LOCUS       sequence-467625-       12955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes EGFR intracellular domain controlled by a Melt variant with 
            a switching temperature of 37(o)C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12955)
  AUTHORS   Benman W, Huang Z, Iyengar P, Wilde D, Mumford TR, Bugaj LJ
  TITLE     A temperature-inducible protein module for control of mammalian cell
            fate.
  JOURNAL   Nat Methods. 2025 Jan 23. doi: 10.1038/s41592-024-02572-4.
  PUBMED    39849131
REFERENCE   2  (bases 1 to 12955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2025 Jan 23. doi: 10.1038/s41592-024-02572-4."
COMMENT     Sequence Label: pHR_CMV_MeltEGFR-37-mCherry
FEATURES             Location/Qualifiers
     source          1..12955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    233..350
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        431..734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        735..938
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     889..909
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      976..985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2509..3216
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2654..2672)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2922..2941
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5002..5590
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5055..5075)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5473..5484)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5593..5609)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5594..5610)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5700..5933
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5958..5976)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5959..5976)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6266..6285)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6499..6518)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6618..6640
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6678..6696)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6764..6868
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6869..6937,6938..7729)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7900..8488
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8389..8408
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8642..8659
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8729..8749)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8734..9063
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8914..9049
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10197..10262
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10392..10412
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10837..10971
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10874..10893)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10928..10947
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11139..11772
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11819..11944
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12441..12674
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12859..12903
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
       61 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
      121 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
      181 gattagtgaa cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa
      241 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      301 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
      361 acagggacag cagagatcca gtttggatcg ataagcttga tatcgaattc agcccatata
      421 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      481 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      541 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      601 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      661 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      721 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      781 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      841 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      901 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
      961 gctagcgcta cgcgtgccac catggccggc tctaacggaa atggcctggg cctgtcctct
     1021 gccggcaact tcgccgattt ctttagctcc gaggtgtttt atatcgtgct gcacaatccc
     1081 accacagccc acagattcct gaagttttgc cagagcaggg cctgttccga gaacctggag
     1141 tttctgcaga agatcgacga gtacaatagg ctgatcgatg aggccacacg cctgctgtct
     1201 accatctaca gcacatacgt gagcagcgag gcccctaagc agatcaacat ctctaatagc
     1261 cacgccaggc gcctgagcca cgacatcaag ggagcaaccc acaacgtgct gcccggcctg
     1321 gaggatatct tcaatggctc ccaggagcag atcgagaagc tgctggcctc tgacctgtat
     1381 cctagatttg tgaagcacca ggtgaccgca tccgccacaa tggccctggc aaacgacaag
     1441 gagaggtacc caggcctggg cgattgcttc tgtctgacag acccacggag agccgataat
     1501 cccatcgtgt tcgcaagcga cggattcgtg agcgtgaccg gctattcccg ctctgatatc
     1561 atcccaagga acgcccgctt cctgcagggc agctttaccg accggcaggc cacaaagagg
     1621 ctgcgcacct ccatcgagaa tcgcgaggag acagtggagc tgctgctgaa ctatcggaag
     1681 aatggcgatc ccttttggaa cctgctgtac gtgtcccctc tgctggacgg caatggcgat
     1741 gtgagattct ttctgggcgg caatatcaac tgttctacca caatccacag ccggaccgac
     1801 gtgctgagaa tcctgagcct gaacgacgag gatacaggct ccttcatcga gggctctaat
     1861 aagaccccca gcgtgcggtc caaggagtct aacaatgcct ccaactatgg caagacatcc
     1921 ttctttaagt cttttaagaa gtacaaggcc aataccgtgg agatcagaga cgaggccggc
     1981 atggagggcg agctgatcga taggatcggc aagctgcagt tcaagacaca ggtggaggag
     2041 ttttacaccg cctattctaa gtacctggtg ctgagctatc agccacagtc ccagaacctg
     2101 atcgtgaagt acttcagccc cggcatcgtg gatatgctga acctgaatat gcctaatggc
     2161 ctggtggccc caatcgtgga caaggatatc ttcaaggtgc tgacagagca ctccccttcc
     2221 tctgtgccac ggacctttaa gaacgtggtg agggaaggca tcaaagcagg cagggccgtg
     2281 tctgtggaga caggcctgct gacaggcatc gaggagatca agaagtctca gaacatcttc
     2341 agcggcacca cagccaatcg ggagagaggc tggaagaagg ccgaggagaa gtacgtgtgc
     2401 cactttaccc cctgtaagga tgagatgggc agggtcggct gggtggtgct gactgtcgct
     2461 cctaaactgg aaagatttga attcggtggt ggatcaggtg gaggatctat ggtgagcaag
     2521 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     2581 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     2641 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     2701 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     2761 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     2821 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     2881 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     2941 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     3001 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     3061 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     3121 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     3181 cgccactcca ccggcggcat ggacgagctg tacaagggct cttccgggac aggctccgga
     3241 tccggcacta gtcgaaggcg ccacatcgtt cggaagcgca cgctgcggag gctgctgcag
     3301 gagagggagc ttgtggagcc tcttacaccc agtggagaag ctcccaacca agctctcttg
     3361 aggatcttga aggaaactga attcaaaaag atcaaagtgc tgggctccgg tgcgttcggc
     3421 acggtgtata agggactctg gatcccagaa ggtgagaaag ttaaaattcc cgtcgctatc
     3481 aaggaattaa gagaagcaac atctccgaaa gccaacaagg aaatcctcga tgaagcctac
     3541 gtgatggcca gcgtggacaa cccccacgtg tgccgcctgc tgggcatctg cctcacctcc
     3601 accgtgcaac tcatcacgca gctcatgccc ttcggctgcc tcctggacta tgtccgggaa
     3661 cacaaagaca atattggctc ccagtacctg ctcaactggt gtgtgcagat cgcaaagggc
     3721 atgaactact tggaggaccg tcgcttggtg caccgcgacc tggcagccag gaacgtactg
     3781 gtgaaaacac cgcagcatgt caagatcaca gattttgggc tggccaaact gctgggtgcg
     3841 gaagagaaag aataccatgc agaaggaggc aaagtgccta tcaagtggat ggcattggaa
     3901 tcaattttac acagaatcta tacccaccag agtgatgtct ggagctacgg ggtgaccgtt
     3961 tgggagttga tgacctttgg atccaagcca tatgacggaa tccctgccag cgagatctcc
     4021 tccatcctgg agaaaggaga acgcctccct cagccaccca tatgtaccat cgatgtctac
     4081 atgatcatgg tcaagtgctg gatgatagac gcagatagtc gcccaaagtt ccgtgagttg
     4141 atcatcgaat tctccaaaat ggcccgagac ccccagcgct accttgtcat tcagggggat
     4201 gaaagaatgc atttgccaag tcctacagac tccaacttct accgtgccct gatggatgaa
     4261 gaagacatgg acgacgtggt ggatgccgac gagtacctca tcccacagca gggcttcttc
     4321 agcagcccct ccacgtcacg gactcccctc ctgagctctc tgagtgcaac cagcaacaat
     4381 tccaccgtgg cttgcattga tagaaatggg ctgcaaagct gtcccatcaa ggaagacagc
     4441 ttcttgcagc gatacagctc agaccccaca ggcgccttga ctgaggacag catagacgac
     4501 accttcctcc cagtgcctga atacataaac cagtccgttc ccaaaaggcc cgctggctct
     4561 gtgcagaatc ctgtctatca caatcagcct ctgaaccccg cgcccagcag agacccacac
     4621 taccaggacc cccacagcac tgcagtgggc aaccccgagt atctcaacac tgtccagccc
     4681 acctgtgtca acagcacatt cgacagccct gcccactggg cccagaaagg cagccaccaa
     4741 attagcctgg acaaccctga ctaccagcag gacttctttc ccaaggaagc caagccaaat
     4801 ggcatcttta agggctccac agctgaaaat gcagaatacc taagggtcgc gccacaaagc
     4861 agtgaattta ttggagcata atctagaggg cccgtttaaa cccggcggcc gcaactccca
     4921 cctgcaacat gcgtgactga ctgaggccgc gactctagag tcgacctgca ggcatgcaag
     4981 cttgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     5041 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5101 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5161 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5221 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5281 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5341 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     5401 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     5461 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     5521 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     5581 gcctccccgc atcgataccg tcgacctcga gggaattaat ttgagctcgg tacctttaag
     5641 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     5701 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     5761 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5821 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5881 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta
     5941 gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
     6001 atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct
     6061 ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt
     6121 ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca
     6181 atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
     6241 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     6301 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     6361 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     6421 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     6481 cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     6541 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     6601 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     6661 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     6721 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6781 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6841 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6901 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6961 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7021 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7081 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     7141 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7201 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7261 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7321 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7381 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     7441 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7501 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7561 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7621 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7681 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7741 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7801 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7861 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7921 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7981 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8041 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8101 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8161 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8221 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8281 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8341 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8401 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8461 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8521 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8581 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8641 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     8701 gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     8761 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     8821 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     8881 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8941 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9001 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9061 aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc
     9121 gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc
     9181 gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga
     9241 taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca
     9301 gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat
     9361 tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt
     9421 catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg
     9481 ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc
     9541 ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc
     9601 gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc
     9661 tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa
     9721 tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata
     9781 gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct
     9841 gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg
     9901 cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat
     9961 gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca
    10021 ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat
    10081 tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt
    10141 acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa
    10201 atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt
    10261 agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa
    10321 acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac
    10381 attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat
    10441 tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt
    10501 acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa
    10561 cctttataag taggcataac agttataatc ataacatact gttttttctt actccacaca
    10621 ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa
    10681 tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc
    10741 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    10801 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    10861 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10921 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    10981 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    11041 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    11101 aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca
    11161 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
    11221 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
    11281 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
    11341 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
    11401 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
    11461 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
    11521 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
    11581 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
    11641 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
    11701 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
    11761 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
    11821 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
    11881 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
    11941 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
    12001 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
    12061 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
    12121 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
    12181 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
    12241 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc
    12301 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
    12361 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
    12421 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
    12481 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
    12541 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
    12601 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
    12661 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
    12721 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
    12781 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
    12841 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
    12901 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataa
//
