LOCUS       sequence-467552-        3762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Marker_SpcR-StrR-aadA_PartType2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK004 [Marker_SpcR-StrR-aadA_PartType2]
FEATURES             Location/Qualifiers
     source          1..3762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1497..2288
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        2740..2831
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2832..2900,2901..3692)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      3696..3735
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctcaatc cggggatccg gtgattgatt gagcaagctt tatgcttgta
      661 aaccgttttg tgaaaaaatt tttaaaataa aaaaggggac ctctagggtc cccaattaat
      721 tagtaatata atctattaaa ggtcattcaa aaggtcatcc accggatcaa ttcccctgct
      781 cgcgcaggct gggtgccaag ctctcgggta acatcaaggc ccgatccttg gagcccttgc
      841 cctcccgtac gatgatcgtg ccgtgatcga aatccagatc cttgacccgc agttgcaaac
      901 cctcactgat ccgcatgccc gttccataca gaagctgggc gaacaaacga tgctcgcctt
      961 ccagaaaacc gaggatgcga accacttcat ccggggtcag caccaccggc aagcgccgcg
     1021 acggccgagg tcttccgatc tcctgaagcc agggcagatc cgtgcacagc accttgccgt
     1081 agaagaacag caaggccgcc aatgcctgac gatgcgtgga gcccgaaacc ttgcgctcgt
     1141 tcgccagcca ggacagaaat gcctcgactt cgctgctgcc caaggttgcc gggtgacgca
     1201 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     1261 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     1321 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     1381 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     1441 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga
     1501 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     1561 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     1621 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     1681 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     1741 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     1801 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     1861 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     1921 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     1981 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     2041 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     2101 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     2161 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     2221 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     2281 gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     2341 gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt
     2401 tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat catgaaaggc
     2461 tggctttttc ttgttatcgc aatagttggc gaagtaatcg caacatccgc attaaaatct
     2521 agcgagggct ttactaagct gatccggtgg atgacctttt gaatgacctt taatagatta
     2581 tattactaat taattgggga ccctagaggt cccctttttt attttaaaaa ttttttcaca
     2641 aaacggttta caagcataaa gcttgctcaa tcaatcaccg gatccccctt gagaccagag
     2701 tagcccgcct aatgagcggg ctttttttta attcccctat ttgtttattt ttctaaatac
     2761 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     2821 aaaggaagag tatgagcatt cagcattttc gtgtggcgct gattccgttt tttgcggcgt
     2881 tttgcctgcc ggtgtttgcg catccggaaa ccctggtgaa agtgaaagat gcggaagatc
     2941 aactgggtgc gcgcgtgggc tatattgaac tggatctgaa cagcggcaaa attctggaat
     3001 cttttcgtcc ggaagaacgt tttccgatga tgagcacctt taaagtgctg ctgtgcggtg
     3061 cggttctgag ccgtgtggat gcgggccagg aacaactggg ccgtcgtatt cattatagcc
     3121 agaacgatct ggtggaatat agcccggtga ccgaaaaaca tctgaccgat ggcatgaccg
     3181 tgcgtgaact gtgcagcgcg gcgattacca tgagcgataa caccgcggcg aacctgctgc
     3241 tgacgaccat tggcggtccg aaagaactga ccgcgtttct gcataacatg ggcgatcatg
     3301 tgacccgtct ggatcgttgg gaaccggaac tgaacgaagc gattccgaac gatgaacgtg
     3361 ataccaccat gccggcagca atggcgacca ccctgcgtaa actgctgacg ggtgagctgc
     3421 tgaccctggc aagccgccag caactgattg attggatgga agcggataaa gtggcgggtc
     3481 cgctgctgcg tagcgcgctg ccggctggct ggtttattgc ggataaaagc ggtgcgggcg
     3541 aacgtggcag ccgtggcatt attgcggcgc tgggcccgga tggtaaaccg agccgtattg
     3601 tggtgattta taccaccggc agccaggcga cgatggatga acgtaaccgt cagattgcgg
     3661 aaattggcgc gagcctgatt aaacattggt aaaccgatac aattaaaggc tccttttgga
     3721 gccttttttt ttggacgacc cttgtcagat caaaggatct tc
//
