LOCUS       sequence-467629-       12121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a TEV protease controlled by a Melt variant with a switching
            temperature of 40(o)C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12121)
  AUTHORS   Benman W, Huang Z, Iyengar P, Wilde D, Mumford TR, Bugaj LJ
  TITLE     A temperature-inducible protein module for control of mammalian cell
            fate.
  JOURNAL   Nat Methods. 2025 Jan 23. doi: 10.1038/s41592-024-02572-4.
  PUBMED    39849131
REFERENCE   2  (bases 1 to 12121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2025 Jan 23. doi: 10.1038/s41592-024-02572-4."
COMMENT     Sequence Label: pHR_CMV_MeltTEVp-40-mCherry
FEATURES             Location/Qualifiers
     source          1..12121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    233..350
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        431..734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        735..938
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     889..909
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      976..985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2593..3300
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2738..2756)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3006..3025
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3337..4044
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMN"
     misc_feature    4168..4756
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4221..4241)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4639..4650)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4759..4775)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4760..4776)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4866..5099
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5124..5142)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5125..5142)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5432..5451)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5665..5684)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5784..5806
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5844..5862)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5930..6034
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6035..6103,6104..6895)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6253..6272)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7066..7654
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7555..7574
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7808..7825
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7895..7915)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7900..8229
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8080..8215
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9363..9428
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9558..9578
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10003..10137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10040..10059)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10094..10113
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10305..10938
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10985..11110
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11607..11840
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12025..12069
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
       61 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
      121 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
      181 gattagtgaa cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa
      241 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      301 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
      361 acagggacag cagagatcca gtttggatcg ataagcttga tatcgaattc agcccatata
      421 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      481 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      541 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      601 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      661 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      721 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      781 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      841 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      901 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
      961 gctagcgcta cgcgtgccac catggccggc tctaacggaa atggcctggg cctgtcctct
     1021 gccggcaact tcgccgattt ctttagctcc gaggtgtttt atatcgtgct gcacaatccc
     1081 accacagccc acagattcct gaagttttgc cagagcaggg cctgttccga gaacctggag
     1141 tttctgcaga agatcgacga gtacaatagg ctgatcgatg aggccacacg cctgctgtct
     1201 accatctaca gcacatacgt gagcagcgag gcccctaagc agatcaacat ctctaatagc
     1261 cacgccaggc gcctgagcca cgacatcaag ggagcaaccc acaacgtgct gcccggcctg
     1321 gaggatatct tcaatggctc ccaggagcag atcgagaagc tgctggcctc tgacctgtat
     1381 cctagatttg tgaagcacca ggtgaccgca tccgccacaa tggccctggc aaacgacaag
     1441 gagaggtacc caggcctggg cgattgcttc tgtctgacag acccacggag agccgataat
     1501 cccatcgtgt tcgcaagcga cggattcgtg agcgtgaccg gctattcccg ctctgatatc
     1561 atcccaagga acgcccgctt cctgcagggc agctttaccg accggcaggc cacaaagagg
     1621 ctgcgcacct ccatcgagaa tcgcgaggag acagtggagc tgctgctgaa ctatcggaag
     1681 aatggcgatc ccttttggaa cctgctgtac gtgtcccctc tgctggacgg caatggcgat
     1741 gtgagattct ttctgggcgg caatatcaac tgttctacca caatccacag ccggaccgac
     1801 gtgctgagaa tcctgagcct gaacgacgag gatacaggct ccttcatcga gggctctaat
     1861 aagaccccca gcgtgcggtc caaggagtct aacaatgcct ccaactatgg caagacatcc
     1921 ttctttaagt cttttaagaa gtacaaggcc aataccgtgg agatcagaga cgaggccggc
     1981 atggagggcg agctgatcga taggatcggc aagctgcagt tcaagacaca ggtggaggag
     2041 ttttacaccg cctattctaa gtacctggtg ctgagctatc agccacagtc ccagaacctg
     2101 atcgtgaagt acttcagccc cggcatcgtg gatatgctga acctgaatat gcctaatggc
     2161 ctggtggccc caatcgtgga caaggatatc ttcaaggtgc tgacagagca ctccccttcc
     2221 tctgtgccac ggacctttaa gaacgtggtg agggaaggca tcaaagcagg cagggccgtg
     2281 tctgtggaga caggcctgct gacaggcatc gaggagatca agaagtctca gaacatcttc
     2341 agcggcacca cagccaatcg ggagagaggc tggaagaagg ccgaggagaa gtacgtgtgc
     2401 cactttaccc cctgtaagga tgagatgggc agggtcggct gggtggtgct gactgtcgct
     2461 cctaaactgg aaagatttga attcggtggt ggatcaggtg gaggatctga ttcttcccct
     2521 ggacgaaaga aatttccgtt gaagatcttt aagaaaccac tgaagaaggg tggaggaagc
     2581 ggcggcggtt ctatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     2641 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     2701 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     2761 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     2821 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     2881 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     2941 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     3001 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     3061 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     3121 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     3181 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     3241 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     3301 ggctcttccg ggacaggctc cggatccggc actagtggag aaagcttgtt taaggggccg
     3361 cgtgattaca acccgatatc gagcaccatt tgtcatttga cgaatgaatc tgatgggcac
     3421 acaacatcgt tgtatggtat tggatttggt cccttcatca ttacaaacaa gcacttgttt
     3481 agaagaaata atggaacact gttggtccaa tcactacatg gtgtattcaa ggtcaagaac
     3541 accacgactt tgcaacaaca cctcattgat gggagggaca tgataattat tcgcatgcct
     3601 aaggatttcc caccatttcc tcaaaagctg aaatttagag agccacaaag ggaagagcgc
     3661 atatgtcttg tgacaaccaa cttccaaact aagagcatgt ctagcatggt gtcagacact
     3721 agttgcacat tcccttcatc tgatggcata ttctggaagc attggattca aaccaaggat
     3781 gggcagtgtg gcagtccatt agtatcaact agagatgggt tcattgttgg tatacactca
     3841 gcatcgaatt tcaccaacac aaacaattat ttcacaagcg tgccgaaaaa cttcatggaa
     3901 ttgttgacaa atcaggaggc gcagcagtgg gttagtggtt ggcgattaaa tgctgactca
     3961 gtattgtggg ggggccataa agttttcatg gtgaaacctg aagagccttt tcagccagtt
     4021 aaggaagcga ctcaactcat gaattaatct agagggcccg tttaaacccg gcggccgcaa
     4081 ctcccacctg caacatgcgt gactgactga ggccgcgact ctagagtcga cctgcaggca
     4141 tgcaagcttg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     4201 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     4261 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     4321 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     4381 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     4441 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     4501 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     4561 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     4621 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     4681 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     4741 tgggccgcct ccccgcatcg ataccgtcga cctcgaggga attaatttga gctcggtacc
     4801 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     4861 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     4921 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4981 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5041 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5101 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
     5161 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
     5221 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
     5281 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
     5341 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
     5401 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     5461 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     5521 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     5581 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     5641 gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     5701 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5761 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5821 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5881 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5941 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6001 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     6061 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     6121 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     6181 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6241 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     6301 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6361 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6421 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     6481 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6541 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6601 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6661 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6721 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6781 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6841 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6901 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6961 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     7021 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7081 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7141 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7201 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7261 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7321 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7381 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7441 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7501 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7561 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7621 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7681 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7741 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7801 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     7861 ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga
     7921 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7981 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     8041 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8101 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8161 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8221 ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta
     8281 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
     8341 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
     8401 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
     8461 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
     8521 cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt
     8581 attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt
     8641 aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg
     8701 gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc
     8761 atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa
     8821 gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt
     8881 cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt
     8941 acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga
     9001 aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa
     9061 tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta
     9121 tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga
     9181 cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac
     9241 ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg
     9301 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     9361 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     9421 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     9481 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     9541 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     9601 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     9661 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     9721 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
     9781 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
     9841 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
     9901 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     9961 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    10021 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    10081 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    10141 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    10201 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    10261 taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca
    10321 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
    10381 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
    10441 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
    10501 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
    10561 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
    10621 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
    10681 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
    10741 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
    10801 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    10861 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    10921 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
    10981 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
    11041 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
    11101 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
    11161 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
    11221 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
    11281 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
    11341 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
    11401 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg
    11461 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
    11521 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
    11581 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
    11641 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
    11701 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
    11761 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
    11821 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
    11881 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
    11941 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
    12001 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
    12061 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
    12121 a
//
