LOCUS       sequence-467557-        2744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Marker_AmpR-bla_PartType4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK012 [Marker_AmpR-bla_PartType4]
FEATURES             Location/Qualifiers
     source          1..2744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        661..752
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(753..821,822..1613)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1617..1656
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     promoter        1722..1813
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1814..1882,1883..2674)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2678..2717
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctcttggca gtagcccgcc taatgagcgg gctttttttt aattccccta
      661 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      721 aaatgcttca ataatattga aaaaggaaga gtatgagcat tcagcatttt cgtgtggcgc
      781 tgattccgtt ttttgcggcg ttttgcctgc cggtgtttgc gcatccggaa accctggtga
      841 aagtgaaaga tgcggaagat caactgggtg cgcgcgtggg ctatattgaa ctggatctga
      901 acagcggcaa aattctggaa tcttttcgtc cggaagaacg ttttccgatg atgagcacct
      961 ttaaagtgct gctgtgcggt gcggttctga gccgtgtgga tgcgggccag gaacaactgg
     1021 gccgtcgtat tcattatagc cagaacgatc tggtggaata tagcccggtg accgaaaaac
     1081 atctgaccga tggcatgacc gtgcgtgaac tgtgcagcgc ggcgattacc atgagcgata
     1141 acaccgcggc gaacctgctg ctgacgacca ttggcggtcc gaaagaactg accgcgtttc
     1201 tgcataacat gggcgatcat gtgacccgtc tggatcgttg ggaaccggaa ctgaacgaag
     1261 cgattccgaa cgatgaacgt gataccacca tgccggcagc aatggcgacc accctgcgta
     1321 aactgctgac gggtgagctg ctgaccctgg caagccgcca gcaactgatt gattggatgg
     1381 aagcggataa agtggcgggt ccgctgctgc gtagcgcgct gccggctggc tggtttattg
     1441 cggataaaag cggtgcgggc gaacgtggca gccgtggcat tattgcggcg ctgggcccgg
     1501 atggtaaacc gagccgtatt gtggtgattt ataccaccgg cagccaggcg acgatggatg
     1561 aacgtaaccg tcagattgcg gaaattggcg cgagcctgat taaacattgg taaaccgata
     1621 caattaaagg ctccttttgg agcctttttt tttggacgac ccttgtcaac gtgagaccag
     1681 agtagcccgc ctaatgagcg ggcttttttt taattcccct atttgtttat ttttctaaat
     1741 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1801 aaaaaggaag agtatgagca ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc
     1861 gttttgcctg ccggtgtttg cgcatccgga aaccctggtg aaagtgaaag atgcggaaga
     1921 tcaactgggt gcgcgcgtgg gctatattga actggatctg aacagcggca aaattctgga
     1981 atcttttcgt ccggaagaac gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg
     2041 tgcggttctg agccgtgtgg atgcgggcca ggaacaactg ggccgtcgta ttcattatag
     2101 ccagaacgat ctggtggaat atagcccggt gaccgaaaaa catctgaccg atggcatgac
     2161 cgtgcgtgaa ctgtgcagcg cggcgattac catgagcgat aacaccgcgg cgaacctgct
     2221 gctgacgacc attggcggtc cgaaagaact gaccgcgttt ctgcataaca tgggcgatca
     2281 tgtgacccgt ctggatcgtt gggaaccgga actgaacgaa gcgattccga acgatgaacg
     2341 tgataccacc atgccggcag caatggcgac caccctgcgt aaactgctga cgggtgagct
     2401 gctgaccctg gcaagccgcc agcaactgat tgattggatg gaagcggata aagtggcggg
     2461 tccgctgctg cgtagcgcgc tgccggctgg ctggtttatt gcggataaaa gcggtgcggg
     2521 cgaacgtggc agccgtggca ttattgcggc gctgggcccg gatggtaaac cgagccgtat
     2581 tgtggtgatt tataccaccg gcagccaggc gacgatggat gaacgtaacc gtcagattgc
     2641 ggaaattggc gcgagcctga ttaaacattg gtaaaccgat acaattaaag gctccttttg
     2701 gagccttttt ttttggacga cccttgtcag atcaaaggat cttc
//
