LOCUS       sequence-46768-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Gunhan E, Swe M, Palazoglu M, Voss JC, Chalupa LM
  TITLE     Expression and purification of cysteine introduced recombinant 
            saporin.
  JOURNAL   Protein Expr Purif. 2008 Apr;58(2):203-9. Epub 2007 Nov 19.
  PUBMED    18164211
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2008-04"; volume: "58(2)"; pages: "203-9. Epub 
            2007 Nov 19"
COMMENT     Sequence Label: pLysscys255
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(192..296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     364..382
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgcctg
       61 taaccnactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
      241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
      301 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
      361 aaaaataggc gtatcacgag gccctttcgt cttcaagaat tcctataact cgtccttttc
      421 gcaggccttt ggtttgccca aatacataag gagtcccatt tgcaagtcct tcacctgcct
      481 cacttttcca aacccgaaat cataatcttt attaaacacg ccgtttttgg catccccgta
      541 tattgccgta gaaatcttac gccagctgac ttcaaattga atcaccttgt tatccgagtc
      601 gaacttgttg gggaagttct tagttaccaa gttttgaatg tacctaaatc gtgctacctc
      661 agctgtcatt tgaatagcga taagcagaaa cctagcttcg tttttaacca cacgtgcctt
      721 cttgttcact gcttccatga acgtcaaaag taagtcgatc ccccaacccg agttcttttc
      781 tacttttatc tccctgtgtt atctggggca ttcttttcga tcgactgata atcttctgtg
      841 tattctaaag ctttctgatt tgcagttgtg gcctctggga aaagggcggg taactcggcg
      901 gaagtaattt ctgatttgaa gta
//
