LOCUS       sequence-467621-        3070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Origin_pBR322-ROP_PartType1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK021 [Origin_pBR322-ROP_PartType1]
FEATURES             Location/Qualifiers
     source          1..3070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      636..1224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1378..1395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1410..1550
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1476..1495)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1636..1658
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1652..1843)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        2048..2139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2140..2208,2209..3000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      3004..3043
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctccgaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      661 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      721 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      781 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      841 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      901 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      961 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1021 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1081 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1141 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1201 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1261 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1321 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1381 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     1441 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     1501 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     1561 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     1621 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     1681 gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg cagcgattca cagatgtctg
     1741 cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga
     1801 taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag
     1861 ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac
     1921 gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg
     1981 tatggatgcg gcgcaattga gaccagagta gcccgcctaa tgagcgggct tttttttaat
     2041 tcccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2101 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagcattca gcattttcgt
     2161 gtggcgctga ttccgttttt tgcggcgttt tgcctgccgg tgtttgcgca tccggaaacc
     2221 ctggtgaaag tgaaagatgc ggaagatcaa ctgggtgcgc gcgtgggcta tattgaactg
     2281 gatctgaaca gcggcaaaat tctggaatct tttcgtccgg aagaacgttt tccgatgatg
     2341 agcaccttta aagtgctgct gtgcggtgcg gttctgagcc gtgtggatgc gggccaggaa
     2401 caactgggcc gtcgtattca ttatagccag aacgatctgg tggaatatag cccggtgacc
     2461 gaaaaacatc tgaccgatgg catgaccgtg cgtgaactgt gcagcgcggc gattaccatg
     2521 agcgataaca ccgcggcgaa cctgctgctg acgaccattg gcggtccgaa agaactgacc
     2581 gcgtttctgc ataacatggg cgatcatgtg acccgtctgg atcgttggga accggaactg
     2641 aacgaagcga ttccgaacga tgaacgtgat accaccatgc cggcagcaat ggcgaccacc
     2701 ctgcgtaaac tgctgacggg tgagctgctg accctggcaa gccgccagca actgattgat
     2761 tggatggaag cggataaagt ggcgggtccg ctgctgcgta gcgcgctgcc ggctggctgg
     2821 tttattgcgg ataaaagcgg tgcgggcgaa cgtggcagcc gtggcattat tgcggcgctg
     2881 ggcccggatg gtaaaccgag ccgtattgtg gtgatttata ccaccggcag ccaggcgacg
     2941 atggatgaac gtaaccgtca gattgcggaa attggcgcga gcctgattaa acattggtaa
     3001 accgatacaa ttaaaggctc cttttggagc cttttttttt ggacgaccct tgtcagatca
     3061 aaggatcttc
//
