LOCUS       sequence-467622-        6118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of N-terminally 6His-tagged N-myc; includes TEV
            cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Yang J, Chung CI, Koach J, Liu H, Navalkar A, He H, Ma Z, Zhao Q, 
            Yang X, He L, Mittag T, Shen Y, Weiss WA, Shu X
  TITLE     MYC phase separation selectively modulates the transcriptome.
  JOURNAL   Nat Struct Mol Biol. 2024 Oct;31(10):1567-1579. doi: 
            10.1038/s41594-024-01322-6. Epub 2024 May 29.
  PUBMED    38811792
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-024-01322-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2024-10"; volume: "31"; issue: "10"; pages: "1567-1579"
COMMENT     Sequence Label: pDest17_N-myc
FEATURES             Location/Qualifiers
     source          1..6118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1367..1558
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1552..1574)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1660..1799
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1715..1734
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1814..1831)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1985..2573)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2065..2084)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2744..3535,3536..3604))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3367..3386
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3605..3709)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3777..3795
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(3822..3827,3828..3844,3845..3850)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(4216..4263)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     4259..4277
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     protein_bind    4330..4354
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5754..5774)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(5777..5801)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5811..5828)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             5848..5870
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(5901..5920)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5902..5920)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6004..6023)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
       61 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgatcgaccg atgcccttga
      121 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
      181 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
      241 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
      301 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
      361 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
      421 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
      481 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
      541 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg
      601 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg
      661 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct
      721 cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc
      781 caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat
      841 cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc
      901 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct
      961 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa
     1021 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct
     1081 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg
     1141 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt
     1201 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag
     1261 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg
     1321 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga
     1381 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa
     1441 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
     1501 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     1561 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     1621 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     1681 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     1741 gtgcaccatt gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     1801 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1861 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1921 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1981 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2041 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2101 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2161 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2221 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2281 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2341 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2401 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     2461 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2521 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2581 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2641 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2701 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2761 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2821 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2881 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2941 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3001 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     3061 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3121 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3181 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3241 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3301 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3361 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3421 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3481 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3541 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3601 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3661 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3721 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     3781 ggcgtatcac gaggcccttt cgtcttcaag aattctcatg tttgacagct tatcatcgat
     3841 aagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc accgtgtatg
     3901 aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata
     3961 ggcttggtta tgccggtact gccgggcctc ttgcgggata tcgtccattc cgacagcatc
     4021 gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct atgcgcaccc
     4081 gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc ttcgctactt
     4141 ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat atccggatat
     4201 agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc
     4261 tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc
     4321 ctcgaatcaa ccactttgta caagaaagct gggtcttagc aggtacgtgc atgttcaatt
     4381 ttcttcagca gttgctgctg acgtgcctgc agtttctctt tttctaacag cagctgatgt
     4441 tcttcggcct gcagactatg aacatattcg gttgcctttt tcaggatcac cactttggct
     4501 gctttttcat ttttaaccag ttccggcaca tgatcacgca gggtcagaaa gctgctacgc
     4561 agatcattac gacgctgacg ttccagaatg ttatgattgc gacgacgttc gctatcttca
     4621 ctatcgctat tacgcggact caggcttttt gctttaggcg gaataacgct tttcagcgga
     4681 cgcggactgg cttcgctttt aatctttttc tgaggcggtg catcttcgct ttcaacatac
     4741 ggactcggtg ctgcataatt atgctgctga tgaatcggca gacaacgttt cagaatcagt
     4801 tcgctgctct gtgcacgacc cggacctaat gcggcatttt tcggacgaac ggtaatggta
     4861 aaggtggtaa ctgctttggt attgctgctg ctacgacgtt tttcaacggt aacaacgtca
     4921 atctcttctt cttcgtcttc ctcttcatca tcctcatcgt cgctatcgct cagggtatct
     4981 tcgcctgagg tgctcagtgc tttgtgatca ccaccgctgg tctgacgacc accaggacgt
     5041 ggaggtgcaa cacccggtgc gccagctggt gctgcaatac ctgcaccgct tgccactgcc
     5101 ggaccagctg ctggtgcact tgccggagct gccggaaccg gtgccggttc acgtttatta
     5161 accggaaacg gaaaaacaac tgccggatca acacattctg ctgccggatg tgccagttct
     5221 gcaggcagtg ctgcacccgc tctacctgcg cctgctgcac caccatgacc gcgacctgcc
     5281 ggacttgctg caccggcacc aggactctgt gcggtgctac ctgcggtagg cggaccacga
     5341 ccatgctgca gtttttcgct aactgcacgt tccagttttt cacgtgcgct aaaacctgac
     5401 cacatacaat cctgcagaat aaccggattc ggggtcagac cacctaagcc acctaaacca
     5461 aatgcatctt cttcggcagg gctaccccac agttcatttt ccagcagcat ttcggtaacc
     5521 cagctaggcg gttcgcttga atgttctgca aaaccacggc tcgggctcag aggcggtgtc
     5581 ggcagcagtt caaacttttt ccagatatct tcacccggag gggtgctatc cggaccacca
     5641 aaatagaaat catcttcatc cggataaaaa cacggctgca ggctatcaaa ttccagatcc
     5701 ggatttttac aaatcatacc aggcatggtg ctggtgctac agctcggcat gctaccctga
     5761 aaatacaggt tctcgaagcc tgcttttttg tacaaacttg ttgattcgag gtgatggtga
     5821 tggtgatggt agtacgacat atgtatatct ccttcttaaa gttaaacaaa attatttcta
     5881 gagggaaacc gttgtggtct ccctatagtg agtcgtatta atttcgcggg atcgagatct
     5941 cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     6001 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     6061 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgc
//
