LOCUS       sequence-467623-       12831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the Dbl homology and pleckstrin homology (DH-PH) domain of 
            Intersectin1 controlled by a Melt variant with a switching 
            temperature of 37(o)C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12831)
  AUTHORS   Benman W, Huang Z, Iyengar P, Wilde D, Mumford TR, Bugaj LJ
  TITLE     A temperature-inducible protein module for control of mammalian cell
            fate.
  JOURNAL   Nat Methods. 2025 Jan 23. doi: 10.1038/s41592-024-02572-4.
  PUBMED    39849131
REFERENCE   2  (bases 1 to 12831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2025 Jan 23. doi: 10.1038/s41592-024-02572-4."
COMMENT     Sequence Label: pLV-PGK-TetOn-Puro_MeltITSN1-37-mch
FEATURES             Location/Qualifiers
     source          1..12831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..388
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    29..47
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    65..83
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    101..119
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    137..155
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    173..191
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    209..227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    245..263
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3300..4010
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3445..3463)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3713..3732
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4246..4380
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    4424..4539
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(4802..4846)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5031..5264
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5761..5886
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5933..6566
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6734..6868
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6916..7020
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7021..7089,7090..7881)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7239..7258)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8052..8640
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8541..8560
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8794..8811
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8928..8949
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8964..8969,8970..8987,8988..8994)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9002..9018
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9007..9029
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9026..9042
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9026..9042
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             9170..9803
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10010..10598
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             10116..10127
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     10525..10545
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10612..11211)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10695..10715)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     11192..11211
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(11220..11549)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11234..11369
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(11560..12306)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(join(12325..12831,1..4))
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(12388..12406)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ccccgtggaa ttatcacctc gagtttactc cctatcagtg atagagaacg tatgaagagt
       61 ttactcccta tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa
      121 cgtataagga gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc
      181 agtgatagag aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt
      241 ttactcccta tcagtgatag agaacgtata agctttgctt atgtaaacca gggcgcctat
      301 aaaagagtgc tgattttttg agtaaacttc aattccacaa cacttttgtc ttataccaac
      361 tttccgtacc acttcctacc ctcgtaaaga attgcggccg cgcgtttaaa atgttgaccc
      421 caactgaaag aaagcgacaa ggatacatcc acgagctcat tgtcaccgag gagaactatg
      481 tgaatgacct gcagctggtc acagagattt ttcaaaaacc cctgatggag tctgagctgc
      541 tgacagaaaa agaggttgct atgatttttg tgaactggaa ggagctgatt atgtgtaata
      601 tcaaactact aaaagcgctg agagtccgca agaagatgtc cggggagaag atgcctgtga
      661 agatgattgg agacatcctg agcgcacagc tgccgcacat gcagccctac atccgcttct
      721 gcagccgcca gctcaacggg gctgccctga tccagcagaa gacggacgag gccccagact
      781 tcaaggagtt cgtcaaaaga ttggcaatgg atcctcggtg taaagggatg ccactctcta
      841 gttttatact gaagcctatg caacgggtaa caagataccc actgatcatt aaaaatatcc
      901 tggaaaacac ccctgaaaac cacccggacc acagccactt gaagcacgcc ctggagaagg
      961 cggaagagct ctgttcccag gtgaacgaag gggtgcggga gaaggagaac tctgaccggc
     1021 tggagtggat ccaggcccac gtgcagtgtg aaggcctgtc tgagcaactt gtgttcaatt
     1081 cagtgaccaa ttgcttgggg ccgcgcaaat ttctgcacag tgggaagctc tacaaggcca
     1141 agagcaacaa ggagctgtat ggcttccttt tcaacgactt cctcctgctg actcagatca
     1201 cgaagccttt ggggtcttct ggcaccgaca aagtcttcag ccccaaatca aacctgcagt
     1261 ataaaatgta taaaacacct attttcctaa atgaggttct agtaaaatta cccaccgacc
     1321 cttctggaga cgagcccatc ttccacatct cccacattga ccgcgtctat actctccgag
     1381 cagaaagcat aaatgaaagg actgcctggg tgcagaaaat caaagctgct tctgaactct
     1441 acatagagac tgagaaaaag aagggtggtg gatcaggtgg aggatccatg gccacagacg
     1501 caatcgacca ttactaccag aacagagacg gccccccttc caggagcttt agcgacgaat
     1561 acgactcata caataagaca gcccctctgc cacctctgag cgcctgccca gagcgcgccc
     1621 agacccctaa cagcaagaga atccccatca cacccaattc cgccaagagc acctccgagt
     1681 ctcctcggag aatcacattc tctcgcgacg caggcagctc caccaagggc agcacccaga
     1741 caagcccatc caaccagggc tccgtgtcta gccggaatgg ctctaacgga aatggcctgg
     1801 gcctgtcctc tgccggcaac ttcgccgatt tctttagctc cgaggtgttt tatatcgtgc
     1861 tgcacaatcc caccacagcc cacagattcc tgaagttttg ccagagcagg gcctgttccg
     1921 agaacctgga gtttctgcag aagatcgacg agtacaatag gctgatcgat gaggccacac
     1981 gcctgctgtc taccatctac agcacatacg tgagcagcga ggcccctaag cagatcaaca
     2041 tctctaatag ccacgccagg cgcctgagcc acgacatcaa gggagcaacc cacaacgtgc
     2101 tgcccggcct ggaggatatc ttcaatggct cccaggagca gatcgagaag ctgctggcct
     2161 ctgacctgta tcctagattt gtgaagcacc aggtgaccgc atccgccaca atggccctgg
     2221 caaacgacaa ggagaggtac ccaggcctgg gcgattgctt ctgtctgaca gacccacgga
     2281 gagccgataa tcccatcgtg ttcgcaagcg acggattcgt gagcgtgacc ggctattccc
     2341 gctctgatat catcccaagg aacgcccgct tcctgcaggg cagctttacc gaccggcagg
     2401 ccacaaagag gctgcgcacc tccatcgaga atcgcgagga gacagtggag ctgctgctga
     2461 actatcggaa gaatggcgat cccttttgga acctgctgta cgtgtcccct ctgctggacg
     2521 gcaatggcga tgtgagattc tttctgggcg gcaatatcaa ctgttctacc acaatccaca
     2581 gccggaccga cgtgctgaga atcctgagcc tgaacgacga ggatacaggc tccttcatcg
     2641 agggctctaa taagaccccc agcgtgcggt ccaaggagtc taacaatgcc tccaactatg
     2701 gcaagacatc cttctttaag tcttttaaga agtacaaggc caataccgtg gagatcagag
     2761 acgaggccgg catggagggc gagctgatcg ataggatcgg caagctgcag ttcaagacac
     2821 aggtggagga gttttacacc gcctattcta agtacctggt gctgagctat cagccacagt
     2881 cccagaacct gatcgtgaag tacttcagcc ccggcatcgt ggatatgctg aacctgaata
     2941 tgcctaatgg cctggtggcc ccaatcgtgg acaaggatat cttcaaggtg ctgacagagc
     3001 actccccttc ctctgtgcca cggaccttta agaacgtggt gagggaaggc atcaaagcag
     3061 gcagggccgt gtctgtggag acaggcctgc tgacaggcat cgaggagatc aagaagtctc
     3121 agaacatctt cagcggcacc acagccaatc gggagagagg ctggaagaag gccgaggaga
     3181 agtacgtgtg ccactttacc ccctgtaagg atgagatggg cagggtcggc tgggtggtgc
     3241 tgactgtcgc tcctaaactg gaaagatttg aattcggtgg tggatcaggt ggaggatcta
     3301 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     3361 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     3421 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     3481 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     3541 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     3601 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     3661 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     3721 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     3781 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     3841 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     3901 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     3961 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa tgatctagag
     4021 ggcccgttta aacccgctga tcagcctcga ctgaccggtg ccggcggatc cagaccacct
     4081 cccctgcgag ctaagctgga cagccaatga cgggtaagag agtgacattt ttcactaacc
     4141 taagacagga gggccgtcag agctactgcc taatccaaag acgggtaaaa gtgataaaaa
     4201 tgtatcactc caacctaaga caggcgcagc ttccgaggga tttgagatcc agacatgata
     4261 agatacattg atgagtttgg acaaaccaaa actagaatgc agtgaaaaaa atgccttatt
     4321 tgtgaaattt gtgatgctat tgccttattt gtaaccatta taagctgcaa taaacaagtt
     4381 aagtcgataa actggatctc tgctgtccct gtaataaacc cgaaaatttt gaatttttgt
     4441 aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc
     4501 ccctgcactg taccccccaa tccccccttt tcttttaaag gcgataccgt cgagatccgt
     4561 tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg
     4621 gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct
     4681 gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc
     4741 ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc
     4801 acaaacttgc ccatttatct aattccaata attcttgttc attcttttct tgctggtttt
     4861 gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact
     4921 ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc
     4981 aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt
     5041 gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga
     5101 ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct
     5161 gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca
     5221 tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt
     5281 tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt
     5341 ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag
     5401 gtctgaagat cagcggccgg ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc
     5461 ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata
     5521 gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg
     5581 ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac
     5641 tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt
     5701 tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact
     5761 gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact
     5821 caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg
     5881 agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag
     5941 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     6001 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     6061 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag
     6121 gcaaaaagca gctgcttata tgcaggatct gagggctcgc cactccccag tcccgcccag
     6181 gccacgcctc cctggaaagt ccccagcgga aagtcccttg tagcaagctc gatatcagca
     6241 gttcttgaag tactccggat gcagctctcg ggccacgtga tgaaatgcta ggcggctgtc
     6301 aaacctccac tctaacactt ctctctccgg gtcatccatc ccatgcaggc tcacagggtg
     6361 taacaagctg gtgttctctc ctttattggc ctcttctacc ttatctggct caactggtac
     6421 tagcttgtag caccatccaa aggtcagtgg atatctgacc cctggccctg gtgtgtagtt
     6481 ctgctaatca gggaagtagc cttgtgtgtg gtagatccac agatcaagga tatcttgtct
     6541 tctttgggag tgaattagcc cttccaacta ctaagtttgt agtacatatt taacaaatac
     6601 aatttcttta aaatgaaaat aattcagagg aatcacaggt ttagagtaaa tgaaaccaca
     6661 ggtaattggc agtggtaata gggtatgggg tgggaagttt gggatgattt tggttagctt
     6721 gagttatcca gttgatccag acatgataag atacattgat gagtttggac aaaccacaac
     6781 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     6841 aaccattata agctgcaata aacaagttga tctcccgatc cgtcgacgtc aggtggcact
     6901 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6961 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7021 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7081 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7141 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7201 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7261 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     7321 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7381 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7441 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7501 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7561 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7621 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7681 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7741 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7801 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7861 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7921 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7981 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8041 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8101 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8161 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     8221 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     8281 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     8341 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     8401 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8461 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8521 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8581 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8641 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8701 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     8761 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8821 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     8881 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     8941 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     9001 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     9061 tagctagagg tcgacggtaa acgctagcaa tgtcaaggcc tctcactctc tgatattcat
     9121 ttctttgcaa gttataaata ctgaataata agatgacatg aactactact gctagagatt
     9181 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     9241 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
     9301 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca
     9361 aaaagcagct gcttatatgc aggatctgag ggctcgccac tccccagtcc cgcccaggcc
     9421 acgcctccct ggaaagtccc cagcggaaag tcccttgtag caagctcgat atcagcagtt
     9481 cttgaagtac tccggatgca gctctcgggc cacgtgatga aatgctaggc ggctgtcaaa
     9541 cctccactct aacacttctc tctccgggtc atccatccca tgcaggctca cagggtgtaa
     9601 caagctggtg ttctctcctt tattggcctc ttctacctta tctggctcaa ctggtactag
     9661 cttgtagcac catccaaagg tcagtggata tctgacccct ggccctggtg tgtagttctg
     9721 ctaatcaggg aagtagcctt gtgtgtggta gatccacaga tcaaggatat cttgtcttcg
     9781 ttgggagtga attagccctt ccagtcccct cttttctttt aaaaagtggc taagatctac
     9841 agctgccttg taagtcattg gtcttaaagg tacctgaggt gtgactggaa aacccacctc
     9901 ctcctcctct tgtgcttcta gccaggcaca atcagcattg gtagctgctg tattgctact
     9961 tgtgattgct ccatgttttt ctaggtctcg actgcagaat taattccagg cggggaggcg
    10021 gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga
    10081 gccggcagca ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag
    10141 aaggacgtcc cgcgcagaat ccaggtggca acacaggcga gcagccatgg aaaggacgtc
    10201 agcttccccg acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag
    10261 cgggcaaggc aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc
    10321 ccggaaagga gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac
    10381 acagtgcaca ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc
    10441 aaccaggatt tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac
    10501 aaaggcatta aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca
    10561 gtcaatcttt cacaaatttt gtaatccaga ggttgattgt tccagacgcg ttcaggcacc
    10621 gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac
    10681 ggtgaagccg agccgctcgt agaaggggag gttgcggggc gcggaggtct ccaggaaggc
    10741 gggcaccccg gcgcgctcgg ccgcctccac tccggggagc acgacggcgc tgcccagacc
    10801 cttgccctgg tggtcgggcg agacgccgac ggtggccagg aaccacgcgg gctccttggg
    10861 ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg gccagccggg aaccgctcaa
    10921 ctcggccatg cgcgggccga tctcggcgaa caccgccccc gcttcgacgc tctccggcgt
    10981 ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac accttgccga tgtcgagccc
    11041 gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc tcgatgtggc ggtccgggtc
    11101 gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg gcgagggtgc gtacggcccg
    11161 ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg tactcggtca tggtcgcgtt
    11221 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
    11281 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
    11341 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct
    11401 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
    11461 cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
    11521 ggggactttc cacaccctaa ctgacacaca ttcgcgcgtt tacccgggga gcatgtcaag
    11581 gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt caagggcatc
    11641 ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg gtcggcccgc cgctttcgca
    11701 ctttagctgt ttctccaggc cacatatgat tagttccagg ccgaaaagga aggcaggttc
    11761 ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga agtgggggca tagaatcggt
    11821 ggtaggtgtc tctctttcct cttttgctac ttgatgctcc tgttcctcca atacgcagcc
    11881 cagtgtaaag tggcccacgg cggacagagc gtacagtgcg ttctccaggg agaagccttg
    11941 ctgacacagg aacgcgagct gattttccag ggtttcgtac tgtttctctg ttgggcgggt
    12001 gccgagatgc actttagccc cgtcgcgatg tgagaggaga gcacagcggt atgacttggc
    12061 gttgttccgc agaaagtctt gccatgactc gccttccagg gggcaggagt gggtatgatg
    12121 cctgtccagc atctcgattg gcagggcatc gagcagggcc cgcttgttct tcacgtgcca
    12181 gtacagggta ggctgctcaa ctcccagctt ttgagcgagt ttccttgtcg tcaggccttc
    12241 gataccgact ccattgagta attccagagc agagtttatg actttgctct tgtccagtct
    12301 agacatggta attcgatgat ccccctgggg agagaggtcg gtgattcggt caacgaggga
    12361 gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg gaacaccgcg cgggcaggaa
    12421 cagggcccac actaccgccc cacaccccgc ctcccgcacc gccccttccc ggccgctgct
    12481 ctcggcgcgc cctgctgagc agccgctatt ggccacagcc catcgcggtc ggcgcgctgc
    12541 cattgctccc tggcgctgtc cgtctgcgag ggtactagtg agacgtgcgg cttccgtttg
    12601 tcacgtccgg cacgccgcga accgcaagga accttcccga cttaggggcg gagcaggaag
    12661 cgtcgccggg gggcccacaa gggtagcggc gaagatccgg gtgacgctgc gaacggacgt
    12721 gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc ccggaaccac gcccagagca
    12781 gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg cgcaacccca a
//
