LOCUS       sequence-467639-        3662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Origin_pRO1600-ColE1_PartType1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK022 [Origin_pRO1600-ColE1_PartType1]
FEATURES             Location/Qualifiers
     source          1..3662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      663..1014
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             1028..1861
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      complement(1915..2503)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2640..2731
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2732..2800,2801..3592)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      3596..3635
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctccgat ctcaggcgcc gctggtgccg ctggttggac gccaagggtg
      661 aatccgcctc gataccctga ttactcgctt cctgcgccct ctcaggcggc gataggggac
      721 tggtaaaacg gggattgccc agacgcctcc cccgcccctt caggggcaca aatgcggccc
      781 caacggggcc acgtagtggt gcgttttttg cgtttccacc cttttcttcc ttttcccttt
      841 taaacctttt aggacgtcta caggccacgt aatccgtggc ctgtagagtt taaaaaggga
      901 cggatttgtt gccattaagg gacggatttg ttgttaagaa gggacggatt tgttgttgta
      961 aagggacgga tttgttgtat tgtgggacgc agatacagtg tccccttata cacaaggaat
     1021 gtcgaacgtg gcctcacccc caatggttta caaaagcaat gccctggtcg aggccgcgta
     1081 tcgcctcagt gttcaggaac agcggatcgt tctggcctgt attagccagg tgaagaggag
     1141 cgagcctgtc accgatgaag tgatgtattc agtgacggcg gaggacatag cgacgatggc
     1201 gggtgtccct atcgaatctt cctacaacca gctcaaagaa gcggccctgc gcctgaaacg
     1261 gcgggaagtc cggttaaccc aagagcccaa tggcaagggg aaaagaccga gtgtgatgat
     1321 taccggctgg gtgcaaacaa tcatctaccg ggagggtgag ggccgtgtag aactcaggtt
     1381 caccaaagac atgctgccgt acctgacgga actcaccaaa cagttcacca aatacgcctt
     1441 ggctgacgtg gccaagatgg acagcaccca cgcgatcagg ctttacgagc tgctcatgca
     1501 atgggacagc atcggccagc gcgaaataga aattgaccag ctgcgaaagt ggtttcaact
     1561 ggaaggccgg tatccctcga tcaaggactt caagttgcga gtgcttgatc cagccgtgac
     1621 gcagatcaac gagcacagcc cgctacaggt ggagtgggcg cagcgaaaga ccgggcgcaa
     1681 ggtcacacat ctgttgttca gttttggacc gaagaagccc gccaaggcgg tgggtaaggc
     1741 cccagcgaag cgcaaggccg ggaagatttc agatgctgag atcgcgaaac aggctcgccc
     1801 tggtgagaca tgggaagcgg cccgcgctcg actaacccag atgccgctgg atctggccta
     1861 gaggccgtgg ccaccacgga ccggcctgcc tttcacggcc gcgttgctgg cgtttttcca
     1921 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1981 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2041 tgttccgacc ctgccgctta ccggatgcct gtccgccttt ctcccttcgg gaagcgtggc
     2101 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2161 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2221 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2281 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2341 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2401 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2461 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2521 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2581 attatcaatt gagaccagag tagcccgcct aatgagcggg ctttttttta attcccctat
     2641 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2701 aatgcttcaa taatattgaa aaaggaagag tatgagcatt cagcattttc gtgtggcgct
     2761 gattccgttt tttgcggcgt tttgcctgcc ggtgtttgcg catccggaaa ccctggtgaa
     2821 agtgaaagat gcggaagatc aactgggtgc gcgcgtgggc tatattgaac tggatctgaa
     2881 cagcggcaaa attctggaat cttttcgtcc ggaagaacgt tttccgatga tgagcacctt
     2941 taaagtgctg ctgtgcggtg cggttctgag ccgtgtggat gcgggccagg aacaactggg
     3001 ccgtcgtatt cattatagcc agaacgatct ggtggaatat agcccggtga ccgaaaaaca
     3061 tctgaccgat ggcatgaccg tgcgtgaact gtgcagcgcg gcgattacca tgagcgataa
     3121 caccgcggcg aacctgctgc tgacgaccat tggcggtccg aaagaactga ccgcgtttct
     3181 gcataacatg ggcgatcatg tgacccgtct ggatcgttgg gaaccggaac tgaacgaagc
     3241 gattccgaac gatgaacgtg ataccaccat gccggcagca atggcgacca ccctgcgtaa
     3301 actgctgacg ggtgagctgc tgaccctggc aagccgccag caactgattg attggatgga
     3361 agcggataaa gtggcgggtc cgctgctgcg tagcgcgctg ccggctggct ggtttattgc
     3421 ggataaaagc ggtgcgggcg aacgtggcag ccgtggcatt attgcggcgc tgggcccgga
     3481 tggtaaaccg agccgtattg tggtgattta taccaccggc agccaggcga cgatggatga
     3541 acgtaaccgt cagattgcgg aaattggcgc gagcctgatt aaacattggt aaaccgatac
     3601 aattaaaggc tccttttgga gccttttttt ttggacgacc cttgtcagat caaaggatct
     3661 tc
//
