LOCUS       sequence-467729-       10573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human codon optimized T4 DNA Ligase with N-terminal F0ATP MLS 
            expressing plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10573)
  AUTHORS   Fu Y, Land M, Cui R, Kavlashvili T, Kim M, Lieber T, Ryu KW, 
            DeBitetto E, Masilionis I, Saha R, Takizawa M, Baker D, Tigano M, 
            Reznik E, Sharma R, Chaligne R, Thompson CB, Pe'er D, Sfeir A
  TITLE     Engineering mtDNA Deletions by Reconstituting End-Joining in Human 
            Mitochondria.
  JOURNAL   bioRxiv [Preprint]. 2024 Oct 17:2024.10.15.618543. doi: 
            10.1101/2024.10.15.618543.
  PUBMED    39463974
REFERENCE   2  (bases 1 to 10573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Oct 17:2024.10.15.618543. doi: 
            10.1101/2024.10.15.618543."
COMMENT     Sequence Label: pHAGE2-EF1a-FOATPMLS-2xFLAG-T4Lig-IRES-Neo
FEATURES             Location/Qualifiers
     source          1..10573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    284..401
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(694..738)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    923..1156
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1653..1778
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1825..2458
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2650..2669)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2719..2735)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2719..2735)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2732..2754)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2743..2759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2767..2773,2774..2791,2792..2797))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2812..2833
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3073..3090)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3244..3832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(4003..4794,4795..4863))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4626..4645
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4864..4968)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5036..5054
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             5101..5281
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5429..6017
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5535..5546
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5944..5964
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6052..6846)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6163..6182)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6773..6792
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    6849..7400
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6851..7417
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7010..7029)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     7239..7256
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     RBS             8065..8073
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(9174..9221)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     promoter        complement(join(9631..10573,1..236))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(9640..10573,1..5)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(9663..9683)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cttacctgtg ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc gactactgca
       61 cttatatacg gttctccccc accctcggga aaaaggcgga gccagtacac gacatcactt
      121 tcccagttta ccccgcgcca ccttctctag gcaccggttc aattgccgac ccctcccccc
      181 aacttctcgg ggactgtggg cgatgtgcgc tctgcccact gacgggcacc ggagcctcac
      241 gactagtcca aactggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
      301 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
      361 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
      421 tttgtgaatt cggcgatacc gtcgagatcc gttcactaat cgaatggatc tgtctctgtc
      481 tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag
      541 gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac
      601 agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata attttatata
      661 ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa
      721 taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa
      781 gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg
      841 ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt
      901 tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag
      961 gattcttgcc tgaagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc
     1021 ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc
     1081 ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc
     1141 aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc
     1201 cttggtgggt gctactccta atggttcaat ttttactact ttatatttat ataattcact
     1261 tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc ggccgcttgc
     1321 tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg
     1381 tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat
     1441 ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag
     1501 ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc
     1561 catactatat gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc
     1621 catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc
     1681 tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc
     1741 gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct
     1801 ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga
     1861 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     1921 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     1981 agatctggtc taaccagaga gacccagtac aggcaaaaag cagctgctta tatgcaggat
     2041 ctgagggctc gccactcccc agtcccgccc aggccacgcc tccctggaaa gtccccagcg
     2101 gaaagtccct tgtagcaagc tcgatatcag cagttcttga agtactccgg atgcagctct
     2161 cgggccacgt gatgaaatgc taggcggctg tcaaacctcc actctaacac ttctctctcc
     2221 gggtcatcca tcccatgcag gctcacaggg tgtaacaagc tggtgttctc tcctttattg
     2281 gcctcttcta ccttatctgg ctcaactggt actagcttgt agcaccatcc aaaggtcagt
     2341 ggatatctga cccctggccc tggtgtgtag ttctgctaat cagggaagta gccttgtgtg
     2401 tggtagatcc acagatcaag gatatcttgt cttctttggg agtgaattag cccttccaac
     2461 tactaagttt gtagtacata tttaacaaat acaatttctt taaaatgaaa ataattcaga
     2521 ggaatcacag gtttagagta aatgaaacca caggtaattg gcagtggtaa tagggtatgg
     2581 ggtgggaagt ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata
     2641 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2701 tgtgaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2761 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2821 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2881 ccacggggag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga
     2941 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
     3001 gagcttgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3061 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3121 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3181 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3241 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3301 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3361 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3421 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3481 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3541 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3601 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3661 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3721 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3781 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3841 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3901 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3961 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4021 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4081 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4141 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4201 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4261 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4321 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4381 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4441 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4501 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4561 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
     4621 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4681 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4741 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4801 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4861 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4921 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4981 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     5041 gcgtatcacg aggccctttc gtcttcaaga atgatctagc cctttcctta atcgcccggg
     5101 tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac
     5161 acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca
     5221 gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc
     5281 cagtacaagc aaaaagcagg gcgcgcctgt cctgcaggtg atcttgtctt cgttgggagt
     5341 gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct
     5401 tgtaagtcat tggtcttaaa ggtaccaggc ggggaggcgg cccaaaggga gatccgactc
     5461 gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag gccgcgggaa
     5521 ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc
     5581 caggtggcaa cacaggcgag cagccaagga aaggacgatg atttccccga caacaccacg
     5641 gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca ggcggcgatg
     5701 agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg
     5761 gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac cacgccacgt
     5821 tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt atacaaggag
     5881 gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa agcagcgtat
     5941 ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc acaaattttg
     6001 taatccagag gttgattagg atctatcgat agatcccggt cggcatctac ttcagaagaa
     6061 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag
     6121 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa
     6181 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa
     6241 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc
     6301 ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg
     6361 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg
     6421 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag
     6481 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag
     6541 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac
     6601 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc
     6661 gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc
     6721 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc
     6781 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc
     6841 aatcatggta ttatcatcgt gtttttcaaa ggaaaaccac gtccccgtgg ttcggggggc
     6901 ctagacgttt tttaacctcg actaaacaca tgtaaagcat gtgcaccgag gccccagatc
     6961 agatcccata caatggggta ccttctgggc atccttcagc cccttgttga atacgcttga
     7021 ggagagccat ttgactcttt ccacaactat ccaactcaca acgtggcact ggggttgtgc
     7081 cgcctttgca ggtgtatctt atacacgtgg cttttggccg cagaggcacc tgtcgccagg
     7141 tggggggttc cgctgcctgc aaagggtcgc tacagacgtt gtttgtcttc aagaagcttc
     7201 cagaggaact gcttccttca cgacattcaa cagaccttgc attcctttgg cgagagggga
     7261 aagaccccta ggaatgctcg tcaagaagac agggccaggt ttccgggccc tcacattgcc
     7321 aaaagacggc aatatggtgg aaaataacat atagacaaac gcacaccggc cttattccaa
     7381 gcggcttcgg ccagtaacgt tagggggggg ggagggatcc gtttaaacgg gccctgaatt
     7441 cgattcaatc acacaaagca tttactattt tcaatcactt gcccaataac aaaatgttta
     7501 gtaagaaatt attcagaaca ttaagttgtt tatgaaataa gtgactaagc aacatcaaga
     7561 aatgctacaa tagagcagct tactgtattc tgcagtactc tataccacta caaaaacagt
     7621 cataaagagc ttaacatact cagcataacg atcgtggttt atctagaatc cagttccagc
     7681 tcctcgagtt ttttctgcag agcgctagcc agcccggtca cctcgtgaaa atcaccgaac
     7741 acgtcttcaa aagtattggc cttggtcttg tcttcccgca atctaatggc aatcggcaga
     7801 aacagcttta cgtagtctgt cctcccatca gacttaagcc atccattaca ctcacattca
     7861 agtatcttcc ctatataata attttggttc tccattattc gagtccggtc caattcgtga
     7921 gattttacgc ccgccttgtc cttcagccct gagcctgcat tcactttaat ctttccgcat
     7981 tcactctcca agatgaaccc tccagcttta gttgggtctt ttcgatgagg atatattcct
     8041 actatcttaa gatccacgtc aatcacctcc ttaaacttgt acaggttttt ggagcgtgcg
     8101 ttctcccaca acccgtcgat gttcttcagt ataatgcctt ccaaaccctg gtctatatat
     8161 tttttataaa tcaccttcgc ttcatccaga ttgttcacca cttgattttc aataagaatc
     8221 accttgtcat acccggacgt catttgctcc aatttagaaa agcgcacatc atacttgagt
     8281 ctgaaagccg gaagagaata gatttcaaca agcggtacgt aatcccacac ctggaacttc
     8341 atacactgag cttctttttc actgattgtg cctttgagac ttttatttgc aatgccattt
     8401 gatgccgttc gggactctgc tacttctgca aactcctttg ccttactgtt ctcaggataa
     8461 gcgtcaaaca aaaagtcgag tccctctggc tcttttttta cctgctcatg ataaaccagt
     8521 tcaccgtcaa taagtacgcc ttcagggtgt atttgccttg cttctgcagt catctttatc
     8581 aactcttcct tgagcaaatc caatccaagg tactcgtttc ctgctctgct caggagtctg
     8641 acatcatcca gttcatctcc ccggacctcc gcaaaacagc gtgctccatc tgctttgagt
     8701 tgagcaaagg cgggaaattt aatgtttttg ttaatcccct tttcgtcgta actactggca
     8761 agcatttgcg gttgctctgg tatcagaccg ggccaaacct tgttggcaat gcttactgac
     8821 gctccgcact ccagatctct catcattacc cttctaagga cctctacgtc atccttcttt
     8881 ccgtccgtta tgtagccggt aagctcctcg attgccgcat ttcctgtcaa ctttcgcgtg
     8941 gccagcgtga attcgatgaa atcaagcatg tcagtaagcg tgagcatacc gaaagattga
     9001 gtagcgattc ccggtttagg ccatttctta atataatatt gaagcccccg tgaataagtc
     9061 aggcggtaaa cccgcttcaa cagttcgtta tccttattct tttccagtat tgcttgcttt
     9121 tgtttggtac ttccaattga agctatctca ttcaagatct tgagaatgga tcccttgtcg
     9181 tcatcgtctt tgtagtcctt gtcatcgtcg tccttgtagt cgtcgacggt accatgaatg
     9241 gaagagtagg cgcgcttctg gaaagcctgg cgggtggtgg cgttgacgat ggaggtcatc
     9301 tgggtgcgct tgagggtctg gaggggggag ccagtctgga tggtgcgctt gctgacctga
     9361 gcaacgcgga cagcagggcg ggcaaccttg gcggaagcag ccatccggga ggccaggcga
     9421 gaggcgagga cacgagtgga ggccatacgc gtgctgctag tgctggtgct accgctgatg
     9481 ccgccgatct gctgaagccg ctgccgaagc caccacagcc acgagtgccg ctcctgcgcc
     9541 gcccgcgggc cttaagaaag cgcggggagc agggccgcga ccccagctgc gccgcaaggg
     9601 caccgcgaga aacttaatta agcggccgct tcacgacacc tgaaatggaa gaaaaaaact
     9661 ttgaaccact gtctgaggct tgagaatgaa ccaagatcca aactcaaaaa gggcaaattc
     9721 caaggagaat tacatcaagt gccaagctgg cctaacttca gtctccaccc actcagtgtg
     9781 gggaaactcc atcgcataaa acccctcccc ccaacctaaa gacgacgtac tccaaaagct
     9841 cgagaactaa tcgaggtgcc tggacggcgc ccggtactcc gtggagtcac atgaagcgac
     9901 ggctgaggac ggaaaggccc ttttcctttg tgtgggtgac tcacccgccc gctctcccga
     9961 gcgccgcgtc ctccattttg agctccctgc agcagggccg ggaagcggcc atctttccgc
    10021 tcacgcaact ggtgccgacc gggccagcct tgccgcccag ggcggggcga tacacggcgg
    10081 cgcgaggcca ggcaccagag caggccggcc agcttgagac tacccccgtc cgattctcgg
    10141 tggccgcgct cgcaggcccc gcctcgccga acatgtgcgc tgggacgcac gggccccgtc
    10201 gccgcccgcg gccccaaaaa ccgaaatacc agtgtgcaga tcttggcccg catttacaag
    10261 actatcttgc cagaaaaaaa gcgtcgcagc aggtcatcaa aaattttaaa tggctagaga
    10321 cttatcgaaa gcagcgagac aggcgcgaag gtgccaccag attcgcacgc ggcggcccca
    10381 gcgcccaggc caggcctcaa ctcaagcacg aggcgaaggg gctccttaag cgcaaggcct
    10441 cgaactctcc cacccacttc caacccgaag ctcgggatca agaatcacgt actgcagcca
    10501 ggtggaagta attcaaggca cgcaagggcc ataacccgta aagaggccag gcccgcggga
    10561 accacacacg gca
//
