LOCUS       sequence-467644-        6360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Origin_repABC-p42e_PartType3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 6360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK029 [Origin_repABC-p42e_PartType3]
FEATURES             Location/Qualifiers
     source          1..6360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5338..5429
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5430..5498,5499..6290)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      6294..6333
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctccctt tcagttacga tagagttcca cgagaagtta gtggcccgag
      661 atcaattctc catagatgtc ggcgatctcg ccgtgccagg catcgacact ttgtgtgtag
      721 gttctgtctg cggtctgcct ttgcggcacc tggaattcca cggttcggac agctggccga
      781 gtgacgtagc gctcgcagat ctcccgcctg atctcggcga gctcttcctt gtcggcgccc
      841 ttgagcggca ggaagatcac ctcggcggcg ttcatcgctt tgatcgcctg catagtgacg
      901 tgctccggat tgcccgtgcc gattccgatg atgttaatat gcttcaagcg gatatttccc
      961 cgtgggtcca agcggtcttg ccgaaaaacc gggctggccg caagggcggg gcaggaagtt
     1021 ggccgcggcc aactcggccc gccgtggcgg cttgacccct attgttgccg taaatcccga
     1081 atcgtgtcac tttcgatacg ctttttgacg ctatgttgac agaaaagcgc atcgaaaagg
     1141 attgggtgtt ttggacaaca ttagcgtatc gacgacagac gtgcggatcg agcggcatgc
     1201 gaaccagctc tcgcggcaac tgaagcttct ccgcgacaag ctgtttccgc cgctttcgca
     1261 gaagacgctg cgcacattct cttccggcga agccgcccag atgatcggcg tttccgacgg
     1321 ttatcttcgt cagctttcgc tggacggcaa ggggccgcag ccggaacttt cctcgaatgg
     1381 tcgacgctcc tatacgctcg gtcagatcaa tgaattgcgc cattatatgg cgaatctcaa
     1441 gccgaaggat gcgctgagct acctcccgtg gcgacgtcga ggcgaaaagc tgcagacggt
     1501 cgccgtcacc aatttcaagg gcggttcggc caagacgacg acgacgctct atctggcgca
     1561 atatctggcg ctggagggtt accgggtgct ggcgattgat ctcgacccgc aggcctcgct
     1621 ttcctccatg ctgggcgttc agccggaatt cgatctttcc gacggcgata cgctttatgg
     1681 cgccattcgc tacgatgcgg cccgtaagtc gctgaaggag atcgttcgca agacctattt
     1741 cgatggactc gatctggtgc ccggcaatct cgagctgatg gaattcgagc atgagacgcc
     1801 gcgggcgctc agtgatcgcc agaaaccggc cgagctgttt ttccgtcgcg tcggtgtcgc
     1861 tatcgccgag gtggaggccg attacgatgt ggtggtgatc gactgtccgc cgcagctcgg
     1921 ctacctcact ctcggcgccg tttgcgccgc cacgtcgctg ctcatcacca tccacccgca
     1981 gatggtcgat gtcgcctcta tgtcgcagtt cctgctgatg acctcggatc ttctctctgt
     2041 cgttcgcagg gccggcggcg acctgcagca cgacttcatc aaatatgtcg tcacccgcca
     2101 cgaacccttc gatgcgccgc agtcgcagat cgttgcgctg ctgcgcagcc tgttcggcga
     2161 cgatgtgctg acggcaacca ttctcaaatc gacggcaatc gccgatgccg gtctgacaaa
     2221 gcagacgctt tacgaaatcg agaaagggca ggtgcgccgc tcgacatatg accgggcgct
     2281 ggaatcggtc aacgctgcca atggcgaggt tcttgccggc attcacaaag cctggggccg
     2341 cacatgagca gacgcgatcg cctgaaaggt cttttcgacg atacggcgca ggagttggcc
     2401 gcggccaact acgaggagcc ttcttcgcgc ggatcggccg gtccggtgcg gacaatggcg
     2461 ctgaccctcg gccgcatgga ggaagagagc agggcgctgc aggaggcttt gctctccggc
     2521 gagcgcatcg tcgaactcga tcccgatctg atcgattctt ccttcgtccg cgaccgcctt
     2581 gccgaccagc cgctcgatct tgaggatgag ctggtgcagt cgattgccga caatggccag
     2641 gaagtgccga tccttgttcg gcgccatccc gaagacggtg agcgttacca gatcgcctac
     2701 ggccatcgcc ggctacaggc tatcaagctg ctcaagcgca aggtgcaggc gatcgtccgt
     2761 cagctcgacg acaccgatgt cgttatcgcg cagggcatcg agaattcggc gcgccggaat
     2821 ctttcttata tcgaacgcgc cgtctttgct ctcactctcg aactcaaggg ctttgagcgt
     2881 ccggtcatca tgaaggcgct gtccactgat aagacggagc tgtcgaagct gttgtcggtc
     2941 gccaaggcca ttccggccga gatcgtcagg tcggtggggg ccgcaccggg tatcggacga
     3001 cgcaagtgga tggcacttgc tcaggactgg aatgggacga cggcggcgcg gcttgcgaag
     3061 ctgatcgcct cggatgggtt cagggcggag gagagtgacc gccggttcga acttctggtc
     3121 gccgaactcg ccaggaaaga ggcgaagccg gagcccaccg aatatgactg gaagccgaag
     3181 agcggcggca agattgccgg ccgaatcagg agcgccggca attcctttac aatcgcgttg
     3241 aagaccggcg atgcgccgga tttcggcgct tacatttcac gccgtctcga tgagctttac
     3301 gaagcctatc aggccggcaa gttgcaagca ggagagtagg ccgcaaaaga aaaaagcctc
     3361 cgaacgttgc cgtcgcggaa gcctttctca tatctggaca atctgagaat cgcacttcct
     3421 cgaatcagtg tcaagcatct tcggcatcct tttgggtggt agatttcttt tgccttgaaa
     3481 aaaggtgagg gacatggagc ctcaatatgt atcgacgccc ttcgggcggc ggtcgatgac
     3541 gcttggcatg ctggcaagtc aggaaagggc cagcaggatc gatccggacg cgtcggtcga
     3601 caaatggaag atctttcgcg cgctctgcga agcaaaagac gtgatcggcg tgtccgaccg
     3661 cgcgcttgcc gttctcaacg cgctgttgac cttctatccg aagaacgaga ttaccgaagc
     3721 caacggcttc gtcgtctttc cttcgaatga gcagctgtct ctgcgcgccc atggcatggc
     3781 cggcaccacg ctgcggcgaa acctggcaat gctggtggaa gccggcctga tcatccggcg
     3841 ggacagtccg aatggaaaac gttttgcccg ccgcaacggc gagggcggac ttggggaagc
     3901 cttcggcttc agcctggcgc cactcctcgt gcgtgctggc gagatcgaag ctcaggcggc
     3961 gcaggtgctt gccgacaagc tcgaatggaa gcgcctgagg gagcggctga gcctttgccg
     4021 gcgtgatatc gccaagctca tcgagatcgc gctggaagag gaaattgccg gcgaatggat
     4081 cgagatgcag cagcatttca atcgcctctc gacaagcctg ccgcgccgcc catcgacggc
     4141 ggagatccag agcctgctga ccgatctcga agcgttccgc gaactgatca tcaaaacgct
     4201 ggaatcgaag acgaaagcag aaaaaacaga cgccaatgac agccagagcg gttggcacat
     4261 acataattca aacccacacc acacatctga acttgaacca agcttcgaaa cgaagcaggg
     4321 cgcaaagccg gaggaagaac cgcggccgtg gcgggagccg ccgaaatcct tcccccttgc
     4381 catggtgctg caggcctgcc ccgagattgt tgcctatggg ccgggcggcg ggatcggcag
     4441 ctggcgggat ttgatggccg ccgccgtcgt cgtccgttcc accctaggcg tcagtcccag
     4501 cgcctatcag caggccagcg atgtcatggg accggaaaat gctgccacag tgatcgcctg
     4561 cattctggaa cgcggcggcc atatcaattc ggcgggcggc tatatgcgtg atctgacgcg
     4621 gcgggcagag cgcggtgaat tctcgctcgg tccgatgctc atggcgttga tgcgggccaa
     4681 tggtccgatg gcgagaaaaa cgggatgaaa ggcccgaaag ctttgcgcca gtcgcagctg
     4741 ttagggggat tgtagaacgg cagtcggcca tcttcttctc ttccttcccg cgcttttgtt
     4801 gaaataaacg gttaaccatg tcatagatgg cggaaggaaa cagccgcttt gcttccattt
     4861 gtgagaaact ataaagctta acaatgcgct acgatatcga cggtgtaatg ctccaaaccc
     4921 tgcttccatt gcttgccgcg gcggaggacg cggcggcgcg gctggacgag cgggtgttgc
     4981 gctcatcggt tggtgaggga tttgccgagc gcagccactt cttcgatgcc gcaggtgcgt
     5041 tatgggtcgc cggcgagctc gttcatgtcg aggatctcgt tctgcacgac gcgcatatgg
     5101 atagccgcgc tcccagccat gaactgacga tcgcccattc ggtgctgcgg acgcgtcggc
     5161 gcatctggac cggcgaaccg tcctgggcgc tcggcgcctc ggggctggca acgctgacgg
     5221 cgaccacagg ggaaggggag gggctcactc actggattgt atcaacggac cagtgacagt
     5281 ttctggctga gaccagagta gcccgcctaa tgagcgggct tttttttaat tcccctattt
     5341 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5401 tgcttcaata atattgaaaa aggaagagta tgagcattca gcattttcgt gtggcgctga
     5461 ttccgttttt tgcggcgttt tgcctgccgg tgtttgcgca tccggaaacc ctggtgaaag
     5521 tgaaagatgc ggaagatcaa ctgggtgcgc gcgtgggcta tattgaactg gatctgaaca
     5581 gcggcaaaat tctggaatct tttcgtccgg aagaacgttt tccgatgatg agcaccttta
     5641 aagtgctgct gtgcggtgcg gttctgagcc gtgtggatgc gggccaggaa caactgggcc
     5701 gtcgtattca ttatagccag aacgatctgg tggaatatag cccggtgacc gaaaaacatc
     5761 tgaccgatgg catgaccgtg cgtgaactgt gcagcgcggc gattaccatg agcgataaca
     5821 ccgcggcgaa cctgctgctg acgaccattg gcggtccgaa agaactgacc gcgtttctgc
     5881 ataacatggg cgatcatgtg acccgtctgg atcgttggga accggaactg aacgaagcga
     5941 ttccgaacga tgaacgtgat accaccatgc cggcagcaat ggcgaccacc ctgcgtaaac
     6001 tgctgacggg tgagctgctg accctggcaa gccgccagca actgattgat tggatggaag
     6061 cggataaagt ggcgggtccg ctgctgcgta gcgcgctgcc ggctggctgg tttattgcgg
     6121 ataaaagcgg tgcgggcgaa cgtggcagcc gtggcattat tgcggcgctg ggcccggatg
     6181 gtaaaccgag ccgtattgtg gtgatttata ccaccggcag ccaggcgacg atggatgaac
     6241 gtaaccgtca gattgcggaa attggcgcga gcctgattaa acattggtaa accgatacaa
     6301 ttaaaggctc cttttggagc cttttttttt ggacgaccct tgtcagatca aaggatcttc
//
