LOCUS       sequence-467548-       11227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor DOT1L with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11227)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 11227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-DOT1L-Brd 425
FEATURES             Location/Qualifiers
     source          1..11227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(93..112)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(94..112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(122..139)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(122..138)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(131..153)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      280..735
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(367..386)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     577..598
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        761..865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(866..934,935..1726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1084..1103)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1897..2485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2386..2405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2639..2656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2773..2794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2809..2814,2815..2832,2833..2839)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2847..2863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2852..2874
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2871..2887
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2871..2887
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2906..2926
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2908..2926
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2954..3180
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3181..3361
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3408..3533
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4026..4259
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4444..4488
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4755..4872
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4906..5312
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    10177..10580
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             10699..10932
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11004..11125
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11041..11060)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11095..11114
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      join(11165..11227,1..73)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
       61 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
      121 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      181 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      241 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
      301 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      361 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      421 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      481 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      541 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      601 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
      661 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
      721 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      781 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      841 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      901 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      961 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1021 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1081 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1141 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1201 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1261 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1321 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1381 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1441 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1501 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1561 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1621 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1681 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1741 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1801 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1861 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1921 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1981 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2041 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2101 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2161 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2221 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2281 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2341 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2401 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2461 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2521 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2581 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2641 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2701 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     2761 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     2821 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     2881 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     2941 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     3001 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     3061 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     3121 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     3181 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     3241 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     3301 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3361 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     3421 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     3481 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     3541 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     3601 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     3661 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     3721 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     3781 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     3841 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     3901 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     3961 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     4021 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     4081 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     4141 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     4201 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     4261 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     4321 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     4381 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     4441 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     4501 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     4561 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     4621 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     4681 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     4741 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     4801 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     4861 attcaaaatt ttatcgatca cgagactagc ctcgagctgg ctgcagtaac gccattttgc
     4921 aaggcatgga aaaataccaa accaagaata gagaagttca gatcaagggc gggtacatga
     4981 aaatagctaa cgttgggcca aacaggatat ctgcggtgag cagtttcggc cccggcccgg
     5041 ggccaagaac agatggtcac cgcagtttcg gccccggccc gaggccaaga acagatggtc
     5101 cccagatatg gcccaaccct cagcagtttc ttaagaccca tcagatgttt ccaggctccc
     5161 ccaaggacct gaaatgaccc tgcgccttat ttgaattaac caatcagcct gcttctcgct
     5221 tctgttcgcg cgcttctgct tcccgagctc tataaaagag ctcacaaccc ctcactcggc
     5281 gcgccagtcc tccgacagac tgagtcggcc ggtcgaatca agcttatcga taccgtcgac
     5341 ggatccgcta gcccgcggtt cgaaatggga gaaaagttag aactccgtct caaatcccct
     5401 gttggagcag aacctgcggt atatccttgg cccttgcccg tgtatgacaa gcaccatgac
     5461 gcggcacacg agataattga aacaattagg tgggtgtgcg aggagatacc tgacctgaaa
     5521 ctggccatgg aaaactatgt gctcatcgat tacgatacaa agtcttttga atcaatgcaa
     5581 cggctttgtg ataaatataa cagggcaatt gactcaatac atcaactctg gaaaggaacg
     5641 actcaaccta tgaaacttaa tacaagaccc tcaaccgggc tgcttcggca catcttacaa
     5701 caagtgtata atcattctgt cactgatcca gaaaagctga ataattacga acccttttct
     5761 ccagaagtct atggagaaac atcttttgat ctcgtcgcac aaatgatcga cgaaattaaa
     5821 atgactgatg atgatctttt cgtagatctg ggctccggcg taggacaagt agttctgcaa
     5881 gtggcagcgg caactaattg taagcaccat tacggggtgg aaaaggccga tattcccgct
     5941 aaatacgcag aaacaatgga tcgagaattt agaaaatgga tgaagtggta cggtaagaaa
     6001 cacgcggagt atactcttga aagaggggac ttcttaagcg aggaatggag agaacgcatt
     6061 gctaatacct ctgtgatttt cgtcaacaac ttcgctttcg ggccagaagt agaccatcaa
     6121 cttaaagaaa gattcgctaa tatgaaagag ggcggtagga ttgtcagctc caagcctttc
     6181 gccccactca attttcggat caatagccgg aatctttctg atatagggac aattatgagg
     6241 gttgtcgaac tttcaccatt aaaagggtct gtaagttgga caggcaaacc tgtatcttat
     6301 tatctccata caattgatag aacaatcctc gagaattact tctccagcct caagaatcct
     6361 aagttacgcg aagagcaaga agcggctaga aggcgacaac aaagggaaag taaatccaat
     6421 gcagctacac caacaaaagg acccgaagga aaagttgcag gtcctgcaga tgcaccaatg
     6481 gattcaggcg ccgaagagga gaaagccggg gccgcgacag tcaagaagcc tagcccaagt
     6541 aaggctagaa agaagaaatt gaataagaag ggtcgaaaga tggccggacg taaaagaggc
     6601 cggccaaaga aaatgaatac agctaatcca gaacgcaaac ctaagaagaa tcagaccgct
     6661 ttagacgctt tgcatgccca aacggtctca caaacagcag cgagctcccc tcaagacgca
     6721 tatcgcagcc cccattcccc cttttatcaa ctgccccctt ctgtccaacg acatagccca
     6781 aatcctcttc ttgttgctcc aacaccccct gctcttcaaa agctgctgga atcatttaaa
     6841 attcaatatc tccaatttct cgcctatacc aaaaccccac aatacaaagc ttcactccaa
     6901 gaacttcttg gtcaagagaa agagaagaat gcacaactgt tgggagcagc ccaacaactg
     6961 ttatcccatt gtcaagctca gaaagaagaa ataagaagac ttttccaaca gaagctcgac
     7021 gaactcgggg tcaaagccct tacatataat gatttgatcc aggctcagaa agaaataagc
     7081 gctcacaatc aacaattgag ggaacaatcc gaacaattag aacaagataa tagagcactg
     7141 aggggtcaat ccttgcaact tttgaaagcc aggtgtgaag aactccaact cgattgggca
     7201 acactcagtc ttgagaaact gctcaaggag aaacaagctt taaaatcaca aataagcgaa
     7261 aagcaaagac attgtttgga actccagata tccatagtcg aacttgaaaa gtctcaacgc
     7321 caacaagaac tgcttcaact gaaaagctgc gttccacccg atgatgcgct cagcctgcat
     7381 cttcgaggaa aaggagcttt gggacgggaa cttgaacccg atgcatcacg ccttcattta
     7441 gaattagatt gtacaaaatt tagcttgccc catctgtcct caatgtcccc agaactgtct
     7501 atgaatggac aagcggccgg gtacgaactg tgtggcgtac tttccaggcc ctcttcaaaa
     7561 caaaatacac cacaatattt ggcgtcccct ctcgatcaag aagttgttcc atgcacacca
     7621 tcacatgtgg ggcgacctag acttgagaaa ttatccgggc tggcggctcc tgattataca
     7681 cgactctctc ccgcgaaaat cgttttgcga cgtcatctta gtcaagatca taccgtccca
     7741 ggacgaccag ccgctagcga acttcacagc cgtgccgaac atactaaaga aaatgggctg
     7801 ccttatcaat caccgtcagt ccccggtagt atgaaacttt ccccacaaga tccccgccct
     7861 ctttctcccg gagcactcca actcgccggg gaaaagagtt ctgagaaagg gctcagggaa
     7921 agagcatatg gttcttccgg agaactgatt acatccttgc ctattagtat tcctttgtca
     7981 acggtccaac cgaataaact tcccgtttct atcccactcg ctagtgttgt actcccaagt
     8041 agggcagaac gtgcccgaag tactccatct ccagtccttc aaccaaggga tccgtccagc
     8101 actctggaga aacaaatcgg cgccaacgca catggcgccg gttctaggtc attagcgttg
     8161 gctccagctg ggtttagtta tgccggaagc gttgcaatat caggagctct tgcaggatcc
     8221 cccgcatcac tgaccccagg cgctgaaccc gcaacattag acgaaagcag ctcttccgga
     8281 tctctcttcg cgactgttgg ctcacgttca agcacccctc aacatccact cctcttagcc
     8341 caaccacgca atagtctgcc cgctagccca gcgcatcaac tgagctcaag ccctagactg
     8401 ggcggcgctg cgcaagggcc cctgccagaa gcgtctaaag gtgatttgcc gagcgacagt
     8461 ggattttccg acccagaaag cgaggctaaa cgccgtatag tctttacaat tacaaccggg
     8521 gccggttctg ctaaacaatc ccctagttca aaacatagtc ctcttacagc atccgctagg
     8581 ggagattgcg tcccaagtca tggtcaagat tctcgtcgac ggggtagaag gaaaagagcc
     8641 agcgccggca ctccatctct gtccgccggg gtttctccta aaagacgcgc tctccctagc
     8701 gttgccgggc tctttactca accatcagga tccccattga atctgaatag catggtgtcc
     8761 aatattaatc aaccactcga aataactgca attagcagcc cagaaacttc actcaaatct
     8821 tcacccgttc cttatcaaga tcatgatcaa ccacctgttt tgaagaaaga acgaccgctc
     8881 tctcaaacaa acggagccca ttattcaccc ctgacaagtg atgaagaacc ggggtccgaa
     8941 gatgaacctt cctctgcccg catagaacgc aagatcgcca ccatttcact tgagagtaag
     9001 tccccaccta agactctgga gaacggtggc gggctggctg gtcgtaaacc agcccctgca
     9061 ggagaacccg tgaactcttc caaatggaaa tctacatttt caccgattag cgatataggg
     9121 ttggctaaat ctgctgattc tcctcttcaa gcttcttctg ctctgtctca aaatagtctc
     9181 tttaccttta ggcctgcact cgaagaacca agcgcagacg cgaaactcgc agcccatcct
     9241 cggaagggat tcccaggaag cctttccggc gcagatggtt tgtctcccgg gacgaatccg
     9301 gcaaatggat gtacttttgg cggtggactc gctgctgatc tcagcctgca tagtttctcc
     9361 gacggcgcct cattgcctca taaaggacct gaagctgctg gactctcaag ccccctttct
     9421 tttccgtccc aaagaggtaa agaaggtagc gatgctaatc cctttctgag caaaagacaa
     9481 cttgatggtc tcgccggtct taaaggagaa gggagtcgtg gtaaagaagc tggagaaggc
     9541 gggttgccac tctgtggccc aactgataaa actccccttc tttccggaaa agcggctaaa
     9601 gctcgtgatc gtgaagtgga tctgaagaac gggcataatc tgtttatatc cgctgctgct
     9661 gtgcccccag gtagtttgct gtccggtcca gggttggcgc cagctgcttc atctgccggt
     9721 ggtgccgcct catctgcaca aacccatcgc agctttcttg gaccatttcc ccctgggcca
     9781 caatttgcac tgggtcctat gagccttcaa gcaaatttgg ggtctgtcgc tgggagcagt
     9841 gtgctccaat cccttttcag tagcgtccct gctgctgctg ggttggttca tgtttccagt
     9901 gccgcaacta ggctcacaaa tagccatgct atgggatctt tcagcggcgt cgccggtgga
     9961 actgtaggtg gaaattgaga attcgagcat cttaccgcca tttattccca tatttgttct
    10021 gtttttcttg atttgggtat acatttaaat gttaataaaa caaaatggtg gggcaatcat
    10081 ttacattttt agggatatgt aattactagt tcaggtgtat tgccacaaga caaacatgtt
    10141 aagaaacttt cccgttattt acgctctgtt cctgttaatc aacctctgga ttacaaaatt
    10201 tgtgaaagat tgactgatat tcttaactat gttgctcctt ttacgctgtg tggatatgct
    10261 gctttaatgc ctctgtatca tgctattgct tcccgtacgg ctttcgtttt ctcctccttg
    10321 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtccg tcaacgtggc
    10381 gtggtgtgct ctgtgtttgc tgacgcaacc cccactggct ggggcattgc caccacctgt
    10441 caactccttt ctgggacttt cgctttcccc ctcccgatcg ccacggcaga actcatcgcc
    10501 gcctgccttg cccgctgctg gacaggggct aggttgctgg gcactgataa ttccgtggtg
    10561 ttgtcgggga agctgacgtc ctttgcaatt cgatatcaag ctgtaccttc tagatgtaca
    10621 tctcgggtag cgcttctaga ccaatgactt acaaggcagc tgtagatctt agccactttt
    10681 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
    10741 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
    10801 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
    10861 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
    10921 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
    10981 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
    11041 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    11101 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
    11161 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    11221 ttttttt
//
