LOCUS       sequence-467732-       10011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human codon optimized Ku (Mycobacterium) with N-terminal E1 MLS 
            expressing plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   Fu Y, Land M, Cui R, Kavlashvili T, Kim M, Lieber T, Ryu KW, 
            DeBitetto E, Masilionis I, Saha R, Takizawa M, Baker D, Tigano M, 
            Reznik E, Sharma R, Chaligne R, Thompson CB, Pe'er D, Sfeir A
  TITLE     Engineering mtDNA Deletions by Reconstituting End-Joining in Human 
            Mitochondria.
  JOURNAL   bioRxiv [Preprint]. 2024 Oct 17:2024.10.15.618543. doi: 
            10.1101/2024.10.15.618543.
  PUBMED    39463974
REFERENCE   2  (bases 1 to 10011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Oct 17:2024.10.15.618543. doi: 
            10.1101/2024.10.15.618543."
COMMENT     Sequence Label: pHAGE2-EF1a-E1MLS-2xMyc-Ku-IRES-Hygro
FEATURES             Location/Qualifiers
     source          1..10011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    814..1402
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(867..887)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1285..1296)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1550..1730
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1777..1795)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1863..1967
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1968..2036,2037..2828)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2186..2205)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2999..3587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3741..3758
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3998..4019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4034..4039,4040..4057,4058..4064)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4072..4088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4077..4099
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4096..4112
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4096..4112
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4162..4181
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4373..5006
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5053..5178
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5675..5908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6093..6137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6430..6547
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6595..7773
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6826..7764
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7721..7741
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             8060..8089
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             8096..8125
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    9299..9760
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9355..9372)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9582..9601
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9765..10011,1..779)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             join(9765..10011,1..779)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
       61 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
      121 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
      181 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
      241 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
      301 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
      361 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
      421 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
      481 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
      541 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
      601 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
      661 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
      721 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga
      781 gtagatgccg accgggatct atcgatagat cctaatcaac ctctggatta caaaatttgt
      841 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
      901 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
      961 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     1021 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     1081 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     1141 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     1201 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     1261 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     1321 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     1381 tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
     1441 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     1501 aagacaagat cacctgcagg acaggcgcgc cctgcttttt gcttgtactg ggtctctctg
     1561 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     1621 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     1681 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca cccgggcgat
     1741 taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     1801 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     1861 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1921 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     1981 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2041 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     2101 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     2161 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     2221 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2281 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     2341 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     2401 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     2461 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     2521 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     2581 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     2641 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     2701 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     2761 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2821 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2881 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2941 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3001 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3061 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3121 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3181 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3241 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3301 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3361 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3421 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3481 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3541 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3601 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3661 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3721 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3781 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact
     3841 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3901 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt
     3961 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     4021 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     4081 gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat
     4141 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     4201 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
     4261 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
     4321 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
     4381 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     4441 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     4501 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     4561 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     4621 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     4681 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     4741 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     4801 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     4861 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4921 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     4981 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     5041 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     5101 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     5161 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     5221 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     5281 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     5341 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     5401 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     5461 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     5521 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     5581 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     5641 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     5701 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     5761 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     5821 atctgttgca actcacagtc tggggcatca agcagcttca ggcaagaatc ctggctgtgg
     5881 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     5941 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     6001 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     6061 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     6121 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     6181 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     6241 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     6301 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     6361 gatccattcg attagtgaac ggatctcgac ggtatcgccg aattcacaaa tggcagtatt
     6421 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     6481 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     6541 aaattttcgg gtttattaca gggacagcag agatccagtt tggactagtc gtgaggctcc
     6601 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     6661 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     6721 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     6781 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     6841 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     6901 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
     6961 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     7021 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     7081 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     7141 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
     7201 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     7261 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     7321 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     7381 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     7441 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     7501 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     7561 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     7621 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     7681 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     7741 caaagttttt ttcttccatt tcaggtgtcg tgaagcggcc gcttaattaa gtttctcgcg
     7801 gtgcccttgc ggcgcagctg gggtcgcggc cctgctcccc gcgctttctt aaggcccgcg
     7861 ggcggcgcag gagcggcact cgtggctgtg gtggcttcgg cagcggcttc agcagatcgg
     7921 cggcatcagc ggtagcacca gcactagcag catgagaaaa atgctggctg ccgtgagccg
     7981 agtgctgtcc ggggcaagtc aaaagccagc ctcaagagtc ttggtggcaa gtagaaattt
     8041 cattcatggt accgtcgacg aacagaaact catcagtgaa gaggatttgg gcagcgaaca
     8101 gaaacttatc agcgaagaag acctgggatc cagggccatt tggacaggtt ccatagcatt
     8161 tggcctggta aacgtcccag taaaggtgta ctctgctaca gcagaccatg atataaggtt
     8221 ccatcaggta catgccaaag ataacggtcg gataaggtac aagcgggttt gtgaagcttg
     8281 tggtgaagta gtagattacc gcgatttggc aagagcttac gaatcaggtg acggtcagat
     8341 ggtggcaatt actgacgacg acatcgcctc tctgccggag gaaagatcca gggagatcga
     8401 ggtgttggaa ttcgtcccag cggcagatgt tgatccgatg atgtttgacc gatcctattt
     8461 tcttgaaccg gactctaaga gttcaaaatc ttatgtgttg cttgcaaaga cgctcgctga
     8521 aacagatagg atggcgattg tgcatttcac tctcagaaac aaaacacggc ttgctgcgct
     8581 gcgcgtgaag gacttcggaa agcgagaggt gatgatggta cataccctgc tgtggcctga
     8641 cgagatccgc gatcccgact ttccggttct ggaccagaag gtcgagatta aacccgctga
     8701 gctgaagatg gcgggtcaag tcgtcgatag catggcagac gattttaatc ctgacagata
     8761 tcatgacacc tatcaagagc agttgcaaga attgatcgat acgaaactgg aggggggcca
     8821 ggccttcacc gcagaggatc agccgcggct cctggatgag cctgaagatg tgagcgactt
     8881 gctcgcaaaa ctggaggcct cagtcaaagc aaggtcaaag gcgaattcta acgttccaac
     8941 gccccctgct agcgctctgc agaaaaaact cgaggagctg gaactggatt ctagataaac
     9001 cacgatcgtt atgctgagta tgttaagctc tttatgactg tttttgtagt ggtatagagt
     9061 actgcagaat acagtaagct gctctattgt agcatttctt gatgttgctt agtcacttat
     9121 ttcataaaca acttaatgtt ctgaataatt tcttactaaa cattttgtta ttgggcaagt
     9181 gattgaaaat agtaaatgct ttgtgtgatt gaatcgaatt cagggcccgt ttgtatctag
     9241 agctcgaggt cgaccaattc cggttatttt ccaccatatt gccgtctttt ggcaatgtga
     9301 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     9361 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     9421 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     9481 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     9541 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     9601 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     9661 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa
     9721 ccacggggac gtggttttcc tttgaaaaac acgatgataa taccatgaaa aagcctgaac
     9781 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     9841 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     9901 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     9961 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa t
//
