LOCUS       sequence-467649-        2689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Constitutive-Promoter_aacC1-Broad_PartType5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK049 [Con-Pro_aacC1-Broad_PartType5]
FEATURES             Location/Qualifiers
     source          1..2689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1396..1401,1402..1418,1419..1424)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1667..1758
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1759..1827,1828..2619)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2623..2662
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctaacgg cggtaggggt gccgcacggt tgcggcacca tgcgcaatca
      661 gctgcaactt ttcggcagcg cgacaacaat tatgcgttgc gtaaaagtgg cagtcaatta
      721 cagattttct ttaacctacg caatgagcta ttgcgggggg tgccgcaatg agctgttgcg
      781 tacccccctt ttttaagttg ttgattttta agtctttcgc atttcgccct atatctagtt
      841 ctttggtgcc caaagaaggg cacccctgcg gggttccccc acgccttcgg cgcggctccc
      901 cctccggcaa aaagtggccc ctccggggct tgttgatcga ctgcgcggcc ttcggccttg
      961 cccaaggtgg cgctgccccc ttggaacccc cgcactcgcc gccgtgaggc tcggggggca
     1021 ggcgggcggg cttcgccctt cgactgcccc cactcgcata ggcttgggtc gttccaggcg
     1081 cgtcaaggcc aagccgctgc gcggtcgctg cgcgagcctt gacccgcctt ccacttggtg
     1141 tccaaccggc aagcgaagcg cgcaggccgc aggccggagg cttttcccca gagaaaatta
     1201 aaaaaattga tggggcaagg ccgcaggccg cgcagttgga gccggtgggt atgaattcga
     1261 attcctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     1321 cagcaccttg tcgccttgcg tataatattt gcccatggac gcacaccgtg gaaacggatg
     1381 aaggcacgaa cccagttgac ataagcctgt tcggttcgta aactgtaatg caagtagcgt
     1441 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     1501 gcggttttca tggcttgtta tgactgtttt tttgtacagt ctatgcctcg ggcatccaag
     1561 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgtatgtgag
     1621 accagagtag cccgcctaat gagcgggctt ttttttaatt cccctatttg tttatttttc
     1681 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     1741 tattgaaaaa ggaagagtat gagcattcag cattttcgtg tggcgctgat tccgtttttt
     1801 gcggcgtttt gcctgccggt gtttgcgcat ccggaaaccc tggtgaaagt gaaagatgcg
     1861 gaagatcaac tgggtgcgcg cgtgggctat attgaactgg atctgaacag cggcaaaatt
     1921 ctggaatctt ttcgtccgga agaacgtttt ccgatgatga gcacctttaa agtgctgctg
     1981 tgcggtgcgg ttctgagccg tgtggatgcg ggccaggaac aactgggccg tcgtattcat
     2041 tatagccaga acgatctggt ggaatatagc ccggtgaccg aaaaacatct gaccgatggc
     2101 atgaccgtgc gtgaactgtg cagcgcggcg attaccatga gcgataacac cgcggcgaac
     2161 ctgctgctga cgaccattgg cggtccgaaa gaactgaccg cgtttctgca taacatgggc
     2221 gatcatgtga cccgtctgga tcgttgggaa ccggaactga acgaagcgat tccgaacgat
     2281 gaacgtgata ccaccatgcc ggcagcaatg gcgaccaccc tgcgtaaact gctgacgggt
     2341 gagctgctga ccctggcaag ccgccagcaa ctgattgatt ggatggaagc ggataaagtg
     2401 gcgggtccgc tgctgcgtag cgcgctgccg gctggctggt ttattgcgga taaaagcggt
     2461 gcgggcgaac gtggcagccg tggcattatt gcggcgctgg gcccggatgg taaaccgagc
     2521 cgtattgtgg tgatttatac caccggcagc caggcgacga tggatgaacg taaccgtcag
     2581 attgcggaaa ttggcgcgag cctgattaaa cattggtaaa ccgatacaat taaaggctcc
     2641 ttttggagcc tttttttttg gacgaccctt gtcagatcaa aggatcttc
//
