LOCUS       sequence-467640-       11573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a Melt variant with a switching temperature of 37(o)C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11573)
  AUTHORS   Benman W, Huang Z, Iyengar P, Wilde D, Mumford TR, Bugaj LJ
  TITLE     A temperature-inducible protein module for control of mammalian cell
            fate.
  JOURNAL   Nat Methods. 2025 Jan 23. doi: 10.1038/s41592-024-02572-4.
  PUBMED    39849131
REFERENCE   2  (bases 1 to 11573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2025 Jan 23. doi: 10.1038/s41592-024-02572-4."
COMMENT     Sequence Label: pHR_CMV_Melt-37-mCherry
FEATURES             Location/Qualifiers
     source          1..11573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    233..350
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        431..734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        735..938
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     889..909
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      991..1000
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2809..3522
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKG"
     primer_bind     complement(2954..2972)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3222..3241
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3620..4208
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3673..3693)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4091..4102)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4211..4227)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4212..4228)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4318..4551
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4576..4594)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4577..4594)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4884..4903)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5117..5136)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5236..5258
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5296..5314)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5382..5486
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5487..5555,5556..6347)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5705..5724)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6518..7106
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7007..7026
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7260..7277
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7347..7367)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7352..7681
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7532..7667
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8815..8880
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9010..9030
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9455..9589
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9492..9511)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9546..9565
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9757..10390
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10437..10562
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11059..11292
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11477..11521
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
       61 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
      121 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
      181 gattagtgaa cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa
      241 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      301 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
      361 acagggacag cagagatcca gtttggatcg ataagcttga tatcgaattc agcccatata
      421 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      481 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      541 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      601 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      661 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      721 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      781 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      841 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      901 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
      961 gctagcgcta cgcgtcccaa gctggaattc gccaccatgg ccacagacgc aatcgaccat
     1021 tactaccaga acagagacgg ccccccttcc aggagcttta gcgacgaata cgactcatac
     1081 aataagacag cccctctgcc acctctgagc gcctgcccag agcgcgccca gacccctaac
     1141 agcaagagaa tccccatcac acccaattcc gccaagagca cctccgagtc tcctcggaga
     1201 atcacattct ctcgcgacgc aggcagctcc accaagggca gcacccagac aagcccatcc
     1261 aaccagggct ccgtgtctag ccggaatggc tctaacggaa atggcctggg cctgtcctct
     1321 gccggcaact tcgccgattt ctttagctcc gaggtgtttt atatcgtgct gcacaatccc
     1381 accacagccc acagattcct gaagttttgc cagagcaggg cctgttccga gaacctggag
     1441 tttctgcaga agatcgacga gtacaatagg ctgatcgatg aggccacacg cctgctgtct
     1501 accatctaca gcacatacgt gagcagcgag gcccctaagc agatcaacat ctctaatagc
     1561 cacgccaggc gcctgagcca cgacatcaag ggagcaaccc acaacgtgct gcccggcctg
     1621 gaggatatct tcaatggctc ccaggagcag atcgagaagc tgctggcctc tgacctgtat
     1681 cctagatttg tgaagcacca ggtgaccgca tccgccacaa tggccctggc aaacgacaag
     1741 gagaggtacc caggcctggg cgattgcttc tgtctgacag acccacggag agccgataat
     1801 cccatcgtgt tcgcaagcga cggattcgtg agcgtgaccg gctattcccg ctctgatatc
     1861 atcccaagga acgcccgctt cctgcagggc agctttaccg accggcaggc cacaaagagg
     1921 ctgcgcacct ccatcgagaa tcgcgaggag acagtggagc tgctgctgaa ctatcggaag
     1981 aatggcgatc ccttttggaa cctgctgtac gtgtcccctc tgctggacgg caatggcgat
     2041 gtgagattct ttctgggcgg caatatcaac tgttctacca caatccacag ccggaccgac
     2101 gtgctgagaa tcctgagcct gaacgacgag gatacaggct ccttcatcga gggctctaat
     2161 aagaccccca gcgtgcggtc caaggagtct aacaatgcct ccaactatgg caagacatcc
     2221 ttctttaagt cttttaagaa gtacaaggcc aataccgtgg agatcagaga cgaggccggc
     2281 atggagggcg agctgatcga taggatcggc aagctgcagt tcaagacaca ggtggaggag
     2341 ttttacaccg cctattctaa gtacctggtg ctgagctatc agccacagtc ccagaacctg
     2401 atcgtgaagt acttcagccc cggcatcgtg gatatgctga acctgaatat gcctaatggc
     2461 ctggtggccc caatcgtgga caaggatatc ttcaaggtgc tgacagagca ctccccttcc
     2521 tctgtgccac ggacctttaa gaacgtggtg agggaaggca tcaaagcagg cagggccgtg
     2581 tctgtggaga caggcctgct gacaggcatc gaggagatca agaagtctca gaacatcttc
     2641 agcggcacca cagccaatcg ggagagaggc tggaagaagg ccgaggagaa gtacgtgtgc
     2701 cactttaccc cctgtaagga tgagatgggc agggtcggct gggtggtgct gactgtcgct
     2761 cctaaactgg aaagatttga attcggtggt ggatcaggtg gaggatctat ggtgagcaag
     2821 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     2881 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     2941 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     3001 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     3061 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     3121 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     3181 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     3241 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     3301 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     3361 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     3421 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     3481 cgccactcca ccggcggcat ggacgagctg tacaagggct aagcggccgc aactcccacc
     3541 tgcaacatgc gtgactgact gaggccgcga ctctagagtc gacctgcagg catgcaagct
     3601 tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3661 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3721 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3781 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3841 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3901 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3961 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     4021 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4081 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4141 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4201 ctccccgcat cgataccgtc gacctcgagg gaattaattt gagctcggta cctttaagac
     4261 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     4321 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     4381 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4441 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4501 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga
     4561 attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat
     4621 gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg
     4681 cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg
     4741 acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat
     4801 cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg
     4861 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     4921 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     4981 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     5041 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     5101 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     5161 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     5221 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     5281 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     5341 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5401 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5461 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5521 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5581 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5641 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5701 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5761 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5821 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5881 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5941 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6001 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6061 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6121 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6181 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6241 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6301 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6361 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6421 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6481 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6541 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6601 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6661 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6721 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6781 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6841 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6901 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6961 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7021 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7081 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7141 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7201 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7261 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7321 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7381 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     7441 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7501 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7561 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7621 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     7681 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
     7741 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
     7801 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
     7861 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
     7921 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
     7981 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
     8041 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
     8101 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
     8161 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
     8221 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
     8281 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
     8341 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
     8401 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
     8461 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
     8521 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
     8581 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
     8641 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
     8701 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
     8761 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     8821 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     8881 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     8941 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     9001 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     9061 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     9121 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     9181 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     9241 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
     9301 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
     9361 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     9421 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     9481 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9541 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     9601 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     9661 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     9721 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     9781 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     9841 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     9901 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     9961 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
    10021 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
    10081 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
    10141 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
    10201 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
    10261 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
    10321 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
    10381 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
    10441 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
    10501 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
    10561 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
    10621 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
    10681 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
    10741 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
    10801 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
    10861 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
    10921 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
    10981 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
    11041 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
    11101 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
    11161 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
    11221 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
    11281 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
    11341 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
    11401 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
    11461 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    11521 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taa
//
