LOCUS       sequence-467731-       10450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human codon optimized T4 DNA Ligase with N-terminal E1 MLS 
            expressing plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Fu Y, Land M, Cui R, Kavlashvili T, Kim M, Lieber T, Ryu KW, 
            DeBitetto E, Masilionis I, Saha R, Takizawa M, Baker D, Tigano M, 
            Reznik E, Sharma R, Chaligne R, Thompson CB, Pe'er D, Sfeir A
  TITLE     Engineering mtDNA Deletions by Reconstituting End-Joining in Human 
            Mitochondria.
  JOURNAL   bioRxiv [Preprint]. 2024 Oct 17:2024.10.15.618543. doi: 
            10.1101/2024.10.15.618543.
  PUBMED    39463974
REFERENCE   2  (bases 1 to 10450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Oct 17:2024.10.15.618543. doi: 
            10.1101/2024.10.15.618543."
COMMENT     Sequence Label: pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo
FEATURES             Location/Qualifiers
     source          1..10450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..40
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             204..998
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(258..277)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     868..887
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    1033..1621
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1086..1106)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1504..1515)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1769..1949
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1996..2014)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2082..2186
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2187..2255,2256..3047)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2405..2424)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3218..3806
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3960..3977
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4217..4238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4253..4258,4259..4276,4277..4283)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4291..4307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4296..4318
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4315..4331
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4315..4331
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4381..4400
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4592..5225
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5272..5397
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5894..6127
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6312..6356
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6649..6766
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6814..7992
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7045..7983
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7940..7960
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             8279..8326
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     RBS             9427..9435
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    join(10083..10450,1..199)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    join(10100..10450,1..201)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10244..10261)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
       61 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
      121 gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
      181 ttgaaaaaca cgatgataat accatgattg aacaagatgg attgcacgca ggttctccgg
      241 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
      301 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
      361 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
      421 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
      481 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
      541 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
      601 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
      661 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
      721 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
      781 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
      841 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
      901 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
      961 gcatcgcctt ctatcgcctt cttgacgagt tcttctgaag tagatgccga ccgggatcta
     1021 tcgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     1081 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     1141 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     1201 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     1261 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     1321 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     1381 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     1441 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     1501 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     1561 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     1621 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     1681 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc acctgcagga
     1741 caggcgcgcc ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     1801 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     1861 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     1921 cttttagtca gtgtggaaaa tctctagcac ccgggcgatt aaggaaaggg ctagatcatt
     1981 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     2041 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2101 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2161 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2221 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2281 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2341 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2401 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     2461 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2521 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2581 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2641 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2701 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     2761 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2821 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2881 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     2941 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3001 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3061 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3121 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3181 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3241 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3301 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3361 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3421 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3481 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3541 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3601 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3661 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3721 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3781 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3841 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3901 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3961 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4021 gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga
     4081 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4141 cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca
     4201 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     4261 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     4321 acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt
     4381 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
     4441 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
     4501 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
     4561 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
     4621 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
     4681 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
     4741 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
     4801 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
     4861 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
     4921 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
     4981 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
     5041 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     5101 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     5161 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     5221 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     5281 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     5341 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     5401 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     5461 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     5521 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     5581 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     5641 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     5701 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
     5761 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     5821 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     5881 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     5941 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     6001 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     6061 ggggcatcaa gcagcttcag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     6121 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     6181 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     6241 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     6301 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     6361 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     6421 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     6481 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     6541 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     6601 gatctcgacg gtatcgccga attcacaaat ggcagtattc atccacaatt ttaaaagaaa
     6661 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     6721 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     6781 ggacagcaga gatccagttt ggactagtcg tgaggctccg gtgcccgtca gtgggcagag
     6841 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     6901 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     6961 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     7021 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     7081 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     7141 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     7201 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     7261 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     7321 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     7381 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     7441 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     7501 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     7561 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     7621 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     7681 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     7741 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     7801 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     7861 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     7921 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     7981 caggtgtcgt gaagcggccg cttaattaag tttctcgcgg tgcccttgcg gcgcagctgg
     8041 ggtcgcggcc ctgctccccg cgctttctta aggcccgcgg gcggcgcagg agcggcactc
     8101 gtggctgtgg tggcttcggc agcggcttca gcagatcggc ggcatcagcg gtagcaccag
     8161 cactagcagc atgagaaaaa tgctggctgc cgtgagccga gtgctgtccg gggcaagtca
     8221 aaagccagcc tcaagagtct tggtggcaag tagaaatttc attcatggta ccgtcgacga
     8281 ctacaaggac gacgatgaca aggactacaa agacgatgac gacaagggat ccattctcaa
     8341 gatcttgaat gagatagctt caattggaag taccaaacaa aagcaagcaa tactggaaaa
     8401 gaataaggat aacgaactgt tgaagcgggt ttaccgcctg acttattcac gggggcttca
     8461 atattatatt aagaaatggc ctaaaccggg aatcgctact caatctttcg gtatgctcac
     8521 gcttactgac atgcttgatt tcatcgaatt cacgctggcc acgcgaaagt tgacaggaaa
     8581 tgcggcaatc gaggagctta ccggctacat aacggacgga aagaaggatg acgtagaggt
     8641 ccttagaagg gtaatgatga gagatctgga gtgcggagcg tcagtaagca ttgccaacaa
     8701 ggtttggccc ggtctgatac cagagcaacc gcaaatgctt gccagtagtt acgacgaaaa
     8761 ggggattaac aaaaacatta aatttcccgc ctttgctcaa ctcaaagcag atggagcacg
     8821 ctgttttgcg gaggtccggg gagatgaact ggatgatgtc agactcctga gcagagcagg
     8881 aaacgagtac cttggattgg atttgctcaa ggaagagttg ataaagatga ctgcagaagc
     8941 aaggcaaata caccctgaag gcgtacttat tgacggtgaa ctggtttatc atgagcaggt
     9001 aaaaaaagag ccagagggac tcgacttttt gtttgacgct tatcctgaga acagtaaggc
     9061 aaaggagttt gcagaagtag cagagtcccg aacggcatca aatggcattg caaataaaag
     9121 tctcaaaggc acaatcagtg aaaaagaagc tcagtgtatg aagttccagg tgtgggatta
     9181 cgtaccgctt gttgaaatct attctcttcc ggctttcaga ctcaagtatg atgtgcgctt
     9241 ttctaaattg gagcaaatga cgtccgggta tgacaaggtg attcttattg aaaatcaagt
     9301 ggtgaacaat ctggatgaag cgaaggtgat ttataaaaaa tatatagacc agggtttgga
     9361 aggcattata ctgaagaaca tcgacgggtt gtgggagaac gcacgctcca aaaacctgta
     9421 caagtttaag gaggtgattg acgtggatct taagatagta ggaatatatc ctcatcgaaa
     9481 agacccaact aaagctggag ggttcatctt ggagagtgaa tgcggaaaga ttaaagtgaa
     9541 tgcaggctca gggctgaagg acaaggcggg cgtaaaatct cacgaattgg accggactcg
     9601 aataatggag aaccaaaatt attatatagg gaagatactt gaatgtgagt gtaatggatg
     9661 gcttaagtct gatgggagga cagactacgt aaagctgttt ctgccgattg ccattagatt
     9721 gcgggaagac aagaccaagg ccaatacttt tgaagacgtg ttcggtgatt ttcacgaggt
     9781 gaccgggctg gctagcgctc tgcagaaaaa actcgaggag ctggaactgg attctagata
     9841 aaccacgatc gttatgctga gtatgttaag ctctttatga ctgtttttgt agtggtatag
     9901 agtactgcag aatacagtaa gctgctctat tgtagcattt cttgatgttg cttagtcact
     9961 tatttcataa acaacttaat gttctgaata atttcttact aaacattttg ttattgggca
    10021 agtgattgaa aatagtaaat gctttgtgtg attgaatcga attcagggcc cgtttaaacg
    10081 gatccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
    10141 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
    10201 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
    10261 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
    10321 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
    10381 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
    10441 gtgagttgga
//
