LOCUS       sequence-467734-        3931 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConLS/R1 cassette; rtTA systems; pRNR2-rtTA-v16-tADH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO246
FEATURES             Location/Qualifiers
     source          1..3931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     339..358
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(577..681)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      784..1372
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1273..1292
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      3143..3330
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3198..3222)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(3647..3931,1..507,508..576))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
       61 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
      121 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
      181 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
      241 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
      301 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
      361 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
      421 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
      481 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
      541 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
      601 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
      661 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgtca tgaccaaaat
      721 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
      781 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      841 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
      901 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
      961 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1021 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1081 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1141 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1201 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1261 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1321 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1381 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1441 tgcgttatcc cctgattctg tggataaccg tgcggccgcc cctgaattcg catctagatg
     1501 gtagagccac aaacagccgg tacaagcaac gatctccagg accatctgaa tcatgcgcgg
     1561 atgacacgaa ctcacgacgg cgatcacaga cattaaccca cagtacagac actgcgacaa
     1621 cgtggcaatt cgtcgcaata ccgtctcact gaactggccg ataattgcag acgaacgagt
     1681 cgaacaagaa gcaggcaaag tttagagcac tgcccctccg cactcaaaaa agaaaaaact
     1741 aggaggaaaa taaaattctc aaccacacaa acacataaac acatacaaat acaaatacaa
     1801 gcttatttac ttgacatcgc gcgatcttcc actattcagc gccgtccgcc ctctctcgtg
     1861 ttttttgttt acgcgacaac tatgcgaaat ccggagcaac gggcaaccgt ttggggaaag
     1921 accacaccca cgcgcgatcg ccatggcaac gaggtcgcac acgccccaca cccagacctc
     1981 cctgcgagcg ggcatgggta caatgtcccc gttgccacag acaccacttc gtagcacagc
     2041 gcagagcgta gcgtgttgtt gctgctgaca aaagaaaatt tttcttagca aagcaaagga
     2101 ggggaagcac gggcagatag caccgtacca tacccttgga aactcgaaat gaacgaagca
     2161 ggaaatgaga gaatgagagt tttgtaggta tatatagcgg tagtgtttgc gcgttaccat
     2221 catcttctgg atctatctat tgttcttttc ctcatcactt tccccttttt cgctcttctt
     2281 cttgtctttt atttctttct tttttttaat tgttccctcg attggctatc taccaaagaa
     2341 tccaaactta atacacgtat ttatttgtcc aattaccaga tctatgagtc gtcttgataa
     2401 gtccaagatc ataaatagcg cactggaatt gctgaacggc gttggtattg agggtcttac
     2461 cacgagaaag ctggcacaga agttgggggt cgaacaaccg accttgtact ggcatgtcaa
     2521 gaataagcgt gcgctattag atgccttacc tatcgagatg ctagacagac atcatactca
     2581 ctcatgtcca ttggaggggg agtcttggca agactttctt agaaacaatg caaagtcata
     2641 taggtgcgcg ctactatcac atagagatgg ggccaaagtc catttgggca ccaggccgac
     2701 cgaaaagcag tacgagactt tagagaacca gctggcattc ctgtgtcaac aaggcttcag
     2761 tctagagaat gctctgtatg ctttaagcgc ggtcggccac ttcacgctag gttgtgtatt
     2821 agaggaacaa gaacaccaag tggcaaaaga ggaaagagaa actccaacga ccgacagcat
     2881 gccaccactg ttgaaacaag caatcgaact gtttgatcgt caaggggctg aaccagcatt
     2941 cctattcggt ttggaattaa taatctgcgg tctggagaaa caattgaagt gcgaaagcgg
     3001 cgggccgaca gatgccttag atgactttga cctagatatg ttaccagccg acgcccttga
     3061 cgactttgat ctggacatgc tgccggctga cgccttggat gacttcgact tggatatgtt
     3121 gccaggggga tcctaactcg aggcgaattt cttatgattt atgattttta ttattaaata
     3181 agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa
     3241 attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga
     3301 ggtcgctctt attgaccaca cctctaccgg catgccgagc aaatgcctgc aaatcgctcc
     3361 ccatttcgct gccaatgaga cgacggggtc atcacggctc atcatgcgcc aaacaaatgt
     3421 gtgcaataca cgctcggatg actgcatgat gaccgcactg actggggaca gcagatccac
     3481 ctaagcctgt gagagaagca gacacccgac agatcaaggc agttaactag tgcactgcag
     3541 tacagcggcc gcgattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3601 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3661 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3721 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3781 taccgcggga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3841 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3901 gccgggaagc tagagtaagt agttcgccag t
//
