc cagcggtggc
    10441 ggtcttgccc ttggattcac gcagcagcac ccacggctga taaccggcgc gggtggtgtg
    10501 cttgtccttg cggttggtga agcccgccaa gcggccatag tggcggctgt cggcgctggc
    10561 cgggtcggcg tcgtactcgc tggccagcgt ccgggcaatc tgcccccgaa gttcaccgcc
    10621 tgcggcgtcg gccaccttga cccatgcctg atagttcttc gggctggttt ccactaccag
    10681 ggcaggctcc cggccctcgg ctttcatgtc atccaggtca aactcgctga ggtcgtccac
    10741 cagcaccaga ccatgccgct cctgctcggc gggcctgata tacacgtcat tgccctgggc
    10801 attcatccgc ttgagccatg gcgtgttctg gagcacttcg gcggctgacc attcccggtt
    10861 catcatctgg ccggtggtgg cgtccctgac gccgatatcg aagcgctcac agcccatggc
    10921 cttgagctgt cggcctatgg cctgcaaagt cctgtcgttc ttcatcgggc caccaagcgc
    10981 agccagatcg agccgtcctc ggttgtcagt ggcgtcaggt cgagcaagag caacgatgcg
    11041 atcagcagca ccaccgtagg catcatggaa gccagcatca cggttagcca tagcttccag
    11101 tgccaccccc gcgacgcgct ccgggcgctc tgcgcggcgc tgctcacctc ggcggctacc
    11161 tcccgcaact ctttggccag ctccacccat gccgcccctg tctggcgctg ggctttcagc
    11221 cactccgccg cctgcgcctc gctggcctgc tgggtctggc tcatgacctg ccgggcttcg
    11281 tcggccagtg tcgccatgct ctgggccagc ggttcgatct gctccgctaa ctcgttgatg
    11341 cctctggatt tcttcactct gtcgattgcg ttcatggtct attgcctccc ggtattcctg
    11401 taagtcgatg atctgggcgt tggcggtgtc gatgttcagg gccacgtctg cccggtcggt
    11461 gcggatgccc cggccttcca tctccaccac gttcggcccc aggtgaacac cgggcaggcg
    11521 ctcgatgccc tgcgcctcaa gtgttctgtg gtcaatgcgg gcgtcgtggc ctgcccgctc
    11581 taatgcccgg ttggcatggt cggcccatgc ctcgcgggtc tgctcaagcc atgccttggg
    11641 cttgagcgct tcggtcttct gtgccccgcc cttctccggg gtcttgccgt tgtaccgctt
    11701 gaaccactga gcggcgggcc gctcgatgcc gtcattgatc cgctcggaga tcatcaggtg
    11761 gcagtgcggg ttctcgccgc caccggcatg gatggccagc gtatacggca ggcgctcggc
    11821 accggtcagg tgctgggcga actcggacgc cagcgccttc tgctggtcga gggtcagctc
    11881 gaccggcagg gcaaattcga cctccttgaa cagccgccca ttggcgcgtt catacaggtc
    11941 ggcagcatcc cagtagtcgg cgggccgctc gacgaactcc ggcatgtgcc cggattcggc
    12001 gtgcaagact tcatccatgt cgcgggcata cttgccttcg cgctggatga agtcggcctt
    12061 ggccctggcc gattggccgc ccgacctgct gccggttttc gccgtaaggt gataaatcgc
    12121 catgctgcct cgctgttgct tttgcttttc ggctccatgc aatggccctc ggagagcgca
    12181 ccgcccgaag ggtggccgtt aggccagttt ctcgaagaga aaccggtaag tgcgccctcc
    12241 cctacaaagt agggtcggga ttgccgccgc tgtgcctcca tgatagccta cgagacagca
    12301 cattaacaat ggggtgtcaa gatggttaag gggagcaaca aggcggcgga tcggctggcc
    12361 aagctcgaag aacaacgagc gcgaatcaat gccgaaattc agcgggtgcg ggcaagggaa
    12421 cagcagcaag agcgcaagaa cgaaacaagg cgcaaggtgc tggtgggggc catgattttg
    12481 gccaaggtga acagcagcga gtggccggag gatcggctca tggcggcaat ggatgcgtac
    12541 cttgaacgcg accacgaccg cgccttgttc ggtctgccgc cacgccagaa ggatgagccg
    12601 ggctgaatga tcgaccgaga caggccctgc ggggctgcac acgcgccccc acccttcggg
    12661 tagggggaaa ggccgctaaa gcggctaaaa gcgctccagc gtatttctgc ggggtttggt
    12721 gtggggttta gcgggctttg cccgcctttc cccctgccgc gcagcggtgg ggcggtgtgt
    12781 agcctagcgc agcgaataga ccagctatcc ggcctctggc cgggcatatt gggcaagggc
    12841 agcagcgccc cacaagggcg ctgataaccg cgcctagtgg attattctta gataatcatg
    12901 gatggatttt tccaacaccc cgccagcccc cgcccctgct gggtttgcag gtttgggggc
    12961 gtgacagtta ttgcaggggt tcgtgacagt tattgcaggg gggcgtgaca gttattgcag
    13021 gggttcgtga cagttagtac gggagtgacg ggcactggct ggcaatgtct agcaacggca
    13081 ggcatttcgg ctgagggtaa aagaactttc cgctaagcga tagactgtat gtaaacacag
    13141 tattgcaagg acgcggaaca tgcctcatgt ggcggccctc gcgagaatta attcagataa
    13201 aaaaaatcct tagctttcgc taaggatgat ttctagcgat gaccctgctg attggttcgc
    13261 tgaccgtaga ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
    13321 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
    13381 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
    13441 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
    13501 tcgtgcgaca tcgcgcgcgc gcgcgcggat gctagcggcg cgccaaaaaa aagcccgctc
    13561 atta
//
