LOCUS       sequence-467631-       11476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a protein that localizes to nucleus under the control of a 
            Melt variant with a switching temperature of 40(o)C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11476)
  AUTHORS   Benman W, Huang Z, Iyengar P, Wilde D, Mumford TR, Bugaj LJ
  TITLE     A temperature-inducible protein module for control of mammalian cell
            fate.
  JOURNAL   Nat Methods. 2025 Jan 23. doi: 10.1038/s41592-024-02572-4.
  PUBMED    39849131
REFERENCE   2  (bases 1 to 11476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2025 Jan 23. doi: 10.1038/s41592-024-02572-4."
COMMENT     Sequence Label: pHR_CMV_MeltNLS/NES-40-mCherry
FEATURES             Location/Qualifiers
     source          1..11476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    233..350
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        431..734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        735..938
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     889..909
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      976..985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2593..3300
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2738..2756)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3006..3025
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3310..3330
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3523..4111
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3576..3596)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3994..4005)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4114..4130)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4115..4131)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4221..4454
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4479..4497)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4480..4497)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4787..4806)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5020..5039)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5139..5161
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5199..5217)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5285..5389
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5390..5458,5459..6250)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5608..5627)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6421..7009
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6910..6929
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7163..7180
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7250..7270)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7255..7584
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7435..7570
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8718..8783
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8913..8933
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9358..9492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9395..9414)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9449..9468
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9660..10293
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10340..10465
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10962..11195
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11380..11424
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
       61 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
      121 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
      181 gattagtgaa cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa
      241 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      301 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
      361 acagggacag cagagatcca gtttggatcg ataagcttga tatcgaattc agcccatata
      421 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      481 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      541 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      601 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      661 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      721 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      781 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      841 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      901 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
      961 gctagcgcta cgcgtgccac catggccggc tctaacggaa atggcctggg cctgtcctct
     1021 gccggcaact tcgccgattt ctttagctcc gaggtgtttt atatcgtgct gcacaatccc
     1081 accacagccc acagattcct gaagttttgc cagagcaggg cctgttccga gaacctggag
     1141 tttctgcaga agatcgacga gtacaatagg ctgatcgatg aggccacacg cctgctgtct
     1201 accatctaca gcacatacgt gagcagcgag gcccctaagc agatcaacat ctctaatagc
     1261 cacgccaggc gcctgagcca cgacatcaag ggagcaaccc acaacgtgct gcccggcctg
     1321 gaggatatct tcaatggctc ccaggagcag atcgagaagc tgctggcctc tgacctgtat
     1381 cctagatttg tgaagcacca ggtgaccgca tccgccacaa tggccctggc aaacgacaag
     1441 gagaggtacc caggcctggg cgattgcttc tgtctgacag acccacggag agccgataat
     1501 cccatcgtgt tcgcaagcga cggattcgtg agcgtgaccg gctattcccg ctctgatatc
     1561 atcccaagga acgcccgctt cctgcagggc agctttaccg accggcaggc cacaaagagg
     1621 ctgcgcacct ccatcgagaa tcgcgaggag acagtggagc tgctgctgaa ctatcggaag
     1681 aatggcgatc ccttttggaa cctgctgtac gtgtcccctc tgctggacgg caatggcgat
     1741 gtgagattct ttctgggcgg caatatcaac tgttctacca caatccacag ccggaccgac
     1801 gtgctgagaa tcctgagcct gaacgacgag gatacaggct ccttcatcga gggctctaat
     1861 aagaccccca gcgtgcggtc caaggagtct aacaatgcct ccaactatgg caagacatcc
     1921 ttctttaagt cttttaagaa gtacaaggcc aataccgtgg agatcagaga cgaggccggc
     1981 atggagggcg agctgatcga taggatcggc aagctgcagt tcaagacaca ggtggaggag
     2041 ttttacaccg cctattctaa gtacctggtg ctgagctatc agccacagtc ccagaacctg
     2101 atcgtgaagt acttcagccc cggcatcgtg gatatgctga acctgaatat gcctaatggc
     2161 ctggtggccc caatcgtgga caaggatatc ttcaaggtgc tgacagagca ctccccttcc
     2221 tctgtgccac ggacctttaa gaacgtggtg agggaaggca tcaaagcagg cagggccgtg
     2281 tctgtggaga caggcctgct gacaggcatc gaggagatca agaagtctca gaacatcttc
     2341 agcggcacca cagccaatcg ggagagaggc tggaagaagg ccgaggagaa gtacgtgtgc
     2401 cactttaccc cctgtaagga tgagatgggc agggtcggct gggtggtgct gactgtcgct
     2461 cctaaactgg aaagatttga attcggtggt ggatcaggtg gaggatctga ttcttcccct
     2521 ggacgaaaga aatttccgtt gaagatcttt aagaaaccac tgaagaaggg tggaggaagc
     2581 ggcggcggtt ctatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     2641 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     2701 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     2761 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     2821 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     2881 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     2941 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     3001 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     3061 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     3121 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     3181 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     3241 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     3301 ggctcttccc cgaaaaagaa acgcaaagtt ggcggtggat ctggtggcgg ttctggcatt
     3361 tttggcctgg tgaccaacct ggaagaactg gaagtggatt aatctagagg gcccgtttaa
     3421 acccggcggc cgcaactccc acctgcaaca tgcgtgactg actgaggccg cgactctaga
     3481 gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc tctggattac
     3541 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3601 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3661 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     3721 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     3781 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3841 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3901 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     3961 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4021 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4081 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg agggaattaa
     4141 tttgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     4201 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     4261 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4321 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     4381 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     4441 gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt
     4501 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
     4561 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
     4621 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac
     4681 ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga
     4741 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     4801 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     4861 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     4921 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     4981 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc
     5041 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5101 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5161 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     5221 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5281 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5341 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5401 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5461 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5521 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5581 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5641 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5701 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5761 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5821 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5881 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5941 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6001 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6061 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6121 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6181 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6241 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6301 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6361 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6421 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6481 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6541 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6601 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6661 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6721 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6781 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6841 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6901 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6961 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7021 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7081 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7141 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7201 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
     7261 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7321 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7381 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7441 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7501 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7561 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     7621 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     7681 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     7741 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     7801 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     7861 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     7921 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     7981 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     8041 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     8101 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     8161 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     8221 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     8281 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     8341 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     8401 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     8461 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     8521 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     8581 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     8641 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     8701 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     8761 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     8821 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     8881 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     8941 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     9001 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     9061 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     9121 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     9181 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     9241 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     9301 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     9361 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     9421 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     9481 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     9541 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     9601 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     9661 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     9721 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     9781 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     9841 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     9901 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     9961 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
    10021 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
    10081 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
    10141 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
    10201 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    10261 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
    10321 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
    10381 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
    10441 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
    10501 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
    10561 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
    10621 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
    10681 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
    10741 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
    10801 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
    10861 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
    10921 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
    10981 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
    11041 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
    11101 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
    11161 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
    11221 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
    11281 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
    11341 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
    11401 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
    11461 ataaaattat tcataa
//
