LOCUS       sequence-467643-       11549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a Melt variant with a switching temperature of 30(o)C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11549)
  AUTHORS   Benman W, Huang Z, Iyengar P, Wilde D, Mumford TR, Bugaj LJ
  TITLE     A temperature-inducible protein module for control of mammalian cell
            fate.
  JOURNAL   Nat Methods. 2025 Jan 23. doi: 10.1038/s41592-024-02572-4.
  PUBMED    39849131
REFERENCE   2  (bases 1 to 11549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2025 Jan 23. doi: 10.1038/s41592-024-02572-4."
COMMENT     Sequence Label: pHR_CMV_Melt-30-mCherry
FEATURES             Location/Qualifiers
     source          1..11549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    233..350
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        431..734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        735..938
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     889..909
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2788..3498
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2933..2951)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3201..3220
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3596..4184
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3649..3669)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4067..4078)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4187..4203)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4188..4204)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4294..4527
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4552..4570)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4553..4570)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4860..4879)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5093..5112)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5212..5234
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5272..5290)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5358..5462
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5463..5531,5532..6323)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5681..5700)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6494..7082
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6983..7002
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7236..7253
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7323..7343)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7328..7657
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7508..7643
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8791..8856
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8986..9006
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9431..9565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9468..9487)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9522..9541
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9733..10366
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10413..10538
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11035..11268
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11453..11497
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
       61 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
      121 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
      181 gattagtgaa cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa
      241 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      301 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
      361 acagggacag cagagatcca gtttggatcg ataagcttga tatcgaattc agcccatata
      421 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      481 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      541 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      601 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      661 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      721 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      781 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      841 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      901 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
      961 gctagcgcta cgcgtatggc cacagacgca atcgaccatt actaccagaa cagagacggc
     1021 cccccttcca ggagctttag cgacgaatac gactcataca ataagacagc ccctctgcca
     1081 cctctgagcg cctgcccaga gcgcgcccag acccctaaca gcaagagaat ccccatcaca
     1141 cccaattccg ccaagagcac ctccgagtct cctcggagaa tcacattctc tcgcgacgca
     1201 ggcagctcca ccaagggcag cacccagaca agcccatcca accagggctc cgtgtctagc
     1261 cggaatggct ctaacggaaa tggcctgggc ctgtcctctg ccggcaactt cgccgatttc
     1321 tttagctccg aggtgtttta tatcgtgctg cacaatccca ccacagccca cagattcctg
     1381 aagttttgcc agagcagggc ctgttccgag aacctggagt ttctgcagaa gatcgacgag
     1441 tacaataggc tgatcgatga ggccacacgc ctgctgtcta ccatctacag cacatacgtg
     1501 agcagcgagg cccctaagca gatcaacatc tctaatagcc acgccaggcg cctgagccac
     1561 gacatcaagg gagcaaccca caacgtgctg cccggcctgg aggatatctt caatggctcc
     1621 caggagcaga tcgagaagct gctggcctct gacctgtatc ctagatttgt gaagcaccag
     1681 gtgaccgcat ccgccacaat ggccctggca aacgacaagg agaggtaccc aggcctgggc
     1741 gattgcttct gtctgacaga cccacggaga gccgataatc ccatcgtgtt cgcaagcgac
     1801 ggattcgtga gcgtgaccgg ctattcccgc tctgatatca tcccaaggaa ctgccgcttc
     1861 ctgcagggca gctttaccga ccggcaggcc acaaagaggc tgcgcacctc catcgagaat
     1921 cgcgaggaga cagtggagct gctgctgaac tatcggaaga atggcgatcc cttttggaac
     1981 ctgctgtacg tgtcccctct gctggacggc aatggcgatg tgagattctt tctgggcggc
     2041 aatatcaact gttctaccac aatccacagc cggaccgacg tgctgagaat cctgagcctg
     2101 aacgacgagg atacaggctc cttcatcgag ggctctaata agacccccag cgtgcggtcc
     2161 aaggagtcta acaatgcctc caactatggc aagacatcct tctttaagtc ttttaagaag
     2221 tacaaggcca ataccgtgga gatcagagac gaggccggca tggagggcga gctgatcgat
     2281 aggatcggca agctgcagtt caagacacag gtggaggagt tttacaccgc ctattctaag
     2341 tacctggtgc tgagctatca gccacagtcc cagaacctga tcgtgaagta cttcagcccc
     2401 ggcatcgtgg atatgctgaa cctgaatatg cctaatggcc tggtggcccc aatcgtggac
     2461 aaggatatct tcaaggtgct gacagagcac tccccttcct ctgtgccacg gacctttaag
     2521 aacgtggtga gggaaggcat caaagcaggc agggccgtgt ctgtggagac aggcctgctg
     2581 acaggcatcg aggagatcaa gaagtctcag aacatcttca gcggcaccac agccaatcgg
     2641 gagagaggct ggaagaaggc cgaggagaag tacgtgtgcc actttacccc ctgtaaggat
     2701 gagatgggca gggtcggctg ggtggtgctg actgtcgctc ctaaactgga aagatttgaa
     2761 ttcggtggtg gatcaggtgg aggatctatg gtgagcaagg gcgaggagga taacatggcc
     2821 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     2881 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     2941 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     3001 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     3061 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     3121 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     3181 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     3241 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     3301 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     3361 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     3421 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     3481 gacgagctgt acaagtaagc ggccgcaact cccacctgca acatgcgtga ctgactgagg
     3541 ccgcgactct agagtcgacc tgcaggcatg caagcttgat atcaagctta tcgataatca
     3601 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3661 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3721 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3781 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3841 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3901 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3961 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     4021 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     4081 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     4141 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     4201 tcgagggaat taatttgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     4261 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     4321 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     4381 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4441 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4501 tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg
     4561 tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa
     4621 cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc
     4681 tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa
     4741 cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat
     4801 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     4861 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     4921 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     4981 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     5041 cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct
     5101 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     5161 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     5221 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     5281 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     5341 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5401 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5461 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5521 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5581 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5641 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5701 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5761 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5821 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5881 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5941 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6001 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6061 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6121 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6181 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6241 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6301 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6361 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6421 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6481 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6541 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6601 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6661 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6721 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6781 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6841 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6901 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6961 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7021 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7081 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7141 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7201 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7261 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7321 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7381 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     7441 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     7501 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7561 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7621 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt
     7681 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     7741 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     7801 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     7861 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     7921 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc
     7981 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
     8041 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
     8101 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga
     8161 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
     8221 tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg
     8281 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
     8341 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg
     8401 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
     8461 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc
     8521 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
     8581 gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg
     8641 aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt
     8701 atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     8761 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     8821 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     8881 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     8941 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     9001 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
     9061 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
     9121 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
     9181 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
     9241 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
     9301 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
     9361 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     9421 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9481 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9541 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
     9601 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
     9661 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
     9721 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
     9781 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     9841 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     9901 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     9961 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
    10021 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
    10081 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
    10141 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
    10201 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    10261 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    10321 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
    10381 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
    10441 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
    10501 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
    10561 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
    10621 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
    10681 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
    10741 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
    10801 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
    10861 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
    10921 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
    10981 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
    11041 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
    11101 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
    11161 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
    11221 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
    11281 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
    11341 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
    11401 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
    11461 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
    11521 ttggctgtgg tatataaaat tattcataa
//
