LOCUS       sequence-467630-       11633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a Melt variant with a switching temperature of 32(o)C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11633)
  AUTHORS   Benman W, Huang Z, Iyengar P, Wilde D, Mumford TR, Bugaj LJ
  TITLE     A temperature-inducible protein module for control of mammalian cell
            fate.
  JOURNAL   Nat Methods. 2025 Jan 23. doi: 10.1038/s41592-024-02572-4.
  PUBMED    39849131
REFERENCE   2  (bases 1 to 11633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2025 Jan 23. doi: 10.1038/s41592-024-02572-4."
COMMENT     Sequence Label: pHR_CMV_Melt-32-mCherry
FEATURES             Location/Qualifiers
     source          1..11633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    233..350
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        431..734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        735..938
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     889..909
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2872..3582
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3017..3035)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3285..3304
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3680..4268
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3733..3753)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4151..4162)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4271..4287)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4272..4288)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4378..4611
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4636..4654)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4637..4654)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4944..4963)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5177..5196)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5296..5318
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5356..5374)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5442..5546
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5547..5615,5616..6407)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5765..5784)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6578..7166
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7067..7086
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7320..7337
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7407..7427)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7412..7741
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7592..7727
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8875..8940
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9070..9090
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9515..9649
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9552..9571)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9606..9625
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9817..10450
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10497..10622
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11119..11352
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11537..11581
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
       61 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
      121 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
      181 gattagtgaa cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa
      241 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      301 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
      361 acagggacag cagagatcca gtttggatcg ataagcttga tatcgaattc agcccatata
      421 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      481 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      541 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      601 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      661 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      721 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      781 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      841 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      901 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
      961 gctagcgcta cgcgtatggc cacagacgca atcgaccatt actaccagaa cagagacggc
     1021 cccccttcca ggagctttag cgacgaatac gactcataca ataagacagc ccctctgcca
     1081 cctctgagcg cctgcccaga gcgcgcccag acccctaaca gcaagagaat ccccatcaca
     1141 cccaattccg ccaagagcac ctccgagtct cctcggagaa tcacattctc tcgcgacgca
     1201 ggcagctcca ccaagggcag cacccagaca agcccatcca accagggctc cgtgtctagc
     1261 cggaatggct ctaacggaaa tggcctgggc ctgtcctctg ccggcaactt cgccgatttc
     1321 tttagctccg aggtgtttta tatcgtgctg cacaatccca ccacagccca cagattcctg
     1381 aagttttgcc agagcagggc ctgttccgag aacctggagt ttctgcagaa gatcgacgag
     1441 tacaataggc tgatcgatga ggccacacgc ctgctgtcta ccatctacag cacatacgtg
     1501 agcagcgagg cccctaagca gatcaacatc tctaatagcc acgccaggcg cctgagccac
     1561 gacatcaagg gagcaaccca caacgtgctg cccggcctgg aggatatctt caatggctcc
     1621 caggagcaga tcgagaagct gctggcctct gacctgtatc ctagatttgt gaagcaccag
     1681 gtgaccgcat ccgccacaat ggccctggca aacgacaagg agaggtaccc aggcctgggc
     1741 gattgcttct gtctgacaga cccacggaga gccgataatc ccatcgtgtt cgcaagcgac
     1801 ggattcgtga gcgtgaccgg ctattcccgc tctgatatca tcccaaggaa ctgccgcttc
     1861 ctgcagggca gctttaccga ccggcaggcc acaaagaggc tgcgcacctc catcgagaat
     1921 cgcgaggaga cagtggagct gctgctgaac tatcggaaga atggcgatcc cttttggaac
     1981 ctgctgtacg tgtcccctct gctggacggc aatggcgatg tgagattctt tctgggcggc
     2041 aatatcaact gttctaccac aatccacagc cggaccgacg tgctgagaat cctgagcctg
     2101 aacgacgagg atacaggctc cttcatcgag ggctctaata agacccccag cgtgcggtcc
     2161 aaggagtcta acaatgcctc caactatggc aagacatcct tctttaagtc ttttaagaag
     2221 tacaaggcca ataccgtgga gatcagagac gaggccggca tggagggcga gctgatcgat
     2281 aggatcggca agctgcagtt caagacacag gtggaggagt tttacaccgc ctattctaag
     2341 tacctggtgc tgagctatca gccacagtcc cagaacctga tcgtgaagta cttcagcccc
     2401 ggcatcgtgg atatgctgaa cctgaatatg cctaatggcc tggtggcccc aatcgtggac
     2461 aaggatatct tcaaggtgct gacagagcac tccccttcct ctgtgccacg gacctttaag
     2521 aacgtggtga gggaaggcat caaagcaggc agggccgtgt ctgtggagac aggcctgctg
     2581 acaggcatcg aggagatcaa gaagtctcag aacatcttca gcggcaccac agccaatcgg
     2641 gagagaggct ggaagaaggc cgaggagaag tacgtgtgcc actttacccc ctgtaaggat
     2701 gagatgggca gggtcggctg ggtggtgctg actgtcgctc ctaaactgga aagatttgaa
     2761 ttcggtggtg gatcaggtgg aggatctgat tcttcccctg gacgaaagaa atttccgttg
     2821 aagatcttta agaaaccact gaagaagggt ggaggaagcg gcggcggttc tatggtgagc
     2881 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     2941 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     3001 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     3061 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     3121 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     3181 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     3241 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     3301 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     3361 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     3421 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     3481 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     3541 ggccgccact ccaccggcgg catggacgag ctgtacaagt aagcggccgc aactcccacc
     3601 tgcaacatgc gtgactgact gaggccgcga ctctagagtc gacctgcagg catgcaagct
     3661 tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3721 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3781 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3841 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3901 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3961 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4021 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     4081 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4141 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4201 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4261 ctccccgcat cgataccgtc gacctcgagg gaattaattt gagctcggta cctttaagac
     4321 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     4381 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     4441 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4501 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4561 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga
     4621 attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat
     4681 gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg
     4741 cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg
     4801 acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat
     4861 cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg
     4921 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     4981 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     5041 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     5101 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     5161 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     5221 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     5281 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     5341 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     5401 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5461 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5521 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5581 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5641 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5701 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5761 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5821 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5881 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5941 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6001 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6061 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6121 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6181 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6241 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6301 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6361 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6421 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6481 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6541 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6601 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6661 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6721 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6781 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6841 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6901 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6961 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7021 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7081 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7141 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7201 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7261 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7321 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7381 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7441 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     7501 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7561 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7621 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7681 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     7741 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
     7801 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
     7861 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
     7921 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
     7981 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
     8041 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
     8101 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
     8161 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
     8221 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
     8281 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
     8341 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
     8401 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
     8461 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
     8521 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
     8581 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
     8641 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
     8701 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
     8761 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
     8821 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     8881 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     8941 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     9001 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     9061 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     9121 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     9181 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     9241 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     9301 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
     9361 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
     9421 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     9481 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     9541 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9601 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     9661 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     9721 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     9781 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     9841 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     9901 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     9961 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
    10021 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
    10081 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
    10141 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
    10201 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
    10261 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
    10321 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
    10381 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
    10441 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
    10501 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
    10561 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
    10621 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
    10681 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
    10741 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
    10801 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
    10861 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
    10921 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
    10981 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
    11041 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
    11101 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
    11161 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
    11221 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
    11281 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
    11341 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
    11401 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
    11461 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
    11521 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    11581 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taa
//
